Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_M13
(645 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P42669|PUR_MOUSE Transcriptional activator protein PUR-a... 193 4e-49
sp|Q00577|PUR_HUMAN Transcriptional activator protein PUR-a... 193 4e-49
sp|Q921G8|GCP2_MOUSE Gamma-tubulin complex component 2 (GCP-2) 34 0.37
sp|O94833|BPAEA_HUMAN Bullous pemphigoid antigen 1, isoform... 33 0.48
sp|Q5GTA4|DXR_WOLTR 1-deoxy-D-xylulose 5-phosphate reductoi... 33 0.82
sp|Q9BSJ2|GCP2_HUMAN Gamma-tubulin complex component 2 (GCP... 32 1.1
sp|P15771|NUCL_CHICK Nucleolin (Protein C23) 32 1.8
sp|P08514|ITA2B_HUMAN Integrin alpha-IIb precursor (Platele... 31 2.4
sp|O85709|RELA_STRAT GTP pyrophosphokinase (ATP:GTP 3'-pyro... 31 2.4
sp|Q09289|YQK7_CAEEL Hypothetical protein C56G2.7 in chromo... 30 5.3
>sp|P42669|PUR_MOUSE Transcriptional activator protein PUR-alpha (Purine-rich
single-stranded DNA-binding protein alpha)
Length = 321
Score = 193 bits (490), Expect = 4e-49
Identities = 105/203 (51%), Positives = 134/203 (66%), Gaps = 12/203 (5%)
Frame = +1
Query: 70 QSAEQELTSKTLMIQNKRFYLDVKQNLRGKFIKIAEVGIGGRKSRILMSLSTAAEFRDKL 249
Q QEL SK + IQNKRFYLDVKQN +G+F+KIAEVG GG KSR+ +S+S A EFRD L
Sbjct: 54 QHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYL 113
Query: 250 NDLSNCYNELAPHNAENLA-----ADGRLQSDTIVKDTRRYYLDLKENARGRFLRVTM-- 408
D Y +L P +LA L+S+ +V++ R+YY+DLKEN RGRFLR+
Sbjct: 114 GDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTV 173
Query: 409 -----VAPSNRSQIAIPAQGIIELRNALTELIKEYGGEENGSEDVELPEPKSFRIDNKMF 573
+ + IA+PAQG+IE R+AL +LI +YG EE E ELPE S +DNK F
Sbjct: 174 NRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEE---EPAELPEGTSLTVDNKRF 230
Query: 574 YFDVGCNRRGVFLRVSEVRTNYR 642
+FDVG N+ GVF+RVSEV+ YR
Sbjct: 231 FFDVGSNKYGVFMRVSEVKPTYR 253
Score = 60.5 bits (145), Expect = 4e-09
Identities = 38/134 (28%), Positives = 70/134 (52%), Gaps = 6/134 (4%)
Frame = +1
Query: 61 QNNQSAEQELTSKTLMIQNKRFYLDVKQNLRGKFIKIAEV-----GIGGRKSR-ILMSLS 222
Q + L S+ L+ +N+++Y+D+K+N RG+F++I + G+G + + I +
Sbjct: 133 QAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQ 192
Query: 223 TAAEFRDKLNDLSNCYNELAPHNAENLAADGRLQSDTIVKDTRRYYLDLKENARGRFLRV 402
EFRD L L + Y E A+ + ++ D +R++ D+ N G F+RV
Sbjct: 193 GLIEFRDALAKLIDDY------GVEEEPAE-LPEGTSLTVDNKRFFFDVGSNKYGVFMRV 245
Query: 403 TMVAPSNRSQIAIP 444
+ V P+ R+ I +P
Sbjct: 246 SEVKPTYRNSITVP 259
>sp|Q00577|PUR_HUMAN Transcriptional activator protein PUR-alpha (Purine-rich
single-stranded DNA-binding protein alpha)
Length = 322
Score = 193 bits (490), Expect = 4e-49
Identities = 105/203 (51%), Positives = 134/203 (66%), Gaps = 12/203 (5%)
Frame = +1
Query: 70 QSAEQELTSKTLMIQNKRFYLDVKQNLRGKFIKIAEVGIGGRKSRILMSLSTAAEFRDKL 249
Q QEL SK + IQNKRFYLDVKQN +G+F+KIAEVG GG KSR+ +S+S A EFRD L
Sbjct: 55 QHETQELASKRVDIQNKRFYLDVKQNAKGRFLKIAEVGAGGNKSRLTLSMSVAVEFRDYL 114
Query: 250 NDLSNCYNELAPHNAENLA-----ADGRLQSDTIVKDTRRYYLDLKENARGRFLRVTM-- 408
D Y +L P +LA L+S+ +V++ R+YY+DLKEN RGRFLR+
Sbjct: 115 GDFIEHYAQLGPSQPPDLAQAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTV 174
Query: 409 -----VAPSNRSQIAIPAQGIIELRNALTELIKEYGGEENGSEDVELPEPKSFRIDNKMF 573
+ + IA+PAQG+IE R+AL +LI +YG EE E ELPE S +DNK F
Sbjct: 175 NRGPGLGSTQGQTIALPAQGLIEFRDALAKLIDDYGVEE---EPAELPEGTSLTVDNKRF 231
Query: 574 YFDVGCNRRGVFLRVSEVRTNYR 642
+FDVG N+ GVF+RVSEV+ YR
Sbjct: 232 FFDVGSNKYGVFMRVSEVKPTYR 254
Score = 60.5 bits (145), Expect = 4e-09
Identities = 38/134 (28%), Positives = 70/134 (52%), Gaps = 6/134 (4%)
Frame = +1
Query: 61 QNNQSAEQELTSKTLMIQNKRFYLDVKQNLRGKFIKIAEV-----GIGGRKSR-ILMSLS 222
Q + L S+ L+ +N+++Y+D+K+N RG+F++I + G+G + + I +
Sbjct: 134 QAQDEPRRALKSEFLVRENRKYYMDLKENQRGRFLRIRQTVNRGPGLGSTQGQTIALPAQ 193
Query: 223 TAAEFRDKLNDLSNCYNELAPHNAENLAADGRLQSDTIVKDTRRYYLDLKENARGRFLRV 402
EFRD L L + Y E A+ + ++ D +R++ D+ N G F+RV
Sbjct: 194 GLIEFRDALAKLIDDY------GVEEEPAE-LPEGTSLTVDNKRFFFDVGSNKYGVFMRV 246
Query: 403 TMVAPSNRSQIAIP 444
+ V P+ R+ I +P
Sbjct: 247 SEVKPTYRNSITVP 260
>sp|Q921G8|GCP2_MOUSE Gamma-tubulin complex component 2 (GCP-2)
Length = 905
Score = 33.9 bits (76), Expect = 0.37
Identities = 38/132 (28%), Positives = 53/132 (40%), Gaps = 1/132 (0%)
Frame = +1
Query: 127 YLDVKQNLRGKFIKIAEVGIGGRKSRILMSLSTAAEFRDKLNDLSNCYNELAPHNAENLA 306
Y+D+ Q R ++ V K +I AEF D Y+EL N NL
Sbjct: 28 YIDLLQKNRTPYVTTT-VSAHSAKVKI-------AEFSRTPEDFLKKYDELKSKNTRNLD 79
Query: 307 ADGRLQSD-TIVKDTRRYYLDLKENARGRFLRVTMVAPSNRSQIAIPAQGIIELRNALTE 483
L S T K+T ++ L++NA+ R A SN + +IP L E
Sbjct: 80 PLVYLLSKLTEDKETLQF---LQQNAKERAELAASAATSNTTSFSIPVAASKMSTQELEE 136
Query: 484 LIKEYGGEENGS 519
L K+ G GS
Sbjct: 137 LRKQLGSVSTGS 148
>sp|O94833|BPAEA_HUMAN Bullous pemphigoid antigen 1, isoforms 6/9/10 (Trabeculin-beta)
(Bullous pemphigoid antigen) (BPA) (Hemidesmosomal plaque
protein) (Dystonia musculorum protein) (Dystonin)
Length = 5171
Score = 33.5 bits (75), Expect = 0.48
Identities = 30/144 (20%), Positives = 61/144 (42%)
Frame = +1
Query: 67 NQSAEQELTSKTLMIQNKRFYLDVKQNLRGKFIKIAEVGIGGRKSRILMSLSTAAEFRDK 246
N +A Q++ SK +M++ Q++ G+ I + +K STA+ + K
Sbjct: 1777 NSTALQDIISKNIMLE---------QDIAGRQSSINAMNEKVKKFMETTDPSTASSLQAK 1827
Query: 247 LNDLSNCYNELAPHNAENLAADGRLQSDTIVKDTRRYYLDLKENARGRFLRVTMVAPSNR 426
+ DLS ++E + + E LA L++ ++L EN +
Sbjct: 1828 MKDLSARFSEASHKHKETLAKMEELKTK----------VELFENLSEKLQTFLETKTQAL 1877
Query: 427 SQIAIPAQGIIELRNALTELIKEY 498
+++ +P + + EL + E E+
Sbjct: 1878 TEVDVPGKDVTELSQYMQESTSEF 1901
>sp|Q5GTA4|DXR_WOLTR 1-deoxy-D-xylulose 5-phosphate reductoisomerase (DXP
reductoisomerase) (1-deoxyxylulose-5-phosphate
reductoisomerase) (2-C-methyl-D-erythritol 4-phosphate
synthase)
Length = 389
Score = 32.7 bits (73), Expect = 0.82
Identities = 17/35 (48%), Positives = 24/35 (68%)
Frame = +1
Query: 88 LTSKTLMIQNKRFYLDVKQNLRGKFIKIAEVGIGG 192
L ++ + I NKRFY D+K++L G IK+ EVG G
Sbjct: 49 LNARYVAISNKRFYKDLKESLLGTGIKV-EVGTEG 82
>sp|Q9BSJ2|GCP2_HUMAN Gamma-tubulin complex component 2 (GCP-2) (Spindle pole body
protein Spc97 homolog) (hSpc97) (hGCP2) (h103p)
Length = 902
Score = 32.3 bits (72), Expect = 1.1
Identities = 38/132 (28%), Positives = 53/132 (40%), Gaps = 1/132 (0%)
Frame = +1
Query: 127 YLDVKQNLRGKFIKIAEVGIGGRKSRILMSLSTAAEFRDKLNDLSNCYNELAPHNAENLA 306
Y+D+ Q R ++ V K +I AEF D Y+EL N NL
Sbjct: 28 YIDLLQKNRTPYVTTT-VSAHSAKVKI-------AEFSRTPEDFLKKYDELKSKNTRNLD 79
Query: 307 ADGRLQSD-TIVKDTRRYYLDLKENARGRFLRVTMVAPSNRSQIAIPAQGIIELRNALTE 483
L S T K+T +Y L++NA+ R S+ + I +PA L E
Sbjct: 80 PLVYLLSKLTEDKETLQY---LQQNAKERAELAAAAVGSSTTSINVPAAASKISMQELEE 136
Query: 484 LIKEYGGEENGS 519
L K+ G GS
Sbjct: 137 LRKQLGSVATGS 148
>sp|P15771|NUCL_CHICK Nucleolin (Protein C23)
Length = 694
Score = 31.6 bits (70), Expect = 1.8
Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 5/79 (6%)
Frame = +1
Query: 43 TGKAKQQNNQSAEQELTSKTLMIQNKRFYLDVKQNLRGKF-----IKIAEVGIGGRKSRI 207
TG+ QQ +Q E SKTL++ N Y ++ L+ F IK+ + G K
Sbjct: 443 TGEKSQQESQKGGGERESKTLIVNNLS-YAASEETLQELFKKATSIKMPQNNQGRPKGYA 501
Query: 208 LMSLSTAAEFRDKLNDLSN 264
+ TA + ++ LN +N
Sbjct: 502 FVEFPTAEDAKEALNSCNN 520
>sp|P08514|ITA2B_HUMAN Integrin alpha-IIb precursor (Platelet membrane glycoprotein IIb)
(GPalpha IIb) (GPIIb) (CD41 antigen) [Contains: Integrin
alpha-IIb heavy chain; Integrin alpha-IIb light chain]
Length = 1039
Score = 31.2 bits (69), Expect = 2.4
Identities = 27/99 (27%), Positives = 45/99 (45%), Gaps = 4/99 (4%)
Frame = +1
Query: 184 IGGRKSRI----LMSLSTAAEFRDKLNDLSNCYNELAPHNAENLAADGRLQSDTIVKDTR 351
+GG+ S I + L A+FRDKL+ + N P +A L DT V++
Sbjct: 568 LGGKHSPICHTTMAFLRDEADFRDKLSPIVLSLNVSLPPTEAGMAPAVVLHGDTHVQEQT 627
Query: 352 RYYLDLKENARGRFLRVTMVAPSNRSQIAIPAQGIIELR 468
R LD E+ ++ + A S + + A ++EL+
Sbjct: 628 RIVLDCGED-DVCVPQLQLTASVTGSPLLVGADNVLELQ 665
>sp|O85709|RELA_STRAT GTP pyrophosphokinase (ATP:GTP 3'-pyrophosphotransferase) (ppGpp
synthetase I) ((P)ppGpp synthetase)
Length = 841
Score = 31.2 bits (69), Expect = 2.4
Identities = 25/96 (26%), Positives = 41/96 (42%), Gaps = 3/96 (3%)
Frame = +1
Query: 328 DTIVKDTRRYYLDLKENARGRFLRVT---MVAPSNRSQIAIPAQGIIELRNALTELIKEY 498
D I + R+ L ++ G L M P S A +G + +N + +L++
Sbjct: 603 DAIARAMRKQNLPIQRILTGDSLVTLAHEMRYPDISSSDAAIGEGHVGAQNVVQKLVQAL 662
Query: 499 GGEENGSEDVELPEPKSFRIDNKMFYFDVGCNRRGV 606
GGEE SE+++ P R + D G +GV
Sbjct: 663 GGEEAASEEIDEAVPSRSRSRKRRSNQDPGVVVKGV 698
>sp|Q09289|YQK7_CAEEL Hypothetical protein C56G2.7 in chromosome III
Length = 374
Score = 30.0 bits (66), Expect = 5.3
Identities = 34/131 (25%), Positives = 50/131 (38%), Gaps = 6/131 (4%)
Frame = +1
Query: 193 RKSRILMSLSTAAEFRDKLNDLSNCYNELAPHNAENLAADGRLQSDTIVKDTRRYYLDLK 372
+K + +SL+TA N ELAPH R S+T+ R D
Sbjct: 240 QKKEVAVSLATALSNETVAEVARNHAEELAPH-LPTSDDPARELSETVRTPQFRQAADTL 298
Query: 373 ENARGRFLRVTMVAPSNRSQIAIPAQGIIELRNALTELIKEYGGE-----ENGSED-VEL 534
+A +VA + + + ++R L K GGE +N +D
Sbjct: 299 GHALQTGQLGPVVAQFGMDEATVGSANQGDIRGFAANLTKAEGGEDAAKTQNSDDDATRE 358
Query: 535 PEPKSFRIDNK 567
PEPK R DN+
Sbjct: 359 PEPKRNRPDNE 369
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 57,461,091
Number of Sequences: 369166
Number of extensions: 938734
Number of successful extensions: 2798
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2713
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2786
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 5267491560
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)