Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_O08
(733 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q88P86|HIS8_PSEPK Histidinol-phosphate aminotransferase ... 33 1.0
sp|Q97ES6|HIS8_CLOAB Histidinol-phosphate aminotransferase ... 32 1.3
sp|Q11069|YT41_CAEEL Hypothetical protein B0416.1 in chromo... 32 1.7
sp|Q08352|DHA_BACSU Alanine dehydrogenase (Stage V sporulat... 31 3.0
sp|P22535|COA3_BPPRD Major capsid protein (Protein P3) 31 3.0
sp|Q8SL88|RPOA_EUGST DNA-directed RNA polymerase alpha chai... 31 3.0
sp|Q971A9|ILVC_SULTO Ketol-acid reductoisomerase (Acetohydr... 31 3.9
sp|Q73HA0|RS8_WOLPM 30S ribosomal protein S8 30 5.1
sp|Q87WV6|HIS8_PSESM Histidinol-phosphate aminotransferase ... 30 5.1
sp|P48582|BRO1_YEAST Vacuolar sorting protein BRO1 (BRO dom... 30 6.6
>sp|Q88P86|HIS8_PSEPK Histidinol-phosphate aminotransferase (Imidazole acetol-phosphate
transaminase)
Length = 348
Score = 32.7 bits (73), Expect = 1.0
Identities = 15/45 (33%), Positives = 23/45 (51%)
Frame = +2
Query: 467 KYDFAIVADTLQETRRILSTTKAYNSGDPRIYEPLKRVLNSLNQY 601
+YD +V TL ++R + G P + E L+R+ NS N Y
Sbjct: 198 RYDNLLVTQTLSKSRSLAGLRVGLAVGHPDLIEALERIKNSFNSY 242
>sp|Q97ES6|HIS8_CLOAB Histidinol-phosphate aminotransferase (Imidazole acetol-phosphate
transaminase)
Length = 352
Score = 32.3 bits (72), Expect = 1.3
Identities = 14/45 (31%), Positives = 23/45 (51%)
Frame = +2
Query: 470 YDFAIVADTLQETRRILSTTKAYNSGDPRIYEPLKRVLNSLNQYS 604
Y ++ TL ++R + + G P + E L RV NS+N Y+
Sbjct: 199 YKNLLIVQTLSKSRSLAGLRIGFAMGSPELIEGLSRVKNSINSYT 243
>sp|Q11069|YT41_CAEEL Hypothetical protein B0416.1 in chromosome X
Length = 1203
Score = 32.0 bits (71), Expect = 1.7
Identities = 18/50 (36%), Positives = 29/50 (58%)
Frame = +2
Query: 461 NVKYDFAIVADTLQETRRILSTTKAYNSGDPRIYEPLKRVLNSLNQYSNA 610
N++Y A V ++ Q+ R ST + GDP ++EPL LN + Y++A
Sbjct: 1053 NIRYGVATVENSSQDPTRPPSTDDSL--GDPALHEPLWANLNPHSSYTSA 1100
>sp|Q08352|DHA_BACSU Alanine dehydrogenase (Stage V sporulation protein N)
Length = 378
Score = 31.2 bits (69), Expect = 3.0
Identities = 14/36 (38%), Positives = 23/36 (63%)
Frame = -2
Query: 222 VLISVPKSIKDDFNKHEMKFGGAPYLFANSKRVMLE 115
++I VPK IK++ N+ + GG L +N RV++E
Sbjct: 1 MIIGVPKEIKNNENRVALTPGGVSQLISNGHRVLVE 36
>sp|P22535|COA3_BPPRD Major capsid protein (Protein P3)
Length = 395
Score = 31.2 bits (69), Expect = 3.0
Identities = 21/68 (30%), Positives = 35/68 (51%), Gaps = 2/68 (2%)
Frame = +2
Query: 50 FKIGKVIKAIKNYDPVNDKYKYSNMTLLEFAN--RYGAPPNFISCLLKSSLIDLGTEINT 223
F +++ + YDP N ++ ++ L F N + GAP F+S ++ S I G +N
Sbjct: 90 FGPANLVQRVIYYDPDNQRHTETSGWHLHFVNTAKQGAP--FLSSMVTDSPIKYGDVMNV 147
Query: 224 INAADAIA 247
I+A IA
Sbjct: 148 IDAPATIA 155
>sp|Q8SL88|RPOA_EUGST DNA-directed RNA polymerase alpha chain (PEP) (Plastid-encoded RNA
polymerase alpha subunit) (RNA polymerase alpha subunit)
Length = 213
Score = 31.2 bits (69), Expect = 3.0
Identities = 46/183 (25%), Positives = 73/183 (39%), Gaps = 12/183 (6%)
Frame = +2
Query: 68 IKAIKNYDPVNDKYKYSNMTLLEFAN-RYGAPPNFISCLLKSSLIDLGTEINTINAADAI 244
I IKNY Y N L +F+N A N + L L L ++I + +
Sbjct: 6 ISLIKNYFIEGSSYGLFNFDLQDFSNCDRVAWGNLLRRTLLKDLEGLRFGNSSIFISRSF 65
Query: 245 ARMQFYYVKTKNGLRSYHMVNQYSSMNQSKLLVDQIFNQLK------SKNTTFKTNYSLE 406
+++ +Y H VN+YS + Q + Q+F+ + SK KT+YS+
Sbjct: 66 SKIPYY-----------HRVNEYSDIEQVNPSLLQVFSNFRNLHIFSSKPFYRKTSYSM- 113
Query: 407 AFRVDPLSATISGDINNPNVKYDFAIVADTLQ-----ETRRILSTTKAYNSGDPRIYEPL 571
+V + S DI PN Y +V +Q R L G + + P+
Sbjct: 114 -IKVLTSRSYFSKDILLPNNLY---VVNPDVQLFEFLSKRYKLRIISEICKGSGKSFSPI 169
Query: 572 KRV 580
K+V
Sbjct: 170 KKV 172
>sp|Q971A9|ILVC_SULTO Ketol-acid reductoisomerase (Acetohydroxy-acid isomeroreductase)
(Alpha-keto-beta-hydroxylacil reductoisomerase)
Length = 332
Score = 30.8 bits (68), Expect = 3.9
Identities = 25/101 (24%), Positives = 43/101 (42%), Gaps = 5/101 (4%)
Frame = +2
Query: 311 YSSMNQSKLLVDQIFNQ-----LKSKNTTFKTNYSLEAFRVDPLSATISGDINNPNVKYD 475
Y ++N+ K++VD I+ + LK+ + T K T + N +VK
Sbjct: 226 YETINEMKMIVDLIYEKGFTGMLKAVSETAKYG-----------GFTAGKYVINEDVKKR 274
Query: 476 FAIVADTLQETRRILSTTKAYNSGDPRIYEPLKRVLNSLNQ 598
V D ++ + + YN G P + +K V NSL +
Sbjct: 275 MKEVLDRIRSGKFAEEWIEEYNKGAPTLVNGMKEVENSLEE 315
>sp|Q73HA0|RS8_WOLPM 30S ribosomal protein S8
Length = 131
Score = 30.4 bits (67), Expect = 5.1
Identities = 11/23 (47%), Positives = 16/23 (69%)
Frame = -2
Query: 225 IVLISVPKSIKDDFNKHEMKFGG 157
I +IS PK + D+N H++K GG
Sbjct: 102 IFIISTPKGVMTDYNAHKLKVGG 124
>sp|Q87WV6|HIS8_PSESM Histidinol-phosphate aminotransferase (Imidazole acetol-phosphate
transaminase)
Length = 350
Score = 30.4 bits (67), Expect = 5.1
Identities = 15/45 (33%), Positives = 22/45 (48%)
Frame = +2
Query: 467 KYDFAIVADTLQETRRILSTTKAYNSGDPRIYEPLKRVLNSLNQY 601
+Y +V TL ++R + G P + E L+RV NS N Y
Sbjct: 198 RYPNLLVTQTLSKSRSLAGLRVGLAVGHPDLIEALERVKNSFNSY 242
>sp|P48582|BRO1_YEAST Vacuolar sorting protein BRO1 (BRO domain protein 1) (BCK1-like
resistance to osmotic shock protein 1) (Vacuolar protein
sorting protein 31) (Amino acid sensor-independent
protein 6) (Nitrogen permease inactivating protein 3)
Length = 844
Score = 30.0 bits (66), Expect = 6.6
Identities = 13/43 (30%), Positives = 22/43 (51%)
Frame = +1
Query: 568 FKTSIEFIKSIFKCYKYFEHKILGSQNL*MQTENRLLKSIIVH 696
+K SI + F C++Y L S ++ +Q+EN + I H
Sbjct: 140 YKNSIANLTKAFSCFEYLSENFLNSPSVDLQSENTRFLANICH 182
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,355,767
Number of Sequences: 369166
Number of extensions: 1570372
Number of successful extensions: 3888
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3776
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3887
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6534486000
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)