Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02158
(532 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinas... 124 1e-28
sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinas... 122 5e-28
sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (... 122 7e-28
sp|Q9HFW2|CLA4_ASHGO Serine/threonine-protein kinase CLA4 119 3e-27
sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20 118 8e-27
sp|P48562|CLA4_YEAST Serine/threonine-protein kinase CLA4 117 1e-26
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 116 4e-26
sp|O94804|STK10_HUMAN Serine/threonine-protein kinase 10 (L... 115 5e-26
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 115 5e-26
sp|P53351|PLK2_MOUSE Serine/threonine-protein kinase PLK2 (... 115 9e-26
>sp|Q99683|M3K5_HUMAN Mitogen-activated protein kinase kinase kinase 5 (MAPK/ERK kinase
kinase 5) (MEK kinase 5) (MEKK 5) (Apoptosis
signal-regulating kinase 1) (ASK-1)
Length = 1374
Score = 124 bits (311), Expect = 1e-28
Identities = 73/158 (46%), Positives = 97/158 (61%), Gaps = 5/158 (3%)
Frame = +2
Query: 11 YTKQILEGLKFLHNKKIIHRDIKSQNILISK-ENIIKIADFGLSRILKSISLHKKRTFCG 187
YTKQILEGLK+LH+ +I+HRDIK N+LI+ ++KI+DFG S+ L I+ TF G
Sbjct: 783 YTKQILEGLKYLHDNQIVHRDIKGDNVLINTYSGVLKISDFGTSKRLAGIN-PCTETFTG 841
Query: 188 TPNYLAPEIINGEP--YDWSADIWSLGATVFEMATGHPPYHD-SEAVTVIFKIGNTK-KI 355
T Y+APEII+ P Y +ADIWSLG T+ EMATG PP+++ E +FK+G K
Sbjct: 842 TLQYMAPEIIDKGPRGYGKAADIWSLGCTIIEMATGKPPFYELGEPQAAMFKVGMFKVHP 901
Query: 356 EYPSELVAEIIRFLDFTTTADPRNRCSAESLICCDFFK 469
E P + AE F+ DP R A L+ +F K
Sbjct: 902 EIPESMSAEAKAFILKCFEPDPDKRACANDLLVDEFLK 939
>sp|O95382|M3K6_HUMAN Mitogen-activated protein kinase kinase kinase 6
Length = 1011
Score = 122 bits (306), Expect = 5e-28
Identities = 74/155 (47%), Positives = 97/155 (62%), Gaps = 5/155 (3%)
Frame = +2
Query: 2 IKQYTKQILEGLKFLHNKKIIHRDIKSQNILISK-ENIIKIADFGLSRILKSISLHKKRT 178
I YT+QIL+GL +LH+ I+HRDIK N+LI+ ++KI+DFG S+ L I+ T
Sbjct: 471 ISFYTRQILQGLGYLHDNHIVHRDIKGDNVLINTFSGLLKISDFGTSKRLAGIT-PCTET 529
Query: 179 FCGTPNYLAPEIINGEP--YDWSADIWSLGATVFEMATGHPPYHD-SEAVTVIFKIGNTK 349
F GT Y+APEII+ P Y +ADIWSLG TV EMATG PP+H+ +F++G K
Sbjct: 530 FTGTLQYMAPEIIDQGPRGYGKAADIWSLGCTVIEMATGRPPFHELGSPQAAMFQVGMYK 589
Query: 350 -KIEYPSELVAEIIRFLDFTTTADPRNRCSAESLI 451
PS L AE FL T DPR R SA++L+
Sbjct: 590 VHPPMPSSLSAEAQAFLLRTFEPDPRLRASAQTLL 624
>sp|Q12469|SKM1_YEAST Serine/threonine-protein kinase SKM1 (Protein kinase 75490 D)
Length = 655
Score = 122 bits (305), Expect = 7e-28
Identities = 61/151 (40%), Positives = 90/151 (59%), Gaps = 2/151 (1%)
Frame = +2
Query: 17 KQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTFCGTPN 196
K+ +GLKFLHNKKIIHRDIKS NIL++ + ++KI DFG L K+ T GTP
Sbjct: 489 KETCQGLKFLHNKKIIHRDIKSDNILLNSQGLVKITDFGFCVELTE-KRSKRATMVGTPY 547
Query: 197 YLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKIGN--TKKIEYPSE 370
++APEI+N + YD D+WSLG + EM G PPY + + + ++ I N + K+ +P
Sbjct: 548 WMAPEIVNQKGYDEKVDVWSLGIMLIEMIEGEPPYLNEDPLKALYLIANNGSPKLRHPES 607
Query: 371 LVAEIIRFLDFTTTADPRNRCSAESLICCDF 463
+ + +FLD + +R S L+ +F
Sbjct: 608 VSKQTKQFLDACLQVNVESRASVRKLLTFEF 638
>sp|Q9HFW2|CLA4_ASHGO Serine/threonine-protein kinase CLA4
Length = 793
Score = 119 bits (299), Expect = 3e-27
Identities = 64/158 (40%), Positives = 86/158 (54%), Gaps = 2/158 (1%)
Frame = +2
Query: 2 IKQYTKQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTF 181
I ++ +GLKFLH+K IIHRDIKS N+L+ +KI DFG L K+ T
Sbjct: 621 IAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTHGRVKITDFGFCAKLTD-KRSKRATM 679
Query: 182 CGTPNYLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKI--GNTKKI 355
GTP ++APE++ YD D+WSLG EM G PPY + E + ++ I T K+
Sbjct: 680 VGTPYWMAPEVVKQREYDEKVDVWSLGIMTIEMLEGEPPYLNEEPLKALYLIATNGTPKL 739
Query: 356 EYPSELVAEIIRFLDFTTTADPRNRCSAESLICCDFFK 469
++P L EI RFL D R R S E L+ FF+
Sbjct: 740 KHPELLSLEIKRFLSVCLCVDVRYRASTEELLHHSFFE 777
>sp|Q03497|STE20_YEAST Serine/threonine-protein kinase STE20
Length = 939
Score = 118 bits (296), Expect = 8e-27
Identities = 58/147 (39%), Positives = 90/147 (61%), Gaps = 2/147 (1%)
Frame = +2
Query: 17 KQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTFCGTPN 196
++ L GL+FLH+K ++HRDIKS NIL+S E IK+ DFG + ++L K+ T GTP
Sbjct: 721 RETLSGLEFLHSKGVLHRDIKSDNILLSMEGDIKLTDFGFCAQINELNL-KRTTMVGTPY 779
Query: 197 YLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKI--GNTKKIEYPSE 370
++APE+++ + Y DIWSLG + EM G PPY + + ++ I T K++ P
Sbjct: 780 WMAPEVVSRKEYGPKVDIWSLGIMIIEMIEGEPPYLNETPLRALYLIATNGTPKLKEPEN 839
Query: 371 LVAEIIRFLDFTTTADPRNRCSAESLI 451
L + + +FLD+ +P +R SA L+
Sbjct: 840 LSSSLKKFLDWCLCVEPEDRASATELL 866
>sp|P48562|CLA4_YEAST Serine/threonine-protein kinase CLA4
Length = 842
Score = 117 bits (294), Expect = 1e-26
Identities = 63/157 (40%), Positives = 85/157 (54%), Gaps = 2/157 (1%)
Frame = +2
Query: 2 IKQYTKQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTF 181
I ++ +GLKFLH+K IIHRDIKS N+L+ +KI DFG L K+ T
Sbjct: 670 IAYIVRETCQGLKFLHDKHIIHRDIKSDNVLLDTRARVKITDFGFCARLTD-KRSKRATM 728
Query: 182 CGTPNYLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKI--GNTKKI 355
GTP ++APE++ YD D+WSLG EM G PPY + + + ++ I T K+
Sbjct: 729 VGTPYWMAPEVVKQREYDEKIDVWSLGIMTIEMLEGEPPYLNEDPLKALYLIATNGTPKL 788
Query: 356 EYPSELVAEIIRFLDFTTTADPRNRCSAESLICCDFF 466
++P L EI RFL D R R S E L+ FF
Sbjct: 789 KHPESLSLEIKRFLSVCLCVDVRYRASTEELLHHGFF 825
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 116 bits (290), Expect = 4e-26
Identities = 59/145 (40%), Positives = 86/145 (59%)
Frame = +2
Query: 17 KQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTFCGTPN 196
K+IL+GL +LH++K IHRDIK+ N+L+S++ +K+ADFG++ L + K+ TF GTP
Sbjct: 126 KEILKGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQI-KRNTFVGTPF 184
Query: 197 YLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKIGNTKKIEYPSELV 376
++APE+I YD ADIWSLG T E+A G PP D + V+F I +
Sbjct: 185 WMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPKNNPPTLVGDFT 244
Query: 377 AEIIRFLDFTTTADPRNRCSAESLI 451
F+D DP R +A+ L+
Sbjct: 245 KSFKEFIDACLNKDPSFRPTAKELL 269
>sp|O94804|STK10_HUMAN Serine/threonine-protein kinase 10 (Lymphocyte-oriented kinase)
Length = 968
Score = 115 bits (289), Expect = 5e-26
Identities = 66/158 (41%), Positives = 98/158 (62%), Gaps = 8/158 (5%)
Frame = +2
Query: 2 IKQYTKQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLS-RILKSISLHKKRT 178
I+ +Q+LE L FLH+K+IIHRD+K+ N+L++ E I++ADFG+S + LK +L K+ +
Sbjct: 134 IQVVCRQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLK--TLQKRDS 191
Query: 179 FCGTPNYLAPEIINGE-----PYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKIGN 343
F GTP ++APE++ E PYD+ ADIWSLG T+ EMA PP+H+ + V+ KI
Sbjct: 192 FIGTPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAK 251
Query: 344 T--KKIEYPSELVAEIIRFLDFTTTADPRNRCSAESLI 451
+ + PS+ E FL +P R SA L+
Sbjct: 252 SDPPTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLL 289
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 115 bits (289), Expect = 5e-26
Identities = 59/145 (40%), Positives = 86/145 (59%)
Frame = +2
Query: 17 KQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTFCGTPN 196
K+IL+GL +LH++K IHRDIK+ N+L+S++ +K+ADFG++ L + K+ TF GTP
Sbjct: 126 KEILKGLDYLHSEKKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQI-KRNTFVGTPF 184
Query: 197 YLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKIGNTKKIEYPSELV 376
++APE+I YD ADIWSLG T E+A G PP D + V+F I +
Sbjct: 185 WMAPEVIQQSAYDSKADIWSLGITAIELAKGEPPNSDMHPMRVLFLIPKNNPPTLIGDFT 244
Query: 377 AEIIRFLDFTTTADPRNRCSAESLI 451
F+D DP R +A+ L+
Sbjct: 245 KSFKEFIDACLNKDPSFRPTAKELL 269
>sp|P53351|PLK2_MOUSE Serine/threonine-protein kinase PLK2 (Polo-like kinase 1) (PLK-2)
(Serine/threonine-protein kinase SNK) (Serum inducible
kinase)
Length = 682
Score = 115 bits (287), Expect = 9e-26
Identities = 54/155 (34%), Positives = 95/155 (61%)
Frame = +2
Query: 2 IKQYTKQILEGLKFLHNKKIIHRDIKSQNILISKENIIKIADFGLSRILKSISLHKKRTF 181
++ Y +QI+ GLK+LH ++I+HRD+K N I++ +K+ DFGL+ L+ + H++RT
Sbjct: 179 VRYYLRQIVSGLKYLHEQEILHRDLKLGNFFINEAMELKVGDFGLAARLEPLE-HRRRTI 237
Query: 182 CGTPNYLAPEIINGEPYDWSADIWSLGATVFEMATGHPPYHDSEAVTVIFKIGNTKKIEY 361
CGTPNYL+PE++N + + +DIW+LG ++ M G PP+ ++ + ++ +
Sbjct: 238 CGTPNYLSPEVLNKQGHGCESDIWALGCVMYTMLLGRPPF-ETTNLKETYRCIREARYTM 296
Query: 362 PSELVAEIIRFLDFTTTADPRNRCSAESLICCDFF 466
PS L+A + + +P +R S + +I DFF
Sbjct: 297 PSSLLAPAKHLIASMLSKNPEDRPSLDDIIRHDFF 331
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,887,932
Number of Sequences: 369166
Number of extensions: 1135674
Number of successful extensions: 6170
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4297
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4470
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3600891075
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)