Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= DrC_01166
(745 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P13707|GPDA_MOUSE Glycerol-3-phosphate dehydrogenase [NA... 262 8e-70
sp|Q27928|GPDA_DROPS Glycerol-3-phosphate dehydrogenase [NA... 262 8e-70
sp|Q27567|GPDA_DROEZ Glycerol-3-phosphate dehydrogenase [NA... 260 3e-69
sp|P13706|GPDA_DROME Glycerol-3-phosphate dehydrogenase [NA... 259 4e-69
sp|Q27556|GPDA_DROAE Glycerol-3-phosphate dehydrogenase [NA... 259 4e-69
sp|P07735|GPDA_DROVI Glycerol-3-phosphate dehydrogenase [NA... 259 5e-69
sp|O97463|GPDA_DROKA Glycerol-3-phosphate dehydrogenase [NA... 259 5e-69
sp|P21695|GPDA_HUMAN Glycerol-3-phosphate dehydrogenase [NA... 258 2e-68
sp|O57656|GPDA_FUGRU Glycerol-3-phosphate dehydrogenase [NA... 256 3e-68
sp|O35077|GPDA_RAT Glycerol-3-phosphate dehydrogenase [NAD+... 256 4e-68
>sp|P13707|GPDA_MOUSE Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 349
Score = 262 bits (669), Expect = 8e-70
Identities = 126/220 (57%), Positives = 164/220 (74%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS++I ++L IP + LMGAN+A EV++E +CE TIG DPA+G LK L QT FR+ V
Sbjct: 131 LISEVIGERLGIPMSVLMGANIASEVAEEKFCETTIGCKDPAQGQLLKDLMQTPNFRITV 190
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
+++ E+CGALKNIVA+GAGF +GLG+GDNTKAA+IRLG MEMI FA F ++
Sbjct: 191 VQEVDTVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSGT-VSS 249
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
TFLESCGVADLITTCYGGRN+++ +A TGKS+ QLE E+LNGQ QGP T++E++ +
Sbjct: 250 ATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKEMLNGQKLQGPQTARELHSI 309
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L ++FPLFTA+Y +C P +FI CL+NHPEHM
Sbjct: 310 LQHKGLVDKFPLFTAVYKVCYEGQPVGEFIRCLQNHPEHM 349
>sp|Q27928|GPDA_DROPS Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 353
Score = 262 bits (669), Expect = 8e-70
Identities = 129/220 (58%), Positives = 163/220 (74%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS II L IPC LMGANLA+EV++ +CE TIG +D G L+ LFQ ++FR+VV
Sbjct: 133 LISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVV 192
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
+ D E+CGALKNIVA GAGF +GL GDNTKAA+IRLG MEMI+F + F+ G
Sbjct: 193 VDDADAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFYP--GSKL 250
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
+TF ESCGVADLITTCYGGRN+R+ +A V +GK++ +LE E+LNGQ QGP T++EVN +
Sbjct: 251 STFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEELEKEMLNGQKLQGPPTAEEVNYM 310
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L K E++FPLFTAI+ IC ++ P IDC+RNHPEHM
Sbjct: 311 LKNKKLEDKFPLFTAIHKICTNQLKPKDLIDCIRNHPEHM 350
>sp|Q27567|GPDA_DROEZ Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 350
Score = 260 bits (664), Expect = 3e-69
Identities = 128/220 (58%), Positives = 163/220 (74%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS II L IPC LMGANLA+EV++ +CE TIG +D G L+ LFQ ++FR+VV
Sbjct: 133 LISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVV 192
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
++D E+CGALKNIVA GAGF +GL GDNTKAA+IRLG MEMI+F + F+ G
Sbjct: 193 VEDADAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFYP--GSKL 250
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
+TF ESCGVADLITTCYGGRN+R+ +A V +GK++ +LE E+LNGQ QGP T++EVN +
Sbjct: 251 STFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEELEKEMLNGQKLQGPPTAEEVNYM 310
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L E++FPLFTAI+ IC ++ P IDC+RNHPEHM
Sbjct: 311 LKNKGLEDKFPLFTAIHKICTNQLKPKDLIDCIRNHPEHM 350
>sp|P13706|GPDA_DROME Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 363
Score = 259 bits (663), Expect = 4e-69
Identities = 128/220 (58%), Positives = 163/220 (74%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS II L IPC LMGANLA+EV++ +CE TIG +D G L+ LFQ ++FR+VV
Sbjct: 133 LISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVV 192
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
+ D E+CGALKNIVA GAGF +GL GDNTKAA+IRLG MEMI+F + F+ G
Sbjct: 193 VDDADAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFYP--GSKL 250
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
+TF ESCGVADLITTCYGGRN+R+ +A V +GK++ +LE E+LNGQ QGP T++EVN +
Sbjct: 251 STFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEELEKEMLNGQKLQGPPTAEEVNYM 310
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L E++FPLFTAI+ IC ++ P+ IDC+RNHPEHM
Sbjct: 311 LKNKGLEDKFPLFTAIHKICTNQLKPNDLIDCIRNHPEHM 350
>sp|Q27556|GPDA_DROAE Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 350
Score = 259 bits (663), Expect = 4e-69
Identities = 128/220 (58%), Positives = 162/220 (73%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS II L IPC LMGANLA+EV++ +CE TIG +D G L+ LFQ ++FR+VV
Sbjct: 133 LISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVV 192
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
++D E+CGALKNIVA GAGF +GL GDNTKAA+IRLG MEMI+F + F+ G
Sbjct: 193 VEDAEAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFY--PGSKL 250
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
+TF ESCGVADLITTCYGGRN+R+ +A V +GK++ LE E+LNGQ QGP T++EVN +
Sbjct: 251 STFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEDLEKEMLNGQKLQGPPTAEEVNYM 310
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L E++FPLFTAI+ IC ++ P IDC+RNHPEHM
Sbjct: 311 LKNKGLEDKFPLFTAIHKICTNQLKPKDLIDCIRNHPEHM 350
>sp|P07735|GPDA_DROVI Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 353
Score = 259 bits (662), Expect = 5e-69
Identities = 128/220 (58%), Positives = 162/220 (73%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS II L IPC LMGANLA+EV++ +CE TIG +D G L+ LFQ ++FR+VV
Sbjct: 133 LISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVV 192
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
++D E+CGALKNIVA GAGF +GL GDNTKAA+IRLG MEMI+F + F+ G
Sbjct: 193 VEDADAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFYP--GSKL 250
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
+TF ESCGVADLITTCYGGRN+R+ +A V +GK++ LE E+LNGQ QGP T++EVN +
Sbjct: 251 STFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEDLEKEMLNGQKLQGPPTAEEVNYM 310
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L E++FPLFTAI+ IC ++ P IDC+RNHPEHM
Sbjct: 311 LKNKGLEDKFPLFTAIHKICTNQLKPKDLIDCIRNHPEHM 350
>sp|O97463|GPDA_DROKA Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 360
Score = 259 bits (662), Expect = 5e-69
Identities = 128/220 (58%), Positives = 163/220 (74%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS II L IPC LMGANLA+EV++ +CE TIG +D G L+ LFQ ++FR+VV
Sbjct: 133 LISHIITRHLKIPCAVLMGANLANEVAEGNFCETTIGCTDKKYGKVLRDLFQANHFRVVV 192
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
++D E+CGALKNIVA GAGF +GL GDNTKAA+IRLG MEMI+F + F+ G
Sbjct: 193 VEDADAVEVCGALKNIVACGAGFVDGLKLGDNTKAAVIRLGLMEMIRFVDVFYP--GSKL 250
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
+TF ESCGVADLITTCYGGRN+R+ +A V +GK++ +LE E+LNGQ QGP T++EVN +
Sbjct: 251 STFFESCGVADLITTCYGGRNRRVSEAFVTSGKTIEELEKEMLNGQKLQGPPTAEEVNYM 310
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L E++FPLFTAI+ IC ++ P IDC+RNHPEHM
Sbjct: 311 LKNKGLEDKFPLFTAIHKICINQLKPKDLIDCIRNHPEHM 350
>sp|P21695|GPDA_HUMAN Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 349
Score = 258 bits (658), Expect = 2e-68
Identities = 125/220 (56%), Positives = 161/220 (73%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS++I ++L IP + LMGAN+A EV+ E +CE TIG DPA+G LK L QT FR+ V
Sbjct: 131 LISEVIGERLGIPMSVLMGANIASEVADEKFCETTIGCKDPAQGQLLKELMQTPNFRITV 190
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
+++ E+CGALKN+VA+GAGF +GLG+GDNTKAA+IRLG MEMI FA F ++
Sbjct: 191 VQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFAKLFCSG-PVSS 249
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
TFLESCGVADLITTCYGGRN+++ +A TGKS+ QLE ELLNGQ QGP T++E+ +
Sbjct: 250 ATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKELLNGQKLQGPETARELYSI 309
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L ++FPLF A+Y +C P +FI CL+NHPEHM
Sbjct: 310 LQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQNHPEHM 349
>sp|O57656|GPDA_FUGRU Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 351
Score = 256 bits (655), Expect = 3e-68
Identities = 126/220 (57%), Positives = 162/220 (73%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LISD+I++KL I + LMGAN+A+EV+ E +CE TIG + + G LK L QT FR+ V
Sbjct: 132 LISDVIQEKLGITMSVLMGANIANEVADEKFCETTIGCKNKSHGPLLKELMQTINFRVTV 191
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
+++ E+CGALKNIVA+GAGF +GLG+GDNTKAA+IRLG MEMI FA F ++
Sbjct: 192 VEEYDVVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIAFARIFCTAGPVSS 251
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
TFLESCGVADLITTCYGGRN+++ KA V+TGKS+ +LE E+L GQ QGP T+ EV ++
Sbjct: 252 ATFLESCGVADLITTCYGGRNRKVAKAFVKTGKSIEELEKEMLEGQKLQGPATAAEVYRI 311
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L ++FPLF A+Y IC P S+FI CL+NHPEHM
Sbjct: 312 LKHKNLIDKFPLFNAVYQICYQGHPVSEFISCLQNHPEHM 351
>sp|O35077|GPDA_RAT Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 349
Score = 256 bits (654), Expect = 4e-68
Identities = 124/220 (56%), Positives = 161/220 (73%)
Frame = +3
Query: 3 LISDIIRDKLNIPCTALMGANLADEVSKEFYCEATIGSSDPARGLELKTLFQTSYFRMVV 182
LIS++I + L IP + LMGAN+A EV++E +CE TIG DPA+G LK L QT FR+ V
Sbjct: 131 LISEVIGESLGIPMSVLMGANIASEVAEEKFCETTIGCKDPAQGQLLKDLMQTPNFRITV 190
Query: 183 IKDEVGCELCGALKNIVALGAGFSEGLGYGDNTKAAIIRLGFMEMIKFANTFFGNRGINQ 362
+++ E+CGALKNIVA+GAGF +GLG+GDNTKAA+IRLG MEMI A F ++
Sbjct: 191 VQEVDTVEICGALKNIVAVGAGFCDGLGFGDNTKAAVIRLGLMEMIASAKLFCSG-SVSS 249
Query: 363 TTFLESCGVADLITTCYGGRNKRIGKALVETGKSVPQLELELLNGQSAQGPLTSKEVNQV 542
TFLESCGVADLITTCYGGRN+++ +A TGKS+ QLE E+LNGQ QGP T++E++ +
Sbjct: 250 ATFLESCGVADLITTCYGGRNRKVAEAFARTGKSIEQLEKEMLNGQKLQGPQTARELHSI 309
Query: 543 LAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLRNHPEHM 662
L ++FPLFTA+Y +C P + I CL+NHPEHM
Sbjct: 310 LQHKGLVDKFPLFTAVYKVCYEGQPVGEIIRCLQNHPEHM 349
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,513,536
Number of Sequences: 369166
Number of extensions: 1732669
Number of successful extensions: 5462
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5148
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5329
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6728100400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)