Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_N16
(882 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Int... 61 4e-09
sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6 60 1e-08
sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5 51 5e-06
sp|P15919|RAG1_MOUSE V(D)J recombination-activating protein... 50 6e-06
sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD4... 50 8e-06
sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RIN... 48 4e-05
sp|P15918|RAG1_HUMAN V(D)J recombination-activating protein... 47 5e-05
sp|P39429|TRAF2_MOUSE TNF receptor-associated factor 2 47 7e-05
sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD4... 47 9e-05
sp|Q8TBB1|LNX1_HUMAN Ubiquitin ligase LNX (Numb-binding pro... 46 1e-04
>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
(RING finger protein 85)
Length = 522
Score = 61.2 bits (147), Expect = 4e-09
Identities = 34/109 (31%), Positives = 49/109 (44%)
Frame = +1
Query: 97 KFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEECRENVFS 276
+FD + C C L A Q PCG +FC+ CI K I A CP E EN
Sbjct: 59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLEN--- 115
Query: 277 KNTNISVDYRTNNIIYKLKVKCPNESCNYECDFKSMNSHFEICKNSVLN 423
+ D I L VKCPNE C ++ + + + H C+ ++++
Sbjct: 116 ---QLFPDNFAKREILSLMVKCPNEGCLHKMELRHLEDHQAHCEFALMD 161
>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
Length = 530
Score = 59.7 bits (143), Expect = 1e-08
Identities = 34/109 (31%), Positives = 48/109 (44%)
Frame = +1
Query: 97 KFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEECRENVFS 276
+FD + C C L A Q PCG +FC+ CI K I A CP E EN
Sbjct: 59 EFDPPLESKYECPICLMALREAVQTPCGHRFCKACIIKSIRDAGHKCPVDNEILLEN--- 115
Query: 277 KNTNISVDYRTNNIIYKLKVKCPNESCNYECDFKSMNSHFEICKNSVLN 423
+ D I L VKCPN+ C + + + + H C+ +++N
Sbjct: 116 ---QLFPDNFAKREILSLTVKCPNKGCLQKMELRHLEDHQVHCEFALVN 161
>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
Length = 558
Score = 50.8 bits (120), Expect = 5e-06
Identities = 36/129 (27%), Positives = 56/129 (43%), Gaps = 15/129 (11%)
Frame = +1
Query: 85 NSSFKFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIE-HATEICPGSTEECR 261
++ ++F + + C FC +VL N HQ CG +FC+ CI L E ++ ICP E +
Sbjct: 30 DTEYQFVEQLEERYKCAFCHSVLHNPHQTGCGHRFCQQCIRSLRELNSVPICPVDKEVIK 89
Query: 262 -ENVFSKNTNISVDYRTNNIIYKLKVKCPN-ESCN------------YECDFKSMNSHFE 399
+ VF N + L V C N CN C F+++ E
Sbjct: 90 PQEVFKDNC-------CKREVLNLHVYCKNAPGCNARIILGRFQDHLQHCSFQAVPCPNE 142
Query: 400 ICKNSVLNK 426
C+ ++L K
Sbjct: 143 SCREAMLRK 151
>sp|P15919|RAG1_MOUSE V(D)J recombination-activating protein 1 (RAG-1)
Length = 1040
Score = 50.4 bits (119), Expect = 6e-06
Identities = 29/95 (30%), Positives = 42/95 (44%)
Frame = +1
Query: 130 CVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEECRENVFSKNTNISVDYRT 309
C C+++L + + C FCR+CI + ++ CP CR F + V
Sbjct: 290 CQICEHILADPVETSCKHLFCRICILRCLKVMGSYCP----SCRYPCFPTDLESPVK-SF 344
Query: 310 NNIIYKLKVKCPNESCNYECDFKSMNSHFEICKNS 414
NI+ L VKCP + CN E + N H K S
Sbjct: 345 LNILNSLMVKCPAQDCNEEVSLEKYNHHVSSHKES 379
>sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (TRAFAMN)
Length = 567
Score = 50.1 bits (118), Expect = 8e-06
Identities = 39/142 (27%), Positives = 55/142 (38%), Gaps = 15/142 (10%)
Frame = +1
Query: 97 KFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEECRENVFS 276
KF + D C C+ VL N Q CG +FC C+ L+ ++ C C+E++
Sbjct: 41 KFVKTVEDKYKCEKCRLVLCNPKQTECGHRFCESCMAALLSSSSPKCTA----CQESII- 95
Query: 277 KNTNISVDYRTNNIIYKLKVKCPNES---------------CNYECDFKSMNSHFEICKN 411
+ D I L+V C NE EC F+ + CK
Sbjct: 96 -KDKVFKDNCCKREILALQVYCRNEGRGCAEQLTLGHLLVHLKNECQFEELPCLRADCKE 154
Query: 412 SVLNKLKNDSDIETSCKDTELT 477
VL K D +E +CK E T
Sbjct: 155 KVLRKDLRD-HVEKACKYREAT 175
>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
Length = 557
Score = 47.8 bits (112), Expect = 4e-05
Identities = 36/125 (28%), Positives = 51/125 (40%), Gaps = 14/125 (11%)
Frame = +1
Query: 94 FKFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIE-HATEICPGSTEECRENV 270
++F + + C FC +VL N HQ CG +FC+ CI L E + ICP E
Sbjct: 33 YQFVERLEERYKCAFCHSVLHNPHQTGCGHRFCQHCILSLRELNTVPICPVDKE------ 86
Query: 271 FSKNTNISVDYRTNNIIYKLKVKCPN-ESCN------------YECDFKSMNSHFEICKN 411
K+ + D + L V C N CN +C F+ + E C+
Sbjct: 87 VIKSQEVFKDNCCKREVLNLYVYCSNAPGCNAKVILGRYQDHLQQCLFQPVQCSNEKCRE 146
Query: 412 SVLNK 426
VL K
Sbjct: 147 PVLRK 151
>sp|P15918|RAG1_HUMAN V(D)J recombination-activating protein 1 (RAG-1) (RING finger
protein 74)
Length = 1043
Score = 47.4 bits (111), Expect = 5e-05
Identities = 27/95 (28%), Positives = 42/95 (44%)
Frame = +1
Query: 130 CVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEECRENVFSKNTNISVDYRT 309
C C+++L + + C FCR+CI + ++ CP CR F + V
Sbjct: 293 CQICEHILADPVETNCKHVFCRVCILRCLKVMGSYCP----SCRYPCFPTDLESPVK-SF 347
Query: 310 NNIIYKLKVKCPNESCNYECDFKSMNSHFEICKNS 414
+++ L VKCP + CN E + N H K S
Sbjct: 348 LSVLNSLMVKCPAKECNEEVSLEKYNHHISSHKES 382
>sp|P39429|TRAF2_MOUSE TNF receptor-associated factor 2
Length = 501
Score = 47.0 bits (110), Expect = 7e-05
Identities = 30/120 (25%), Positives = 51/120 (42%), Gaps = 6/120 (5%)
Frame = +1
Query: 64 GMIKLLRNSSFK--FDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEIC 237
G ++LL+ K T + LC CKN+L QA CG ++C C+ ++ + C
Sbjct: 10 GSLELLQPGFSKTLLGTRLEAKYLCSACKNILRRPFQAQCGHRYCSFCLTSILSSGPQNC 69
Query: 238 PGSTEE--CRENV-FSKNTNISVDYRTNNIIYKLKVKCPNESCNYECDFKSMNS-HFEIC 405
E E + ++++ D + L CPN+ C ++ K S H +C
Sbjct: 70 AACVYEGLYEEGISILESSSAFPDNAARREVESLPAVCPNDGCTWKGTLKEYESCHEGLC 129
>sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (CD40 binding protein) (CD40BP) (LMP1
associated protein) (LAP1) (CAP-1)
Length = 568
Score = 46.6 bits (109), Expect = 9e-05
Identities = 38/143 (26%), Positives = 54/143 (37%), Gaps = 16/143 (11%)
Frame = +1
Query: 97 KFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEE-CRENVF 273
KF + D C C VL + Q CG +FC C+ L+ ++ C E ++ VF
Sbjct: 42 KFVKTVEDKYKCEKCHLVLCSPKQTECGHRFCESCMAALLSSSSPKCTACQESIVKDKVF 101
Query: 274 SKNTNISVDYRTNNIIYKLKVKCPNES---------------CNYECDFKSMNSHFEICK 408
N I L++ C NES +C F+ + CK
Sbjct: 102 KDNC-------CKREILALQIYCRNESRGCAEQLTLGHLLVHLKNDCHFEELPCVRPDCK 154
Query: 409 NSVLNKLKNDSDIETSCKDTELT 477
VL K D +E +CK E T
Sbjct: 155 EKVLRKDLRD-HVEKACKYREAT 176
>sp|Q8TBB1|LNX1_HUMAN Ubiquitin ligase LNX (Numb-binding protein 1) (Ligand of
Numb-protein X 1)
Length = 728
Score = 46.2 bits (108), Expect = 1e-04
Identities = 29/115 (25%), Positives = 48/115 (41%), Gaps = 2/115 (1%)
Frame = +1
Query: 76 LLRNSSFKFDTLISDDLLCVFCKNVLINAHQAPCGCKFCRLCIDKLIEHATEICPGSTEE 255
L N + + + DDL+C C L++ PCG +C LC+ + + CP +
Sbjct: 23 LEENHFYSYPEEVDDDLICHICLQALLDPLDTPCGHTYCTLCLTNFLVE-KDFCPMDRKP 81
Query: 256 CRENVFSKNTNISVDYRTNNIIYKLKVKCP-NESCNYECDFKSMNSHFEI-CKNS 414
K++ + N ++ KL V CP E C + HF+ CK +
Sbjct: 82 LVLQHCKKSSIL-----VNKLLNKLLVTCPFREHCTQVLQRCDLEHHFQTSCKGA 131
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.133 0.381
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 80,236,245
Number of Sequences: 369166
Number of extensions: 1470162
Number of successful extensions: 4742
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4556
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4730
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8790245790
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)