Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02048
(454 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase st... 57 1e-08
sp|Q60700|M3K12_MOUSE Mitogen-activated protein kinase kina... 57 2e-08
sp|O43283|M3K13_HUMAN Mitogen-activated protein kinase kina... 57 2e-08
sp|Q63796|M3K12_RAT Mitogen-activated protein kinase kinase... 57 2e-08
sp|Q12852|M3K12_HUMAN Mitogen-activated protein kinase kina... 57 2e-08
sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinas... 56 4e-08
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 55 5e-08
sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11 55 5e-08
sp|Q5R8X7|M3K13_PONPY Mitogen-activated protein kinase kina... 55 5e-08
sp|Q9Y7J6|KOIA_SCHPO Putative serine/threonine-protein kina... 55 5e-08
>sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase ste8) (MAPK kinase kinase)
(MAPKKK)
Length = 659
Score = 57.4 bits (137), Expect = 1e-08
Identities = 28/82 (34%), Positives = 48/82 (58%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
IKIS F +SK L +++ + G S G+ +MAPEV+ T K+D+WS G +++E
Sbjct: 536 IKISDFGISKKLELNSTSTKTGGARPSFQGSSFWMAPEVVKQTMHTEKTDIWSLGCLVIE 595
Query: 190 LVTGKQPFYGLESINIILKIAD 255
++T K P+ + + I +I +
Sbjct: 596 MLTSKHPYPNCDQMQAIFRIGE 617
>sp|Q60700|M3K12_MOUSE Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 888
Score = 56.6 bits (135), Expect = 2e-08
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
+KIS F SK L+ ST MS GT +MAPEV+ ++ ++ K D+WSFG VL E
Sbjct: 283 VKISDFGTSKELSD-KSTKMSFA------GTVAWMAPEVIRNEPVSEKVDIWSFGVVLWE 335
Query: 190 LVTGKQPFYGLESINII 240
L+TG+ P+ ++S II
Sbjct: 336 LLTGEIPYKDVDSSAII 352
>sp|O43283|M3K13_HUMAN Mitogen-activated protein kinase kinase kinase 13 (Mixed lineage
kinase) (MLK) (Leucine zipper-bearing kinase)
Length = 966
Score = 56.6 bits (135), Expect = 2e-08
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
+KIS F SK L+ ST MS GT +MAPEV+ ++ ++ K D+WSFG VL E
Sbjct: 293 VKISDFGTSKELSD-KSTKMSFA------GTVAWMAPEVIRNEPVSEKVDIWSFGVVLWE 345
Query: 190 LVTGKQPFYGLESINII 240
L+TG+ P+ ++S II
Sbjct: 346 LLTGEIPYKDVDSSAII 362
>sp|Q63796|M3K12_RAT Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 888
Score = 56.6 bits (135), Expect = 2e-08
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
+KIS F SK L+ ST MS GT +MAPEV+ ++ ++ K D+WSFG VL E
Sbjct: 283 VKISDFGTSKELSD-KSTKMSFA------GTVAWMAPEVIRNEPVSEKVDIWSFGVVLWE 335
Query: 190 LVTGKQPFYGLESINII 240
L+TG+ P+ ++S II
Sbjct: 336 LLTGEIPYKDVDSSAII 352
>sp|Q12852|M3K12_HUMAN Mitogen-activated protein kinase kinase kinase 12 (Mixed lineage
kinase) (Leucine-zipper protein kinase) (ZPK) (Dual
leucine zipper bearing kinase) (DLK) (MAPK-upstream
kinase) (MUK)
Length = 859
Score = 56.6 bits (135), Expect = 2e-08
Identities = 34/77 (44%), Positives = 48/77 (62%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
+KIS F SK L+ ST MS GT +MAPEV+ ++ ++ K D+WSFG VL E
Sbjct: 250 VKISDFGTSKELSD-KSTKMSFA------GTVAWMAPEVIRNEPVSEKVDIWSFGVVLWE 302
Query: 190 LVTGKQPFYGLESINII 240
L+TG+ P+ ++S II
Sbjct: 303 LLTGEIPYKDVDSSAII 319
>sp|Q9Y6R4|M3K4_HUMAN Mitogen-activated protein kinase kinase kinase 4 (MAPK/ERK kinase
kinase 4) (MEK kinase 4) (MEKK 4) (MAP three kinase 1)
Length = 1607
Score = 55.8 bits (133), Expect = 4e-08
Identities = 43/130 (33%), Positives = 67/130 (51%), Gaps = 9/130 (6%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKIT---RKSDVWSFGAV 180
IK+ F S L T+ E + S GT +MAPEV+ K R +D+WS G V
Sbjct: 1476 IKLGDFGCSVKLKNNAQTMPGE--VNSTLGTAAYMAPEVITRAKGEGHGRAADIWSLGCV 1533
Query: 181 LVELVTGKQPFYGLE-SINIILKIADEH-----QLIMCQEKDV*SLWIYC*MLLEAKDRW 342
++E+VTGK+P++ E + I+ K+ H + + + KD S +C + + K RW
Sbjct: 1534 VIEMVTGKRPWHEYEHNFQIMYKVGMGHKPPIPERLSPEGKDFLS---HC-LESDPKMRW 1589
Query: 343 MLIKLLEHDF 372
+LL+H F
Sbjct: 1590 TASQLLDHSF 1599
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 55.5 bits (132), Expect = 5e-08
Identities = 32/84 (38%), Positives = 49/84 (58%)
Frame = +1
Query: 7 DIKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLV 186
++K+ F SK L + MS M+S GT +M+PEV+ + RK+DVWS G +V
Sbjct: 502 NVKLGDFGASKRL---QTICMSGTGMRSVTGTPYWMSPEVISGEGYGRKADVWSLGCTVV 558
Query: 187 ELVTGKQPFYGLESINIILKIADE 258
E++T K P+ E++ I KIA +
Sbjct: 559 EMLTEKPPWAEYEAMAAIFKIATQ 582
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
Length = 738
Score = 55.5 bits (132), Expect = 5e-08
Identities = 29/80 (36%), Positives = 44/80 (55%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
+KI+ F +SK L+ N +Q G+ +M+PEV+ T K+D+WS G V++E
Sbjct: 614 VKITDFGISKKLSPLNKKQNKRASLQ---GSVFWMSPEVVKQTATTAKADIWSTGCVVIE 670
Query: 190 LVTGKQPFYGLESINIILKI 249
+ TGK PF + I KI
Sbjct: 671 MFTGKHPFPDFSQMQAIFKI 690
>sp|Q5R8X7|M3K13_PONPY Mitogen-activated protein kinase kinase kinase 13
Length = 966
Score = 55.5 bits (132), Expect = 5e-08
Identities = 33/77 (42%), Positives = 48/77 (62%)
Frame = +1
Query: 10 IKISGFSLSKYLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVWSFGAVLVE 189
+KIS F SK L+ ST MS GT ++APEV+ ++ ++ K D+WSFG VL E
Sbjct: 293 VKISDFGTSKELSD-KSTKMSFA------GTVAWLAPEVIRNEPVSEKVDIWSFGVVLWE 345
Query: 190 LVTGKQPFYGLESINII 240
L+TG+ P+ ++S II
Sbjct: 346 LLTGEIPYKDVDSSAII 362
>sp|Q9Y7J6|KOIA_SCHPO Putative serine/threonine-protein kinase C1778.10c
Length = 550
Score = 55.5 bits (132), Expect = 5e-08
Identities = 28/88 (31%), Positives = 48/88 (54%), Gaps = 5/88 (5%)
Frame = +1
Query: 1 NLDIKISGFSLSK-----YLAGANSTVMSEGFMQSKPGTCNFMAPEVLIDQKITRKSDVW 165
N+ +KI+ F +K Y+ + T F+ GT ++APE+L Q +++ SD+W
Sbjct: 189 NMHVKITDFGTAKILPPKYVNSPDYTTFPSSFV----GTAEYVAPELLSRQVVSKSSDLW 244
Query: 166 SFGAVLVELVTGKQPFYGLESINIILKI 249
+F V+ +++ G PF+G NI KI
Sbjct: 245 AFACVVYQMIVGSPPFHGSNPNNIFKKI 272
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 54,793,002
Number of Sequences: 369166
Number of extensions: 1082526
Number of successful extensions: 3940
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3885
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2435140505
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)