Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02027
(766 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX) 116 6e-26
sp|P52032|GPX1_ARATH Phospholipid hydroperoxide glutathione... 107 5e-23
sp|P74250|GPO_SYNY3 Putative glutathione peroxidase 107 5e-23
sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione p... 107 5e-23
sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione... 105 1e-22
sp|O24296|GPX1_PEA Phospholipid hydroperoxide glutathione p... 105 1e-22
sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione... 105 2e-22
sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione... 104 2e-22
sp|Q4AEH0|GPX4_MACFU Phospholipid hydroperoxide glutathione... 103 7e-22
sp|O23970|GPX1_HELAN Glutathione peroxidase 1 101 3e-21
>sp|Q00277|GPX1_SCHMA Glutathione peroxidase (GPX)
Length = 169
Score = 116 bits (291), Expect = 6e-26
Identities = 61/161 (37%), Positives = 95/161 (59%), Gaps = 4/161 (2%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
++Y+F+ DI+G V+ EKYR V LIVN ACK +T +Y Q+Q + + +G R+LA
Sbjct: 10 SIYEFTVKDINGVDVSLEKYRGHVCLIVNVACKCGATDKNYRQLQEMHTRLVGKGLRILA 69
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLER----STN 555
F +QFG ++ +++ +IK FV E F+MF + + G A L+KFL+ +
Sbjct: 70 FPCNQFGGQE--PWAEAEIKKFVTEKYGVQFDMFSKIKVNGSDADDLYKFLKSRQHGTLT 127
Query: 556 SHIIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLLNQ 678
++I WNF+KFLV + G P K Y P+D+ +I LL +
Sbjct: 128 NNIKWNFSKFLVDRQGQPVKRYSPTTAPYDIEGDIMELLEK 168
>sp|P52032|GPX1_ARATH Phospholipid hydroperoxide glutathione peroxidase, chloroplast
precursor (PHGPx)
Length = 236
Score = 107 bits (266), Expect = 5e-23
Identities = 59/159 (37%), Positives = 90/159 (56%), Gaps = 4/159 (2%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
TV+ F+ DIDG V K++ +V+LIVN A + T S+Y+++ L KY +GF +LA
Sbjct: 78 TVHDFTVKDIDGKDVALNKFKGKVMLIVNVASRCGLTSSNYSELSHLYEKYKTQGFEILA 137
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERSTNSH-- 561
F +QFG ++ S+ +IK F A F +F VD+ GPS P+++FL+ +
Sbjct: 138 FPCNQFGFQEPG--SNSEIKQFACTRFKAEFPIFDKVDVNGPSTAPIYEFLKSNAGGFLG 195
Query: 562 --IIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLL 672
I WNF KFL+ K G + Y PF + ++I+ LL
Sbjct: 196 GLIKWNFEKFLIDKKGKVVERYPPTTSPFQIEKDIQKLL 234
>sp|P74250|GPO_SYNY3 Putative glutathione peroxidase
Length = 169
Score = 107 bits (266), Expect = 5e-23
Identities = 64/165 (38%), Positives = 90/165 (54%), Gaps = 10/165 (6%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
T+Y FSA +DG+ V + +VLLIVNTA + T Y +Q L ++ RGF VL
Sbjct: 8 TIYGFSANALDGSPVALRDFEGKVLLIVNTASQCGFT-PQYQGLQALYNRFGDRGFTVLG 66
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERSTNSHII 567
F +QFG+++ +IKNF E TF +F V++ GP+AHPLFKFL ++ I
Sbjct: 67 FPCNQFGQQEPG--GSGEIKNFCETRYGVTFPLFEKVEVNGPNAHPLFKFLTAASPGMAI 124
Query: 568 ----------WNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLL 672
WNF KFLV + G K Y + +P ++ +IE LL
Sbjct: 125 PFLGGAEDIKWNFTKFLVDRQGKVVKRYGSIAKPDEIAADIEKLL 169
>sp|P36968|GPX4_PIG Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 107 bits (266), Expect = 5e-23
Identities = 61/161 (37%), Positives = 90/161 (55%), Gaps = 6/161 (3%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
++++FSA DIDG+ VN +KYR V ++ N A + T +Y Q+ L A+YA G R+LA
Sbjct: 40 SMHEFSAKDIDGHMVNLDKYRGYVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILA 99
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERSTNSH-- 561
F +QFGR++ SD +IK F N F+MF + + G AHPL+K+++
Sbjct: 100 FPCNQFGRQEPG--SDAEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKVQPKGRGM 156
Query: 562 ----IIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLL 672
I WNF KFL+ KNG K Y EP + +++ L
Sbjct: 157 LGNAIKWNFTKFLIDKNGCVVKRYGPMEEPQVIEKDLPCYL 197
>sp|Q4AEG9|GPX4_CEBAP Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 105 bits (263), Expect = 1e-22
Identities = 60/151 (39%), Positives = 87/151 (57%), Gaps = 6/151 (3%)
Frame = +1
Query: 205 GTVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVL 384
G++++FSA DIDG+ VN +KYR V ++ N A + T +Y Q+ L A+YA G R+L
Sbjct: 39 GSMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRIL 98
Query: 385 AFANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERS----- 549
AF +QFG K+ S+++IK F N F+MF + + G AHPL+K+++
Sbjct: 99 AFPCNQFG--KQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKG 155
Query: 550 -TNSHIIWNFNKFLVGKNGVPYKFYDNKIEP 639
+ I WNF KFL+ KNG K Y EP
Sbjct: 156 ILGNAIKWNFTKFLIDKNGCVVKRYGPMEEP 186
>sp|O24296|GPX1_PEA Phospholipid hydroperoxide glutathione peroxidase, chloroplast
precursor (PHGPx)
Length = 236
Score = 105 bits (262), Expect = 1e-22
Identities = 58/159 (36%), Positives = 90/159 (56%), Gaps = 4/159 (2%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
T+Y F+ DID V+ K++ +VLLIVN A + T S+Y ++ L + ++G VLA
Sbjct: 78 TIYDFTVKDIDKKDVSLSKFKGKVLLIVNVASRCGLTSSNYTELSHLYENFKNKGLEVLA 137
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERSTNSH-- 561
F +QFG ++ S+++IK F A F +F VD+ GP P+++FL+ S+
Sbjct: 138 FPCNQFGMQEPG--SNEEIKQFACTKFKAEFPIFDKVDVNGPFTAPVYQFLKSSSGGFFG 195
Query: 562 --IIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLL 672
+ WNF KFLV KNG + Y PF + ++I+ LL
Sbjct: 196 DIVKWNFEKFLVDKNGKVVERYPPTTSPFQIEKDIQKLL 234
>sp|Q9N2J2|GPX4_BOVIN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 105 bits (261), Expect = 2e-22
Identities = 59/161 (36%), Positives = 89/161 (55%), Gaps = 6/161 (3%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
++++FSA DIDG VN +KYR V ++ N A + T +Y Q+ L A+YA G R+LA
Sbjct: 40 SMHEFSAKDIDGRMVNLDKYRGHVCIVTNVASQCGKTDVNYTQLVDLHARYAECGLRILA 99
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERSTNSH-- 561
F +QFGR++ S+ +IK F N F++F + + G AHPL+K+++
Sbjct: 100 FPCNQFGRQEPG--SNAEIKEFA-AGYNVKFDLFSKICVNGDDAHPLWKWMKVQPKGRGM 156
Query: 562 ----IIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLL 672
I WNF KFL+ KNG K Y EP + +++ L
Sbjct: 157 LGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDLPCYL 197
>sp|Q4AEH2|GPX4_PONPY Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
sp|P36969|GPX4_HUMAN Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (GPX-4)
Length = 197
Score = 104 bits (260), Expect = 2e-22
Identities = 59/157 (37%), Positives = 90/157 (57%), Gaps = 6/157 (3%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
++++FSA DIDG+ VN +KYR V ++ N A + T +Y Q+ L A+YA G R+LA
Sbjct: 40 SMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGLRILA 99
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERS------ 549
F +QFG K+ S+++IK F N F+MF + + G AHPL+K+++
Sbjct: 100 FPCNQFG--KQEPGSNEEIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGI 156
Query: 550 TNSHIIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEI 660
+ I WNF KFL+ KNG K Y EP + +++
Sbjct: 157 LGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
>sp|Q4AEH0|GPX4_MACFU Phospholipid hydroperoxide glutathione peroxidase, mitochondrial
precursor (PHGPx) (Glutathione peroxidase 4) (GPx-4)
Length = 197
Score = 103 bits (256), Expect = 7e-22
Identities = 59/157 (37%), Positives = 89/157 (56%), Gaps = 6/157 (3%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
++++FSA DIDG+ VN +KYR V ++ N A + T +Y Q+ L A+YA G R+LA
Sbjct: 40 SMHEFSAKDIDGHMVNLDKYRGFVCIVTNVASQCGKTEVNYTQLVDLHARYAECGVRILA 99
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERS------ 549
F +QFG K+ S++ IK F N F+MF + + G AHPL+K+++
Sbjct: 100 FPCNQFG--KQEPGSNEKIKEFA-AGYNVKFDMFSKICVNGDDAHPLWKWMKIQPKGKGI 156
Query: 550 TNSHIIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEI 660
+ I WNF KFL+ KNG K Y EP + +++
Sbjct: 157 LGNAIKWNFTKFLIDKNGCVVKRYGPMEEPLVIEKDL 193
>sp|O23970|GPX1_HELAN Glutathione peroxidase 1
Length = 167
Score = 101 bits (251), Expect = 3e-21
Identities = 55/159 (34%), Positives = 90/159 (56%), Gaps = 4/159 (2%)
Frame = +1
Query: 208 TVYKFSAYDIDGNYVNFEKYRNRVLLIVNTACK*KSTHSHYAQMQTLQAKYAHRGFRVLA 387
T+Y F+ D GN V+ Y+ +V+LIVN A K T++ Y ++ + KY +GF +LA
Sbjct: 8 TLYDFTVKDAKGNDVDLSVYKGKVVLIVNVASKCGLTNNSYDELNQIYLKYKEKGFEILA 67
Query: 388 FANSQFGREKETKYSDKDIKNFVEENVNATFEMFRNVDIVGPSAHPLFKFLERS----TN 555
F +QFG+++ ++++I +FV + F +F +D+ G +A P+++FL+
Sbjct: 68 FPCNQFGQQEPG--TNEEIVDFVCTKFKSEFPIFDKIDVNGENAAPVYEFLKTGFYGILG 125
Query: 556 SHIIWNFNKFLVGKNGVPYKFYDNKIEPFDLTEEIEMLL 672
I WNF+KFLV KNG P Y P + +I+ LL
Sbjct: 126 GDIQWNFSKFLVDKNGQPVDCYYPTTSPLTVERDIQKLL 164
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 73,535,080
Number of Sequences: 369166
Number of extensions: 1346979
Number of successful extensions: 3764
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3539
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3640
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 7066925600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)