Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_01256
(804 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O77784|IDH3B_BOVIN Isocitrate dehydrogenase [NAD] subuni... 285 1e-76
sp|Q28479|IDH3B_MACFA Isocitrate dehydrogenase [NAD] subuni... 284 2e-76
sp|Q68FX0|IDH3B_RAT Isocitrate dehydrogenase [NAD] subunit ... 284 2e-76
sp|O43837|IDH3B_HUMAN Isocitrate dehydrogenase [NAD] subuni... 283 3e-76
sp|Q93353|IDH3B_CAEEL Probable isocitrate dehydrogenase [NA... 280 3e-75
sp|Q5RBT4|IDH3B_PONPY Isocitrate dehydrogenase [NAD] subuni... 278 1e-74
sp|P41565|IDH3G_RAT Isocitrate dehydrogenase [NAD] subunit ... 258 1e-68
sp|P70404|IDH3G_MOUSE Isocitrate dehydrogenase [NAD] subuni... 258 1e-68
sp|P41564|IDH3G_MACFA Isocitrate dehydrogenase [NAD] subuni... 257 2e-68
sp|P51553|IDH3G_HUMAN Isocitrate dehydrogenase [NAD] subuni... 257 2e-68
>sp|O77784|IDH3B_BOVIN Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH) (NAD(+)-isocitrate dehydrogenase subunit 1) (IDH1)
Length = 385
Score = 285 bits (729), Expect = 1e-76
Identities = 139/240 (57%), Positives = 185/240 (77%), Gaps = 2/240 (0%)
Frame = +2
Query: 89 ITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDS-VNDVVESFQRNKVG 265
+T+LPGDGV P+L+ +K +F+ +V + + +LSE+ + S+ + V+ S + NKV
Sbjct: 51 VTMLPGDGVGPELMHAVKEVFKAASVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVA 110
Query: 266 IKGIIATPL-HGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQTEGE 442
I G I TP+ + +L + +M+ R +LDLFANV+ V+S G TRH N+D V+IREQTEGE
Sbjct: 111 IIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIREQTEGE 170
Query: 443 YSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGDGLFL 622
YS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAVHKANIMKLGDGLFL
Sbjct: 171 YSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFL 230
Query: 623 ESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIGGAGL 802
+ C V++LYP IK + +I+DNCCMQLV NP QFDVLV PNLYG ++DN+AAGL+GGAG+
Sbjct: 231 QCCEEVAELYPKIKFEKMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGV 290
>sp|Q28479|IDH3B_MACFA Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 381
Score = 284 bits (727), Expect = 2e-76
Identities = 139/240 (57%), Positives = 184/240 (76%), Gaps = 2/240 (0%)
Frame = +2
Query: 89 ITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDS-VNDVVESFQRNKVG 265
+T+LPGDGV P+L+ +K +F+ V + + +LSE+ + S+ + V+ S + NKV
Sbjct: 49 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVA 108
Query: 266 IKGIIATPL-HGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQTEGE 442
I G I TP+ + +L + +M+ R +LDLFANV+ V+S G TRH N+D V+IREQTEGE
Sbjct: 109 IIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGE 168
Query: 443 YSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGDGLFL 622
YS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAVHKANIMKLGDGLFL
Sbjct: 169 YSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRSKVTAVHKANIMKLGDGLFL 228
Query: 623 ESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIGGAGL 802
+ C V++LYP IK + +I+DNCCMQLV NP QFDVLV PNLYG ++DN+AAGL+GGAG+
Sbjct: 229 QCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGV 288
>sp|Q68FX0|IDH3B_RAT Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 385
Score = 284 bits (726), Expect = 2e-76
Identities = 139/240 (57%), Positives = 184/240 (76%), Gaps = 2/240 (0%)
Frame = +2
Query: 89 ITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDS-VNDVVESFQRNKVG 265
+T+LPGDGV P+L+ +K +F+ V + + +LSE+ + S+ + V+ S + NKV
Sbjct: 51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFKEHHLSEVQNMASEEKLEQVLSSMKENKVA 110
Query: 266 IKGIIATPL-HGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQTEGE 442
I G I TP+ + +L + +M+ R +LDLFANV+ V+S G TRH N+D V+IREQTEGE
Sbjct: 111 IIGKIYTPMEYKGELASYDMQLRRKLDLFANVVHVKSLPGYKTRHNNLDLVIIREQTEGE 170
Query: 443 YSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGDGLFL 622
YS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAVHKANIMKLGDGLFL
Sbjct: 171 YSSLEHESARGVIECLKIVTRTKSQRIAKFAFDYATKKGRSKVTAVHKANIMKLGDGLFL 230
Query: 623 ESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIGGAGL 802
+ C V++LYP IK + +I+DNCCMQLV NP QFDVLV PNLYG ++DN+AAGL+GGAG+
Sbjct: 231 QCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGV 290
>sp|O43837|IDH3B_HUMAN Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 385
Score = 283 bits (725), Expect = 3e-76
Identities = 139/240 (57%), Positives = 184/240 (76%), Gaps = 2/240 (0%)
Frame = +2
Query: 89 ITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDS-VNDVVESFQRNKVG 265
+T+LPGDGV P+L+ +K +F+ V + + +LSE+ + S+ + V+ S + NKV
Sbjct: 51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVA 110
Query: 266 IKGIIATPL-HGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQTEGE 442
I G I TP+ + +L + +M+ R +LDLFANV+ V+S G TRH N+D V+IREQTEGE
Sbjct: 111 IIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGE 170
Query: 443 YSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGDGLFL 622
YS+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KVTAVHKANIMKLGDGLFL
Sbjct: 171 YSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRGKVTAVHKANIMKLGDGLFL 230
Query: 623 ESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIGGAGL 802
+ C V++LYP IK + +I+DNCCMQLV NP QFDVLV PNLYG ++DN+AAGL+GGAG+
Sbjct: 231 QCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGV 290
>sp|Q93353|IDH3B_CAEEL Probable isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 379
Score = 280 bits (716), Expect = 3e-75
Identities = 140/248 (56%), Positives = 188/248 (75%), Gaps = 5/248 (2%)
Frame = +2
Query: 74 HQNKTITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDSVNDVVESFQR 253
+Q +T++PGDGV P+L+ ++ I + + + E +LSE+ +S S+ + VES R
Sbjct: 40 NQKLKVTIIPGDGVGPELIYTVQDIVKQTGIPIEFEEIFLSEVHYTRSSSIENAVESIGR 99
Query: 254 NK-VGIKGII--ATPLHG-AQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRH-KNIDFVV 418
N V +KG I + LH +LQ +NM+ R LDLFANV+ +++ DG+ TRH K +DFV+
Sbjct: 100 NNNVALKGAIEESAVLHTEGELQGLNMRLRRSLDLFANVVHIKTLDGIKTRHGKQLDFVI 159
Query: 419 IREQTEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIM 598
+REQTEGEYS+LEHE VPGV+E LKI T++K+ERIAKFAFD+A K GRKKVTAVHKANIM
Sbjct: 160 VREQTEGEYSSLEHELVPGVIECLKISTRTKAERIAKFAFDYATKTGRKKVTAVHKANIM 219
Query: 599 KLGDGLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAA 778
KLGDGLFL +C V+K YP I+ + +I+DN CMQLVS P+QFDV+V PNLYG ++DN+AA
Sbjct: 220 KLGDGLFLRTCEGVAKQYPKIQFESMIIDNTCMQLVSKPEQFDVMVMPNLYGNIIDNLAA 279
Query: 779 GLIGGAGL 802
GL+GGAG+
Sbjct: 280 GLVGGAGV 287
>sp|Q5RBT4|IDH3B_PONPY Isocitrate dehydrogenase [NAD] subunit beta, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 385
Score = 278 bits (712), Expect = 1e-74
Identities = 137/240 (57%), Positives = 182/240 (75%), Gaps = 2/240 (0%)
Frame = +2
Query: 89 ITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDS-VNDVVESFQRNKVG 265
+T+LPGDGV P+L+ +K +F+ V + + +LSE+ + S+ + V+ S + NKV
Sbjct: 51 VTMLPGDGVGPELMHAVKEVFKAAAVPVEFQEHHLSEVQNMASEEKLEQVLSSMKENKVA 110
Query: 266 IKGIIATPL-HGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQTEGE 442
I G I TP+ + +L + +M+ R +LDLFANV+ V+S G TRH N+D V+IREQTEGE
Sbjct: 111 IIGKIHTPMEYKGELASYDMRLRRKLDLFANVVHVKSLPGYMTRHNNLDLVIIREQTEGE 170
Query: 443 YSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGDGLFL 622
S+LEHES GV+E LKI+T++KS+RIAKFAFD+A K GR KV AVHKANIMKLGDGLFL
Sbjct: 171 CSSLEHESARGVIECLKIVTRAKSQRIAKFAFDYATKKGRSKVIAVHKANIMKLGDGLFL 230
Query: 623 ESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIGGAGL 802
+ C V++LYP IK + +I+DNCCMQLV NP QFDVLV PNLYG ++DN+AAGL+GGAG+
Sbjct: 231 QCCEEVAELYPKIKFETMIIDNCCMQLVQNPYQFDVLVMPNLYGNIIDNLAAGLVGGAGV 290
>sp|P41565|IDH3G_RAT Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 393
Score = 258 bits (660), Expect = 1e-68
Identities = 134/244 (54%), Positives = 176/244 (72%), Gaps = 5/244 (2%)
Frame = +2
Query: 86 TITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDSVNDVVESFQRNKVG 265
T+T++PGDG+ P+L+ H+K +FR V ++ E ++S + + + + + +RN+V
Sbjct: 56 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSS--NADEEDIRNAIMAIRRNRVA 113
Query: 266 IKGIIAT-----PLHGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQ 430
+KG I T P H ++ N R LDL+ANV+ +S GV TRHK+ID +++RE
Sbjct: 114 LKGNIETNHDLPPSHKSR----NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVREN 169
Query: 431 TEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGD 610
TEGEYS+LEHESV GVVESLKIITK+KS RIA++AF A++ GRKKVTAVHKANIMKLGD
Sbjct: 170 TEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGD 229
Query: 611 GLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIG 790
GLFL+ CR V+ YP I D +IVDN MQLVS PQQFDV+V PNLYG +V+NV AGL+G
Sbjct: 230 GLFLQCCREVAARYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Query: 791 GAGL 802
G GL
Sbjct: 290 GPGL 293
>sp|P70404|IDH3G_MOUSE Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 393
Score = 258 bits (659), Expect = 1e-68
Identities = 134/244 (54%), Positives = 176/244 (72%), Gaps = 5/244 (2%)
Frame = +2
Query: 86 TITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDSVNDVVESFQRNKVG 265
T+T++PGDG+ P+L+ H+K +FR V ++ E ++S + + + + + +RN+V
Sbjct: 56 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSS--NADEEDIRNAIMAIRRNRVA 113
Query: 266 IKGIIAT-----PLHGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQ 430
+KG I T P H ++ N R LDL+ANV+ +S GV TRHK+ID +++RE
Sbjct: 114 LKGNIETNHNLPPSHKSR----NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVREN 169
Query: 431 TEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGD 610
TEGEYS+LEHESV GVVESLKIITK+KS RIA++AF A++ GRKKVTAVHKANIMKLGD
Sbjct: 170 TEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGD 229
Query: 611 GLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIG 790
GLFL+ CR V+ YP I D +IVDN MQLVS PQQFDV+V PNLYG +V+NV AGL+G
Sbjct: 230 GLFLQCCREVAAHYPQITFDSMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Query: 791 GAGL 802
G GL
Sbjct: 290 GPGL 293
>sp|P41564|IDH3G_MACFA Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 355
Score = 257 bits (657), Expect = 2e-68
Identities = 133/244 (54%), Positives = 177/244 (72%), Gaps = 5/244 (2%)
Frame = +2
Query: 86 TITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDSVNDVVESFQRNKVG 265
T+T++PGDG+ P+L+ H+K +FR V ++ E ++S + + + + + +RN+V
Sbjct: 18 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSS--NADEEDIRNAIMAIRRNRVA 75
Query: 266 IKGIIAT-----PLHGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQ 430
+KG I T P H ++ N R LDL+ANV+ +S GV TRHK+ID +++RE
Sbjct: 76 LKGNIETNHNLPPSHKSR----NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVREN 131
Query: 431 TEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGD 610
TEGEYS+LEHESV GVVESLKIITK+KS RIA++AF A++ GRKKVTAVHKANIMKLGD
Sbjct: 132 TEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGD 191
Query: 611 GLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIG 790
GLFL+ CR V+ YP I +++IVDN MQLVS PQQFDV+V PNLYG +V+NV AGL+G
Sbjct: 192 GLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 251
Query: 791 GAGL 802
G GL
Sbjct: 252 GPGL 255
>sp|P51553|IDH3G_HUMAN Isocitrate dehydrogenase [NAD] subunit gamma, mitochondrial
precursor (Isocitric dehydrogenase) (NAD(+)-specific
ICDH)
Length = 393
Score = 257 bits (657), Expect = 2e-68
Identities = 133/244 (54%), Positives = 177/244 (72%), Gaps = 5/244 (2%)
Frame = +2
Query: 86 TITVLPGDGVWPDLVEHLKHIFRIMNVKLNLESKYLSELPGVKSDSVNDVVESFQRNKVG 265
T+T++PGDG+ P+L+ H+K +FR V ++ E ++S + + + + + +RN+V
Sbjct: 56 TVTMIPGDGIGPELMLHVKSVFRHACVPVDFEEVHVSS--NADEEDIRNAIMAIRRNRVA 113
Query: 266 IKGIIAT-----PLHGAQLQTVNMKFRGQLDLFANVMIVRSFDGVPTRHKNIDFVVIREQ 430
+KG I T P H ++ N R LDL+ANV+ +S GV TRHK+ID +++RE
Sbjct: 114 LKGNIETNHNLPPSHKSR----NNILRTSLDLYANVIHCKSLPGVVTRHKDIDILIVREN 169
Query: 431 TEGEYSALEHESVPGVVESLKIITKSKSERIAKFAFDFAKKHGRKKVTAVHKANIMKLGD 610
TEGEYS+LEHESV GVVESLKIITK+KS RIA++AF A++ GRKKVTAVHKANIMKLGD
Sbjct: 170 TEGEYSSLEHESVAGVVESLKIITKAKSLRIAEYAFKLAQESGRKKVTAVHKANIMKLGD 229
Query: 611 GLFLESCRNVSKLYPDIKLDDIIVDNCCMQLVSNPQQFDVLVTPNLYGAVVDNVAAGLIG 790
GLFL+ CR V+ YP I +++IVDN MQLVS PQQFDV+V PNLYG +V+NV AGL+G
Sbjct: 230 GLFLQCCREVAARYPQITFENMIVDNTTMQLVSRPQQFDVMVMPNLYGNIVNNVCAGLVG 289
Query: 791 GAGL 802
G GL
Sbjct: 290 GPGL 293
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 92,917,808
Number of Sequences: 369166
Number of extensions: 1877184
Number of successful extensions: 6250
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5762
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5973
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)