Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_01539
(656 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase st... 71 2e-12
sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2 70 6e-12
sp|P50527|SHK1_SCHPO Serine/threonine-protein kinase pak1/shk1 69 1e-11
sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinas... 68 2e-11
sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p... 67 4e-11
sp|Q8CIN4|PAK2_MOUSE Serine/threonine-protein kinase PAK 2 ... 67 4e-11
sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinas... 67 4e-11
sp|Q13177|PAK2_HUMAN Serine/threonine-protein kinase PAK 2 ... 67 4e-11
sp|Q29502|PAK2_RABIT Serine/threonine-protein kinase PAK 2 ... 67 4e-11
sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinas... 67 5e-11
>sp|P28829|BYR2_SCHPO Protein kinase byr2 (Protein kinase ste8) (MAPK kinase kinase)
(MAPKKK)
Length = 659
Score = 71.2 bits (173), Expect = 2e-12
Identities = 45/132 (34%), Positives = 73/132 (55%), Gaps = 1/132 (0%)
Frame = +3
Query: 141 GSSVLVDNEDNIKLTNFHLSKYL-FNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGL 317
G+++LVDN+ IK+++F +SK L N+T + P + S W+APEV++ +
Sbjct: 525 GANILVDNKGKIKISDFGISKKLELNSTSTKTGGARPS--FQGSSFWMAPEVVKQTMHTE 582
Query: 318 KSDIWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIHYNRNLIPGYFHDFLDRCLRV 497
K+DIWSL V+EM T PYP + ++A RI EN + + I DFL++ +
Sbjct: 583 KTDIWSLGCLVIEMLTSKHPYPNCDQMQAIFRI-GENILPEFPSNISSSAIDFLEKTFAI 641
Query: 498 PHWERPSAKQCI 533
RP+A + +
Sbjct: 642 DCNLRPTASELL 653
>sp|Q10056|SHK2_SCHPO Serine/threonine-protein kinase shk2
Length = 589
Score = 69.7 bits (169), Expect = 6e-12
Identities = 42/131 (32%), Positives = 68/131 (51%), Gaps = 2/131 (1%)
Frame = +3
Query: 147 SVLVDNEDNIKLTNFHLSKYLFNATMDQL-LEGIPGSRIETSKCWLAPEVLRNDPYGLKS 323
+VL+DN NIK+T+F L N T ++ + G P W+APEV++ + YG K
Sbjct: 439 NVLLDNSGNIKITDFGFCARLSNRTNKRVTMVGTP--------YWMAPEVVKQNEYGTKV 490
Query: 324 DIWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIHYNR-NLIPGYFHDFLDRCLRVP 500
DIWSL + ++EM ++ PY + +RA + I + NL+ FL+ CL +
Sbjct: 491 DIWSLGIMIIEMIENEPPYLREDPIRALYLIAKNGTPTLKKPNLVSKNLKSFLNSCLTID 550
Query: 501 HWERPSAKQCI 533
R +A + +
Sbjct: 551 TIFRATAAELL 561
>sp|P50527|SHK1_SCHPO Serine/threonine-protein kinase pak1/shk1
Length = 658
Score = 68.6 bits (166), Expect = 1e-11
Identities = 43/132 (32%), Positives = 66/132 (50%), Gaps = 2/132 (1%)
Frame = +3
Query: 147 SVLVDNEDNIKLTNFHLSKYL-FNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLKS 323
++L+ + +IKLT+F + N T + G P W+APEV+ YG K
Sbjct: 510 NILLSLQGDIKLTDFGFCAQIDSNMTKRTTMVGTP--------YWMAPEVVTRKEYGFKV 561
Query: 324 DIWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIHYNR-NLIPGYFHDFLDRCLRVP 500
D+WSL + +EM + PY N LRA + I +R L+ FHDFL + L V
Sbjct: 562 DVWSLGIMAIEMVEGEPPYLNENPLRALYLIATIGTPKISRPELLSSVFHDFLSKSLTVN 621
Query: 501 HWERPSAKQCIQ 536
+RPS+ + ++
Sbjct: 622 PKQRPSSGELLR 633
>sp|Q61083|M3K2_MOUSE Mitogen-activated protein kinase kinase kinase 2 (MAPK/ERK kinase
kinase 2) (MEK kinase 2) (MEKK 2)
Length = 619
Score = 68.2 bits (165), Expect = 2e-11
Identities = 44/132 (33%), Positives = 68/132 (51%)
Frame = +3
Query: 141 GSSVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLK 320
G+++L D+ NIKL +F SK L L G + + W++PEV+ + YG K
Sbjct: 486 GANILRDSTGNIKLGDFGASKRLQTIC----LSGTGMKSVTGTPYWMSPEVISGEGYGRK 541
Query: 321 SDIWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIHYNRNLIPGYFHDFLDRCLRVP 500
+DIWS+A TV+EM T P+ E + A +I + + Y DFL R + V
Sbjct: 542 ADIWSVACTVVEMLTEKPPWAEFEAMAAIFKIATQPTNPKLPPHVSDYTRDFLKR-IFVE 600
Query: 501 HWERPSAKQCIQ 536
RPSA++ ++
Sbjct: 601 AKLRPSAEELLR 612
>sp|Q64303|PAK2_RAT Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (Gamma-PAK) (p62-PAK)
Length = 524
Score = 67.0 bits (162), Expect = 4e-11
Identities = 45/131 (34%), Positives = 66/131 (50%), Gaps = 1/131 (0%)
Frame = +3
Query: 147 SVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLKSD 326
+VL+ E ++KLT+F + T +Q S + + W+APEV+ YG K D
Sbjct: 373 NVLLGMEGSVKLTDFGFCAQI---TPEQSKR----STMVGTPYWMAPEVVTRKAYGPKVD 425
Query: 327 IWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIH-YNRNLIPGYFHDFLDRCLRVPH 503
IWSL + +EM + PY N LRA + I N + F DFL+RCL +
Sbjct: 426 IWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDV 485
Query: 504 WERPSAKQCIQ 536
+R SAK+ +Q
Sbjct: 486 EKRGSAKELLQ 496
>sp|Q8CIN4|PAK2_MOUSE Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2)
Length = 524
Score = 67.0 bits (162), Expect = 4e-11
Identities = 45/131 (34%), Positives = 66/131 (50%), Gaps = 1/131 (0%)
Frame = +3
Query: 147 SVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLKSD 326
+VL+ E ++KLT+F + T +Q S + + W+APEV+ YG K D
Sbjct: 373 NVLLGMEGSVKLTDFGFCAQI---TPEQSKR----STMVGTPYWMAPEVVTRKAYGPKVD 425
Query: 327 IWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIH-YNRNLIPGYFHDFLDRCLRVPH 503
IWSL + +EM + PY N LRA + I N + F DFL+RCL +
Sbjct: 426 IWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDV 485
Query: 504 WERPSAKQCIQ 536
+R SAK+ +Q
Sbjct: 486 EKRGSAKELLQ 496
>sp|Q99759|M3K3_HUMAN Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 67.0 bits (162), Expect = 4e-11
Identities = 42/131 (32%), Positives = 68/131 (51%)
Frame = +3
Query: 141 GSSVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLK 320
G+++L D+ N+KL +F SK L M G + + W++PEV+ + YG K
Sbjct: 492 GANILRDSAGNVKLGDFGASKRLQTICMS----GTGMRSVTGTPYWMSPEVISGEGYGRK 547
Query: 321 SDIWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIHYNRNLIPGYFHDFLDRCLRVP 500
+D+WSL TV+EM T P+ E + A +I + + I + DFL R + V
Sbjct: 548 ADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRR-IFVE 606
Query: 501 HWERPSAKQCI 533
+RPSA++ +
Sbjct: 607 ARQRPSAEELL 617
>sp|Q13177|PAK2_HUMAN Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (PAK65) (Gamma-PAK) (S6/H4 kinase)
Length = 524
Score = 67.0 bits (162), Expect = 4e-11
Identities = 45/131 (34%), Positives = 66/131 (50%), Gaps = 1/131 (0%)
Frame = +3
Query: 147 SVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLKSD 326
+VL+ E ++KLT+F + T +Q S + + W+APEV+ YG K D
Sbjct: 373 NVLLGMEGSVKLTDFGFCAQI---TPEQSKR----STMVGTPYWMAPEVVTRKAYGPKVD 425
Query: 327 IWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIH-YNRNLIPGYFHDFLDRCLRVPH 503
IWSL + +EM + PY N LRA + I N + F DFL+RCL +
Sbjct: 426 IWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDV 485
Query: 504 WERPSAKQCIQ 536
+R SAK+ +Q
Sbjct: 486 EKRGSAKELLQ 496
>sp|Q29502|PAK2_RABIT Serine/threonine-protein kinase PAK 2 (p21-activated kinase 2)
(PAK-2) (Gamma-PAK) (p21-activated protein kinase I)
(PAKI)
Length = 524
Score = 67.0 bits (162), Expect = 4e-11
Identities = 45/131 (34%), Positives = 66/131 (50%), Gaps = 1/131 (0%)
Frame = +3
Query: 147 SVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLKSD 326
+VL+ E ++KLT+F + T +Q S + + W+APEV+ YG K D
Sbjct: 373 NVLLGMEGSVKLTDFGFCAQI---TPEQSKR----STMVGTPYWMAPEVVTRKAYGPKVD 425
Query: 327 IWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIH-YNRNLIPGYFHDFLDRCLRVPH 503
IWSL + +EM + PY N LRA + I N + F DFL+RCL +
Sbjct: 426 IWSLGIMAIEMVEGEPPYLNENPLRALYLIATNGTPELQNPEKLSPIFRDFLNRCLEMDV 485
Query: 504 WERPSAKQCIQ 536
+R SAK+ +Q
Sbjct: 486 EKRGSAKELLQ 496
>sp|Q61084|M3K3_MOUSE Mitogen-activated protein kinase kinase kinase 3 (MAPK/ERK kinase
kinase 3) (MEK kinase 3) (MEKK 3)
Length = 626
Score = 66.6 bits (161), Expect = 5e-11
Identities = 42/131 (32%), Positives = 68/131 (51%)
Frame = +3
Query: 141 GSSVLVDNEDNIKLTNFHLSKYLFNATMDQLLEGIPGSRIETSKCWLAPEVLRNDPYGLK 320
G+++L D+ N+KL +F SK L M G + + W++PEV+ + YG K
Sbjct: 492 GANILRDSAGNVKLGDFGASKRLQTICMS----GTGIRSVTGTPYWMSPEVISGEGYGRK 547
Query: 321 SDIWSLAVTVLEMFTHDRPYPEMNVLRATHRIVYENAIHYNRNLIPGYFHDFLDRCLRVP 500
+D+WSL TV+EM T P+ E + A +I + + I + DFL R + V
Sbjct: 548 ADVWSLGCTVVEMLTEKPPWAEYEAMAAIFKIATQPTNPQLPSHISEHGRDFLRR-IFVE 606
Query: 501 HWERPSAKQCI 533
+RPSA++ +
Sbjct: 607 ARQRPSAEELL 617
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 72,002,468
Number of Sequences: 369166
Number of extensions: 1456758
Number of successful extensions: 5396
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4592
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5234
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5462583840
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)