Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= DrC_01896 (954 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|Q8UVX0|PIWI_BRARE Piwi protein 140 4e-33 sp|Q9VKM1|PIWI_DROME Piwi protein 131 3e-30 sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein 103 7e-22 sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 94 4e-19 sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation fact... 92 3e-18 sp|Q9H9G7|I2C3_HUMAN Eukaryotic translation initiation fact... 91 4e-18 sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation fact... 89 1e-17 sp|Q9UL18|I2C1_HUMAN Eukaryotic translation initiation fact... 88 3e-17 sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor... 86 2e-16 sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation fact... 86 2e-16
>sp|Q8UVX0|PIWI_BRARE Piwi protein Length = 858 Score = 140 bits (354), Expect = 4e-33 Identities = 94/252 (37%), Positives = 145/252 (57%), Gaps = 9/252 (3%) Frame = +1 Query: 136 KMALVFVPDDRV--YAKVKSFT-MSTGLLTQCVTTRNGTNRNDKRRKVVSNKTVMQIFAK 306 +M +V +P +R Y VK + + +QCV +R T + V+ K +Q+ K Sbjct: 551 QMVVVILPTNRKDKYDCVKKYLCVDCPTPSQCVVSR--TISKPQALMTVATKIALQMNCK 608 Query: 307 FGYDPWTVEIKLRPTMIVGMDTYHNKSS-KKSIQASVFSINSTFTQYMS-FVNQPRGRQE 480 G + W+VEI LR MIVG+D YH+ ++ K+SI A V S+N +++ S V Q RG QE Sbjct: 609 MGGELWSVEIPLRQLMIVGIDCYHDTAAGKRSIGAMVASLNQGMSRWFSKCVLQNRG-QE 667 Query: 481 FHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKS 660 + L + AL+ + K + LP RI+V+RDGVGD LQ ++EV + I+ + + Sbjct: 668 IIDALKGSLQGALKAYLKYNNSLPSRIIVYRDGVGDGMLQSVVDYEVPQIMQSIKTMGQD 727 Query: 661 NGFAVPQIIYVIVKKRVGTKLFNRGN----NPNPGTVVDKEIVKPNFYEFFLVSQRTTKG 828 P++ V+VKKR+ ++ F R + NP PGTV+D E+ +P +Y+FF+VSQ G Sbjct: 728 YE---PKLSVVVVKKRISSRFFARIDGKIANPPPGTVIDTEVTRPEWYDFFIVSQAVRFG 784 Query: 829 TATPTNYNVLED 864 PT+YNV+ D Sbjct: 785 CVAPTHYNVVFD 796
>sp|Q9VKM1|PIWI_DROME Piwi protein Length = 843 Score = 131 bits (329), Expect = 3e-30 Identities = 88/287 (30%), Positives = 139/287 (48%), Gaps = 9/287 (3%) Frame = +1 Query: 94 RCLNDAARSGSGCAKMALVFVPDDRV--YAKVKSFTMSTGLLTQCVTTRNGTNRNDKRRK 267 R ++D RS K+ L VP+D Y+ +K G + + V T+ T + K R Sbjct: 526 RAMDDCVRSDP---KLILCLVPNDNAERYSSIKK----RGYVDRAVPTQVVTLKTTKNRS 578 Query: 268 VVS--NKTVMQIFAKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSI-----N 426 ++S K +Q+ K GY PW +E+ L M +G D + +K ++ + N Sbjct: 579 LMSIATKIAIQLNCKLGYTPWMIELPLSGLMTIGFDIAKSTRDRKRAYGALIASMDLQQN 638 Query: 427 STFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVGDNQLQFT 606 ST Y S V + TL AL ++ + LP RI+ +RDGV L+ Sbjct: 639 ST---YFSTVTECSAFDVLANTLWPMIAKALRQYQHEHRKLPSRIVFYRDGVSSGSLKQL 695 Query: 607 KNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLFNRGNNPNPGTVVDKEIVKPN 786 FEV + ++ Y + PQ+ Y++V + + T+ F G NP PGT+VD I P Sbjct: 696 FEFEVKDIIEKLKTEYARVQLSPPQLAYIVVTRSMNTRFFLNGQNPPPGTIVDDVITLPE 755 Query: 787 FYEFFLVSQRTTKGTATPTNYNVLEDTRLLTKKGTMDPMAPNELQKL 927 Y+F+LVSQ+ +GT +PT+YNVL + ++P ++QKL Sbjct: 756 RYDFYLVSQQVRQGTVSPTSYNVLYSSM---------GLSPEKMQKL 793
>sp|Q9VUQ5|AGO2_DROME Argonaute 2 protein Length = 1214 Score = 103 bits (257), Expect = 7e-22 Identities = 84/261 (32%), Positives = 128/261 (49%), Gaps = 18/261 (6%) Frame = +1 Query: 139 MALVFVPDDRV-YAKVKSFT-MSTGLLTQCV----TTRNGTNRNDKRRKVVSNKTVMQIF 300 +A+V +P R+ Y +K + G+LTQC+ R N+ + N + I Sbjct: 885 LAIVIIPQFRISYDTIKQKAELQHGILTQCIKQFTVERKCNNQTIGNILLKINSKLNGIN 944 Query: 301 AKFGYDPWTVEIKLRPTMIVGMDTYHNKSSKKSIQASVFSINSTFTQYMSFVN-QPRGRQ 477 K DP +K TM +G D H ++ I SV + ++ Y + N Q R ++ Sbjct: 945 HKIKDDPRLPMMK--NTMYIGADVTHPSPDQREIP-SVVGVAASHDPYGASYNMQYRLQR 1001 Query: 478 EFHETLGKNFNLALED---FKKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIEN 648 E + F++ LE +K+ + P I+ +RDGV D Q KN E+ +K + Sbjct: 1002 GALEEIEDMFSITLEHLRVYKEYRNAYPDHIIYYRDGVSDGQFPKIKNEELRCIKQACDK 1061 Query: 649 IYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNNPNPGTVVDKEIVKPNFYEFFL 804 + P+I VIV KR T+ F N+ NN +PGTVVD+ IV PN +FF+ Sbjct: 1062 VG-----CKPKICCVIVVKRHHTRFFPSGDVTTSNKFNNVDPGTVVDRTIVHPNEMQFFM 1116 Query: 805 VSQRTTKGTATPTNYNVLEDT 867 VS + +GTA PT YNV+E+T Sbjct: 1117 VSHQAIQGTAKPTRYNVIENT 1137
>sp|Q9SJK3|AGOL_ARATH Argonaute-like protein At2g27880 Length = 997 Score = 94.4 bits (233), Expect = 4e-19 Identities = 87/303 (28%), Positives = 141/303 (46%), Gaps = 38/303 (12%) Frame = +1 Query: 70 TCGPNDVERCLNDAARSGSGCAKMALVFVPD-DRVYAKVKSFTMST-GLLTQCVTTRNGT 243 +C P +E L D + G ++ +V +PD Y K+K + G+++QC R Sbjct: 616 SCPPEHIEEALLDIHKRAPGL-QLLIVILPDVTGSYGKIKRICETELGIVSQCCQPR--- 671 Query: 244 NRNDKRRKVVSNKTVMQIFAKFG------YDPWTVEIKL---RPTMIVGMDTYH---NKS 387 + +K K ++I K G D I L RPT+I+G D H + Sbjct: 672 -QVNKLNKQYMENVALKINVKTGGRNTVLNDAIRRNIPLITDRPTIIMGADVTHPQPGED 730 Query: 388 SKKSIQASVFSIN-STFTQYMSFVNQPRGRQEFHETLGK----------NFNLALEDF-- 528 S SI A V S++ +Y V+ R+E + L K + L E F Sbjct: 731 SSPSIAAVVASMDWPEINKYRGLVSAQAHREEIIQDLYKLVQDPQRGLVHSGLIREHFIA 790 Query: 529 -KKRYDILPQRILVFRDGVGDNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKK 705 ++ +PQRI+ +RDGV + Q E+ A++ ++ ++ VP++ +VIV+K Sbjct: 791 FRRATGQIPQRIIFYRDGVSEGQFSQVLLHEMTAIRKACNSLQENY---VPRVTFVIVQK 847 Query: 706 RVGTKLF----------NRGNNPNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNV 855 R T+LF ++ N PGTVVD +I PN ++F+L S +GT+ P +Y+V Sbjct: 848 RHHTRLFPEQHGNRDMTDKSGNIQPGTVVDTKICHPNEFDFYLNSHAGIQGTSRPAHYHV 907 Query: 856 LED 864 L D Sbjct: 908 LLD 910
>sp|Q8CJF9|I2C3_MOUSE Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3) (Argonaute-3) (Piwi/argonaute family protein meIF2C3) Length = 860 Score = 91.7 bits (226), Expect = 3e-18 Identities = 80/286 (27%), Positives = 128/286 (44%), Gaps = 18/286 (6%) Frame = +1 Query: 76 GPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMST-GLLTQCVTTRNGTNRN 252 G + VE + SG + ++ VYA+VK + G+ TQCV +N + Sbjct: 498 GADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTS 557 Query: 253 DKRRKVVSNKTVMQIFAKFG------YDPWTVEIKLRPTMIVGMDTYH---NKSSKKSIQ 405 + +SN ++I K G + +P + +G D H K SI Sbjct: 558 PQ---TLSN-LCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIA 613 Query: 406 ASVFSINSTFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVG 585 A V S+++ ++Y + V R RQE + L L F K P RI+ +RDGV Sbjct: 614 AVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVS 673 Query: 586 DNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNN 741 + Q + +E+ A++ ++ K P I Y++V+KR T+LF R N Sbjct: 674 EGQFRQVLYYELLAIREACISLEKDYQ---PGITYIVVQKRHHTRLFCADRTERVGRSGN 730 Query: 742 PNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLT 879 GT VD +I P ++F+L S +GT+ P++Y+VL D T Sbjct: 731 IPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNFFT 776
>sp|Q9H9G7|I2C3_HUMAN Eukaryotic translation initiation factor 2C 3 (eIF2C 3) (eIF-2C 3) (Argonaute-3) Length = 860 Score = 91.3 bits (225), Expect = 4e-18 Identities = 80/286 (27%), Positives = 128/286 (44%), Gaps = 18/286 (6%) Frame = +1 Query: 76 GPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMST-GLLTQCVTTRNGTNRN 252 G + VE + SG + ++ VYA+VK + G+ TQCV +N + Sbjct: 498 GADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTS 557 Query: 253 DKRRKVVSNKTVMQIFAKFG------YDPWTVEIKLRPTMIVGMDTYH---NKSSKKSIQ 405 + +SN ++I K G + +P + +G D H K SI Sbjct: 558 PQ---TLSN-LCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIA 613 Query: 406 ASVFSINSTFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVG 585 A V S+++ ++Y + V R RQE + L L F K P RI+ +RDGV Sbjct: 614 AVVGSMDAHPSRYCATVRVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVS 673 Query: 586 DNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNN 741 + Q + +E+ A++ ++ K P I Y++V+KR T+LF R N Sbjct: 674 EGQFRQVLYYELLAIREACISLEKDYQ---PGITYIVVQKRHHTRLFCADRTERVGRSGN 730 Query: 742 PNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLT 879 GT VD +I P ++F+L S +GT+ P++Y+VL D T Sbjct: 731 IPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLWDDNCFT 776
>sp|Q8CJG1|I2C1_MOUSE Eukaryotic translation initiation factor 2C 1 (eIF2C 1) (eIF-2C 1) (Argonaute-1) (Piwi/argonaute family protein meIF2C1) Length = 857 Score = 89.4 bits (220), Expect = 1e-17 Identities = 81/286 (28%), Positives = 126/286 (44%), Gaps = 18/286 (6%) Frame = +1 Query: 76 GPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMST-GLLTQCVTTRNGTNRN 252 G + VE + SG + ++ VYA+VK + G+ TQCV +N + Sbjct: 495 GADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNAVKTS 554 Query: 253 DKRRKVVSNKTVMQIFAKFG------YDPWTVEIKLRPTMIVGMDTYH---NKSSKKSIQ 405 + +SN ++I K G + +P + +G D H K SI Sbjct: 555 PQ---TLSN-LCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSIT 610 Query: 406 ASVFSINSTFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVG 585 A V S+++ ++Y + V R RQE E L L F K P RI+ +RDGV Sbjct: 611 AVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVP 670 Query: 586 DNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNN 741 + QL ++E+ A++ + K P I Y++V+KR T+LF + N Sbjct: 671 EGQLPQILHYELLAIRDACIKLEKD---YQPGITYIVVQKRHHTRLFCADKNERIGKSGN 727 Query: 742 PNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLT 879 GT VD I P ++F+L S +GT+ P++Y VL D T Sbjct: 728 IPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFT 773
>sp|Q9UL18|I2C1_HUMAN Eukaryotic translation initiation factor 2C 1 (eIF2C 1) (eIF-2C 1) (Argonaute-1) (Putative RNA-binding protein Q99) Length = 857 Score = 88.2 bits (217), Expect = 3e-17 Identities = 81/286 (28%), Positives = 126/286 (44%), Gaps = 18/286 (6%) Frame = +1 Query: 76 GPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMST-GLLTQCVTTRNGTNRN 252 G + VE + SG + ++ VYA+VK + G+ TQCV +N + Sbjct: 495 GADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVVKTS 554 Query: 253 DKRRKVVSNKTVMQIFAKFG------YDPWTVEIKLRPTMIVGMDTYH---NKSSKKSIQ 405 + +SN ++I K G + +P + +G D H K SI Sbjct: 555 PQ---TLSN-LCLKINVKLGGINNILVPHQRSAVFQQPVIFLGADVTHPPAGDGKKPSIT 610 Query: 406 ASVFSINSTFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVG 585 A V S+++ ++Y + V R RQE E L L F K P RI+ +RDGV Sbjct: 611 AVVGSMDAHPSRYCATVRVQRPRQEIIEDLSYMVRELLIQFYKSTRFKPTRIIFYRDGVP 670 Query: 586 DNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNN 741 + QL ++E+ A++ + K P I Y++V+KR T+LF + N Sbjct: 671 EGQLPQILHYELLAIRDACIKLEKD---YQPGITYIVVQKRHHTRLFCADKNERIGKSGN 727 Query: 742 PNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLEDTRLLT 879 GT VD I P ++F+L S +GT+ P++Y VL D T Sbjct: 728 IPAGTTVDTNITHPFEFDFYLCSHAGIQGTSRPSHYYVLWDDNRFT 773
>sp|Q9QZ81|I2C2_RAT Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Golgi ER protein 95 kDa) (GERp95) Length = 860 Score = 85.9 bits (211), Expect = 2e-16 Identities = 77/281 (27%), Positives = 122/281 (43%), Gaps = 18/281 (6%) Frame = +1 Query: 76 GPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMST-GLLTQCVTTRNGTNRN 252 G + VE + +G + ++ VYA+VK + G+ TQCV +N Sbjct: 498 GADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTT 557 Query: 253 DKRRKVVSNKT------VMQIFAKFGYDPWTVEIKLRPTMIVGMDTYH---NKSSKKSIQ 405 + + K V I G P + +P + +G D H K SI Sbjct: 558 PQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGADVTHPPAGDGKKPSIA 613 Query: 406 ASVFSINSTFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVG 585 A V S+++ +Y + V + RQE + L L F K P RI+ +RDGV Sbjct: 614 AVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVS 673 Query: 586 DNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNN 741 + Q Q + E+ A++ E K P I +++V+KR T+LF + N Sbjct: 674 EGQFQQVLHHELLAIR---EACIKLEKEYQPGITFIVVQKRHHTRLFCTDKNERVGKSGN 730 Query: 742 PNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLED 864 GT VD +I P ++F+L S +GT+ P++Y+VL D Sbjct: 731 IPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWD 771
>sp|Q9UKV8|I2C2_HUMAN Eukaryotic translation initiation factor 2C 2 (eIF2C 2) (eIF-2C 2) (Argonaute-2) (Slicer protein) (PAZ Piwi domain protein) (PPD) Length = 851 Score = 85.9 bits (211), Expect = 2e-16 Identities = 77/281 (27%), Positives = 122/281 (43%), Gaps = 18/281 (6%) Frame = +1 Query: 76 GPNDVERCLNDAARSGSGCAKMALVFVPDDRVYAKVKSFTMST-GLLTQCVTTRNGTNRN 252 G + VE + +G + ++ VYA+VK + G+ TQCV +N Sbjct: 489 GADSVEPMFRHLKNTYAGLQLVVVILPGKTPVYAEVKRVGDTVLGMATQCVQMKNVQRTT 548 Query: 253 DKRRKVVSNKT------VMQIFAKFGYDPWTVEIKLRPTMIVGMDTYH---NKSSKKSIQ 405 + + K V I G P + +P + +G D H K SI Sbjct: 549 PQTLSNLCLKINVKLGGVNNILLPQGRPP----VFQQPVIFLGADVTHPPAGDGKKPSIA 604 Query: 406 ASVFSINSTFTQYMSFVNQPRGRQEFHETLGKNFNLALEDFKKRYDILPQRILVFRDGVG 585 A V S+++ +Y + V + RQE + L L F K P RI+ +RDGV Sbjct: 605 AVVGSMDAHPNRYCATVRVQQHRQEIIQDLAAMVRELLIQFYKSTRFKPTRIIFYRDGVS 664 Query: 586 DNQLQFTKNFEVDAMKPLIENIYKSNGFAVPQIIYVIVKKRVGTKLF--------NRGNN 741 + Q Q + E+ A++ E K P I +++V+KR T+LF + N Sbjct: 665 EGQFQQVLHHELLAIR---EACIKLEKDYQPGITFIVVQKRHHTRLFCTDKNERVGKSGN 721 Query: 742 PNPGTVVDKEIVKPNFYEFFLVSQRTTKGTATPTNYNVLED 864 GT VD +I P ++F+L S +GT+ P++Y+VL D Sbjct: 722 IPAGTTVDTKITHPTEFDFYLCSHAGIQGTSRPSHYHVLWD 762
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 113,529,449 Number of Sequences: 369166 Number of extensions: 2379660 Number of successful extensions: 5712 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 5472 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 5682 length of database: 68,354,980 effective HSP length: 111 effective length of database: 47,849,395 effective search space used: 9856975370 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)