Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_01696
(756 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P27636|CDC15_YEAST Cell division control protein 15 152 1e-36
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 149 6e-36
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 147 2e-35
sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11 145 1e-34
sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinas... 145 1e-34
sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (S... 144 3e-34
sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (S... 143 6e-34
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 139 8e-33
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 139 8e-33
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 139 8e-33
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 152 bits (383), Expect = 1e-36
Identities = 85/202 (42%), Positives = 117/202 (57%), Gaps = 2/202 (0%)
Frame = -3
Query: 691 HKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKE--GMVEINKIIRYSKQILVGLQFL 518
H NIV++ G + KS YI +EY + GSL+ LI + G+ E N+ Y Q L+GL++L
Sbjct: 79 HNNIVKYHGFIRKSYELYILLEYCANGSLRRLISRSSTGLSE-NESKTYVTQTLLGLKYL 137
Query: 517 HSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEIF 338
H + ++HRDIK ANILL D TVKL+DFG+S + + A T GT N++APEI
Sbjct: 138 HGEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSS-------ALTLAGTLNWMAPEIL 190
Query: 337 LGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPMKS 158
+DIWS GATV+ M+T N PYH L I + + + + P S+P+K
Sbjct: 191 GNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAV---ENDTYYPPSSFSEPLKD 247
Query: 157 FIKKLLTANQLKRPTAESLLNH 92
F+ K N KRPTA+ LL H
Sbjct: 248 FLSKCFVKNMYKRPTADQLLKH 269
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 149 bits (377), Expect = 6e-36
Identities = 82/208 (39%), Positives = 119/208 (57%), Gaps = 1/208 (0%)
Frame = -3
Query: 712 KFSSQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILV 533
K + H NIVR+ G + + I +E++ GGS+ L++K G + + Y+ Q+L+
Sbjct: 123 KLLKNLSHPNIVRYLGTVREDETLNILLEFVPGGSISSLLEKFGAFPESVVRTYTNQLLL 182
Query: 532 GLQFLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYL 353
GL++LH+ I+HRDIK ANIL+D +KL+DFG S+ + + ++ A + GTP ++
Sbjct: 183 GLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQVAEL--ATISGAKSMKGTPYWM 240
Query: 352 APEIFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPE-AIIFKIGKSKKIEFSPSQPI 176
APE+ L + +SADIWS G TVI M+TG P+ Q E A IF IG +K P I
Sbjct: 241 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEIAAIFHIGTTKSHPPIPDN-I 299
Query: 175 SKPMKSFIKKLLTANQLKRPTAESLLNH 92
S F+ K L RPTA LL H
Sbjct: 300 SSDANDFLLKCLQQEPNLRPTASELLKH 327
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 147 bits (372), Expect = 2e-35
Identities = 82/208 (39%), Positives = 121/208 (58%), Gaps = 1/208 (0%)
Frame = -3
Query: 712 KFSSQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILV 533
K + H NIVR+ G + + I +E++ GGS+ L++K G + + Y++Q+L+
Sbjct: 124 KLLKNLSHPNIVRYLGTVREDDTLNILLEFVPGGSISSLLEKFGPFPESVVRTYTRQLLL 183
Query: 532 GLQFLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYL 353
GL++LH+ I+HRDIK ANIL+D +KL+DFG S+ + + ++ A + GTP ++
Sbjct: 184 GLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQVAEL--ATMTGAKSMKGTPYWM 241
Query: 352 APEIFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPE-AIIFKIGKSKKIEFSPSQPI 176
APE+ L + +SADIWS G TVI M+TG P+ Q E A IF IG +K P +
Sbjct: 242 APEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKEVAAIFFIGTTKSHPPIP-DTL 300
Query: 175 SKPMKSFIKKLLTANQLKRPTAESLLNH 92
S K F+ K L RPTA LL H
Sbjct: 301 SSDAKDFLLKCLQEVPNLRPTASELLKH 328
>sp|P23561|STE11_YEAST Serine/threonine-protein kinase STE11
Length = 738
Score = 145 bits (366), Expect = 1e-34
Identities = 80/207 (38%), Positives = 122/207 (58%)
Frame = -3
Query: 700 QIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQF 521
++ H+NIV ++GA ++ GN IF+EY+ GGS+ +++ G E + I +++QIL+G+ +
Sbjct: 531 ELHHENIVTYYGASQEGGNLNIFLEYVPGGSVSSMLNNYGPFEESLITNFTRQILIGVAY 590
Query: 520 LHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEI 341
LH K I+HRDIK ANIL+D VK++DFGIS+ L ++ +K + G+ +++PE+
Sbjct: 591 LHKKNIIHRDIKGANILIDIKGCVKITDFGISKKL-SPLNKKQNKRASLQGSVFWMSPEV 649
Query: 340 FLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPMK 161
ADIWSTG VI M TG P+ IFKIG + E PS S+ K
Sbjct: 650 VKQTATTAKADIWSTGCVVIEMFTGKHPFPDFSQMQAIFKIGTNTTPEI-PSWATSEG-K 707
Query: 160 SFIKKLLTANQLKRPTAESLLNHSLFD 80
+F++K + RP+A LL H D
Sbjct: 708 NFLRKAFELDYQYRPSALELLQHPWLD 734
>sp|O22042|M3K3_ARATH Mitogen-activated protein kinase kinase kinase 3 (Arabidospsis
NPK1-related protein kinase 3)
Length = 651
Score = 145 bits (365), Expect = 1e-34
Identities = 81/201 (40%), Positives = 121/201 (60%), Gaps = 1/201 (0%)
Frame = -3
Query: 691 HKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQFLHS 512
H NIVR+ G + +S + I ME++ GGS+ L++K G II Y+KQ+L+GL++LH+
Sbjct: 130 HPNIVRYLGTVRESDSLNILMEFVPGGSISSLLEKFGSFPEPVIIMYTKQLLLGLEYLHN 189
Query: 511 KTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEIFLG 332
I+HRDIK ANIL+D ++L+DFG S+ + + +V+ A + GTP ++APE+ L
Sbjct: 190 NGIMHRDIKGANILVDNKGCIRLADFGASKKVVEL--ATVNGAKSMKGTPYWMAPEVILQ 247
Query: 331 DPYDYSADIWSTGATVIHMITGNMPY-HQLRPEAIIFKIGKSKKIEFSPSQPISKPMKSF 155
+ +SADIWS G TVI M TG P+ Q + A + IG++K P + +S K F
Sbjct: 248 TGHSFSADIWSVGCTVIEMATGKPPWSEQYQQFAAVLHIGRTKAHPPIP-EDLSPEAKDF 306
Query: 154 IKKLLTANQLKRPTAESLLNH 92
+ K L R +A LL H
Sbjct: 307 LMKCLHKEPSLRLSATELLQH 327
>sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 144 bits (363), Expect = 3e-34
Identities = 84/204 (41%), Positives = 112/204 (54%)
Frame = -3
Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
SQ I R+FG+ KS +I MEY+ GGS DL+ K G +E I ++IL GL
Sbjct: 71 SQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLL-KPGPLEETYIATILREILKGLD 129
Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
+LHS+ +HRDIK AN+LL VKL+DFG++ L T K +TF GTP ++APE
Sbjct: 130 YLHSERKIHRDIKAANVLLSEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 185
Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
+ YD+ ADIWS G T I + G P L P ++F I K+ SKP
Sbjct: 186 VIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQH--SKPF 243
Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
K F++ L + RPTA+ LL H
Sbjct: 244 KEFVEACLNKDPRFRPTAKELLKH 267
>sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 143 bits (360), Expect = 6e-34
Identities = 83/204 (40%), Positives = 112/204 (54%)
Frame = -3
Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
SQ I R+FG+ KS +I MEY+ GGS DL+ K G +E I ++IL GL
Sbjct: 71 SQCDSPYITRYFGSYLKSTKLWIIMEYLGGGSALDLL-KPGPLEETYIATILREILKGLD 129
Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
+LHS+ +HRDIK AN+LL VK++DFG++ L T K +TF GTP ++APE
Sbjct: 130 YLHSERKIHRDIKAANVLLSEQGDVKMADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 185
Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
+ YD+ ADIWS G T I + G P L P ++F I K+ SKP
Sbjct: 186 VIKQSAYDFKADIWSLGITAIELAKGEPPNSDLHPMRVLFLIPKNNPPTLEGHH--SKPF 243
Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
K F++ L + RPTA+ LL H
Sbjct: 244 KEFVEACLNKDPRFRPTAKELLKH 267
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 139 bits (350), Expect = 8e-33
Identities = 78/204 (38%), Positives = 114/204 (55%)
Frame = -3
Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
SQ + +++G+ K +I MEY+ GGS DL++ G ++ +I ++IL GL
Sbjct: 87 SQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEP-GPLDETQIATILREILKGLD 145
Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
+LHS+ +HRDIK AN+LL VKL+DFG++ L T K +TF GTP ++APE
Sbjct: 146 YLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 201
Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
+ YD ADIWS G T I + G P+ +L P ++F I K+ + SKP+
Sbjct: 202 VIKQSAYDSKADIWSLGITAIELARGEPPHSELHPMKVLFLIPKNNPPTLEGN--YSKPL 259
Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
K F++ L RPTA+ LL H
Sbjct: 260 KEFVEACLNKEPSFRPTAKELLKH 283
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 139 bits (350), Expect = 8e-33
Identities = 81/202 (40%), Positives = 116/202 (57%), Gaps = 2/202 (0%)
Frame = -3
Query: 691 HKNIVRHFGALEKSGN--FYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQFL 518
H+ IV+++G L IFMEY+ GGS+KD + G + + +Y++QIL G+ +L
Sbjct: 421 HERIVQYYGCLRDRAEKILTIFMEYMPGGSVKDQLKAYGALTESVTRKYTRQILEGMSYL 480
Query: 517 HSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPEIF 338
HS IVHRDIK ANIL D VKL DFG S+ LQ T S + GTP +++PE+
Sbjct: 481 HSNMIVHRDIKGANILRDSAGNVKLGDFGASKRLQ-TICMSGTGIRSVTGTPYWMSPEVI 539
Query: 337 LGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPMKS 158
G+ Y AD+WS G TV+ M+T P+ + A IFKI PS IS+ +
Sbjct: 540 SGEGYGRKADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSH-ISEHGRD 598
Query: 157 FIKKLLTANQLKRPTAESLLNH 92
F++++ + +RP+AE LL H
Sbjct: 599 FLRRIFVEAR-QRPSAEELLTH 619
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 139 bits (350), Expect = 8e-33
Identities = 79/204 (38%), Positives = 114/204 (55%)
Frame = -3
Query: 703 SQIIHKNIVRHFGALEKSGNFYIFMEYISGGSLKDLIDKEGMVEINKIIRYSKQILVGLQ 524
SQ + +++G+ K +I MEY+ GGS DL++ + EI +I ++IL GL
Sbjct: 75 SQCDSPYVTKYYGSYLKDTKLWIIMEYLGGGSALDLLEPGPLDEI-QIATILREILKGLD 133
Query: 523 FLHSKTIVHRDIKPANILLDRDETVKLSDFGISRSLQKTFSQSVDKADTFCGTPNYLAPE 344
+LHS+ +HRDIK AN+LL VKL+DFG++ L T K +TF GTP ++APE
Sbjct: 134 YLHSEKKIHRDIKAANVLLSEHGEVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPE 189
Query: 343 IFLGDPYDYSADIWSTGATVIHMITGNMPYHQLRPEAIIFKIGKSKKIEFSPSQPISKPM 164
+ YD ADIWS G T I + G P+ +L P ++F I K+ + SKP+
Sbjct: 190 VIKQSAYDSKADIWSLGITAIELAKGEPPHSELHPMKVLFLIPKNNPPTLEGN--YSKPL 247
Query: 163 KSFIKKLLTANQLKRPTAESLLNH 92
K F++ L RPTA+ LL H
Sbjct: 248 KEFVEACLNKEPSFRPTAKELLKH 271
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 74,364,061
Number of Sequences: 369166
Number of extensions: 1395138
Number of successful extensions: 7691
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5296
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5563
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6921714800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)