Planarian EST Database

DrC_00765

Cluster detail
GO Category : Not Available
Consensus Sequence :
>DrC_00765 AATATAATTATTCCGATTAAATCAATAAAAAATTTGAAACATCAACAGATCTGGCAAATA CATCTCATTTTTATACAAGATTTATTCTGGAGAGACGGCAGAAGCTCTGCAAAGTCGAGC AATCAATCCTCCTAATTGAAGATGCGTTGCTAATCCGTCAACAATGCGCATGTGAACCTC TCCGATTTCCTAAAAATGTCGCATTTTCATAAAGATCGCAATTAGTAAATTAGACCTTTA TAAAATTAATTTTCATAAATTCTGGAATTCCATCGTGATTCTTTGTCACCCGAAATATAA TCCCAACAATATCCTCACAAGCATAACCACATTTTACTAAATGGTTCAACGATTTGAACG CCTCGTTGAATTTTGAGTGCACACAATTTTCCAACATTGTTTTAATGAAGAGAGGGTGTG GTTCATCGCACACCTTGAATACGTTCTCACTAGATACTGAACCAGATCCCTGTAACAATC TAAATAGATAACACTCTGATTTACAAGAACATTTTTATTAATTTGTTAAAAATTATTTAA TAAAAGACTTATATCTATTTT

BLASTX 2.2.13 [Nov-27-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= DrC_00765
         (561 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P53033|RFC2_CHICK  Activator 1 40 kDa subunit (Replicatio...    94   2e-19
sp|P35250|RFC2_HUMAN  Activator 1 40 kDa subunit (Replicatio...    91   2e-18
sp|Q9WUK4|RFC2_MOUSE  Activator 1 40 kDa subunit (Replicatio...    89   7e-18
sp|P53034|RFC2_DROME  Activator 1 40 kDa subunit (Replicatio...    77   2e-14
sp|O94449|RFC4_SCHPO  Probable activator 1 subunit 4 (Replic...    63   4e-10
sp|P40339|RFC4_YEAST  Activator 1 37 kDa subunit (Replicatio...    57   4e-08
sp|Q8TSX5|RFCS_METAC  Replication factor C small subunit (RF...    41   0.002
sp|Q8PVY4|RFCS_METMA  Replication factor C small subunit (RF...    40   0.004
sp|Q58817|RFCS_METJA  Replication factor C small subunit (RF...    33   0.48 
sp|Q58587|YB88_METJA  Hypothetical protein MJ1188                  31   2.4

>sp|P53033|RFC2_CHICK Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
           (A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
          Length = 359

 Score = 94.4 bits (233), Expect = 2e-19
 Identities = 44/94 (46%), Positives = 67/94 (71%), Gaps = 1/94 (1%)
 Frame = -3

Query: 469 GSGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLVKCGYACEDIVGIIFR 290
           G G ++SENVFKVCDEPHPL +K M+++C+++  +EA+K L HL + GY+ ED++G IFR
Sbjct: 247 GFGFINSENVFKVCDEPHPLLVKEMIQHCINANIDEAYKILAHLWRLGYSPEDVIGNIFR 306

Query: 289 VTKNHDGIPEFMKINFIK-V*FTNCDLYENATFL 191
           V K    +PE++K+ FIK + +T+  + E    L
Sbjct: 307 VCKTFQ-MPEYLKLEFIKEIGYTHMKIAEGVNSL 339


 Score = 40.8 bits (94), Expect = 0.002
 Identities = 17/39 (43%), Positives = 28/39 (71%)
 Frame = -1

Query: 213 FMKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCRASA 97
           ++K+    EIG  HM+I +G+ + LQ+ GL+ARLC+ +A
Sbjct: 316 YLKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQKTA 354

>sp|P35250|RFC2_HUMAN Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
           (A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
          Length = 354

 Score = 90.9 bits (224), Expect = 2e-18
 Identities = 45/94 (47%), Positives = 65/94 (69%), Gaps = 1/94 (1%)
 Frame = -3

Query: 469 GSGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLVKCGYACEDIVGIIFR 290
           G G ++SENVFKVCDEPHPL +K M+++CV++  +EA+K L HL   GY+ EDI+G IFR
Sbjct: 242 GFGFINSENVFKVCDEPHPLLVKEMIQHCVNANIDEAYKILAHLWHLGYSPEDIIGNIFR 301

Query: 289 VTKNHDGIPEFMKINFIK-V*FTNCDLYENATFL 191
           V K    + E++K+ FIK + +T+  + E    L
Sbjct: 302 VCKTFQ-MAEYLKLEFIKEIGYTHMKIAEGVNSL 334


 Score = 40.0 bits (92), Expect = 0.004
 Identities = 17/39 (43%), Positives = 28/39 (71%)
 Frame = -1

Query: 222 IAIFMKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCR 106
           +A ++K+    EIG  HM+I +G+ + LQ+ GL+ARLC+
Sbjct: 308 MAEYLKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQ 346

>sp|Q9WUK4|RFC2_MOUSE Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
           (A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
          Length = 349

 Score = 89.0 bits (219), Expect = 7e-18
 Identities = 41/78 (52%), Positives = 57/78 (73%)
 Frame = -3

Query: 469 GSGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLVKCGYACEDIVGIIFR 290
           G G ++SENVFKVCDEPHPL +K M+++CV +  +EA+K L HL   GY+ ED++G IFR
Sbjct: 237 GFGYINSENVFKVCDEPHPLLVKEMIQHCVDANIDEAYKILAHLWHLGYSPEDVIGNIFR 296

Query: 289 VTKNHDGIPEFMKINFIK 236
           V K    + E++K+ FIK
Sbjct: 297 VCKTFP-MAEYLKLEFIK 313


 Score = 39.7 bits (91), Expect = 0.005
 Identities = 16/39 (41%), Positives = 28/39 (71%)
 Frame = -1

Query: 222 IAIFMKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCR 106
           +A ++K+    EIG  HM++ +G+ + LQ+ GL+ARLC+
Sbjct: 303 MAEYLKLEFIKEIGYTHMKVAEGVNSLLQMAGLLARLCQ 341

>sp|P53034|RFC2_DROME Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
           (A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
          Length = 331

 Score = 77.4 bits (189), Expect = 2e-14
 Identities = 37/79 (46%), Positives = 55/79 (69%)
 Frame = -3

Query: 472 QGSGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLVKCGYACEDIVGIIF 293
           QG G +++ENVFKVCDEPHP  ++ M+ +C  +  ++A+K L  L K GY+ EDI+  IF
Sbjct: 221 QGFGDITAENVFKVCDEPHPKLLEEMIHHCAANDIHKAYKILAKLWKLGYSPEDIIANIF 280

Query: 292 RVTKNHDGIPEFMKINFIK 236
           RV K  + I E +K++FI+
Sbjct: 281 RVCKRIN-IDEHLKLDFIR 298


 Score = 37.0 bits (84), Expect = 0.033
 Identities = 17/37 (45%), Positives = 26/37 (70%)
 Frame = -1

Query: 210 MKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCRAS 100
           +K+    EIG  HM+I+DG+ + LQL  L+A+LC A+
Sbjct: 292 LKLDFIREIGITHMKIIDGINSLLQLTALLAKLCIAA 328

>sp|O94449|RFC4_SCHPO Probable activator 1 subunit 4 (Replication factor C subunit 4)
           (Replication factor C4)
          Length = 342

 Score = 63.2 bits (152), Expect = 4e-10
 Identities = 31/78 (39%), Positives = 44/78 (56%)
 Frame = -3

Query: 469 GSGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLVKCGYACEDIVGIIFR 290
           G G V+ ENVF+V D+P P+ I  ML  C     + A + L  +   G++  DIV  +FR
Sbjct: 227 GFGLVNGENVFRVADQPSPVAIHAMLTACQSGNIDVALEKLQGIWDLGFSAVDIVTNMFR 286

Query: 289 VTKNHDGIPEFMKINFIK 236
           V K  D IPEF ++  +K
Sbjct: 287 VVKTMDSIPEFSRLEMLK 304


 Score = 37.7 bits (86), Expect = 0.020
 Identities = 19/43 (44%), Positives = 28/43 (65%)
 Frame = -1

Query: 213 FMKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCRASAVSPE 85
           F ++    EIG+ HM I++G+ T LQL GL+ RL + S + PE
Sbjct: 297 FSRLEMLKEIGQTHMIILEGVQTLLQLSGLVCRLAK-SQMKPE 338

>sp|P40339|RFC4_YEAST Activator 1 37 kDa subunit (Replication factor C subunit 4)
           (Replication factor C4)
          Length = 323

 Score = 56.6 bits (135), Expect = 4e-08
 Identities = 33/79 (41%), Positives = 47/79 (59%), Gaps = 1/79 (1%)
 Frame = -3

Query: 469 GSGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSL-NHLVKCGYACEDIVGIIF 293
           G G V+++NVFK+ D PHPL +K ML   + S   ++ + L   L K GY+  DIV   F
Sbjct: 215 GHGLVNADNVFKIVDSPHPLIVKKML---LASNLEDSIQILRTDLWKKGYSSIDIVTTSF 271

Query: 292 RVTKNHDGIPEFMKINFIK 236
           RVTKN   + E +++  IK
Sbjct: 272 RVTKNLAQVKESVRLEMIK 290


 Score = 33.9 bits (76), Expect = 0.28
 Identities = 13/26 (50%), Positives = 21/26 (80%)
 Frame = -1

Query: 189 EIGEVHMRIVDGLATHLQLGGLIARL 112
           EIG  HMRI++G+ T+LQL  ++A++
Sbjct: 291 EIGLTHMRILEGVGTYLQLASMLAKI 316

>sp|Q8TSX5|RFCS_METAC Replication factor C small subunit (RFC small subunit) (Clamp
           loader small subunit)
          Length = 338

 Score = 41.2 bits (95), Expect = 0.002
 Identities = 23/66 (34%), Positives = 36/66 (54%), Gaps = 1/66 (1%)
 Frame = -3

Query: 460 SVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLV-KCGYACEDIVGIIFRVT 284
           S+S E +++     +P  IK ++E  +   F  A K LN L+ + G + EDIVG I+RV 
Sbjct: 224 SISRETIYRTTATANPEEIKNLIETALRGNFRIARKELNRLLYEEGLSGEDIVGQIYRVV 283

Query: 283 KNHDGI 266
              D +
Sbjct: 284 SEMDNL 289

>sp|Q8PVY4|RFCS_METMA Replication factor C small subunit (RFC small subunit) (Clamp
           loader small subunit)
          Length = 338

 Score = 40.0 bits (92), Expect = 0.004
 Identities = 22/65 (33%), Positives = 35/65 (53%), Gaps = 1/65 (1%)
 Frame = -3

Query: 457 VSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHLV-KCGYACEDIVGIIFRVTK 281
           +S E +++     +P  IK ++E  +   F  A K LN L+ + G + EDIVG I+RV  
Sbjct: 225 ISRETIYRTTATANPEEIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVS 284

Query: 280 NHDGI 266
             D +
Sbjct: 285 EMDNL 289

>sp|Q58817|RFCS_METJA Replication factor C small subunit (RFC small subunit) (Clamp loader
            small subunit) [Contains: Mja RFC-1 intein; Mja RFC-2
            intein; Mja RFC-3 intein]
          Length = 1847

 Score = 33.1 bits (74), Expect = 0.48
 Identities = 23/66 (34%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
 Frame = -3

Query: 466  SGSVSSENVFKVCDEPHPLFIKTMLENCVHSKFNEAFKSLNHL-VKCGYACEDIVGIIFR 290
            S  +  E V+KV     P  +K M+E  +  KF EA   L  L V+ G + EDI+  +FR
Sbjct: 1740 SDVIDDEIVYKVSSRARPEEVKKMMELALDGKFMEARDLLYKLMVEWGMSGEDILNQMFR 1799

Query: 289  VTKNHD 272
               + D
Sbjct: 1800 EINSLD 1805

>sp|Q58587|YB88_METJA Hypothetical protein MJ1188
          Length = 270

 Score = 30.8 bits (68), Expect = 2.4
 Identities = 21/90 (23%), Positives = 39/90 (43%), Gaps = 6/90 (6%)
 Frame = +2

Query: 206 FIKIAISKLDLYKINFHKFWNSIVILCHPKYNPNNILTSITTFY*MVQRFERLVEF*VHT 385
           +++ A+ K  L+ I   KF+   +I  HP   P N   +   +    +  +R +E  ++ 
Sbjct: 79  YVREAVIKETLWSIELAKFYRCKLITIHPGKRPTNRSPTDEEYEAFFKYLDRTLEVAINK 138

Query: 386 IFQHCFNE--ERV----WFIAHLEYVLTRY 457
               C     ER+    W    +E++L RY
Sbjct: 139 NITICVENMPERINRIGWSPEEMEWILKRY 168

  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 60,651,210
Number of Sequences: 369166
Number of extensions: 1176051
Number of successful extensions: 2562
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2514
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2561
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4029688280
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)

Cluster detail

DrC_00765

Planaria EST Database

DrC_00765

BLASTX 2.2.13 [Nov-27-2005]

Cluster detail