Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_01741
(857 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O95922|TTLL1_HUMAN Tubulin tyrosine ligase-like protein 1 415 e-116
sp|Q91V51|TTLL1_MOUSE Tubulin tyrosine ligase-like protein 1 414 e-115
sp|Q9BWV7|TTLL2_HUMAN Tubulin tyrosine ligase-like protein ... 143 5e-34
sp|Q80UG8|TTLL4_MOUSE Tubulin tyrosine ligase-like protein 4 140 6e-33
sp|Q14679|TTLL4_HUMAN Tubulin tyrosine ligase-like protein 4 139 1e-32
sp|Q6ZT98|TTLL7_HUMAN Tubulin tyrosine ligase-like protein ... 108 2e-23
sp|Q9Y4R7|TTLL3_HUMAN Tubulin tyrosine ligase-like protein ... 85 2e-16
sp|Q8NHH1|TTL11_HUMAN Tubulin tyrosine ligase-like protein 11 81 4e-15
sp|Q8NG68|TTL_HUMAN Tubulin--tyrosine ligase (TTL) 63 9e-10
sp|Q9QXJ0|TTL_RAT Tubulin--tyrosine ligase (TTL) 63 9e-10
>sp|O95922|TTLL1_HUMAN Tubulin tyrosine ligase-like protein 1
Length = 423
Score = 415 bits (1066), Expect = e-116
Identities = 179/266 (67%), Positives = 233/266 (87%)
Frame = +1
Query: 58 KLKYACDFDKSVLLNNFDARGWINVAPDEDWNFYWATVTTIRSIYNVDNGYRLSDDQVLN 237
K+K+ D +KSVL+NNF+ RGW+ V +EDWNFYW +V TIR++++V+ GYRLSDDQ++N
Sbjct: 4 KVKWVTDIEKSVLINNFEKRGWVQVTENEDWNFYWMSVQTIRNVFSVEAGYRLSDDQIVN 63
Query: 238 HFQNHYELTRKDLMVKNIKRYRKELERDGNILAEKDDEGRYIYLDFIPTTFMLPQDYNLF 417
HF NHYELTRKDLMVKNIKRYRKELE++G+ LAEKD+ G+Y+YLDF+P T+MLP DYNLF
Sbjct: 64 HFPNHYELTRKDLMVKNIKRYRKELEKEGSPLAEKDENGKYLYLDFVPVTYMLPADYNLF 123
Query: 418 AEEFKRNPNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYVISRY 597
EEF+++P+STWI+KP KA+G GIF++NKLSQ+KKWSRDS+T++F +K++YVIS Y
Sbjct: 124 VEEFRKSPSSTWIMKPCGKAQGKGIFLINKLSQIKKWSRDSKTSSFVSQSNKEAYVISLY 183
Query: 598 IENPLLIGGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDNLFVHLTNV 777
I NPLLIGG+KFDLR+YVLV+++RPL+ Y+Y+LGFCRFCTVKY P+ +E+DN+FVHLTNV
Sbjct: 184 INNPLLIGGRKFDLRLYVLVSTYRPLRCYMYKLGFCRFCTVKYTPSTSELDNMFVHLTNV 243
Query: 778 SIQKQGSDYNSIHGGKWTIQNFRLWI 855
+IQK G DYN IHGGKWT+ N RL++
Sbjct: 244 AIQKHGEDYNHIHGGKWTVSNLRLYL 269
>sp|Q91V51|TTLL1_MOUSE Tubulin tyrosine ligase-like protein 1
Length = 423
Score = 414 bits (1065), Expect = e-115
Identities = 179/266 (67%), Positives = 233/266 (87%)
Frame = +1
Query: 58 KLKYACDFDKSVLLNNFDARGWINVAPDEDWNFYWATVTTIRSIYNVDNGYRLSDDQVLN 237
++K+ D +KSVL+NNF+ RGWI V +EDWNFYW +V TIR++++V+ GYRLSDDQ++N
Sbjct: 4 RVKWVTDIEKSVLINNFEKRGWIQVTENEDWNFYWMSVQTIRNVFSVETGYRLSDDQIVN 63
Query: 238 HFQNHYELTRKDLMVKNIKRYRKELERDGNILAEKDDEGRYIYLDFIPTTFMLPQDYNLF 417
HF NHYELTRKDLMVKNIKRYRKELE++G+ LAEKD+ G+Y+YLDF+P T+MLP DYNLF
Sbjct: 64 HFPNHYELTRKDLMVKNIKRYRKELEKEGSPLAEKDENGKYLYLDFVPVTYMLPADYNLF 123
Query: 418 AEEFKRNPNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYVISRY 597
EEF+++P+STWI+KP KA+G GIF++NKLSQ+KKWSRDS+T++F +K++YVIS Y
Sbjct: 124 VEEFRKSPSSTWIMKPCGKAQGKGIFLINKLSQIKKWSRDSKTSSFVSQSTKEAYVISVY 183
Query: 598 IENPLLIGGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDNLFVHLTNV 777
I NPLLIGG+KFDLR+YVLV+++RPL+ Y+Y+LGFCRFCTVKY P+ +E+DN+FVHLTNV
Sbjct: 184 INNPLLIGGRKFDLRLYVLVSTYRPLRCYMYKLGFCRFCTVKYTPSTSELDNMFVHLTNV 243
Query: 778 SIQKQGSDYNSIHGGKWTIQNFRLWI 855
+IQK G DYN IHGGKWT+ N RL++
Sbjct: 244 AIQKHGEDYNHIHGGKWTVNNLRLYL 269
>sp|Q9BWV7|TTLL2_HUMAN Tubulin tyrosine ligase-like protein 2 (Testis-specific protein
NYD-TSPG)
Length = 592
Score = 143 bits (361), Expect = 5e-34
Identities = 97/268 (36%), Positives = 131/268 (48%), Gaps = 15/268 (5%)
Frame = +1
Query: 85 KSVLLNNFDARGWINVAPDE----DWNFYWATVTTIRSIYNVDNGYRLSDDQVLNHFQNH 252
+SVLL RGW E DWN YW T S + + + Q LNH
Sbjct: 100 QSVLLE----RGWNKFDKQEQNAEDWNLYWRT-----SSFRMTEHNSVKPWQQLNHHPGT 150
Query: 253 YELTRKDLMVKNIKRYRKELERDGNILAEKDDEGRYIYLDFIPTTFMLPQDYNLFAEEFK 432
+LTRKD + K++K R+ G +Y FIP TF++P DY F E+
Sbjct: 151 TKLTRKDCLAKHLKHMRRMY-------------GTSLY-QFIPLTFVMPNDYTKFVAEYF 196
Query: 433 RN------PNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYVISR 594
+ +S WI KP+ +RG GI I S K + D D Y++ +
Sbjct: 197 QERQMLGTKHSYWICKPAELSRGRGILIF---SDFKDFIFD------------DMYIVQK 241
Query: 595 YIENPLLIGGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDNLFVHLTN 774
YI NPLLIG K DLR+YV VT F+PL +Y+Y+ G RF T K+ +++ + N + HLTN
Sbjct: 242 YISNPLLIGRYKCDLRIYVCVTGFKPLTIYVYQEGLVRFATEKF--DLSNLQNNYAHLTN 299
Query: 775 VSIQKQGSDYNSI-----HGGKWTIQNF 843
SI K G+ Y I HG KWT+ F
Sbjct: 300 SSINKSGASYEKIKEVIGHGCKWTLSRF 327
>sp|Q80UG8|TTLL4_MOUSE Tubulin tyrosine ligase-like protein 4
Length = 934
Score = 140 bits (352), Expect = 6e-33
Identities = 77/223 (34%), Positives = 119/223 (53%), Gaps = 7/223 (3%)
Frame = +1
Query: 205 GYR-LSDDQVLNHFQNHYELTRKDLMVKNIKRYRKELERDGNILAEKDDEGRYIYLDFIP 381
G+R + + Q LNHF +++ RKD + +N+ R + + F P
Sbjct: 385 GFRSIREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKE--------------FSFFP 430
Query: 382 TTFMLPQDYNLFAEEFKRNPNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQL 561
+F+LPQD L + ++ + WIVKP A ARGIGI +++K SQL K
Sbjct: 431 QSFILPQDSKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPK------------ 478
Query: 562 PQSKDSYVISRYIENPLLIGGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVN 741
+ ++ RY+ P LI G KFDLR+YV VTS+ PL++YL+ G RF + KY+P++
Sbjct: 479 ---RRPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMK 535
Query: 742 EIDNLFVHLTNVSIQKQGSDY------NSIHGGKWTIQNFRLW 852
+ N F+HLTN S+ K+ ++Y + G KW ++ LW
Sbjct: 536 SLSNKFMHLTNYSVNKKNTEYQANADETACQGHKWALK--ALW 576
>sp|Q14679|TTLL4_HUMAN Tubulin tyrosine ligase-like protein 4
Length = 1199
Score = 139 bits (349), Expect = 1e-32
Identities = 75/219 (34%), Positives = 116/219 (52%), Gaps = 6/219 (2%)
Frame = +1
Query: 214 LSDDQVLNHFQNHYELTRKDLMVKNIKRYRKELERDGNILAEKDDEGRYIYLDFIPTTFM 393
+ + Q LNHF +++ RKD + +N+ R + + F P +F+
Sbjct: 653 IREHQKLNHFPGSFQIGRKDRLWRNLSRMQSRFGKKE--------------FSFFPQSFI 698
Query: 394 LPQDYNLFAEEFKRNPNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSK 573
LPQD L + ++ + WIVKP A ARGIGI +++K SQL K +
Sbjct: 699 LPQDAKLLRKAWESSSRQKWIVKPPASARGIGIQVIHKWSQLPK---------------R 743
Query: 574 DSYVISRYIENPLLIGGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDN 753
++ RY+ P LI G KFDLR+YV VTS+ PL++YL+ G RF + KY+P++ + N
Sbjct: 744 RPLLVQRYLHKPYLISGSKFDLRIYVYVTSYDPLRIYLFSDGLVRFASCKYSPSMKSLGN 803
Query: 754 LFVHLTNVSIQKQGSDYN------SIHGGKWTIQNFRLW 852
F+HLTN S+ K+ ++Y + G KW ++ LW
Sbjct: 804 KFMHLTNYSVNKKNAEYQANADEMACQGHKWALK--ALW 840
>sp|Q6ZT98|TTLL7_HUMAN Tubulin tyrosine ligase-like protein 7 (Protein NYD-SP30)
Length = 887
Score = 108 bits (269), Expect = 2e-23
Identities = 76/230 (33%), Positives = 108/230 (46%), Gaps = 7/230 (3%)
Frame = +1
Query: 136 PDEDW--NFYWATVTTIRSIYNVDNGYRLSDDQVLNHFQNHYELTRKDLMVKNIKRYRKE 309
PDED N W S + L + Q +NHF E+ RKD + +N+ + K
Sbjct: 66 PDEDETSNLIWCD-----SAVQQEKISELQNYQRINHFPGMGEICRKDFLARNMTKMIKS 120
Query: 310 LERDGNILAEKDDEGRYIYLDFIPTTFMLPQDYNLFA----EEFKRNPNSTWIVKPSAKA 477
D F+P T++ P +Y F E K+ T+IVKP+ A
Sbjct: 121 RPLD---------------YTFVPRTWIFPAEYTQFQNYVKELKKKRKQKTFIVKPANGA 165
Query: 478 RGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYVISRYIENPLLIGGKKFDLRMYVLV 657
G GI ++ N +LP S+D ++ YIE P L+ G KFDLR+Y+LV
Sbjct: 166 MGHGISLIR--------------NGDKLP-SQDHLIVQEYIEKPFLMEGYKFDLRIYILV 210
Query: 658 TSFRPLKVYLYRLGFCRFCTVKY-NPNVNEIDNLFVHLTNVSIQKQGSDY 804
TS PLK++LY G R T KY PN + + L++HLTN S+ K +
Sbjct: 211 TSCDPLKIFLYHDGLVRMGTEKYIPPNESNLTQLYMHLTNYSVNKHNEHF 260
>sp|Q9Y4R7|TTLL3_HUMAN Tubulin tyrosine ligase-like protein 3 (HOTTL)
Length = 352
Score = 85.1 bits (209), Expect = 2e-16
Identities = 55/139 (39%), Positives = 74/139 (53%), Gaps = 7/139 (5%)
Frame = +1
Query: 451 WIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYVISRYIENPLLIGGKK 630
WIVKP AK+RG GI ++ L ++ K N + +V+ +YIE PLLI G K
Sbjct: 68 WIVKPGAKSRGRGIMCMDHLEEMLKL-----VNGNPVVMKDGKWVVQKYIERPLLIFGTK 122
Query: 631 FDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDNLFVHLTNVSIQKQGSDYNS 810
FDLR + LVT + PL V+ YR + RF T + ++ +DN VHL N SIQK NS
Sbjct: 123 FDLRQWFLVTDWNPLTVWFYRDSYIRFSTQPF--SLKNLDN-SVHLCNNSIQKHLE--NS 177
Query: 811 IH-------GGKWTIQNFR 846
H W+ Q F+
Sbjct: 178 CHRHPLLPPDNMWSSQRFQ 196
>sp|Q8NHH1|TTL11_HUMAN Tubulin tyrosine ligase-like protein 11
Length = 538
Score = 80.9 bits (198), Expect = 4e-15
Identities = 48/152 (31%), Positives = 82/152 (53%), Gaps = 7/152 (4%)
Frame = +1
Query: 370 DFIPTTFMLPQDYNLFAEEFK--RNPNSTW----IVKPSAKARGIGIFILNKLSQLKKWS 531
+F P +++LP ++ LF + + ++ + +W IVKP +G GI+++ S ++
Sbjct: 303 NFYPRSWILPDEFQLFVAQVQMVKDDDPSWKPTFIVKPDGGCQGDGIYLIKDPSDIRLAG 362
Query: 532 RDSRTNNFQLPQSKDSYVISRYIENPLLIGGKKFDLRMYVLVTSFRPLKVYLYRLGFCRF 711
QS+ + V+ YI PLLI KFD+R+YVL+ S PL++Y+ + G RF
Sbjct: 363 T---------LQSRPA-VVQEYICKPLLIDKLKFDIRLYVLLKSLDPLEIYIAKDGLSRF 412
Query: 712 CTVKY-NPNVNEIDNLFVHLTNVSIQKQGSDY 804
CT Y P + +F+HLTN S+ ++
Sbjct: 413 CTEPYQEPTPKNLHRIFMHLTNYSLNIHSGNF 444
>sp|Q8NG68|TTL_HUMAN Tubulin--tyrosine ligase (TTL)
Length = 377
Score = 63.2 bits (152), Expect = 9e-10
Identities = 50/133 (37%), Positives = 71/133 (53%), Gaps = 2/133 (1%)
Frame = +1
Query: 406 YNLFAEEFKRNPNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYV 585
YN E+ + N WI K SA A+G GI I ++ S+L D N Q+ +V
Sbjct: 135 YNRKKEDGEGN---VWIAKSSAGAKGEGILISSEASEL----LDFIDNQGQV------HV 181
Query: 586 ISRYIENPLLI--GGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDNLF 759
I +Y+E+PLL+ G +KFD+R +VLV +YLYR G R + Y +V+ +
Sbjct: 182 IQKYLEHPLLLEPGHRKFDIRSWVLVD--HQYNIYLYREGVLRTASEPY--HVDNFQDKT 237
Query: 760 VHLTNVSIQKQGS 798
HLTN IQK+ S
Sbjct: 238 CHLTNHCIQKEYS 250
>sp|Q9QXJ0|TTL_RAT Tubulin--tyrosine ligase (TTL)
Length = 377
Score = 63.2 bits (152), Expect = 9e-10
Identities = 50/133 (37%), Positives = 71/133 (53%), Gaps = 2/133 (1%)
Frame = +1
Query: 406 YNLFAEEFKRNPNSTWIVKPSAKARGIGIFILNKLSQLKKWSRDSRTNNFQLPQSKDSYV 585
YN E+ + N WI K SA A+G GI I ++ S+L D N Q+ +V
Sbjct: 135 YNRKKEDGEGN---VWIAKSSAGAKGEGILISSEASEL----LDFIDNQGQV------HV 181
Query: 586 ISRYIENPLLI--GGKKFDLRMYVLVTSFRPLKVYLYRLGFCRFCTVKYNPNVNEIDNLF 759
I +Y+E+PLL+ G +KFD+R +VLV +YLYR G R + Y +V+ +
Sbjct: 182 IQKYLEHPLLLEPGHRKFDIRSWVLVD--HQYNIYLYREGVLRTASEPY--HVDNFQDKT 237
Query: 760 VHLTNVSIQKQGS 798
HLTN IQK+ S
Sbjct: 238 CHLTNHCIQKEYS 250
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,313,058
Number of Sequences: 369166
Number of extensions: 1998679
Number of successful extensions: 5282
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5083
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5254
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8486520240
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)