Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02220
(475 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P37167|ACTP_ACACA Actophorin 93 3e-19
sp|P45594|CADF_DROME Cofilin/actin depolymerizing factor ho... 93 4e-19
sp|Q03048|COFI_YEAST Cofilin 87 3e-17
sp|P78929|COFI_SCHPO Cofilin 86 4e-17
sp|Q9ZSK3|ADF4_ARATH Actin-depolymerizing factor 4 (ADF-4) ... 82 5e-16
sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g0... 81 1e-15
sp|Q9FVI2|ADF1_PETHY Actin-depolymerizing factor 1 (ADF 1) 79 7e-15
sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) ... 78 1e-14
sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2) 77 2e-14
sp|P54706|COFI_DICDI Cofilin 75 6e-14
>sp|P37167|ACTP_ACACA Actophorin
Length = 138
Score = 93.2 bits (230), Expect = 3e-19
Identities = 43/130 (33%), Positives = 77/130 (59%), Gaps = 3/130 (2%)
Frame = +2
Query: 5 NCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLNDACAQKRGLYA 184
+C + + E+K+G +HRYV F + + + V +ATYEDF + L + + YA
Sbjct: 9 DCVQKFNELKLGHQHRYVTFKMNASNTEVVVEHVGGPNATYEDFKSQLPERDCR----YA 64
Query: 185 VYEYEISEKNSQ---LIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVDEI 355
+++YE Q + F++W PD P+++KM+Y +K+ ++K+LVGI+ ++A D EI
Sbjct: 65 IFDYEFQVDGGQRNKITFILWAPDSAPIKSKMMYTSTKDSIKKKLVGIQVEVQATDAAEI 124
Query: 356 SKEEIEARLK 385
S++ + R K
Sbjct: 125 SEDAVSERAK 134
>sp|P45594|CADF_DROME Cofilin/actin depolymerizing factor homolog (D61 protein) (Twinstar
protein)
Length = 148
Score = 92.8 bits (229), Expect = 4e-19
Identities = 51/137 (37%), Positives = 77/137 (56%), Gaps = 9/137 (6%)
Frame = +2
Query: 8 CAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLNDACAQKRGLYAV 187
C Y EIK +KHRYV+F I + +K IDV A R+A Y+ F+ D+ C Y +
Sbjct: 11 CKTTYEEIKKDKKHRYVIFYIRD-EKQIDVETVADRNAEYDQFLEDIQK-CGPGECRYGL 68
Query: 188 YEYEI---------SEKNSQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEAN 340
+++E S K +L + W PD V+ KMLY+ S + L+K LVG++ I+A
Sbjct: 69 FDFEYMHQCQGTSESSKKQKLFLMSWCPDTAKVKKKMLYSSSFDALKKSLVGVQKYIQAT 128
Query: 341 DVDEISKEEIEARLK*T 391
D+ E S+E +E +L+ T
Sbjct: 129 DLSEASREAVEEKLRAT 145
>sp|Q03048|COFI_YEAST Cofilin
Length = 143
Score = 86.7 bits (213), Expect = 3e-17
Identities = 47/129 (36%), Positives = 78/129 (60%), Gaps = 7/129 (5%)
Frame = +2
Query: 17 AYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADL--NDACAQKRGLYAVY 190
A+ ++K+G+K++++LFG+ + I V K+ D +Y+ F+ L ND LYA+Y
Sbjct: 15 AFNDLKLGKKYKFILFGLNDAKTEI-VVKETSTDPSYDAFLEKLPENDC------LYAIY 67
Query: 191 --EYEISE---KNSQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVDEI 355
EYEI+ K S+++F W PD PVR+KM+YA SK+ LR+ L G+ ++ D E+
Sbjct: 68 DFEYEINGNEGKRSKIVFFTWSPDTAPVRSKMVYASSKDALRRALNGVSTDVQGTDFSEV 127
Query: 356 SKEEIEARL 382
S + + R+
Sbjct: 128 SYDSVLERV 136
>sp|P78929|COFI_SCHPO Cofilin
Length = 137
Score = 85.9 bits (211), Expect = 4e-17
Identities = 45/126 (35%), Positives = 76/126 (60%), Gaps = 3/126 (2%)
Frame = +2
Query: 2 PNCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLNDACAQKRGLY 181
P C EA+ E+K+G+ RYV+F + + I V KK+ D ++ F+ DL +K Y
Sbjct: 10 PECLEAFQELKLGKSLRYVVFKMNDTKTEIVVEKKST-DKDFDTFLGDL----PEKDCRY 64
Query: 182 AVYEYEIS---EKNSQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVDE 352
A+Y++E + +++IF+ W PDV P+++KM+Y+ SK+ LR+ GI I+A D E
Sbjct: 65 AIYDFEFNLGEGVRNKIIFISWSPDVAPIKSKMVYSSSKDTLRRAFTGIGTDIQATDFSE 124
Query: 353 ISKEEI 370
++ E +
Sbjct: 125 VAYETV 130
>sp|Q9ZSK3|ADF4_ARATH Actin-depolymerizing factor 4 (ADF-4) (AtADF4)
Length = 139
Score = 82.4 bits (202), Expect = 5e-16
Identities = 45/131 (34%), Positives = 78/131 (59%), Gaps = 5/131 (3%)
Frame = +2
Query: 5 NCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLN-DACAQKRGLY 181
+C + E+K R HR++++ I E K + V K TYEDF A L D C Y
Sbjct: 13 DCKLRFLELKAKRTHRFIVYKIEEKQKQVIVEKVGEPILTYEDFAASLPADECR-----Y 67
Query: 182 AVYEYE-ISEKN---SQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVD 349
A+Y+++ ++ +N S++ F+ W PDV VR+KM+YA SK+ +++L GI+ ++A D
Sbjct: 68 AIYDFDFVTAENCQKSKIFFIAWCPDVAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 127
Query: 350 EISKEEIEARL 382
E+ + +++R+
Sbjct: 128 EMDLDVLKSRV 138
>sp|Q9LQ81|ADFX_ARATH Actin-depolymerizing factor like At1g01750 (ADF-like)
Length = 140
Score = 80.9 bits (198), Expect = 1e-15
Identities = 44/130 (33%), Positives = 74/130 (56%), Gaps = 5/130 (3%)
Frame = +2
Query: 8 CAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADL-NDACAQKRGLYA 184
C + E+K R +R+++F I E + + + K + TYEDF + D C YA
Sbjct: 14 CKLKFLELKAKRNYRFIVFKIDEKAQQVMIDKLGNPEETYEDFTRSIPEDECR-----YA 68
Query: 185 VYEYEISE----KNSQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVDE 352
VY+Y+ + + S++ F+ W PD VR+KMLYA SK+ +++L GI+ ++A D E
Sbjct: 69 VYDYDFTTPENCQKSKIFFIAWSPDTSRVRSKMLYASSKDRFKRELDGIQVELQATDPSE 128
Query: 353 ISKEEIEARL 382
+S + I+ R+
Sbjct: 129 MSLDIIKGRV 138
>sp|Q9FVI2|ADF1_PETHY Actin-depolymerizing factor 1 (ADF 1)
Length = 139
Score = 78.6 bits (192), Expect = 7e-15
Identities = 43/129 (33%), Positives = 75/129 (58%), Gaps = 4/129 (3%)
Frame = +2
Query: 5 NCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLNDACAQKRGLYA 184
+C + E+K R HR++++ I E K + V K +YEDF A L + YA
Sbjct: 13 DCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKIGEPTESYEDFAASL----PENECRYA 68
Query: 185 VYEYE-ISEKN---SQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVDE 352
VY+++ ++ +N S++ F+ W PD VR+KM+YA SK+ +++L GI+ ++A D E
Sbjct: 69 VYDFDFVTAENCQKSRIFFIAWCPDTARVRSKMIYASSKDRFKRELDGIQVELQACDPTE 128
Query: 353 ISKEEIEAR 379
+ + I++R
Sbjct: 129 MGLDVIQSR 137
>sp|Q39250|ADF1_ARATH Actin-depolymerizing factor 1 (ADF-1) (AtADF1)
Length = 139
Score = 77.8 bits (190), Expect = 1e-14
Identities = 43/130 (33%), Positives = 75/130 (57%), Gaps = 5/130 (3%)
Frame = +2
Query: 5 NCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLN-DACAQKRGLY 181
+C + E+K R HR++++ I E K + V K TYE+F A L D C Y
Sbjct: 13 DCKLRFLELKAKRTHRFIVYKIEEKQKQVVVEKVGQPIQTYEEFAACLPADECR-----Y 67
Query: 182 AVYEYE-ISEKN---SQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVD 349
A+Y+++ ++ +N S++ F+ W PD+ VR+KM+YA SK+ +++L GI+ ++A D
Sbjct: 68 AIYDFDFVTAENCQKSKIFFIAWCPDIAKVRSKMIYASSKDRFKRELDGIQVELQATDPT 127
Query: 350 EISKEEIEAR 379
E+ + +R
Sbjct: 128 EMDLDVFRSR 137
>sp|Q9FVI1|ADF2_PETHY Actin-depolymerizing factor 2 (ADF 2)
Length = 143
Score = 77.0 bits (188), Expect = 2e-14
Identities = 43/130 (33%), Positives = 71/130 (54%), Gaps = 5/130 (3%)
Frame = +2
Query: 5 NCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLN-DACAQKRGLY 181
+C + E+K R +R++++ I E K + V K +YEDF A L D C Y
Sbjct: 13 DCKLKFLELKAKRTYRFIIYKIEEKQKEVVVEKLGEPTESYEDFTAGLPADECR-----Y 67
Query: 182 AVYEYEISEKN----SQLIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVD 349
AVY+++ K S++ F+ W PD VR+KM+YA SK+ +++L GI+ ++A D
Sbjct: 68 AVYDFDFMTKENHQKSRIFFIAWSPDTARVRSKMIYASSKDRFKRELDGIQVELQATDPT 127
Query: 350 EISKEEIEAR 379
E+ + +R
Sbjct: 128 EMGLDVFRSR 137
>sp|P54706|COFI_DICDI Cofilin
Length = 137
Score = 75.5 bits (184), Expect = 6e-14
Identities = 37/121 (30%), Positives = 64/121 (52%), Gaps = 3/121 (2%)
Frame = +2
Query: 2 PNCAEAYGEIKMGRKHRYVLFGITEGDKLIDVTKKAPRDATYEDFVADLNDACAQKRGLY 181
PNC + ++K+GRK+ +++ I++ K I V P ++++F L + Y
Sbjct: 9 PNCVSTFNDLKLGRKYGGIIYRISDDSKEIIVDSTLPAGCSFDEFTKCL----PENECRY 64
Query: 182 AVYEYEISEKNSQ---LIFVVWIPDVIPVRAKMLYAGSKEGLRKQLVGIKAVIEANDVDE 352
V +Y+ E+ +Q + FV W PD ++ KM+ SK+ LRK VGI+ I+ D E
Sbjct: 65 VVLDYQYKEEGAQKSKICFVAWCPDTANIKKKMMATSSKDSLRKACVGIQVEIQGTDASE 124
Query: 353 I 355
+
Sbjct: 125 V 125
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,543,563
Number of Sequences: 369166
Number of extensions: 733915
Number of successful extensions: 2308
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2175
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2274
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2783017720
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)