Planaria EST Database


DrC_01532

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_01532
         (747 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9FZ36|M3K2_ARATH  Mitogen-activated protein kinase kinas...   117   4e-26
sp|Q9Y6E0|STK24_HUMAN  Serine/threonine-protein kinase 24 (S...   115   2e-25
sp|Q9P289|MST4_HUMAN  Serine/threonine-protein kinase MST4 (...   114   2e-25
sp|Q99JT2|MST4_MOUSE  Serine/threonine-protein kinase MST4 (...   114   2e-25
sp|Q99KH8|STK24_MOUSE  Serine/threonine-protein kinase 24         114   4e-25
sp|O22040|M3K1_ARATH  Mitogen-activated protein kinase kinas...   113   6e-25
sp|O00506|STK25_HUMAN  Serine/threonine-protein kinase 25 (S...   112   1e-24
sp|O94804|STK10_HUMAN  Serine/threonine-protein kinase 10 (L...   110   4e-24
sp|Q9Z2W1|STK25_MOUSE  Serine/threonine-protein kinase 25 (S...   110   4e-24
sp|P27636|CDC15_YEAST  Cell division control protein 15           109   7e-24
>sp|Q9FZ36|M3K2_ARATH Mitogen-activated protein kinase kinase kinase 2 (Arabidospsis
           NPK1-related protein kinase 2)
          Length = 651

 Score =  117 bits (292), Expect = 4e-26
 Identities = 67/169 (39%), Positives = 94/169 (55%), Gaps = 1/169 (0%)
 Frame = +1

Query: 55  INKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSL 234
           + K G    +    Y+ Q+L+GL++LH+ AI+HRDIK  NIL+D    +KL+DFG S+ +
Sbjct: 162 LEKFGAFPESVVRTYTNQLLLGLEYLHNHAIMHRDIKGANILVDNQGCIKLADFGASKQV 221

Query: 235 QKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGKIPYHNLRPE 414
            +    +   A +  GTP ++APE+ L   + + ADIWS G T I M+TGK P+     E
Sbjct: 222 AEL--ATISGAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKE 279

Query: 415 -AVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNH 558
            A +F IGTTK     P   IS     F+   L  E + RP A  LL H
Sbjct: 280 IAAIFHIGTTKSHPPIP-DNISSDANDFLLKCLQQEPNLRPTASELLKH 327
>sp|Q9Y6E0|STK24_HUMAN Serine/threonine-protein kinase 24 (STE20-like kinase MST3) (MST-3)
           (Mammalian STE20-like protein kinase 3)
          Length = 443

 Score =  115 bits (287), Expect = 2e-25
 Identities = 74/199 (37%), Positives = 106/199 (53%), Gaps = 4/199 (2%)
 Frame = +1

Query: 28  FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKL 207
           +LG  S + + + G ++  +     ++IL GL +LHS+  IHRDIK  N+LL     VKL
Sbjct: 113 YLGGGSALDLLEPGPLDETQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKL 172

Query: 208 SDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGK 387
           +DFG++  L  T      K +TF GTP ++APE+     YD  ADIWS G T I +  G+
Sbjct: 173 ADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELARGE 228

Query: 388 IPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNHS 561
            P+  L P  V+F I       +E    +P+ E    F+   LN E S RP A  LL H 
Sbjct: 229 PPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKE----FVEACLNKEPSFRPTAKELLKHK 284

Query: 562 -IFKNLR*ILYVV-VIDQY 612
            I +N +   Y+  +ID+Y
Sbjct: 285 FILRNAKKTSYLTELIDRY 303
>sp|Q9P289|MST4_HUMAN Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
           (MST-4) (Mammalian STE20-like protein kinase 4)
           (Serine/threonine-protein kinase MASK) (Mst3 and
           SOK1-related kinase)
          Length = 416

 Score =  114 bits (286), Expect = 2e-25
 Identities = 77/205 (37%), Positives = 110/205 (53%), Gaps = 4/205 (1%)
 Frame = +1

Query: 10  SYLW--STFLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILL 183
           S LW    +LG  S + + +AG  +  +     K+IL GL +LHS+  IHRDIK  N+LL
Sbjct: 93  SKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKKIHRDIKAANVLL 152

Query: 184 DRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGAT 363
                VKL+DFG++  L  T      K +TF GTP ++APE+     YD  ADIWS G T
Sbjct: 153 SEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGIT 208

Query: 364 FIHMITGKIPYHNLRPEAVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRAD 543
            I +  G+ P  ++ P  V+F I   K    T     +++ + FI+  LN + S RP A 
Sbjct: 209 AIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLVGDFTKSFKEFIDACLNKDPSFRPTAK 266

Query: 544 FLLNHS-IFKNLR*ILYVV-VIDQY 612
            LL H  I KN +   Y+  +ID++
Sbjct: 267 ELLKHKFIVKNSKKTSYLTELIDRF 291
>sp|Q99JT2|MST4_MOUSE Serine/threonine-protein kinase MST4 (STE20-like kinase MST4)
           (MST-4) (Mammalian STE20-like protein kinase 4)
          Length = 416

 Score =  114 bits (286), Expect = 2e-25
 Identities = 77/205 (37%), Positives = 110/205 (53%), Gaps = 4/205 (1%)
 Frame = +1

Query: 10  SYLW--STFLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILL 183
           S LW    +LG  S + + +AG  +  +     K+IL GL +LHS+  IHRDIK  N+LL
Sbjct: 93  SKLWIIMEYLGGGSALDLLRAGPFDEFQIATMLKEILKGLDYLHSEKKIHRDIKAANVLL 152

Query: 184 DRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGAT 363
                VKL+DFG++  L  T      K +TF GTP ++APE+     YD  ADIWS G T
Sbjct: 153 SEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIQQSAYDSKADIWSLGIT 208

Query: 364 FIHMITGKIPYHNLRPEAVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRAD 543
            I +  G+ P  ++ P  V+F I   K    T     +++ + FI+  LN + S RP A 
Sbjct: 209 AIELAKGEPPNSDMHPMRVLFLI--PKNNPPTLIGDFTKSFKEFIDACLNKDPSFRPTAK 266

Query: 544 FLLNHS-IFKNLR*ILYVV-VIDQY 612
            LL H  I KN +   Y+  +ID++
Sbjct: 267 ELLKHKFIVKNSKKTSYLTELIDRF 291
>sp|Q99KH8|STK24_MOUSE Serine/threonine-protein kinase 24
          Length = 431

 Score =  114 bits (284), Expect = 4e-25
 Identities = 74/199 (37%), Positives = 106/199 (53%), Gaps = 4/199 (2%)
 Frame = +1

Query: 28  FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKL 207
           +LG  S + + + G ++  +     ++IL GL +LHS+  IHRDIK  N+LL     VKL
Sbjct: 101 YLGGGSALDLLEPGPLDEIQIATILREILKGLDYLHSEKKIHRDIKAANVLLSEHGEVKL 160

Query: 208 SDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGK 387
           +DFG++  L  T      K +TF GTP ++APE+     YD  ADIWS G T I +  G+
Sbjct: 161 ADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDSKADIWSLGITAIELAKGE 216

Query: 388 IPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNHS 561
            P+  L P  V+F I       +E    +P+ E    F+   LN E S RP A  LL H 
Sbjct: 217 PPHSELHPMKVLFLIPKNNPPTLEGNYSKPLKE----FVEACLNKEPSFRPTAKELLKHK 272

Query: 562 -IFKNLR*ILYVV-VIDQY 612
            I +N +   Y+  +ID+Y
Sbjct: 273 FIIRNAKKTSYLTELIDRY 291
>sp|O22040|M3K1_ARATH Mitogen-activated protein kinase kinase kinase 1 (Arabidospsis
           NPK1-related protein kinase 1)
          Length = 666

 Score =  113 bits (282), Expect = 6e-25
 Identities = 65/169 (38%), Positives = 96/169 (56%), Gaps = 1/169 (0%)
 Frame = +1

Query: 55  INKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSL 234
           + K G    +    Y++Q+L+GL++LH+ AI+HRDIK  NIL+D    +KL+DFG S+ +
Sbjct: 163 LEKFGPFPESVVRTYTRQLLLGLEYLHNHAIMHRDIKGANILVDNKGCIKLADFGASKQV 222

Query: 235 QKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGKIPYHNLRPE 414
            +  + +   A +  GTP ++APE+ L   + + ADIWS G T I M+TGK P+     E
Sbjct: 223 AELATMT--GAKSMKGTPYWMAPEVILQTGHSFSADIWSVGCTVIEMVTGKAPWSQQYKE 280

Query: 415 -AVMFRIGTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNH 558
            A +F IGTTK     P   +S   + F+   L    + RP A  LL H
Sbjct: 281 VAAIFFIGTTKSHPPIP-DTLSSDAKDFLLKCLQEVPNLRPTASELLKH 328
>sp|O00506|STK25_HUMAN Serine/threonine-protein kinase 25 (Sterile 20/oxidant
           stress-response kinase 1) (Ste20/oxidant stress response
           kinase-1) (SOK-1) (Ste20-like kinase)
          Length = 426

 Score =  112 bits (279), Expect = 1e-24
 Identities = 73/192 (38%), Positives = 99/192 (51%), Gaps = 9/192 (4%)
 Frame = +1

Query: 10  SYLWST-------FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKP 168
           SYL ST       +LG  S + + K G +E        ++IL GL +LHS+  IHRDIK 
Sbjct: 84  SYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKA 143

Query: 169 GNILLDRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIW 348
            N+LL     VKL+DFG++  L  T      K +TF GTP ++APE+     YD+ ADIW
Sbjct: 144 ANVLLSEQGDVKLADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDFKADIW 199

Query: 349 STGATFIHMITGKIPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQ 522
           S G T I +  G+ P  +L P  V+F I   +   +E    +P  E    F+   LN + 
Sbjct: 200 SLGITAIELAKGEPPNSDLHPMRVLFLIPKNSPPTLEGQHSKPFKE----FVEACLNKDP 255

Query: 523 SRRPRADFLLNH 558
             RP A  LL H
Sbjct: 256 RFRPTAKELLKH 267
>sp|O94804|STK10_HUMAN Serine/threonine-protein kinase 10 (Lymphocyte-oriented kinase)
          Length = 968

 Score =  110 bits (275), Expect = 4e-24
 Identities = 61/163 (37%), Positives = 87/163 (53%), Gaps = 5/163 (3%)
 Frame = +1

Query: 103 KQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSLQKTFSQSFDKADTFCG 282
           +Q+L  L FLHSK IIHRD+K GN+L+  +  ++L+DFG+S    KT      K D+F G
Sbjct: 139 RQMLEALNFLHSKRIIHRDLKAGNVLMTLEGDIRLADFGVSAKNLKT----LQKRDSFIG 194

Query: 283 TPNYLAPEIFLGD-----PYDYPADIWSTGATFIHMITGKIPYHNLRPEAVMFRIGTTKK 447
           TP ++APE+ + +     PYDY ADIWS G T I M   + P+H L P  V+ +I  +  
Sbjct: 195 TPYWMAPEVVMCETMKDTPYDYKADIWSLGITLIEMAQIEPPHHELNPMRVLLKIAKSDP 254

Query: 448 IEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNHSIFKNL 576
                    S   + F+   L+     RP A  LL H    ++
Sbjct: 255 PTLLTPSKWSVEFRDFLKIALDKNPETRPSAAQLLEHPFVSSI 297
>sp|Q9Z2W1|STK25_MOUSE Serine/threonine-protein kinase 25 (Sterile 20/oxidant
           stress-response kinase 1) (Ste20/oxidant stress response
           kinase-1) (SOK-1) (Ste20-like kinase)
          Length = 426

 Score =  110 bits (275), Expect = 4e-24
 Identities = 72/192 (37%), Positives = 98/192 (51%), Gaps = 9/192 (4%)
 Frame = +1

Query: 10  SYLWST-------FLGAPSKICINKAGMIETNKAIQYSKQILVGLQFLHSKAIIHRDIKP 168
           SYL ST       +LG  S + + K G +E        ++IL GL +LHS+  IHRDIK 
Sbjct: 84  SYLKSTKLWIIMEYLGGGSALDLLKPGPLEETYIATILREILKGLDYLHSERKIHRDIKA 143

Query: 169 GNILLDRDETVKLSDFGISRSLQKTFSQSFDKADTFCGTPNYLAPEIFLGDPYDYPADIW 348
            N+LL     VK++DFG++  L  T      K +TF GTP ++APE+     YD+ ADIW
Sbjct: 144 ANVLLSEQGDVKMADFGVAGQLTDTQI----KRNTFVGTPFWMAPEVIKQSAYDFKADIW 199

Query: 349 STGATFIHMITGKIPYHNLRPEAVMFRI--GTTKKIEFTPCQPISETMQSFINDLLNPEQ 522
           S G T I +  G+ P  +L P  V+F I       +E    +P  E    F+   LN + 
Sbjct: 200 SLGITAIELAKGEPPNSDLHPMRVLFLIPKNNPPTLEGHHSKPFKE----FVEACLNKDP 255

Query: 523 SRRPRADFLLNH 558
             RP A  LL H
Sbjct: 256 RFRPTAKELLKH 267
>sp|P27636|CDC15_YEAST Cell division control protein 15
          Length = 974

 Score =  109 bits (273), Expect = 7e-24
 Identities = 61/162 (37%), Positives = 88/162 (54%)
 Frame = +1

Query: 73  IETNKAIQYSKQILVGLQFLHSKAIIHRDIKPGNILLDRDETVKLSDFGISRSLQKTFSQ 252
           +  N++  Y  Q L+GL++LH + +IHRDIK  NILL  D TVKL+DFG+S  +  +   
Sbjct: 118 LSENESKTYVTQTLLGLKYLHGEGVIHRDIKAANILLSADNTVKLADFGVSTIVNSS--- 174

Query: 253 SFDKADTFCGTPNYLAPEIFLGDPYDYPADIWSTGATFIHMITGKIPYHNLRPEAVMFRI 432
               A T  GT N++APEI         +DIWS GAT + M+T   PYHNL    + + +
Sbjct: 175 ----ALTLAGTLNWMAPEILGNRGASTLSDIWSLGATVVEMLTKNPPYHNLTDANIYYAV 230

Query: 433 GTTKKIEFTPCQPISETMQSFINDLLNPEQSRRPRADFLLNH 558
              +   + P    SE ++ F++        +RP AD LL H
Sbjct: 231 ---ENDTYYPPSSFSEPLKDFLSKCFVKNMYKRPTADQLLKH 269
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,667,770
Number of Sequences: 369166
Number of extensions: 1524792
Number of successful extensions: 7185
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5184
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5561
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6776504000
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)