Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_P21
(843 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q99490|CENG1_HUMAN Centaurin-gamma 1 188 2e-47
sp|Q9UPQ3|CENG2_HUMAN Centaurin-gamma 2 (ARF-GAP with GTP-b... 186 9e-47
sp|Q96P47|CENG3_HUMAN Centaurin-gamma 3 185 1e-46
sp|Q8BXK8|CENG2_MOUSE Centaurin-gamma 2 (ARF-GAP with GTP-b... 185 2e-46
sp|Q9NGC3|CEG1A_DROME Centaurin-gamma 1A 180 5e-45
sp|Q96P50|CENB5_HUMAN Centaurin-beta 5 (Cnt-b5) 140 4e-33
sp|Q15027|CENB1_HUMAN Centaurin-beta 1 (Cnt-b1) 132 9e-31
sp|Q15057|CENB2_HUMAN Centaurin-beta 2 (Cnt-b2) 122 9e-28
sp|Q9QWY8|DDEF1_MOUSE 130-kDa phosphatidylinositol 4,5-biph... 103 8e-22
sp|Q9ULH1|DDEF1_HUMAN 130-kDa phosphatidylinositol 4,5-biph... 102 1e-21
>sp|Q99490|CENG1_HUMAN Centaurin-gamma 1
Length = 836
Score = 188 bits (477), Expect = 2e-47
Identities = 97/224 (43%), Positives = 133/224 (59%), Gaps = 1/224 (0%)
Frame = +3
Query: 123 SKSKNNTSQ-DQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKK 299
SK++ + Q ++E++EF+++ + + WHF+A S EERD WV IES IL LQ +K
Sbjct: 506 SKTEGSAGQAEEENFEFLIVSSTGQTWHFEAASFEERDAWVQAIESQILASLQCCESSKV 565
Query: 300 SSDFQISSQNGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCW 479
+ SQ+ +Q IRN GN +C DCG+P+P W
Sbjct: 566 K--LRTDSQSEAVAIQ-----------------------AIRNAKGNSICVDCGAPNPTW 600
Query: 480 ASLNLGISVCIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVH 659
ASLNLG +CIECSG+HR LGTHLSRVRS LD+W +E V+ AIGN AN VWE+
Sbjct: 601 ASLNLGALICIECSGIHRNLGTHLSRVRSLDLDDWpreLTLVLTAIGNDTANRVWESDTR 660
Query: 660 ASSLKKPEADSPRADKEAWVRAKYEKKMFLPPLPYLDAPIQQQL 791
+ KP DS R ++E+W+RAKYE+ +FL PL + P+ +QL
Sbjct: 661 GRA--KPSRDSSREERESWIRAKYEQLLFLAPLSTSEEPLGRQL 702
>sp|Q9UPQ3|CENG2_HUMAN Centaurin-gamma 2 (ARF-GAP with GTP-binding protein-like, ankyrin
repeat and pleckstrin homology domains 1) (AGAP1)
Length = 857
Score = 186 bits (471), Expect = 9e-47
Identities = 100/215 (46%), Positives = 128/215 (59%)
Frame = +3
Query: 147 QDQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKKSSDFQISSQ 326
+ +E++EFI++ + WHF+A + EERD WV IES IL LQ +K S +++SQ
Sbjct: 549 EQEENFEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKS--RLTSQ 606
Query: 327 NGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCWASLNLGISV 506
+ LQ IRN+ GN C DC + +P WASLNLG +
Sbjct: 607 SEAMALQS-----------------------IRNMRGNSHCVDCETQNPNWASLNLGALM 643
Query: 507 CIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVHASSLKKPEA 686
CIECSG+HR LGTHLSRVRS LD+W E I VM +IGN LANSVWE S + KP
Sbjct: 644 CIECSGIHRNLGTHLSRVRSLDLDDWPIELIKVMSSIGNELANSVWEES--SQGRTKPSV 701
Query: 687 DSPRADKEAWVRAKYEKKMFLPPLPYLDAPIQQQL 791
DS R +KE W+RAKYE+K+FL PLP + + Q L
Sbjct: 702 DSTREEKERWIRAKYEQKLFLAPLPCTELSLGQHL 736
>sp|Q96P47|CENG3_HUMAN Centaurin-gamma 3
Length = 876
Score = 185 bits (470), Expect = 1e-46
Identities = 95/217 (43%), Positives = 130/217 (59%)
Frame = +3
Query: 141 TSQDQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKKSSDFQIS 320
T + +ES+EF+++ + WHF+A + EER+ WV +++ IL LQ K + ++
Sbjct: 565 TEEAEESFEFVVVSLTGQTWHFEASTAEERELWVQSVQAQILASLQGCRSAKDKT--RLG 622
Query: 321 SQNGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCWASLNLGI 500
+QN +Q +R + GN C DC +P+P WASLNLG
Sbjct: 623 NQNAALAVQ-----------------------AVRTVRGNSFCIDCDAPNPDWASLNLGA 659
Query: 501 SVCIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVHASSLKKP 680
+CIECSG+HR LG HLSRVRS LD+W E +AVM A+GN LANSVWE ++ S KP
Sbjct: 660 LMCIECSGIHRHLGAHLSRVRSLDLDDWPPELLAVMTAMGNALANSVWEGALGGYS--KP 717
Query: 681 EADSPRADKEAWVRAKYEKKMFLPPLPYLDAPIQQQL 791
D+ R +KE W+RAKYE+K+FL PLP D P+ QQL
Sbjct: 718 GPDACREEKERWIRAKYEQKLFLAPLPSSDVPLGQQL 754
>sp|Q8BXK8|CENG2_MOUSE Centaurin-gamma 2 (ARF-GAP with GTP-binding protein-like, ankyrin
repeat and pleckstrin homology domains 1) (AGAP1)
Length = 857
Score = 185 bits (469), Expect = 2e-46
Identities = 100/215 (46%), Positives = 127/215 (59%)
Frame = +3
Query: 147 QDQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKKSSDFQISSQ 326
+ +E+ EFI++ + WHF+A + EERD WV IES IL LQ +K S +++SQ
Sbjct: 549 EQEENLEFIIVSLTGQTWHFEATTYEERDAWVQAIESQILASLQSCESSKNKS--RLTSQ 606
Query: 327 NGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCWASLNLGISV 506
+ LQ IRN+ GN C DC + +P WASLNLG +
Sbjct: 607 SEAMALQS-----------------------IRNMRGNSHCVDCDTQNPNWASLNLGALM 643
Query: 507 CIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVHASSLKKPEA 686
CIECSG+HR LGTHLSRVRS LD+W E I VM +IGN LANSVWE + KP
Sbjct: 644 CIECSGIHRNLGTHLSRVRSLDLDDWPMELIKVMSSIGNELANSVWEEG--SQGRTKPSL 701
Query: 687 DSPRADKEAWVRAKYEKKMFLPPLPYLDAPIQQQL 791
DS R +KE W+RAKYE+K+FL PLP + + QQL
Sbjct: 702 DSTREEKERWIRAKYEQKLFLAPLPCTEFSLGQQL 736
>sp|Q9NGC3|CEG1A_DROME Centaurin-gamma 1A
Length = 995
Score = 180 bits (456), Expect = 5e-45
Identities = 92/212 (43%), Positives = 126/212 (59%)
Frame = +3
Query: 123 SKSKNNTSQDQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKKS 302
S K N + D + YEF ++ D++QWHF+A + EERD+WV +E I LQ +K
Sbjct: 635 SSVKANEADDNDGYEFYIVSLDSKQWHFEAANSEERDEWVAAVEQEIFKSLQSIESSKTK 694
Query: 303 SDFQISSQNGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCWA 482
Q +S + + A+ + + + GN C DCG+P+P WA
Sbjct: 695 ---QATSTD-----------------------LAAMLAIRQRVPGNGFCVDCGAPNPEWA 728
Query: 483 SLNLGISVCIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVHA 662
SLNLG+ +CIECSGVHR LG+H+S+VRS LD+W H++VM AIGN+LANSVWE++
Sbjct: 729 SLNLGVLMCIECSGVHRNLGSHISKVRSLGLDDWPSPHLSVMLAIGNSLANSVWESNTR- 787
Query: 663 SSLKKPEADSPRADKEAWVRAKYEKKMFLPPL 758
KP + + R DKE WVR+KYE K FL PL
Sbjct: 788 -QRVKPTSQASREDKERWVRSKYEAKEFLTPL 818
>sp|Q96P50|CENB5_HUMAN Centaurin-beta 5 (Cnt-b5)
Length = 759
Score = 140 bits (353), Expect = 4e-33
Identities = 63/135 (46%), Positives = 88/135 (65%), Gaps = 3/135 (2%)
Frame = +3
Query: 405 AVANLIRNIAGNEVCSDCGSPDPCWASLNLGISVCIECSGVHRQLGTHLSRVRSFQLDEW 584
+V ++++AGN C DCG PDP WAS+NLG+ +CIECSG+HR LG H S+VRS LD W
Sbjct: 362 SVLQRVQSVAGNSQCGDCGQPDPRWASINLGVLLCIECSGIHRSLGVHCSKVRSLTLDSW 421
Query: 585 SKEHIAVMQAIGNTLANSVWEASVHASSLKKPEADSPRADKEAWVRAKYEKKMFL---PP 755
E + +M +GN+ N ++EA + +KP A S R DKEAW++ KY +K FL P
Sbjct: 422 EPELLKLMCELGNSAVNQIYEAQCEGAGSRKPTASSSRQDKEAWIKDKYVEKKFLRKAPM 481
Query: 756 LPYLDAPIQQQLN*C 800
P L+AP + ++ C
Sbjct: 482 APALEAPRRWRVQKC 496
>sp|Q15027|CENB1_HUMAN Centaurin-beta 1 (Cnt-b1)
Length = 740
Score = 132 bits (333), Expect = 9e-31
Identities = 58/121 (47%), Positives = 80/121 (66%)
Frame = +3
Query: 399 VGAVANLIRNIAGNEVCSDCGSPDPCWASLNLGISVCIECSGVHRQLGTHLSRVRSFQLD 578
VG V ++++ GN C DC P P WAS+NLG+++CI+CSG+HR LG H S+VRS LD
Sbjct: 404 VGHVVAQVQSVDGNAQCCDCREPAPEWASINLGVTLCIQCSGIHRSLGVHFSKVRSLTLD 463
Query: 579 EWSKEHIAVMQAIGNTLANSVWEASVHASSLKKPEADSPRADKEAWVRAKYEKKMFLPPL 758
W E + +M +GN + N ++EA V A ++KKP R +KEAW+ AKY +K FL L
Sbjct: 464 SWEPELVKLMCELGNVIINQIYEARVEAMAVKKPGPSCSRQEKEAWIHAKYVEKKFLTKL 523
Query: 759 P 761
P
Sbjct: 524 P 524
>sp|Q15057|CENB2_HUMAN Centaurin-beta 2 (Cnt-b2)
Length = 778
Score = 122 bits (307), Expect = 9e-28
Identities = 58/123 (47%), Positives = 81/123 (65%)
Frame = +3
Query: 420 IRNIAGNEVCSDCGSPDPCWASLNLGISVCIECSGVHRQLGTHLSRVRSFQLDEWSKEHI 599
++ I GN C DCG DP WAS+NLGI++CIECSG+HR LG H S+VRS LD W E +
Sbjct: 405 VQCIPGNASCCDCGLADPRWASINLGITLCIECSGIHRSLGVHFSKVRSLTLDTWEPELL 464
Query: 600 AVMQAIGNTLANSVWEASVHASSLKKPEADSPRADKEAWVRAKYEKKMFLPPLPYLDAPI 779
+M +GN + N V+EA+V +KKP+ R +KEA++RAKY ++ F+ +P
Sbjct: 465 KLMCELGNDVINRVYEANVEKMGIKKPQ-PGQRQEKEAYIRAKYVERKFVDKYSISLSPP 523
Query: 780 QQQ 788
+QQ
Sbjct: 524 EQQ 526
>sp|Q9QWY8|DDEF1_MOUSE 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
GTPase-activating protein (PIP2-dependent ARF1 GAP)
(ADP-ribosylation factor-directed GTPase-activating
protein 1) (ARF GTPase-activating protein 1)
(Development and differentiation-enhancing factor 1)
(Differentiation-enhancing factor 1) (DEF-1)
Length = 1147
Score = 103 bits (256), Expect = 8e-22
Identities = 59/201 (29%), Positives = 102/201 (50%)
Frame = +3
Query: 144 SQDQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKKSSDFQISS 323
++D++S++ I + NR +HF A E+ D++ I L + + + F+
Sbjct: 394 AEDKKSFDLI---SHNRTYHFQA---EDEQDYIAWISVLTNSKEEAL-----TMAFRGEQ 442
Query: 324 QNGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCWASLNLGIS 503
G L+D A+ ++ + GN++C DCGS +P W S NLGI
Sbjct: 443 STGENSLED---------------LTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGIL 487
Query: 504 VCIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVHASSLKKPE 683
CIECSG+HR++G H+SR++S +LD+ + + + +GN N + EA++ + S KP
Sbjct: 488 TCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPS-PKPT 546
Query: 684 ADSPRADKEAWVRAKYEKKMF 746
S ++ ++ AKY F
Sbjct: 547 PSSDMTVRKEYITAKYVDHRF 567
>sp|Q9ULH1|DDEF1_HUMAN 130-kDa phosphatidylinositol 4,5-biphosphate-dependent ARF1
GTPase-activating protein (PIP2-dependent ARF1 GAP)
(ADP-ribosylation factor-directed GTPase-activating
protein 1) (ARF GTPase-activating protein 1)
(Development and differentiation-enhancing factor 1)
Length = 956
Score = 102 bits (255), Expect = 1e-21
Identities = 60/201 (29%), Positives = 102/201 (50%)
Frame = +3
Query: 144 SQDQESYEFILIGNDNRQWHFDAGSLEERDDWVVKIESLILLRLQIAPPNKKSSDFQISS 323
++D++S++ I + NR +HF A E+ D+V I L + + + F+
Sbjct: 206 AEDKKSFDLI---SHNRTYHFQA---EDEQDYVAWISVLTNSKEEAL-----TMAFRGEQ 254
Query: 324 QNGFTPLQDSPXXXXXXXXXXXXXXVGAVANLIRNIAGNEVCSDCGSPDPCWASLNLGIS 503
G L+D A+ ++ + GN++C DCGS +P W S NLGI
Sbjct: 255 SAGENSLED---------------LTKAIIEDVQRLPGNDICCDCGSSEPTWLSTNLGIL 299
Query: 504 VCIECSGVHRQLGTHLSRVRSFQLDEWSKEHIAVMQAIGNTLANSVWEASVHASSLKKPE 683
CIECSG+HR++G H+SR++S +LD+ + + + +GN N + EA++ + S KP
Sbjct: 300 TCIECSGIHREMGVHISRIQSLELDKLGTSELLLAKNVGNNSFNDIMEANLPSPS-PKPT 358
Query: 684 ADSPRADKEAWVRAKYEKKMF 746
S ++ ++ AKY F
Sbjct: 359 PSSDMTVRKEYITAKYVDHRF 379
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 87,116,673
Number of Sequences: 369166
Number of extensions: 1619447
Number of successful extensions: 4010
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3875
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3980
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8245425915
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)