Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_02009
(575 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P46461|NSF1_DROME Vesicular-fusion protein Nsf1 (N-ethyl... 61 2e-09
sp|P54351|NSF2_DROME Vesicular-fusion protein Nsf2 (N-ethyl... 60 4e-09
sp|P18708|NSF_CRIGR Vesicle-fusing ATPase (Vesicular-fusion... 60 4e-09
sp|P46460|NSF_MOUSE Vesicle-fusing ATPase (Vesicular-fusion... 59 7e-09
sp|P46459|NSF_HUMAN Vesicle-fusing ATPase (Vesicular-fusion... 58 2e-08
sp|P34732|SEC18_CANAL Vesicular-fusion protein SEC18 54 3e-07
sp|P18759|SEC18_YEAST Vesicular-fusion protein SEC18 48 2e-05
sp|Q94392|NSF_CAEEL Vesicle-fusing ATPase (Vesicular-fusion... 43 5e-04
sp|Q9P7Q4|SEC18_SCHPO Vesicular-fusion protein SEC18 homolog 41 0.002
sp|Q9M0Y8|NSF_ARATH Vesicle-fusing ATPase (Vesicular-fusion... 40 0.004
>sp|P46461|NSF1_DROME Vesicular-fusion protein Nsf1 (N-ethylmaleimide-sensitive fusion
protein 1) (NEM-sensitive fusion protein 1) (dNsf-1)
(Comatose protein)
Length = 745
Score = 60.8 bits (146), Expect = 2e-09
Identities = 36/129 (27%), Positives = 72/129 (55%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPR+S ++ L+ ++ PK +K+LI+ T++ ++E+E+ AF + L VP ++
Sbjct: 614 IGPRYSNMTLQALLVLLKKQPPKGRKLLILCTSSRREVLEEMEMLTAF-TSVLHVPNLSK 672
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLKSVNPEMRINK 362
+++VL E D+F +G+++ + K++ + IGIK+L+ + + R K
Sbjct: 673 PDHVLAVL-ENTDIFSKGEIQAIGKKM---AGKRVFIGIKKLLGLIDMARQTEQSQRAIK 728
Query: 363 LLMKLKASG 389
L K++ G
Sbjct: 729 FLSKMEEEG 737
>sp|P54351|NSF2_DROME Vesicular-fusion protein Nsf2 (N-ethylmaleimide-sensitive fusion
protein 2) (NEM-sensitive fusion protein 2) (dNsf-2)
Length = 752
Score = 60.1 bits (144), Expect = 4e-09
Identities = 36/129 (27%), Positives = 72/129 (55%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPR+S ++ L+ ++ PK +K+LI+ T++ ++E+E+ AF + L V ++
Sbjct: 619 IGPRYSNLTLQALLVLLKKQPPKGRKLLILCTSSRRDVLEEMEMLSAF-TSVLHVSNLST 677
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLKSVNPEMRINK 362
+++VL + DLF +L+ + +++ L IGIK+L+ +++ P R+ K
Sbjct: 678 PEHVLAVL-DDSDLFSPEELQSIARKM---AGKRLCIGIKKLLALIDMIRQSEPHQRVIK 733
Query: 363 LLMKLKASG 389
L K++ G
Sbjct: 734 FLSKMEEEG 742
>sp|P18708|NSF_CRIGR Vesicle-fusing ATPase (Vesicular-fusion protein NSF)
(N-ethylmaleimide sensitive fusion protein)
(NEM-sensitive fusion protein)
Length = 744
Score = 60.1 bits (144), Expect = 4e-09
Identities = 38/129 (29%), Positives = 72/129 (55%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS +++ L+ ++ P+ +K+LIIGTT+ ++E+E+ AF + VP I
Sbjct: 614 IGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAF-STTIHVPNIAT 672
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLKSVNPEMRINK 362
+++ L E F++ + + +++K + IGIK+L+ ++ ++PE R+ K
Sbjct: 673 GEQLLEAL-ELLGNFKDKERTTIAQQVK---GKKVWIGIKKLLMLIEMSLQMDPEYRVRK 728
Query: 363 LLMKLKASG 389
L L+ G
Sbjct: 729 FLALLREEG 737
>sp|P46460|NSF_MOUSE Vesicle-fusing ATPase (Vesicular-fusion protein NSF)
(N-ethylmaleimide sensitive fusion protein)
(NEM-sensitive fusion protein) (SKD2 protein)
Length = 744
Score = 59.3 bits (142), Expect = 7e-09
Identities = 37/129 (28%), Positives = 72/129 (55%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS +++ L+ ++ P+ +K+LIIGTT+ ++E+E+ AF + VP I
Sbjct: 614 IGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAF-STTIHVPNIAT 672
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLKSVNPEMRINK 362
+++ L E F++ + + +++K + IGIK+L+ ++ ++PE R+ K
Sbjct: 673 GEQLLEAL-ELLGNFKDKERTTIAQQVK---GKKVWIGIKKLLMLIEMSLQMDPEYRVRK 728
Query: 363 LLMKLKASG 389
L ++ G
Sbjct: 729 FLALMREEG 737
>sp|P46459|NSF_HUMAN Vesicle-fusing ATPase (Vesicular-fusion protein NSF)
(N-ethylmaleimide sensitive fusion protein)
(NEM-sensitive fusion protein)
Length = 744
Score = 57.8 bits (138), Expect = 2e-08
Identities = 37/129 (28%), Positives = 71/129 (55%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS +++ L+ ++ P+ +K+LIIGTT+ ++E+E+ AF + VP I
Sbjct: 614 IGPRFSNLVLQALLVLLKKAPPQGRKLLIIGTTSRKDVLQEMEMLNAF-STTIHVPNIAT 672
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLKSVNPEMRINK 362
+++ L E ++ + + +++K + IGIK+L+ ++ ++PE R+ K
Sbjct: 673 GEQLLEAL-ELLGNLKDKERTTIAQQVK---GKKVWIGIKKLLMLIEMSLQMDPEYRVRK 728
Query: 363 LLMKLKASG 389
L L+ G
Sbjct: 729 FLALLREEG 737
>sp|P34732|SEC18_CANAL Vesicular-fusion protein SEC18
Length = 794
Score = 53.9 bits (128), Expect = 3e-07
Identities = 34/110 (30%), Positives = 60/110 (54%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS ++++ L+ + PK +++LIIGTT+ K + + +F +A+ VP I H
Sbjct: 671 IGPRFSNDILQVLMVYLTKKPPKGRRLLIIGTTSQYQVFKHMNLIDSF-NDAIAVPPIKH 729
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLK 332
E+ VL ++ + + E + +L +++IGIK L+ V K
Sbjct: 730 IEEVGKVL-DKLGFMNKSEREEILSQLS---RYDINIGIKSLIDVLMVSK 775
>sp|P18759|SEC18_YEAST Vesicular-fusion protein SEC18
Length = 758
Score = 48.1 bits (113), Expect = 2e-05
Identities = 31/122 (25%), Positives = 69/122 (56%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS N+++ L + P+++++LI+ TT+ + +++++I F N + VP +T+
Sbjct: 635 IGPRFSNNILQMLKVALKRKPPQDRRLLIMTTTSAYSVLQQMDILSCF-DNEIAVPNMTN 693
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTNLSIGIKQLVKECKVLKSVNPEMRINK 362
E+ +V+ E L G ++ + + + N ++GIK+ + + + + E +N+
Sbjct: 694 LDELNNVMIESNFLDDAGRVKVINELSRS--CPNFNVGIKKTLTNIETAR--HDEDPVNE 749
Query: 363 LL 368
L+
Sbjct: 750 LV 751
>sp|Q94392|NSF_CAEEL Vesicle-fusing ATPase (Vesicular-fusion protein NSF)
(N-ethylmaleimide sensitive fusion protein)
(NEM-sensitive fusion protein)
Length = 824
Score = 43.1 bits (100), Expect = 5e-04
Identities = 24/124 (19%), Positives = 68/124 (54%), Gaps = 2/124 (1%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
+GPR+S +++ L+ ++ P ++ +I T++ ++++ + FG + + +P++T
Sbjct: 691 VGPRYSNLVIQALLVLLNAPPPAGHRLFVIATSSDRMFLRDMGLMDVFG-DVIDIPKLTT 749
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKELKMDLSTN--LSIGIKQLVKECKVLKSVNPEMRI 356
A ++++V+ ++ +++ + L +E++L +GIK L++ + + + R+
Sbjct: 750 AGQMMNVI-QESNIYSDDQLPMIEQKLASICRGEGFHGVGIKHLLELIESARQCEADYRV 808
Query: 357 NKLL 368
LL
Sbjct: 809 PTLL 812
>sp|Q9P7Q4|SEC18_SCHPO Vesicular-fusion protein SEC18 homolog
Length = 792
Score = 40.8 bits (94), Expect = 0.002
Identities = 24/112 (21%), Positives = 61/112 (54%), Gaps = 2/112 (1%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS L++ L+ + PK ++LI+ TT+ T + +++ +F + VP +++
Sbjct: 663 IGPRFSNTLLQTLMVLFKKQPPKGHRLLILATTSERTMLSRMDMTQSFDAE-IAVPNVSN 721
Query: 183 ASEIVSVLAEQQDLFREGDL--ERLEKELKMDLSTNLSIGIKQLVKECKVLK 332
+E+ ++ + D F + ++ + L++ + +++G+ +++ + K
Sbjct: 722 VTELDRII-QSIDSFADSNVRADTLQRLQNFTGTDAVNVGVAKILMIAETAK 772
>sp|Q9M0Y8|NSF_ARATH Vesicle-fusing ATPase (Vesicular-fusion protein NSF)
(N-ethylmaleimide sensitive fusion protein)
Length = 742
Score = 40.0 bits (92), Expect = 0.004
Identities = 28/87 (32%), Positives = 45/87 (51%)
Frame = +3
Query: 3 IGPRFSINLVKNLIGMIACNVPKNKKMLIIGTTTHLTAMKELEIFFAFGKNALRVPEITH 182
IGPRFS + + L+ ++ PK KK+L+ GTT+ +T ++ + I F VP T
Sbjct: 608 IGPRFSNIISQTLMVLLKRLPPKGKKLLVFGTTSEVTFLESVGISDCFSVTH-SVP--TL 664
Query: 183 ASEIVSVLAEQQDLFREGDLERLEKEL 263
E + Q +LF E D++ + L
Sbjct: 665 QKEDAKKVLNQLNLFSEDDVDSAAEAL 691
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,306,181
Number of Sequences: 369166
Number of extensions: 1000714
Number of successful extensions: 2544
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2475
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2541
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4275400980
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)