Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02188
(763 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P22907|HEM3_MOUSE Porphobilinogen deaminase (Hydroxymeth... 104 2e-22
sp|P19356|HEM3_RAT Porphobilinogen deaminase (Hydroxymethyl... 103 4e-22
sp|Q7V2I1|HEM3_PROMP Porphobilinogen deaminase (PBG) (Hydro... 103 4e-22
sp|Q6CT60|HEM3_KLULA Porphobilinogen deaminase (PBG) (Hydro... 103 4e-22
sp|P28789|HEM3_YEAST Porphobilinogen deaminase (PBG) (Hydro... 101 2e-21
sp|Q8DIE4|HEM3_SYNEL Porphobilinogen deaminase (PBG) (Hydro... 101 3e-21
sp|P73660|HEM3_SYNY3 Porphobilinogen deaminase (PBG) (Hydro... 100 3e-21
sp|P08397|HEM3_HUMAN Porphobilinogen deaminase (Hydroxymeth... 100 7e-21
sp|Q8YVU6|HEM3_ANASP Porphobilinogen deaminase (PBG) (Hydro... 99 1e-20
sp|Q6FNR4|HEM3_CANGA Porphobilinogen deaminase (PBG) (Hydro... 99 1e-20
>sp|P22907|HEM3_MOUSE Porphobilinogen deaminase (Hydroxymethylbilane synthase) (HMBS)
(Pre-uroporphyrinogen synthase) (PBG-D)
Length = 361
Score = 104 bits (260), Expect = 2e-22
Identities = 70/210 (33%), Positives = 107/210 (50%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
++LA AGL+R+G ++R+ Q+L E +YAVGQGALA E R+ D + L L+S +HD E+ +
Sbjct: 186 IVLAVAGLQRMGWQNRVGQILHPEECMYAVGQGALAVEVRAKDQDILDLVSVLHDPETLL 245
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDEISALLPEEDI 375
CIAER+ +R L+GGCS P+AV + + +DG L L V + G++ + + + A +
Sbjct: 246 RCIAERAFLRHLEGGCSVPVAVHT-VIKDGQLYLTGGVWSLDGSDSMQETMQATIQVP-- 302
Query: 376 ECFLERIQSSDNEENDEYPPKKKLKRQNLLTACPITDVDIQLGKDANAVFLGAQVFPICE 555
+Q D E+D +G A + GAQ+
Sbjct: 303 ------VQQEDGPEDDPQ----------------------LVGITARNIPRGAQL----- 329
Query: 556 IGRMRMAISQRVGAELAHRLLEAGAKRILD 645
++ +G LA LL GAK ILD
Sbjct: 330 -------AAENLGISLASLLLNKGAKNILD 352
>sp|P19356|HEM3_RAT Porphobilinogen deaminase (Hydroxymethylbilane synthase) (HMBS)
(Pre-uroporphyrinogen synthase) (PBG-D)
Length = 361
Score = 103 bits (258), Expect = 4e-22
Identities = 70/210 (33%), Positives = 106/210 (50%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
+ILA AGL+R+G ++R+ Q+L E +YAVGQGALA E R+ D + L L+ +HD E+ +
Sbjct: 186 IILAVAGLQRMGWQNRVGQILHPEECMYAVGQGALAVEVRAKDQDILDLVGVLHDPETLL 245
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDEISALLPEEDI 375
CIAER+ +R L+GGCS P+AV + + +DG L L V + G++ + + + A +
Sbjct: 246 RCIAERAFLRHLEGGCSVPVAVHT-VMKDGQLYLTGGVWSLDGSDSMQETMQATIQVP-- 302
Query: 376 ECFLERIQSSDNEENDEYPPKKKLKRQNLLTACPITDVDIQLGKDANAVFLGAQVFPICE 555
+Q D E+D +G A + GAQ+
Sbjct: 303 ------VQQEDGPEDDPQ----------------------LVGITARNIPRGAQL----- 329
Query: 556 IGRMRMAISQRVGAELAHRLLEAGAKRILD 645
++ +G LA LL GAK ILD
Sbjct: 330 -------AAENLGISLASLLLNKGAKNILD 352
>sp|Q7V2I1|HEM3_PROMP Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 316
Score = 103 bits (258), Expect = 4e-22
Identities = 54/110 (49%), Positives = 72/110 (65%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
+ILA AGL+R+G ESR+ Q++ E SL+AVGQGAL EC+SDD LK++S + D S
Sbjct: 170 IILAAAGLKRLGFESRVHQIIPNEISLHAVGQGALGIECKSDDKEVLKIISVLEDKVSSQ 229
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDE 345
C+AERS +R+L+GGC PI V S+I D I L V + G I +E
Sbjct: 230 RCLAERSFLRELEGGCQVPIGVNSSIQNDEI-ALIGMVASIDGKRLIKNE 278
>sp|Q6CT60|HEM3_KLULA Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 327
Score = 103 bits (258), Expect = 4e-22
Identities = 59/126 (46%), Positives = 76/126 (60%), Gaps = 4/126 (3%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
++LA AGL R GL+SRI+Q + + YAVGQGAL E R DD K+L I D + I
Sbjct: 176 IVLASAGLMRSGLDSRITQRFNADTMCYAVGQGALGIEIRKDDEKMKKILKEICDPSTTI 235
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISED-GILTLRAAVMNTSGTECISDEISALLP--- 363
C+AERSL+R L+GGCS PI V SN E +LTL+ V+N GTE + E +
Sbjct: 236 CCLAERSLLRTLEGGCSVPIGVVSNYDESTKVLTLKGIVINVEGTEWVEIEHKVTISNER 295
Query: 364 EEDIEC 381
E+ I C
Sbjct: 296 EDSINC 301
>sp|P28789|HEM3_YEAST Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 327
Score = 101 bits (252), Expect = 2e-21
Identities = 57/126 (45%), Positives = 79/126 (62%), Gaps = 4/126 (3%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
+ILA AGL R+GLE+RI+Q + +AVGQGAL E R D +K+L I D + I
Sbjct: 176 IILASAGLMRMGLENRITQRFHSDTMYHAVGQGALGIEIRKGDTKMMKILDEICDLNATI 235
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISED-GILTLRAAVMNTSGTECISDEISALL---P 363
C++ER+LMR L+GGCS PI V S +E+ L L+A V++ GTE + DEI L+
Sbjct: 236 CCLSERALMRTLEGGCSVPIGVESKYNEETKKLLLKAIVVDVEGTEAVEDEIEMLIENVK 295
Query: 364 EEDIEC 381
E+ + C
Sbjct: 296 EDSMAC 301
>sp|Q8DIE4|HEM3_SYNEL Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 320
Score = 101 bits (251), Expect = 3e-21
Identities = 53/112 (47%), Positives = 72/112 (64%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
LILA AGL R+G RISQVL SLYAVGQGAL ECR++D + L L+ ++ E+
Sbjct: 173 LILAVAGLRRLGFGDRISQVLPATVSLYAVGQGALGIECRAEDADILALVKTLEHPETTA 232
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDEIS 351
C+AER+ +RQL+GGC PI V + + E+G LTL V + G + D ++
Sbjct: 233 RCLAERAFLRQLEGGCQVPIGVHT-VIENGQLTLTGLVASLDGQRLVKDSLT 283
>sp|P73660|HEM3_SYNY3 Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 320
Score = 100 bits (250), Expect = 3e-21
Identities = 56/126 (44%), Positives = 76/126 (60%)
Frame = +1
Query: 1 NSARGLILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHD 180
N +ILA AGLER+ + +RI Q++ E SL+AVGQGAL ECR D L LL + D
Sbjct: 169 NEYDAIILAAAGLERLDMANRIDQLIPPEISLHAVGQGALGIECREGDQEILSLLKVLED 228
Query: 181 DESGITCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDEISALL 360
+ S C+AER+ +RQL+GGC PI V +++ D LTL V + G I D +SA
Sbjct: 229 ENSRDRCLAERAFLRQLEGGCQVPIGVNTHLDGDN-LTLTGMVASLDGQRLIKDTLSA-- 285
Query: 361 PEEDIE 378
P ++ E
Sbjct: 286 PRKEAE 291
>sp|P08397|HEM3_HUMAN Porphobilinogen deaminase (Hydroxymethylbilane synthase) (HMBS)
(Pre-uroporphyrinogen synthase) (PBG-D)
Length = 361
Score = 99.8 bits (247), Expect = 7e-21
Identities = 70/210 (33%), Positives = 103/210 (49%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
+ILA AGL+R+G +R+ Q+L E +YAVGQGAL E R+ D + L L+ +HD E+ +
Sbjct: 186 IILATAGLQRMGWHNRVGQILHPEECMYAVGQGALGVEVRAKDQDILDLVGVLHDPETLL 245
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDEISALLPEEDI 375
CIAER+ +R L+GGCS P+AV + + +DG L L V + G++ I + + A +
Sbjct: 246 RCIAERAFLRHLEGGCSVPVAVHTAM-KDGQLYLTGGVWSLDGSDSIQETMQATIHVP-- 302
Query: 376 ECFLERIQSSDNEENDEYPPKKKLKRQNLLTACPITDVDIQLGKDANAVFLGAQVFPICE 555
Q D E+D +G A + G Q+
Sbjct: 303 ------AQHEDGPEDDPQ----------------------LVGITARNIPRGPQL----- 329
Query: 556 IGRMRMAISQRVGAELAHRLLEAGAKRILD 645
+Q +G LA+ LL GAK ILD
Sbjct: 330 -------AAQNLGISLANLLLSKGAKNILD 352
>sp|Q8YVU6|HEM3_ANASP Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 323
Score = 99.4 bits (246), Expect = 1e-20
Identities = 50/113 (44%), Positives = 72/113 (63%)
Frame = +1
Query: 13 GLILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESG 192
GLILA AGL+R+G+ R+ Q+L E SL+AVGQGAL ECR+DD + +L +I E+
Sbjct: 175 GLILAAAGLQRLGMGDRVHQILPKEISLHAVGQGALGIECRADDAELITVLKAIEHPETR 234
Query: 193 ITCIAERSLMRQLDGGCSTPIAVRSNISEDGILTLRAAVMNTSGTECISDEIS 351
C+AERS +R L+GGC PI V + I+ + ++ L V + G + D +S
Sbjct: 235 DRCLAERSFLRSLEGGCQVPIGVNTEINGNELI-LTGVVASVDGQNLVKDTVS 286
>sp|Q6FNR4|HEM3_CANGA Porphobilinogen deaminase (PBG) (Hydroxymethylbilane synthase)
(HMBS) (Pre-uroporphyrinogen synthase)
Length = 329
Score = 99.0 bits (245), Expect = 1e-20
Identities = 58/126 (46%), Positives = 77/126 (61%), Gaps = 4/126 (3%)
Frame = +1
Query: 16 LILAKAGLERIGLESRISQVLSYEGSLYAVGQGALACECRSDDFNTLKLLSSIHDDESGI 195
L+LA AGL R+GLE RI+Q L + +AVGQGAL E R D L++L I D ES +
Sbjct: 176 LVLASAGLVRMGLEDRITQRLHSDIMYHAVGQGALGIEIRQGDKKILQILDEIADLESTV 235
Query: 196 TCIAERSLMRQLDGGCSTPIAVRSNIS-EDGILTLRAAVMNTSGTECISDEISAL---LP 363
C+AERSLMR L+GGCS PI V S+ + L L+ V+N GT I D+ + +
Sbjct: 236 CCLAERSLMRTLEGGCSVPIGVESSYDHKTKKLLLKGIVVNVEGTMAIEDQQEVVVNDIR 295
Query: 364 EEDIEC 381
E+ I+C
Sbjct: 296 EDSIKC 301
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,316,807
Number of Sequences: 369166
Number of extensions: 1508970
Number of successful extensions: 5151
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4939
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5113
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7018522000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)