Planarian EST Database


Dr_sW_028_N17

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_028_N17
         (799 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O51216|SPOT_BORBU  Guanosine-3',5'-bis(diphosphate) 3'-py...    31   3.4  
sp|P35424|VN53_ROTHW  Nonstructural RNA-binding protein 53 (...    30   9.9  
sp|Q96PC3|AP1S3_HUMAN  Adapter-related protein complex 1 sig...    30   9.9  
>sp|O51216|SPOT_BORBU Guanosine-3',5'-bis(diphosphate) 3'-pyrophosphohydrolase
           ((ppGpp)ase) (Penta-phosphate
           guanosine-3'-pyrophosphohydrolase)
          Length = 667

 Score = 31.2 bits (69), Expect = 3.4
 Identities = 30/116 (25%), Positives = 47/116 (40%), Gaps = 4/116 (3%)
 Frame = +2

Query: 341 LVNYQRNSKRMRQIHQAFSIATKLYATVPIKEWDKISEKVE----PHIRSQSWAFDSMFF 508
           L N  R+ K    I + F   T     + IK  DK+         P  R    A D +  
Sbjct: 123 LHNKTRSIKEANTISKMFFAMTHDIRIIIIKLADKLHNMTTLSYLPKNRQDRIAKDCLST 182

Query: 509 LDLALLRGPLVKSKTYLKDPRLKSIYPKPYRDLLYASINQCNRDKNKKTLKNLIDI 676
                 R  +   KTYL+D   K +YPK Y++ +   +++   ++ KK  K  + I
Sbjct: 183 YVPIAERLGISSLKTYLEDLSFKHLYPKDYKE-IKNFLSETKIEREKKLYKGKLSI 237
>sp|P35424|VN53_ROTHW Nonstructural RNA-binding protein 53 (NS53) (NCVP2)
          Length = 486

 Score = 29.6 bits (65), Expect = 9.9
 Identities = 19/60 (31%), Positives = 32/60 (53%), Gaps = 3/60 (5%)
 Frame = +2

Query: 545 SKTYLKDPRLKSIYPKPY---RDLLYASINQCNRDKNKKTLKNLIDILNICSLKLNENFK 715
           S T   D R+K IY   +   R L+ +++N  +R   +K  + + D+LN+C    NE +K
Sbjct: 329 SYTVWNDFRVKKIYDNIFSFLRALVKSNVNIGHRSSQEKIYEYVEDVLNVCD---NEKWK 385
>sp|Q96PC3|AP1S3_HUMAN Adapter-related protein complex 1 sigma 1C subunit (Sigma-adaptin
           1C) (Adaptor protein complex AP-1 sigma-1C subunit)
           (Golgi adaptor HA1/AP1 adaptin sigma-1C subunit)
           (Clathrin assembly protein complex 1 sigma-1C small
           chain) (Sigma 1C subunit of AP-1 clathrin)
          Length = 154

 Score = 29.6 bits (65), Expect = 9.9
 Identities = 32/127 (25%), Positives = 56/127 (44%), Gaps = 12/127 (9%)
 Frame = +2

Query: 407 KLYATVPIKEWDKISEKVEPHIRSQSWAFDSMFFLDLALLRGPLVKSKTYLKDPRLKSIY 586
           K Y T+P KE  KI+ ++                + + L RG   ++ +++    LK +Y
Sbjct: 18  KWYITLPDKERKKITREI----------------VQIILSRGH--RTSSFVDWKELKLVY 59

Query: 587 PKPYRDLLYASINQC----NRDKNKKTLK---NLIDIL-----NICSLKLNENFKECFDE 730
            +      YAS+  C    N+D    TL+     +++L     N+C L +  NF++ +  
Sbjct: 60  KR------YASLYFCCAIENQDNELLTLEIVHRYVELLDKYFGNVCELDIIFNFEKAYFI 113

Query: 731 LPEMITG 751
           L E I G
Sbjct: 114 LDEFIIG 120
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,530,449
Number of Sequences: 369166
Number of extensions: 1617026
Number of successful extensions: 3545
Number of sequences better than 10.0: 3
Number of HSP's better than 10.0 without gapping: 3455
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3545
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7522142940
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)