Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_N01
(573 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P50136|ODBA_MOUSE 2-oxoisovalerate dehydrogenase alpha s... 275 8e-74
sp|P12694|ODBA_HUMAN 2-oxoisovalerate dehydrogenase alpha s... 274 1e-73
sp|Q8HXY4|ODBA_MACFA 2-oxoisovalerate dehydrogenase alpha s... 274 1e-73
sp|P11960|ODBA_RAT 2-oxoisovalerate dehydrogenase alpha sub... 273 3e-73
sp|P11178|ODBA_BOVIN 2-oxoisovalerate dehydrogenase alpha s... 272 5e-73
sp|P37940|ODBA_BACSU 2-oxoisovalerate dehydrogenase alpha s... 125 7e-29
sp|Q820A6|ODPA_STAAN Pyruvate dehydrogenase E1 component, a... 116 5e-26
sp|Q6GHZ2|ODPA_STAAR Pyruvate dehydrogenase E1 component, a... 114 1e-25
sp|P09060|ODBA_PSEPU 2-oxoisovalerate dehydrogenase alpha s... 111 1e-24
sp|Q8CPN3|ODPA_STAES Pyruvate dehydrogenase E1 component, a... 109 4e-24
>sp|P50136|ODBA_MOUSE 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 442
Score = 275 bits (702), Expect = 8e-74
Identities = 128/190 (67%), Positives = 153/190 (80%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
+++ ++ MTLLN DRI+Y+SQR GRISFYMTN+GEEG +GSA AL + DL+FGQYRE
Sbjct: 99 VLKFYRSMTLLNTMDRILYESQREGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREA 158
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
GV ++R Y +E +SQCYGN ND GKGRQMPVHY K+ + +ISSPL TQ+ A GAAY
Sbjct: 159 GVLMYRDYPLELFMSQCYGNVNDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAY 218
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A KR VICYFG+GAASEGDAHA NF+ATL+CP+I+FCRNNGYAISTPTSEQYRG
Sbjct: 219 AAKRANANRIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRG 278
Query: 544 DGIASRAPGY 573
DGIA+R PGY
Sbjct: 279 DGIAARGPGY 288
>sp|P12694|ODBA_HUMAN 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha) (BCKDE1A)
Length = 445
Score = 274 bits (700), Expect = 1e-73
Identities = 127/190 (66%), Positives = 155/190 (81%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
+++++K MTLLN DRI+Y+SQR+GRISFYMTN+GEEG +GSA AL+ DL+FGQYRE
Sbjct: 102 VLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREA 161
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
GV ++R Y +E ++QCYGN +D GKGRQMPVHY K+ + +ISSPL TQ+ A GAAY
Sbjct: 162 GVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAY 221
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A KR VICYFG+GAASEGDAHA NF+ATL+CP+I+FCRNNGYAISTPTSEQYRG
Sbjct: 222 AAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRG 281
Query: 544 DGIASRAPGY 573
DGIA+R PGY
Sbjct: 282 DGIAARGPGY 291
>sp|Q8HXY4|ODBA_MACFA 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 445
Score = 274 bits (700), Expect = 1e-73
Identities = 127/190 (66%), Positives = 155/190 (81%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
+++++K MTLLN DRI+Y+SQR+GRISFYMTN+GEEG +GSA AL+ DL+FGQYRE
Sbjct: 102 VLKLYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDNTDLVFGQYREA 161
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
GV ++R Y +E ++QCYGN +D GKGRQMPVHY K+ + +ISSPL TQ+ A GAAY
Sbjct: 162 GVLMYRDYPLELFMAQCYGNISDLGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAY 221
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A KR VICYFG+GAASEGDAHA NF+ATL+CP+I+FCRNNGYAISTPTSEQYRG
Sbjct: 222 AAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRG 281
Query: 544 DGIASRAPGY 573
DGIA+R PGY
Sbjct: 282 DGIAARGPGY 291
>sp|P11960|ODBA_RAT 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 441
Score = 273 bits (697), Expect = 3e-73
Identities = 126/190 (66%), Positives = 155/190 (81%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
++++++ MTLLN DRI+Y+SQR+GRISFYMTN+GEEG +GSA AL + DL+FGQYRE
Sbjct: 98 VLKLYRSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALERTDLVFGQYREA 157
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
GV ++R Y +E ++QCYGN +D GKGRQMPVHY K+ + +ISSPL TQ+ A GAAY
Sbjct: 158 GVLMYRDYPLELFMAQCYGNVSDPGKGRQMPVHYGCKERHFVTISSPLATQIPQAVGAAY 217
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A KR VICYFG+GAASEGDAHA NF+ATL+CP+I+FCRNNGYAISTPTSEQYRG
Sbjct: 218 AAKRANANQIVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRG 277
Query: 544 DGIASRAPGY 573
DGIA+R PGY
Sbjct: 278 DGIAARGPGY 287
>sp|P11178|ODBA_BOVIN 2-oxoisovalerate dehydrogenase alpha subunit, mitochondrial
precursor (Branched-chain alpha-keto acid dehydrogenase
E1 component alpha chain) (BCKDH E1-alpha)
Length = 455
Score = 272 bits (695), Expect = 5e-73
Identities = 126/190 (66%), Positives = 154/190 (81%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
+++ +K MTLLN DRI+Y+SQR+GRISFYMTN+GEEG +GSA AL+ DL+FGQYRE
Sbjct: 112 VLKFYKSMTLLNTMDRILYESQRQGRISFYMTNYGEEGTHVGSAAALDDTDLVFGQYREA 171
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
GV ++R Y +E ++QCYGN +D GKGRQMPVHY ++ + +ISSPL TQ+ A GAAY
Sbjct: 172 GVLMYRDYPLELFMAQCYGNVSDLGKGRQMPVHYGCRERHFVTISSPLATQIPQAVGAAY 231
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A KR VICYFG+GAASEGDAHA NF+ATL+CP+I+FCRNNGYAISTPTSEQYRG
Sbjct: 232 AAKRANANRVVICYFGEGAASEGDAHAGFNFAATLECPIIFFCRNNGYAISTPTSEQYRG 291
Query: 544 DGIASRAPGY 573
DGIA+R PGY
Sbjct: 292 DGIAARGPGY 301
>sp|P37940|ODBA_BACSU 2-oxoisovalerate dehydrogenase alpha subunit (Branched-chain
alpha-keto acid dehydrogenase E1 component alpha chain)
(BCKDH E1-alpha)
Length = 330
Score = 125 bits (314), Expect = 7e-29
Identities = 69/191 (36%), Positives = 107/191 (56%), Gaps = 2/191 (1%)
Frame = +1
Query: 7 IEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKD-DLIFGQYRET 183
++M++ M L D M+ R G+I F ++ G+E AQ+G+A AL+++ D + YR+
Sbjct: 17 VDMYRTMLLARKIDERMWLLNRSGKIPFVISCQGQEAAQVGAAFALDREMDYVLPYYRDM 76
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKG-RQMPVHYTNKDINLFSISSPLGTQLLHAAGAA 360
GV L G T + ++ + D G RQMP H+ K + + SSP+ TQ+ HA G A
Sbjct: 77 GVVLAFGMTAKDLMMSGFAKAADPNSGGRQMPGHFGQKKNRIVTGSSPVTTQVPHAVGIA 136
Query: 361 YAFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYR 540
A + + + FG+G++++GD H NF+A PVI+ C NN YAIS P +Q
Sbjct: 137 LAGRMEKKDIAAFVTFGEGSSNQGDFHEGANFAAVHKLPVIFMCENNKYAISVPYDKQVA 196
Query: 541 GDGIASRAPGY 573
+ I+ RA GY
Sbjct: 197 CENISDRAIGY 207
>sp|Q820A6|ODPA_STAAN Pyruvate dehydrogenase E1 component, alpha subunit
sp|P60090|ODPA_STAAW Pyruvate dehydrogenase E1 component, alpha subunit
sp|P60089|ODPA_STAAM Pyruvate dehydrogenase E1 component, alpha subunit
sp|Q6GAC1|ODPA_STAAS Pyruvate dehydrogenase E1 component, alpha subunit
Length = 370
Score = 116 bits (290), Expect = 5e-26
Identities = 63/187 (33%), Positives = 97/187 (51%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
L+E+ ++M I D+ R+GR+ FY G+E +Q+ S AL K+D I YR+
Sbjct: 48 LVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILPGYRDV 107
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
+W G L++ + + KG Q P + +N S +G Q + AAG A+
Sbjct: 108 PQIIWHGLP----LTEAFLFSRGHFKGNQFP-----EGVNALSPQIIIGAQYIQAAGVAF 158
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A K+ I Y GDG +S+GD + +NF+A P I+ +NN YAISTP S+Q
Sbjct: 159 ALKKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA 218
Query: 544 DGIASRA 564
+ +A +A
Sbjct: 219 ETLAQKA 225
>sp|Q6GHZ2|ODPA_STAAR Pyruvate dehydrogenase E1 component, alpha subunit
Length = 370
Score = 114 bits (286), Expect = 1e-25
Identities = 62/187 (33%), Positives = 96/187 (51%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
L+E+ ++M I D+ R+GR+ FY G+E +Q+ S AL K+D I YR+
Sbjct: 48 LVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALEKEDYILPGYRDV 107
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
+W G L++ + + KG Q P + +N S +G Q + AG A+
Sbjct: 108 PQIIWHGLP----LTEAFLFSRGHFKGNQFP-----EGVNALSPQIIIGAQYIQTAGVAF 158
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
A K+ I Y GDG +S+GD + +NF+A P I+ +NN YAISTP S+Q
Sbjct: 159 ALKKRGKNAVAITYTGDGGSSQGDFYEGINFAAAYKAPAIFVIQNNNYAISTPRSKQTAA 218
Query: 544 DGIASRA 564
+ +A +A
Sbjct: 219 ETLAQKA 225
>sp|P09060|ODBA_PSEPU 2-oxoisovalerate dehydrogenase alpha subunit (Branched-chain
alpha-keto acid dehydrogenase E1 component alpha chain)
(BCKDH E1-alpha)
Length = 410
Score = 111 bits (278), Expect = 1e-24
Identities = 62/167 (37%), Positives = 91/167 (54%)
Frame = +1
Query: 19 KKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRETGVGLW 198
+ M IFD M +QR+ ++SFYM + GEE G A+ALN+ D+ F YR+ + +
Sbjct: 82 RAMLKTRIFDSRMVVAQRQKKMSFYMQSLGEEAIGSGQALALNRTDMCFPTYRQQSILMA 141
Query: 199 RGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAYAFKRN 378
R ++ M+ Q N+ D KGRQ+P+ Y+ ++ F+IS L TQ + A G A A
Sbjct: 142 RDVSLVEMICQLLSNERDPLKGRQLPIMYSVREAGFFTISGNLATQFVQAVGWAMASAIK 201
Query: 379 QNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAIST 519
+ + GDGA +E D H A+ F+ PVI NN +AIST
Sbjct: 202 GDTKIASAWIGDGATAESDFHTALTFAHVYRAPVILNVVNNQWAIST 248
>sp|Q8CPN3|ODPA_STAES Pyruvate dehydrogenase E1 component, alpha subunit
Length = 370
Score = 109 bits (273), Expect = 4e-24
Identities = 59/187 (31%), Positives = 93/187 (49%)
Frame = +1
Query: 4 LIEMFKKMTLLNIFDRIMYDSQRRGRISFYMTNFGEEGAQIGSAVALNKDDLIFGQYRET 183
L+E+ ++M I D+ R+GR+ FY G+E +Q+ S AL +D I YR+
Sbjct: 48 LVELMERMVWTRILDQRSISLNRQGRLGFYAPTAGQEASQLASQYALESEDFILPGYRDV 107
Query: 184 GVGLWRGYTIEGMLSQCYGNKNDYGKGRQMPVHYTNKDINLFSISSPLGTQLLHAAGAAY 363
+W G L+ + + KG Q P + +N S +G Q + AG A+
Sbjct: 108 PQIIWHGLP----LTDAFLFSRGHFKGNQFP-----EGVNALSPQIIIGAQYIQTAGVAF 158
Query: 364 AFKRNQNGLCVICYFGDGAASEGDAHAAMNFSATLDCPVIYFCRNNGYAISTPTSEQYRG 543
K+ I Y GDG +S+GD + +NF++ P I+ +NN YAISTP S+Q
Sbjct: 159 GLKKRGKNAVAITYTGDGGSSQGDFYEGINFASAYKAPAIFVIQNNNYAISTPRSKQTAA 218
Query: 544 DGIASRA 564
+ +A +A
Sbjct: 219 ETLAQKA 225
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 61,507,686
Number of Sequences: 369166
Number of extensions: 1141540
Number of successful extensions: 2631
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2576
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2612
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4226258440
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)