Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_01532
(747 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinas... 117 4e-26
sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (S... 115 2e-25
sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (... 114 2e-25
sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (... 114 2e-25
sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24 114 4e-25
sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinas... 113 6e-25
sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (S... 112 1e-24
sp|O94804|STK10_HUMAN Serine/threonine-protein kinase 10 (L... 110 4e-24
sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (S... 110 4e-24
sp|P27636|CDC15_YEAST Cell division control protein 15 109 7e-24
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
NPK1-related protein kinase 2)
Length = 651
Score = 117 bits (292), Expect = 4e-26
Identities = 67/169 (39%), Positives = 94/169 (55%), Gaps = 1/169 (0%)
Frame = +1
Query: 55 INKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSL 234
+ K G + Y+ Q+L+GL++LH+ AI+HRDIK NIL+D +KL+DFG S+ +
Sbjct: 162 LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQV 221
Query: 235 QKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGKIPYHNLRPE 414
+ + A + GTP ++APE+ L + + ADIWS G T I M+TGK P+ E
Sbjct: 222 AEL--ATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKE 279
Query: 415 -AVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNH 558
A +F IGTTK P IS F+ L E + RP A LL H
Sbjct: 280 IAAIFHIGTTKSHPPIP-DNISSDANDFLLKCLQQEPNLRPTASELLKH 327
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
(Mammalian STE20-like protein kinase 3)
Length = 443
Score = 115 bits (287), Expect = 2e-25
Identities = 74/199 (37%), Positives = 106/199 (53%), Gaps = 4/199 (2%)
Frame = +1
Query: 28 FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKL 207
+LG S + + + G ++ + ++IL GL +LHS+ IHRDIK N+LL VKL
Sbjct: 113 YLGGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKL 172
Query: 208 SDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGK 387
+DFG++ L T K +TF GTP ++APE+ YD ADIWS G T I + G+
Sbjct: 173 ADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGE 228
Query: 388 IPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNHS 561
P+ L P V+F I +E +P+ E F+ LN E S RP A LL H
Sbjct: 229 PPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKE----FVEACLNKEPSFRPTAKELLKHK 284
Query: 562 -IFKNLR*ILYVV-VIDQY 612
I +N + Y+ +ID+Y
Sbjct: 285 FILRNAKKTSYLTELIDRY 303
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
(Serine/threonine-protein kinase MASK) (Mst3 and
SOK1-related kinase)
Length = 416
Score = 114 bits (286), Expect = 2e-25
Identities = 77/205 (37%), Positives = 110/205 (53%), Gaps = 4/205 (1%)
Frame = +1
Query: 10 SYLW--STFLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILL 183
S LW +LG S + + +AG + + K+IL GL +LHS+ IHRDIK N+LL
Sbjct: 93 SKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKKIHRDIKAANVLL 152
Query: 184 DRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGAT 363
VKL+DFG++ L T K +TF GTP ++APE+ YD ADIWS G T
Sbjct: 153 SEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGIT 208
Query: 364 FIHMITGKIPYHNLRPEAVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRAD 543
I + G+ P ++ P V+F I K T +++ + FI+ LN + S RP A
Sbjct: 209 AIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLVGDFTKSFKEFIDACLNKDPSFRPTAK 266
Query: 544 FLLNHS-IFKNLR*ILYVV-VIDQY 612
LL H I KN + Y+ +ID++
Sbjct: 267 ELLKHKFIVKNSKKTSYLTELIDRF 291
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
(MST-4) (Mammalian STE20-like protein kinase 4)
Length = 416
Score = 114 bits (286), Expect = 2e-25
Identities = 77/205 (37%), Positives = 110/205 (53%), Gaps = 4/205 (1%)
Frame = +1
Query: 10 SYLW--STFLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILL 183
S LW +LG S + + +AG + + K+IL GL +LHS+ IHRDIK N+LL
Sbjct: 93 SKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKKIHRDIKAANVLL 152
Query: 184 DRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGAT 363
VKL+DFG++ L T K +TF GTP ++APE+ YD ADIWS G T
Sbjct: 153 SEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGIT 208
Query: 364 FIHMITGKIPYHNLRPEAVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRAD 543
I + G+ P ++ P V+F I K T +++ + FI+ LN + S RP A
Sbjct: 209 AIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLIGDFTKSFKEFIDACLNKDPSFRPTAK 266
Query: 544 FLLNHS-IFKNLR*ILYVV-VIDQY 612
LL H I KN + Y+ +ID++
Sbjct: 267 ELLKHKFIVKNSKKTSYLTELIDRF 291
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
Length = 431
Score = 114 bits (284), Expect = 4e-25
Identities = 74/199 (37%), Positives = 106/199 (53%), Gaps = 4/199 (2%)
Frame = +1
Query: 28 FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKL 207
+LG S + + + G ++ + ++IL GL +LHS+ IHRDIK N+LL VKL
Sbjct: 101 YLGGGSALDLLEPGPLDEIQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKL 160
Query: 208 SDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGK 387
+DFG++ L T K +TF GTP ++APE+ YD ADIWS G T I + G+
Sbjct: 161 ADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGE 216
Query: 388 IPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNHS 561
P+ L P V+F I +E +P+ E F+ LN E S RP A LL H
Sbjct: 217 PPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKE----FVEACLNKEPSFRPTAKELLKHK 272
Query: 562 -IFKNLR*ILYVV-VIDQY 612
I +N + Y+ +ID+Y
Sbjct: 273 FIIRNAKKTSYLTELIDRY 291
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
NPK1-related protein kinase 1)
Length = 666
Score = 113 bits (282), Expect = 6e-25
Identities = 65/169 (38%), Positives = 96/169 (56%), Gaps = 1/169 (0%)
Frame = +1
Query: 55 INKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSL 234
+ K G + Y++Q+L+GL++LH+ AI+HRDIK NIL+D +KL+DFG S+ +
Sbjct: 163 LEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQV 222
Query: 235 QKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGKIPYHNLRPE 414
+ + + A + GTP ++APE+ L + + ADIWS G T I M+TGK P+ E
Sbjct: 223 AELATMT--GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKE 280
Query: 415 -AVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNH 558
A +F IGTTK P +S + F+ L + RP A LL H
Sbjct: 281 VAAIFFIGTTKSHPPIP-DTLSSDAKDFLLKCLQEVPNLRPTASELLKH 328
>sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 112 bits (279), Expect = 1e-24
Identities = 73/192 (38%), Positives = 99/192 (51%), Gaps = 9/192 (4%)
Frame = +1
Query: 10 SYLWST-------FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKP 168
SYL ST +LG S + + K G +E ++IL GL +LHS+ IHRDIK
Sbjct: 84 SYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKA 143
Query: 169 GNILLDRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIW 348
N+LL VKL+DFG++ L T K +TF GTP ++APE+ YD+ ADIW
Sbjct: 144 ANVLLSEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDFKADIW 199
Query: 349 STGATFIHMITGKIPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQ 522
S G T I + G+ P +L P V+F I + +E +P E F+ LN +
Sbjct: 200 SLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKE----FVEACLNKDP 255
Query: 523 SRRPRADFLLNH 558
RP A LL H
Sbjct: 256 RFRPTAKELLKH 267
>sp|O94804|STK10_HUMAN Serine/threonine-protein kinase 10 (Lymphocyte-oriented kinase)
Length = 968
Score = 110 bits (275), Expect = 4e-24
Identities = 61/163 (37%), Positives = 87/163 (53%), Gaps = 5/163 (3%)
Frame = +1
Query: 103 KQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSLQKTFSQSFDKADTFCG 282
+Q+L L FLHSK IIHRD+K GN+L+ + ++L+DFG+S KT K D+F G
Sbjct: 139 RQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKT----LQKRDSFIG 194
Query: 283 TPNYLAPEIFLGD-----PYDYPADIWSTGATFIHMITGKIPYHNLRPEAVMFRIGTTKK 447
TP ++APE+ + + PYDY ADIWS G T I M + P+H L P V+ +I +
Sbjct: 195 TPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDP 254
Query: 448 IEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNHSIFKNL 576
S + F+ L+ RP A LL H ++
Sbjct: 255 PTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFVSSI 297
>sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (Sterile 20/oxidant
stress-response kinase 1) (Ste20/oxidant stress response
kinase-1) (SOK-1) (Ste20-like kinase)
Length = 426
Score = 110 bits (275), Expect = 4e-24
Identities = 72/192 (37%), Positives = 98/192 (51%), Gaps = 9/192 (4%)
Frame = +1
Query: 10 SYLWST-------FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKP 168
SYL ST +LG S + + K G +E ++IL GL +LHS+ IHRDIK
Sbjct: 84 SYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKA 143
Query: 169 GNILLDRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIW 348
N+LL VK++DFG++ L T K +TF GTP ++APE+ YD+ ADIW
Sbjct: 144 ANVLLSEQGDVKMADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDFKADIW 199
Query: 349 STGATFIHMITGKIPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQ 522
S G T I + G+ P +L P V+F I +E +P E F+ LN +
Sbjct: 200 SLGITAIELAKGEPPNSDLHPMRVLFLIPKNNPPTLEGHHSKPFKE----FVEACLNKDP 255
Query: 523 SRRPRADFLLNH 558
RP A LL H
Sbjct: 256 RFRPTAKELLKH 267
>sp|P27636|CDC15_YEAST Cell division control protein 15
Length = 974
Score = 109 bits (273), Expect = 7e-24
Identities = 61/162 (37%), Positives = 88/162 (54%)
Frame = +1
Query: 73 IETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSLQKTFSQ 252
+ N++ Y Q L+GL++LH + +IHRDIK NILL D TVKL+DFG+S + +
Sbjct: 118 LSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSS--- 174
Query: 253 SFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGKIPYHNLRPEAVMFRI 432
A T GT N++APEI +DIWS GAT + M+T PYHNL + + +
Sbjct: 175 ----ALTLAGTLNWMAPEILGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAV 230
Query: 433 GTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNH 558
+ + P SE ++ F++ +RP AD LL H
Sbjct: 231 ---ENDTYYPPSSFSEPLKDFLSKCFVKNMYKRPTADQLLKH 269
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,667,770
Number of Sequences: 369166
Number of extensions: 1524792
Number of successful extensions: 7185
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5561
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)