Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_L18
(505 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P27694|RFA1_HUMAN Replication protein A 70 kDa DNA-bindi... 125 4e-29
sp|Q01588|RFA1_XENLA Replication protein A 70 kDa DNA-bindi... 115 5e-26
sp|Q24492|RFA1_DROME Replication protein A 70 kDa DNA-bindi... 105 5e-23
sp|P22336|RFA1_YEAST Replication factor-A protein 1 (RF-A) ... 91 2e-18
sp|Q92372|RFA1_SCHPO Replication factor-A protein 1 (Single... 89 5e-18
sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa s... 80 2e-15
sp|Q9JYV5|FRPC_NEIMB Iron-regulated protein frpC 32 0.86
sp|P55127|FRPC_NEIMC Iron-regulated protein frpC 31 1.5
sp|Q9K0K9|FRPA_NEIMB Iron-regulated protein frpA 31 1.9
sp|Q9C646|RX24L_ARATH Probable disease resistance protein R... 30 2.5
>sp|P27694|RFA1_HUMAN Replication protein A 70 kDa DNA-binding subunit (RP-A) (RF-A)
(Replication factor-A protein 1) (Single-stranded
DNA-binding protein)
Length = 616
Score = 125 bits (315), Expect = 4e-29
Identities = 63/140 (45%), Positives = 93/140 (66%), Gaps = 2/140 (1%)
Frame = +2
Query: 8 VYKACQTEKCNKKLSDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVG 187
+Y+AC T+ CNKK+ D NGLY+CE+C+TE +F YR+IL+++I DF WVT FQE
Sbjct: 477 MYQACPTQDCNKKVIDQQNGLYRCEKCDTEFPNFKYRMILSVNIADFQENQWVTCFQESA 536
Query: 188 QLVLGKTAEDIGNMFELEQKEE--LENMFDEALYKDFDFKLKVKNDTYNDETRMRVTVID 361
+ +LG+ A +G EL+ K E E +F A ++ F F+++VK +TYNDE+R++ TV+D
Sbjct: 537 EAILGQNAAYLG---ELKDKNEQAFEEVFQNANFRSFIFRVRVKVETYNDESRIKATVMD 593
Query: 362 AKEVNVLESIERLLGQINVS 421
K V+ E RL+ I S
Sbjct: 594 VKPVDYREYGRRLVMSIRRS 613
>sp|Q01588|RFA1_XENLA Replication protein A 70 kDa DNA-binding subunit (RP-A) (RF-A)
(Replication factor-A protein 1) (Single-stranded
DNA-binding protein)
Length = 609
Score = 115 bits (289), Expect = 5e-26
Identities = 53/135 (39%), Positives = 87/135 (64%)
Frame = +2
Query: 8 VYKACQTEKCNKKLSDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVG 187
+Y+AC ++ CNKK+ D NGL++CE+C E +F YRLIL+ +I DF W+T FQE
Sbjct: 468 LYQACPSQDCNKKVIDQQNGLFRCEKCNKEFPNFKYRLILSANIADFGENQWITCFQESA 527
Query: 188 QLVLGKTAEDIGNMFELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVTVIDAK 367
+ +LG+ A +G + E + ++ + +F A ++ + F+ +VK +TYNDE+R++ T +D K
Sbjct: 528 ESILGQNATYLGELKE-KNEQAYDEVFQNANFRSYTFRARVKLETYNDESRIKATAVDVK 586
Query: 368 EVNVLESIERLLGQI 412
V+ E RL+ I
Sbjct: 587 PVDHKEYSRRLIMNI 601
>sp|Q24492|RFA1_DROME Replication protein A 70 kDa DNA-binding subunit (RP-A) (RF-A)
(Replication factor-A protein 1) (Single-stranded
DNA-binding protein) (DmRPA1)
Length = 603
Score = 105 bits (263), Expect = 5e-23
Identities = 51/134 (38%), Positives = 79/134 (58%)
Frame = +2
Query: 11 YKACQTEKCNKKLSDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVGQ 190
Y+AC CNKK+ D GN ++CE+C +F YRL++NMSI D+T WV++F EVG+
Sbjct: 461 YRACPQSDCNKKVVDEGNDQFRCEKCNALFPNFKYRLLINMSIGDWTSNRWVSSFNEVGE 520
Query: 191 LVLGKTAEDIGNMFELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVTVIDAKE 370
+LG T++++G E + E +F + FKL+ KN+ Y D TR ++TV
Sbjct: 521 QLLGHTSQEVGEALE-NDPAKAEQIFSALNFTSHIFKLRCKNEVYGDMTRNKLTVQSVAP 579
Query: 371 VNVLESIERLLGQI 412
+N E + LL ++
Sbjct: 580 INHKEYNKHLLKEL 593
>sp|P22336|RFA1_YEAST Replication factor-A protein 1 (RF-A) (Single-stranded DNA-binding
protein) (DNA binding protein BUF2) (Replication protein
A 69 kDa DNA-binding subunit)
Length = 621
Score = 90.5 bits (223), Expect = 2e-18
Identities = 43/135 (31%), Positives = 76/135 (56%)
Frame = +2
Query: 11 YKACQTEKCNKKLSDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVGQ 190
Y AC E CNKK+ + +G ++CE+C+T + +R IL +SIID T W+T F + +
Sbjct: 483 YPACSNENCNKKVLEQPDGTWRCEKCDTNNARPNWRYILTISIIDETNQLWLTLFDDQAK 542
Query: 191 LVLGKTAEDIGNMFELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVTVIDAKE 370
+LG A + ++ E E E + ++DF+++ + DTYND++R+R TV +
Sbjct: 543 QLLGVDANTLMSLKE-EDPNEFTKITQSIQMNEYDFRIRAREDTYNDQSRIRYTVANLHS 601
Query: 371 VNVLESIERLLGQIN 415
+N + L +++
Sbjct: 602 LNYRAEADYLADELS 616
>sp|Q92372|RFA1_SCHPO Replication factor-A protein 1 (Single-stranded DNA-binding protein
p68 subunit)
Length = 609
Score = 89.4 bits (220), Expect = 5e-18
Identities = 45/134 (33%), Positives = 77/134 (57%)
Frame = +2
Query: 11 YKACQTEKCNKKLSDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVGQ 190
Y AC CNKK+ D G G ++CE+C E YR I+ +++ D TG W+ F +VG+
Sbjct: 474 YPACPAADCNKKVFDQG-GSWRCEKCNKEYDAPQYRYIITIAVGDHTGQLWLNVFDDVGK 532
Query: 191 LVLGKTAEDIGNMFELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVTVIDAKE 370
L++ KTA+++ ++ E ++ + N EA Y + F+ + K D + E R+R TV+ +
Sbjct: 533 LIMHKTADELNDLQENDENAFM-NCMAEACYMPYIFQCRAKQDNFKGEMRVRYTVMSINQ 591
Query: 371 VNVLESIERLLGQI 412
++ E +RL+ I
Sbjct: 592 MDWKEESKRLINFI 605
>sp|Q19537|RFA1_CAEEL Probable replication factor A 73 kDa subunit (RP-A) (RF-A)
(Replication factor-A protein 1)
Length = 655
Score = 80.5 bits (197), Expect = 2e-15
Identities = 42/131 (32%), Positives = 74/131 (56%)
Frame = +2
Query: 8 VYKACQTEKCNKKLSDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVG 187
+Y+ C +E C KKL NG Y+CE+C + F + ++ + D TG +VTAF +
Sbjct: 514 LYRGCASEGCQKKLVG-ENGDYRCEKCNKNMNKFKWLYMMQFELSDETGQVYVTAFGDSA 572
Query: 188 QLVLGKTAEDIGNMFELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVTVIDAK 367
++GK+A ++G + + E +E +F+ + ++L+ K D+YN+E R ++TV
Sbjct: 573 AKIVGKSAAELGELHD-ESPDEYNAIFERLQFVPKMWRLRCKMDSYNEEVRQKMTVYGVD 631
Query: 368 EVNVLESIERL 400
+VN + IE L
Sbjct: 632 DVNQDKYIENL 642
>sp|Q9JYV5|FRPC_NEIMB Iron-regulated protein frpC
Length = 1829
Score = 32.0 bits (71), Expect = 0.86
Identities = 25/101 (24%), Positives = 42/101 (41%)
Frame = +2
Query: 50 SDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVGQLVLGKTAEDIGNM 229
S++ YK S A L++N S+ D +W + + + A + N
Sbjct: 107 SEIERSYYKAVTDNGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNG 166
Query: 230 FELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVT 352
E + +++L + AL K FD K K+ T+ M VT
Sbjct: 167 SERDNRKQLIS----ALDKGFDGSFKEKHFTFLQSVMMDVT 203
>sp|P55127|FRPC_NEIMC Iron-regulated protein frpC
Length = 1829
Score = 31.2 bits (69), Expect = 1.5
Identities = 24/101 (23%), Positives = 42/101 (41%)
Frame = +2
Query: 50 SDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVGQLVLGKTAEDIGNM 229
S++ YK S A L++N S+ D +W + + + A + N
Sbjct: 107 SEIERSYYKAVTYHGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNG 166
Query: 230 FELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVT 352
E + +++L + AL K FD K K+ T+ M +T
Sbjct: 167 SERDNRKQLIS----ALDKGFDGSFKEKHFTFLQSVMMDLT 203
>sp|Q9K0K9|FRPA_NEIMB Iron-regulated protein frpA
Length = 1302
Score = 30.8 bits (68), Expect = 1.9
Identities = 25/101 (24%), Positives = 42/101 (41%)
Frame = +2
Query: 50 SDLGNGLYKCERCETEGSDFAYRLILNMSIIDFTGTHWVTAFQEVGQLVLGKTAEDIGNM 229
S++ YK S A L++N S+ D +W + + + A + N
Sbjct: 107 SEIERSYYKAVTENGVSSSAAIDLVINRSLPDMADGYWALGLGIEAERIHNEQAVNNPNG 166
Query: 230 FELEQKEELENMFDEALYKDFDFKLKVKNDTYNDETRMRVT 352
E + +++L + AL K FD K K+ T+ M VT
Sbjct: 167 SERDNRKQLIS----ALDKGFDGSFKEKHFTFLQSVIMDVT 203
>sp|Q9C646|RX24L_ARATH Probable disease resistance protein RXW24L
Length = 899
Score = 30.4 bits (67), Expect = 2.5
Identities = 20/71 (28%), Positives = 38/71 (53%), Gaps = 2/71 (2%)
Frame = +2
Query: 116 RLILNMSIID--FTGTHWVTAFQEVGQLVLGKTAEDIGNMFELEQKEELENMFDEALYKD 289
R + N ++ F G WV+ QE ++ + +T + N+ E+K+E++NM + L+ D
Sbjct: 200 RQVFNHDVVKDRFDGFAWVSVSQEFTRISVWQTI--LQNLTSKERKDEIQNMKEADLHDD 257
Query: 290 FDFKLKVKNDT 322
F+L + T
Sbjct: 258 L-FRLLESSKT 267
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 59,875,922
Number of Sequences: 369166
Number of extensions: 1217972
Number of successful extensions: 3509
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3393
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3501
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 3218280650
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)