Planaria EST Database


DrC_01696

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01696
         (756 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P27636|CDC15_YEAST  Cell division control protein 15           152   1e-36
sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...   149   6e-36
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...   147   2e-35
sp|P23561|STE11_YEAST  Serine/threonine-protein kinase STE11      145   1e-34
sp|O22042|M3K3_ARATH  Mitogen-activated protein kinase kinas...   145   1e-34
sp|O00506|STK25_HUMAN  Serine/threonine-protein kinase 25 (S...   144   3e-34
sp|Q9Z2W1|STK25_MOUSE  Serine/threonine-protein kinase 25 (S...   143   6e-34
sp|Q9Y6E0|STK24_HUMAN  Serine/threonine-protein kinase 24 (S...   139   8e-33
sp|Q61084|M3K3_MOUSE  Mitogen-activated protein kinase kinas...   139   8e-33
sp|Q99KH8|STK24_MOUSE  Serine/threonine-protein kinase 24         139   8e-33
>sp|P27636|CDC15_YEAST Cell division control protein 15
          Length = 974

 Score =  152 bits (383), Expect = 1e-36
 Identities = 85/202 (42%), Positives = 117/202 (57%), Gaps = 2/202 (0%)
 Frame = -3

Query: 691 HKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKE--GMVEINKIIRYSKQILVGLQFL 518
           H NIV++ G + KS   YI +EY + GSL+ LI +   G+ E N+   Y  Q L+GL++L
Sbjct: 79  HNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSE-NESKTYVTQTLLGLKYL 137

Query: 517 HSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEIF 338
           H + ++HRDIK ANILL  D TVKL+DFG+S  +  +       A T  GT N++APEI 
Sbjct: 138 HGEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSS-------ALTLAGTLNWMAPEIL 190

Query: 337 LGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPMKS 158
                   +DIWS GATV+ M+T N PYH L    I + +   +   + P    S+P+K 
Sbjct: 191 GNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAV---ENDTYYPPSSFSEPLKD 247

Query: 157 FIKKLLTANQLKRPTAESLLNH 92
           F+ K    N  KRPTA+ LL H
Sbjct: 248 FLSKCFVKNMYKRPTADQLLKH 269
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score =  149 bits (377), Expect = 6e-36
 Identities = 82/208 (39%), Positives = 119/208 (57%), Gaps = 1/208 (0%)
 Frame = -3

Query: 712 KFSSQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILV 533
           K    + H NIVR+ G + +     I +E++ GGS+  L++K G    + +  Y+ Q+L+
Sbjct: 123 KLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLL 182

Query: 532 GLQFLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYL 353
           GL++LH+  I+HRDIK ANIL+D    +KL+DFG S+ + +    ++  A +  GTP ++
Sbjct: 183 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAEL--ATISGAKSMKGTPYWM 240

Query: 352 APEIFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPE-AIIFKIGKSKKIEFSPSQPI 176
           APE+ L   + +SADIWS G TVI M+TG  P+ Q   E A IF IG +K     P   I
Sbjct: 241 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDN-I 299

Query: 175 SKPMKSFIKKLLTANQLKRPTAESLLNH 92
           S     F+ K L      RPTA  LL H
Sbjct: 300 SSDANDFLLKCLQQEPNLRPTASELLKH 327
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
           NPK1-related protein kinase 1)
          Length = 666

 Score =  147 bits (372), Expect = 2e-35
 Identities = 82/208 (39%), Positives = 121/208 (58%), Gaps = 1/208 (0%)
 Frame = -3

Query: 712 KFSSQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILV 533
           K    + H NIVR+ G + +     I +E++ GGS+  L++K G    + +  Y++Q+L+
Sbjct: 124 KLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLL 183

Query: 532 GLQFLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYL 353
           GL++LH+  I+HRDIK ANIL+D    +KL+DFG S+ + +    ++  A +  GTP ++
Sbjct: 184 GLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAEL--ATMTGAKSMKGTPYWM 241

Query: 352 APEIFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPE-AIIFKIGKSKKIEFSPSQPI 176
           APE+ L   + +SADIWS G TVI M+TG  P+ Q   E A IF IG +K     P   +
Sbjct: 242 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIP-DTL 300

Query: 175 SKPMKSFIKKLLTANQLKRPTAESLLNH 92
           S   K F+ K L      RPTA  LL H
Sbjct: 301 SSDAKDFLLKCLQEVPNLRPTASELLKH 328
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
          Length = 738

 Score =  145 bits (366), Expect = 1e-34
 Identities = 80/207 (38%), Positives = 122/207 (58%)
 Frame = -3

Query: 700  QIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQF 521
            ++ H+NIV ++GA ++ GN  IF+EY+ GGS+  +++  G  E + I  +++QIL+G+ +
Sbjct: 531  ELHHENIVTYYGASQEGGNLNIFLEYVPGGSVSSMLNNYGPFEESLITNFTRQILIGVAY 590

Query: 520  LHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEI 341
            LH K I+HRDIK ANIL+D    VK++DFGIS+ L    ++  +K  +  G+  +++PE+
Sbjct: 591  LHKKNIIHRDIKGANILIDIKGCVKITDFGISKKL-SPLNKKQNKRASLQGSVFWMSPEV 649

Query: 340  FLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPMK 161
                     ADIWSTG  VI M TG  P+        IFKIG +   E  PS   S+  K
Sbjct: 650  VKQTATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIGTNTTPEI-PSWATSEG-K 707

Query: 160  SFIKKLLTANQLKRPTAESLLNHSLFD 80
            +F++K    +   RP+A  LL H   D
Sbjct: 708  NFLRKAFELDYQYRPSALELLQHPWLD 734
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
           NPK1-related protein kinase 3)
          Length = 651

 Score =  145 bits (365), Expect = 1e-34
 Identities = 81/201 (40%), Positives = 121/201 (60%), Gaps = 1/201 (0%)
 Frame = -3

Query: 691 HKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQFLHS 512
           H NIVR+ G + +S +  I ME++ GGS+  L++K G      II Y+KQ+L+GL++LH+
Sbjct: 130 HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHN 189

Query: 511 KTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEIFLG 332
             I+HRDIK ANIL+D    ++L+DFG S+ + +    +V+ A +  GTP ++APE+ L 
Sbjct: 190 NGIMHRDIKGANILVDNKGCIRLADFGASKKVVEL--ATVNGAKSMKGTPYWMAPEVILQ 247

Query: 331 DPYDYSADIWSTGATVIHMITGNMPY-HQLRPEAIIFKIGKSKKIEFSPSQPISKPMKSF 155
             + +SADIWS G TVI M TG  P+  Q +  A +  IG++K     P + +S   K F
Sbjct: 248 TGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIP-EDLSPEAKDF 306

Query: 154 IKKLLTANQLKRPTAESLLNH 92
           + K L      R +A  LL H
Sbjct: 307 LMKCLHKEPSLRLSATELLQH 327
>sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (Sterile 20/oxidant
           stress-response kinase 1) (Ste20/oxidant stress response
           kinase-1) (SOK-1) (Ste20-like kinase)
          Length = 426

 Score =  144 bits (363), Expect = 3e-34
 Identities = 84/204 (41%), Positives = 112/204 (54%)
 Frame = -3

Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
           SQ     I R+FG+  KS   +I MEY+ GGS  DL+ K G +E   I    ++IL GL 
Sbjct: 71  SQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLL-KPGPLEETYIATILREILKGLD 129

Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
           +LHS+  +HRDIK AN+LL     VKL+DFG++  L  T      K +TF GTP ++APE
Sbjct: 130 YLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 185

Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
           +     YD+ ADIWS G T I +  G  P   L P  ++F I K+           SKP 
Sbjct: 186 VIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQH--SKPF 243

Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
           K F++  L  +   RPTA+ LL H
Sbjct: 244 KEFVEACLNKDPRFRPTAKELLKH 267
>sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (Sterile 20/oxidant
           stress-response kinase 1) (Ste20/oxidant stress response
           kinase-1) (SOK-1) (Ste20-like kinase)
          Length = 426

 Score =  143 bits (360), Expect = 6e-34
 Identities = 83/204 (40%), Positives = 112/204 (54%)
 Frame = -3

Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
           SQ     I R+FG+  KS   +I MEY+ GGS  DL+ K G +E   I    ++IL GL 
Sbjct: 71  SQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLL-KPGPLEETYIATILREILKGLD 129

Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
           +LHS+  +HRDIK AN+LL     VK++DFG++  L  T      K +TF GTP ++APE
Sbjct: 130 YLHSERKIHRDIKAANVLLSEQGDVKMADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 185

Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
           +     YD+ ADIWS G T I +  G  P   L P  ++F I K+           SKP 
Sbjct: 186 VIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNNPPTLEGHH--SKPF 243

Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
           K F++  L  +   RPTA+ LL H
Sbjct: 244 KEFVEACLNKDPRFRPTAKELLKH 267
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
           (Mammalian STE20-like protein kinase 3)
          Length = 443

 Score =  139 bits (350), Expect = 8e-33
 Identities = 78/204 (38%), Positives = 114/204 (55%)
 Frame = -3

Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
           SQ     + +++G+  K    +I MEY+ GGS  DL++  G ++  +I    ++IL GL 
Sbjct: 87  SQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDETQIATILREILKGLD 145

Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
           +LHS+  +HRDIK AN+LL     VKL+DFG++  L  T      K +TF GTP ++APE
Sbjct: 146 YLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 201

Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
           +     YD  ADIWS G T I +  G  P+ +L P  ++F I K+       +   SKP+
Sbjct: 202 VIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGN--YSKPL 259

Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
           K F++  L      RPTA+ LL H
Sbjct: 260 KEFVEACLNKEPSFRPTAKELLKH 283
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
            kinase 3) (MEK kinase 3) (MEKK 3)
          Length = 626

 Score =  139 bits (350), Expect = 8e-33
 Identities = 81/202 (40%), Positives = 116/202 (57%), Gaps = 2/202 (0%)
 Frame = -3

Query: 691  HKNIVRHFGALEKSGN--FYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQFL 518
            H+ IV+++G L         IFMEY+ GGS+KD +   G +  +   +Y++QIL G+ +L
Sbjct: 421  HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480

Query: 517  HSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEIF 338
            HS  IVHRDIK ANIL D    VKL DFG S+ LQ T   S     +  GTP +++PE+ 
Sbjct: 481  HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQ-TICMSGTGIRSVTGTPYWMSPEVI 539

Query: 337  LGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPMKS 158
             G+ Y   AD+WS G TV+ M+T   P+ +    A IFKI         PS  IS+  + 
Sbjct: 540  SGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSH-ISEHGRD 598

Query: 157  FIKKLLTANQLKRPTAESLLNH 92
            F++++    + +RP+AE LL H
Sbjct: 599  FLRRIFVEAR-QRPSAEELLTH 619
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
          Length = 431

 Score =  139 bits (350), Expect = 8e-33
 Identities = 79/204 (38%), Positives = 114/204 (55%)
 Frame = -3

Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
           SQ     + +++G+  K    +I MEY+ GGS  DL++   + EI +I    ++IL GL 
Sbjct: 75  SQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEPGPLDEI-QIATILREILKGLD 133

Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
           +LHS+  +HRDIK AN+LL     VKL+DFG++  L  T      K +TF GTP ++APE
Sbjct: 134 YLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 189

Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
           +     YD  ADIWS G T I +  G  P+ +L P  ++F I K+       +   SKP+
Sbjct: 190 VIKQSAYDSKADIWSLGITAIELAKGEPPHSELHPMKVLFLIPKNNPPTLEGN--YSKPL 247

Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
           K F++  L      RPTA+ LL H
Sbjct: 248 KEFVEACLNKEPSFRPTAKELLKH 271
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,364,061
Number of Sequences: 369166
Number of extensions: 1395138
Number of successful extensions: 7691
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5563
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6921714800
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)