Planaria EST Database


DrC_01891

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01891
         (714 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P08962|CD63_HUMAN  CD63 antigen (Melanoma-associated anti...    85   2e-16
sp|P28648|CD63_RAT  CD63 antigen (AD1 antigen)                     81   2e-15
sp|P41731|CD63_MOUSE  CD63 antigen                                 80   4e-15
sp|Q9XSK2|CD63_BOVIN  CD63 antigen                                 80   4e-15
sp|Q28709|CD63_RABIT  CD63 antigen                                 80   5e-15
sp|O60637|TSN3_HUMAN  Tetraspanin-3 (Tspan-3) (Transmembrane...    76   8e-14
sp|Q9QY33|TSN3_MOUSE  Tetraspanin-3 (Tspan-3) (Transmembrane...    76   1e-13
sp|Q9DCK3|TSN4_MOUSE  Tetraspanin-4 (Tspan-4) (Transmembrane...    73   7e-13
sp|P19331|IM23_SCHMA  23 kDa integral membrane protein (SM23)      73   7e-13
sp|Q62283|TSN7_MOUSE  Tetraspanin-7 (Tspan-7) (Transmembrane...    72   1e-12
>sp|P08962|CD63_HUMAN CD63 antigen (Melanoma-associated antigen ME491)
           (Lysosome-associated membrane glycoprotein 3) (LAMP-3)
           (Ocular melanoma-associated antigen) (OMA81H)
           (Granulophysin) (Tetraspanin-30) (Tspan-30)
          Length = 238

 Score = 84.7 bits (208), Expect = 2e-16
 Identities = 51/206 (24%), Positives = 98/206 (47%), Gaps = 10/206 (4%)
 Frame = +1

Query: 25  TYVKYLTKAIQLPILPALGLVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLF 204
           T ++  T    LP++     ++ VG+F+ +++ +G    C    CL+ TF I L +I+L 
Sbjct: 42  TIIQGATPGSLLPVV-----IIAVGVFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLV 96

Query: 205 QFAIAVSTFVLKNNIVKEFKKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRT 384
           + A A++ +V ++ ++ EF      +F +   N   + +  +  + +Q+ FKCCG  N T
Sbjct: 97  EVAAAIAGYVFRDKVMSEFNN----NFRQQMENYPKNNHTASILDRMQADFKCCGAANYT 152

Query: 385 DY----SNKNMSIPSSCCLK-TEDCNIN----TAYTKGCLEDSKSYVEGILVSADLVXXX 537
           D+    S     +P SCC+  T  C IN      + +GC+E    ++   ++        
Sbjct: 153 DWEKIPSMSKNRVPDSCCINVTVGCGINFNEKAIHKEGCVEKIGGWLRKNVLVVAAAALG 212

Query: 538 XXXXXXXXXXFTFCLVRNLK-GYTMV 612
                     F  CLV++++ GY ++
Sbjct: 213 IAFVEVLGIVFACCLVKSIRSGYEVM 238
>sp|P28648|CD63_RAT CD63 antigen (AD1 antigen)
          Length = 238

 Score = 81.3 bits (199), Expect = 2e-15
 Identities = 47/187 (25%), Positives = 88/187 (47%), Gaps = 10/187 (5%)
 Frame = +1

Query: 82  LVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKNNIVKEF 261
           +++ VG F+ +++ +G    C    CL+ TF I L +I+L + A+A++ +V ++ +  EF
Sbjct: 56  VIIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEF 115

Query: 262 KKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRTDYSN----KNMSIPSSCCL 429
            K    SF+K   N           + +Q   KCCG  N TD+          +P SCC+
Sbjct: 116 SK----SFQKQMQNYLTDNKTATILDKLQKENKCCGASNYTDWERIPGMAKDRVPDSCCI 171

Query: 430 KT-----EDCNINTAYTKGCLEDSKSYVEGILVSADLVXXXXXXXXXXXXXFTFCLVRNL 594
                   D   +T +T+GC+E   +++   ++                  F+ CLV+++
Sbjct: 172 NITVGCGNDFKESTIHTQGCVETIAAWLRKNVLLVAGAALGIAFVEVLGIIFSCCLVKSI 231

Query: 595 K-GYTMV 612
           + GY ++
Sbjct: 232 RSGYEVM 238
>sp|P41731|CD63_MOUSE CD63 antigen
          Length = 238

 Score = 80.5 bits (197), Expect = 4e-15
 Identities = 46/187 (24%), Positives = 87/187 (46%), Gaps = 10/187 (5%)
 Frame = +1

Query: 82  LVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKNNIVKEF 261
           +++ VG F+ +++ +G    C    CL+ TF I L +I+L + A+A++ +V ++ +  EF
Sbjct: 56  VIIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAVAIAGYVFRDQVKSEF 115

Query: 262 KKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRTDYSN----KNMSIPSSCCL 429
            K    SF++   N           + +Q    CCG  N TD+ N        +P SCC+
Sbjct: 116 NK----SFQQQMQNYLKDNKTATILDKLQKENNCCGASNYTDWENIPGMAKDRVPDSCCI 171

Query: 430 KT-----EDCNINTAYTKGCLEDSKSYVEGILVSADLVXXXXXXXXXXXXXFTFCLVRNL 594
                   D   +T +T+GC+E    ++   ++                  F+ CLV+++
Sbjct: 172 NITVGCGNDFKESTIHTQGCVETIAIWLRKNILLVAAAALGIAFVEVLGIIFSCCLVKSI 231

Query: 595 K-GYTMV 612
           + GY ++
Sbjct: 232 RSGYEVM 238
>sp|Q9XSK2|CD63_BOVIN CD63 antigen
          Length = 237

 Score = 80.5 bits (197), Expect = 4e-15
 Identities = 49/199 (24%), Positives = 93/199 (46%), Gaps = 9/199 (4%)
 Frame = +1

Query: 43  TKAIQLPILPALGLVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAV 222
           T +  LP++     ++ VG F+ +++ +G    C    CL+ TF I L +I+L + A A+
Sbjct: 48  TPSFLLPVV-----IIAVGAFLFLVAFVGCCGACKENYCLMITFAIFLSLIMLVEVAAAI 102

Query: 223 STFVLKNNIVKEFKKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRTDYSN-- 396
           + +V ++ +  EF K+    F +   N        +  + +Q  F+CCG  N TD+    
Sbjct: 103 AGYVFRDKVRSEFNKD----FRQQMKNYPKDNQTASILDKMQKDFECCGAANYTDWEKIL 158

Query: 397 -KNMSIPSSCCLK-TEDCNIN----TAYTKGCLEDSKSYVEGILVSADLVXXXXXXXXXX 558
                +P SCC+  T +C IN      +T+GC+E   +++   ++               
Sbjct: 159 AVTNKVPDSCCVNITHNCGINFVVKDIHTEGCVEKIAAWLRKNVLVVVAAALGIAFVEIL 218

Query: 559 XXXFTFCLVRNLK-GYTMV 612
                 CLV++++ GY ++
Sbjct: 219 GIVLACCLVKSIRSGYEVM 237
>sp|Q28709|CD63_RABIT CD63 antigen
          Length = 238

 Score = 80.1 bits (196), Expect = 5e-15
 Identities = 47/187 (25%), Positives = 87/187 (46%), Gaps = 10/187 (5%)
 Frame = +1

Query: 82  LVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKNNIVKEF 261
           +++ VG F+ +++ +G    C    CL+ TF I L +I+L + A A++ +V ++ ++ EF
Sbjct: 56  VIIAVGAFLFLVAFVGCCGTCKENYCLMITFAIFLSLIMLVEVAAAIAGYVFRDKVMSEF 115

Query: 262 KKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRTDYSN----KNMSIPSSCCL 429
            K+    F +   N S         + +Q  F CCG  N TD++         +P SCC+
Sbjct: 116 NKD----FRQQMQNYSTDNQTALILDRMQKDFTCCGAANYTDWATIPGMTRDRVPDSCCV 171

Query: 430 K-TEDC----NINTAYTKGCLEDSKSYVEGILVSADLVXXXXXXXXXXXXXFTFCLVRNL 594
             T  C    N+   Y +GC+E    ++   ++                  F  CLV+++
Sbjct: 172 NVTSGCGVKFNVKDIYVEGCVEKIGLWLRKNVLVVAAAALGIAFVEVLGIVFACCLVKSI 231

Query: 595 K-GYTMV 612
           + GY ++
Sbjct: 232 RSGYEVM 238
>sp|O60637|TSN3_HUMAN Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
           (Tetraspanin TM4-A)
          Length = 253

 Score = 76.3 bits (186), Expect = 8e-14
 Identities = 45/152 (29%), Positives = 80/152 (52%), Gaps = 15/152 (9%)
 Frame = +1

Query: 64  ILPALGLVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKN 243
           ++PA+ +++ VG  + +I L+G         C L+TF IIL+++ + +  + V  +V + 
Sbjct: 51  LIPAV-VIIAVGALLFIIGLIGCCATIRESRCGLATFVIILLLVFVTEVVVVVLGYVYRA 109

Query: 244 NIVKEFKKEMYISFEKAYS--NSSDSEYATNFFNYIQSSFKCCGIYNRTDYSN------- 396
            +  E  +    S +K Y   N ++ + A+   +Y+Q    CCGI+N +D+ N       
Sbjct: 110 KVENEVDR----SIQKVYKTYNGTNPDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKET 165

Query: 397 KNMSIPSSCCLKT-EDCNINTA-----YTKGC 474
           KN S+P SCC +T  +CN + A     Y +GC
Sbjct: 166 KNQSVPLSCCRETASNCNGSLAHPSDLYAEGC 197
>sp|Q9QY33|TSN3_MOUSE Tetraspanin-3 (Tspan-3) (Transmembrane 4 superfamily member 8)
           (Tetraspanin TM4-A) (OSP-associated protein 1) (OAP-1)
          Length = 253

 Score = 75.9 bits (185), Expect = 1e-13
 Identities = 45/152 (29%), Positives = 79/152 (51%), Gaps = 15/152 (9%)
 Frame = +1

Query: 64  ILPALGLVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKN 243
           + PA+ +++ VG  + +I L+G         C L+TF  IL+++ + +  + V  +V + 
Sbjct: 51  LFPAV-VIIAVGALLFIIGLIGCCATIRESRCGLATFVFILLLVFVTEVVVVVLGYVYRA 109

Query: 244 NIVKEFKKEMYISFEKAYS--NSSDSEYATNFFNYIQSSFKCCGIYNRTDYSN------- 396
            +  E  +    S +K Y   N ++S+ A+   +Y+Q    CCGI+N +D+ N       
Sbjct: 110 KVENEVDR----SIQKVYKTYNGTNSDAASRAIDYVQRQLHCCGIHNYSDWENTDWFKET 165

Query: 397 KNMSIPSSCCLKT-EDCNINTA-----YTKGC 474
           KN S+P SCC +T + CN + A     Y +GC
Sbjct: 166 KNQSVPLSCCRETAKSCNGSLANPSDLYAEGC 197
>sp|Q9DCK3|TSN4_MOUSE Tetraspanin-4 (Tspan-4) (Transmembrane 4 superfamily member 7)
          Length = 238

 Score = 73.2 bits (178), Expect = 7e-13
 Identities = 47/177 (26%), Positives = 87/177 (49%), Gaps = 10/177 (5%)
 Frame = +1

Query: 25  TYVKYLTKAIQLPILPALGLVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLF 204
           T   + T +   P L A  L++  G F++ I  +G +       CLL TF ++L+++ L 
Sbjct: 37  TQGNFATLSSSFPSLSAANLLIVTGTFVMAIGFVGCIGALKENKCLLLTFFVLLLLVFLL 96

Query: 205 QFAIAVSTFVLKNNI----VKEFKKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGI 372
           +  IAV  F   + I     ++ KK +++        +  +   TN ++ IQ+ F+CCG+
Sbjct: 97  EATIAVLFFAYSDKIDSYAQQDLKKGLHL------YGTQGNVGLTNAWSIIQTDFRCCGV 150

Query: 373 YNRTDYSN--KNMSIPSSCCLKTED-CNIN---TAYTKGCLEDSKSYVEGILVSADL 525
            N TD+        +P SCCL+  D C ++   T +   C E  K++++  L++  +
Sbjct: 151 SNYTDWFEVYNATRVPDSCCLEFSDSCGLHEPGTWWKSPCYETVKAWLQENLLAVGI 207
>sp|P19331|IM23_SCHMA 23 kDa integral membrane protein (SM23)
          Length = 218

 Score = 73.2 bits (178), Expect = 7e-13
 Identities = 51/176 (28%), Positives = 79/176 (44%)
 Frame = +1

Query: 85  VMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKNNIVKEFK 264
           ++ VG+ I+++S LG         C+L  +   L+V+L+ + A A+   V K+ I  E  
Sbjct: 58  IIVVGVIILIVSFLGCCGAIKENVCMLYMYAFFLVVLLIAELAAAIVAVVYKDRIDSEID 117

Query: 265 KEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRTDYSNKNMSIPSSCCLKTEDC 444
             M  + +K       ++  T F N IQSSF CCG     DY     ++P+SC  K E  
Sbjct: 118 ALMTGALDK------PTKEITEFMNLIQSSFHCCGAKGPDDYRG---NVPASC--KEE-- 164

Query: 445 NINTAYTKGCLEDSKSYVEGILVSADLVXXXXXXXXXXXXXFTFCLVRNLKGYTMV 612
             N  YT+GC+    ++++  LV    V                CL R +K Y  V
Sbjct: 165 --NLTYTEGCVSVFGAFLKRNLVIVACVAFGVCFFQLLSIVIACCLGRQIKEYENV 218
>sp|Q62283|TSN7_MOUSE Tetraspanin-7 (Tspan-7) (Transmembrane 4 superfamily member 2)
           (Cell surface glycoprotein A15) (PE31) (TALLA homolog)
          Length = 249

 Score = 72.0 bits (175), Expect = 1e-12
 Identities = 41/154 (26%), Positives = 74/154 (48%), Gaps = 14/154 (9%)
 Frame = +1

Query: 82  LVMGVGIFIIVISLLGMLTGCTAKPCLLSTFGIILIVILLFQFAIAVSTFVLKNNIVKEF 261
           +++G G  I+V  L G    C   P +L  + + L ++ L +    +S FV ++ I   F
Sbjct: 61  VLIGTGTTIVVFGLFGCFATCRGSPWMLKLYAMFLSLVFLAELVAGISGFVFRHEIKDTF 120

Query: 262 KKEMYISFEKAYSNSSDSEYATNFFNYIQSSFKCCGIYNRTDYSNK----NMSIPSSCCL 429
            +    ++  A  N + ++  +   +++Q S  CCG+ N T++S+     +  IP SCC+
Sbjct: 121 LR----TYTDAMQNYNGNDERSRAVDHVQRSLSCCGVQNYTNWSSSPYFLDHGIPPSCCM 176

Query: 430 KTEDC------NINTAYT----KGCLEDSKSYVE 501
              DC      N+  A T    KGC +   S++E
Sbjct: 177 NETDCNPLDLHNLTVAATKVNQKGCYDLVTSFME 210
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 69,912,815
Number of Sequences: 369166
Number of extensions: 1269425
Number of successful extensions: 3659
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3542
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3629
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6316483550
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)