Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_L13
(380 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|O55005|ROBO1_RAT Roundabout homolog 1 precursor 52 3e-07
sp|O89026|ROBO1_MOUSE Roundabout homolog 1 precursor 52 3e-07
sp|Q9Y6N7|ROBO1_HUMAN Roundabout homolog 1 precursor (H-Rob... 52 3e-07
sp|Q96JA1|LRIG1_HUMAN Leucine-rich repeats and immunoglobul... 45 5e-05
sp|P70193|LRIG1_MOUSE Leucine-rich repeats and immunoglobul... 45 7e-05
sp|Q90773|CEPU1_CHICK CEPU-1 protein precursor 45 7e-05
sp|Q99PJ0|NTRI_MOUSE Neurotrimin precursor 44 1e-04
sp|P98160|PGBM_HUMAN Basement membrane-specific heparan sul... 44 1e-04
sp|Q9P121|NTRI_HUMAN Neurotrimin precursor (hNT) 44 1e-04
sp|Q62718|NTRI_RAT Neurotrimin precursor (GP65) 44 1e-04
>sp|O55005|ROBO1_RAT Roundabout homolog 1 precursor
Length = 1651
Score = 52.4 bits (124), Expect = 3e-07
Identities = 30/82 (36%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVN-AKAERTLMVYDLPQITYTEFKNKVEFGKDITFS 224
L+I V G G Y C + N+V A+A TL V + P V G+ +TF
Sbjct: 315 LKIRKVTAGDMGSYTC---VAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQ 371
Query: 225 CIATGFPPPSVFWKRIGGSSIL 290
C ATG P P++FW+R G ++L
Sbjct: 372 CEATGNPQPAIFWRREGSQNLL 393
Score = 38.9 bits (89), Expect = 0.004
Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
Frame = +3
Query: 66 DVGYSGIYACEVMYSANVVNAKA--ERTLMVYDLPQ--ITYTEFKNKVEFGKDITFSCIA 233
DVGY Y C+ + A + KA E T ++ D P I V +T SC+A
Sbjct: 422 DVGY---YICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLTLSCVA 478
Query: 234 TGFPPPSVFWKRIG 275
TG P P++ W++ G
Sbjct: 479 TGSPVPTILWRKDG 492
>sp|O89026|ROBO1_MOUSE Roundabout homolog 1 precursor
Length = 1612
Score = 52.4 bits (124), Expect = 3e-07
Identities = 30/82 (36%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVN-AKAERTLMVYDLPQITYTEFKNKVEFGKDITFS 224
L+I V G G Y C + N+V A+A TL V + P V G+ +TF
Sbjct: 276 LKIRKVTAGDMGSYTC---VAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQ 332
Query: 225 CIATGFPPPSVFWKRIGGSSIL 290
C ATG P P++FW+R G ++L
Sbjct: 333 CEATGNPQPAIFWRREGSQNLL 354
Score = 37.0 bits (84), Expect = 0.014
Identities = 24/74 (32%), Positives = 35/74 (47%), Gaps = 4/74 (5%)
Frame = +3
Query: 66 DVGYSGIYACEVMYSANVVNAKA--ERTLMVYDLPQ--ITYTEFKNKVEFGKDITFSCIA 233
DVGY Y C+ + A + KA E T ++ D P I V + SC+A
Sbjct: 383 DVGY---YICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTLILSCVA 439
Query: 234 TGFPPPSVFWKRIG 275
TG P P++ W++ G
Sbjct: 440 TGSPAPTILWRKDG 453
>sp|Q9Y6N7|ROBO1_HUMAN Roundabout homolog 1 precursor (H-Robo-1) (Deleted in U twenty
twenty)
Length = 1651
Score = 52.4 bits (124), Expect = 3e-07
Identities = 30/82 (36%), Positives = 42/82 (51%), Gaps = 1/82 (1%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVN-AKAERTLMVYDLPQITYTEFKNKVEFGKDITFS 224
L+I V G G Y C + N+V A+A TL V + P V G+ +TF
Sbjct: 315 LKIRKVTAGDMGSYTC---VAENMVGKAEASATLTVQEPPHFVVKPRDQVVALGRTVTFQ 371
Query: 225 CIATGFPPPSVFWKRIGGSSIL 290
C ATG P P++FW+R G ++L
Sbjct: 372 CEATGNPQPAIFWRREGSQNLL 393
Score = 37.0 bits (84), Expect = 0.014
Identities = 25/74 (33%), Positives = 36/74 (48%), Gaps = 4/74 (5%)
Frame = +3
Query: 66 DVGYSGIYACEVMYSANVVNAKA--ERTLMVYDLPQITYTEFKNKVEFGKDITF--SCIA 233
DVGY Y C+ + A + KA E T ++ D P + D TF SC+A
Sbjct: 422 DVGY---YICQTLNVAGSIITKAYLEVTDVIADRPPPVIRQGPVNQTVAVDGTFVLSCVA 478
Query: 234 TGFPPPSVFWKRIG 275
TG P P++ W++ G
Sbjct: 479 TGSPVPTILWRKDG 492
>sp|Q96JA1|LRIG1_HUMAN Leucine-rich repeats and immunoglobulin-like domains protein 1
precursor (LIG-1)
Length = 1093
Score = 45.1 bits (105), Expect = 5e-05
Identities = 23/70 (32%), Positives = 34/70 (48%)
Frame = +3
Query: 54 ILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSCIA 233
I V + +G+Y+C SA ++A A TL V + P + V G+ + C A
Sbjct: 659 ITDVKIDDAGVYSCTAQNSAGSISANA--TLTVLETPSLVVPLEDRVVSVGETVALQCKA 716
Query: 234 TGFPPPSVFW 263
TG PPP + W
Sbjct: 717 TGNPPPRITW 726
Score = 37.0 bits (84), Expect = 0.014
Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 1/79 (1%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVM-YSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFS 224
L + V G+ G Y C + + + + KA T+ V LP T T +
Sbjct: 562 LHLRQVTFGHEGRYQCVITNHFGSTYSHKARLTVNV--LPSFTKTPHDITIRTTTVARLE 619
Query: 225 CIATGFPPPSVFWKRIGGS 281
C ATG P P + W++ GG+
Sbjct: 620 CAATGHPNPQIAWQKDGGT 638
>sp|P70193|LRIG1_MOUSE Leucine-rich repeats and immunoglobulin-like domains protein 1
precursor (LIG-1)
Length = 1091
Score = 44.7 bits (104), Expect = 7e-05
Identities = 25/74 (33%), Positives = 35/74 (47%)
Frame = +3
Query: 54 ILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSCIA 233
I V + G+Y+C SA V+A A TL V + P + V G+ + F C A
Sbjct: 661 ITDVKIDDMGVYSCTAQNSAGSVSANA--TLTVLETPSLAVPLEDRVVTVGETVAFQCKA 718
Query: 234 TGFPPPSVFWKRIG 275
TG P P + W + G
Sbjct: 719 TGSPTPRITWLKGG 732
Score = 37.7 bits (86), Expect = 0.008
Identities = 23/79 (29%), Positives = 35/79 (44%), Gaps = 1/79 (1%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVM-YSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFS 224
L + V G+ G Y C + + + + KA T+ V LP T + G
Sbjct: 564 LHLRHVTFGHEGRYQCIITNHFGSTYSHKARLTVNV--LPSFTKIPHDIAIRTGTTARLE 621
Query: 225 CIATGFPPPSVFWKRIGGS 281
C ATG P P + W++ GG+
Sbjct: 622 CAATGHPNPQIAWQKDGGT 640
>sp|Q90773|CEPU1_CHICK CEPU-1 protein precursor
Length = 353
Score = 44.7 bits (104), Expect = 7e-05
Identities = 24/80 (30%), Positives = 41/80 (51%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSC 227
++I VDV G Y C V + ++ L+V P+IT T + G +++ +C
Sbjct: 98 IQIHDVDVYDEGPYTCSVQTDNHPKTSRVH--LIVQVSPKITETSSDISINEGGNVSLTC 155
Query: 228 IATGFPPPSVFWKRIGGSSI 287
IATG P P++ W+ I ++
Sbjct: 156 IATGRPDPTITWRHISPKAV 175
>sp|Q99PJ0|NTRI_MOUSE Neurotrimin precursor
Length = 344
Score = 43.9 bits (102), Expect = 1e-04
Identities = 25/80 (31%), Positives = 39/80 (48%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSC 227
+EI VDV G Y C V + ++ L+V P+I + G +I+ +C
Sbjct: 100 IEIQNVDVYDEGPYTCSVQTDNHPKTSRVH--LIVQVSPKIVEISSDISINEGNNISLTC 157
Query: 228 IATGFPPPSVFWKRIGGSSI 287
IATG P P+V W+ I ++
Sbjct: 158 IATGRPEPTVTWRHISPKAV 177
>sp|P98160|PGBM_HUMAN Basement membrane-specific heparan sulfate proteoglycan core protein
precursor (HSPG) (Perlecan) (PLC)
Length = 4391
Score = 43.9 bits (102), Expect = 1e-04
Identities = 24/78 (30%), Positives = 40/78 (51%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSC 227
+ I V++ +G Y C +A + L+V LPQI+ + + +V G F C
Sbjct: 3540 VRIAHVELADAGQYRCTATNAAGTTQSHV--LLLVQALPQISMPQ-EVRVPAGSAAVFPC 3596
Query: 228 IATGFPPPSVFWKRIGGS 281
IA+G+P P + W ++ GS
Sbjct: 3597 IASGYPTPDISWSKLDGS 3614
Score = 39.3 bits (90), Expect = 0.003
Identities = 21/77 (27%), Positives = 29/77 (37%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSC 227
L I +D G Y C+ A A+ + I V G + F C
Sbjct: 3451 LRIQNLDQSCQGTYICQAHGPWGKAQASAQLVIQALPSVLINIRTSVQTVVVGHAVEFEC 3510
Query: 228 IATGFPPPSVFWKRIGG 278
+A G P P V W ++GG
Sbjct: 3511 LALGDPKPQVTWSKVGG 3527
Score = 36.6 bits (83), Expect = 0.018
Identities = 21/79 (26%), Positives = 33/79 (41%), Gaps = 4/79 (5%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTL----MVYDLPQITYTEFKNKVEFGKDI 215
L+I + +G Y C + + E + M PQ+ E + VE G
Sbjct: 3170 LQISSAKPSDAGTYVCLAQNALGTAQKQVEVIVDTGAMAPGAPQVQAEEAELTVEAGHTA 3229
Query: 216 TFSCIATGFPPPSVFWKRI 272
T C ATG P P++ W ++
Sbjct: 3230 TLRCSATGSPAPTIHWSKL 3248
Score = 36.2 bits (82), Expect = 0.024
Identities = 22/68 (32%), Positives = 30/68 (44%)
Frame = +3
Query: 78 SGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSCIATGFPPPSV 257
SG Y C A +A+A L V P T V+ G+ + C+A G PP +
Sbjct: 3273 SGQYICNATSPAG--HAEATIILHVESPPYATTVPEHASVQAGETVQLQCLAHGTPPLTF 3330
Query: 258 FWKRIGGS 281
W R+G S
Sbjct: 3331 QWSRVGSS 3338
Score = 33.1 bits (74), Expect = 0.20
Identities = 21/88 (23%), Positives = 38/88 (43%), Gaps = 4/88 (4%)
Frame = +3
Query: 30 ETNSIRLEILTVDVGYSGIYACEVMYSANVVNAKA----ERTLMVYDLPQITYTEFKNKV 197
+T+ L + V SG Y C V+ ++ + A E +++ +P + + V
Sbjct: 2197 QTHGSLLRLHQVTPADSGEYVCHVVGTSGPLEASVLVTIEASVIPGPIPPVRIESSSSTV 2256
Query: 198 EFGKDITFSCIATGFPPPSVFWKRIGGS 281
G+ + SC+ G V W + GGS
Sbjct: 2257 AEGQTLDLSCVVAGQAHAQVTWYKRGGS 2284
Score = 32.3 bits (72), Expect = 0.34
Identities = 17/65 (26%), Positives = 30/65 (46%)
Frame = +3
Query: 87 YACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSCIATGFPPPSVFWK 266
Y C SA A+A + P++ + + +V G+ + C A G P ++ W+
Sbjct: 1931 YLCRAHSSAGQQVARAVLHVHGGGGPRVQVSPERTQVHAGRTVRLYCRAAGVPSATITWR 1990
Query: 267 RIGGS 281
+ GGS
Sbjct: 1991 KEGGS 1995
Score = 30.8 bits (68), Expect = 1.0
Identities = 23/95 (24%), Positives = 35/95 (36%), Gaps = 11/95 (11%)
Frame = +3
Query: 30 ETNSIRLEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYD-----------LPQITY 176
+T RL + V SG Y C VM S+ + A T+ P I
Sbjct: 2772 QTRGSRLRLHHVSPADSGEYVCRVMGSSGPLEASVLVTIEASGSSAVHVPAPGGAPPIRI 2831
Query: 177 TEFKNKVEFGKDITFSCIATGFPPPSVFWKRIGGS 281
++V G+ + C+ G V W + GG+
Sbjct: 2832 EPSSSRVAEGQTLDLKCVVPGQAHAQVTWHKRGGN 2866
Score = 29.6 bits (65), Expect = 2.2
Identities = 9/21 (42%), Positives = 15/21 (71%)
Frame = +3
Query: 204 GKDITFSCIATGFPPPSVFWK 266
G+ +TF+C+A G P P + W+
Sbjct: 421 GQTVTFTCVAIGVPTPIINWR 441
Score = 29.3 bits (64), Expect = 2.9
Identities = 20/68 (29%), Positives = 31/68 (45%), Gaps = 1/68 (1%)
Frame = +3
Query: 81 GIYACEVMYSANVVNAKAERTLMVYDLPQITYT-EFKNKVEFGKDITFSCIATGFPPPSV 257
G Y C + V A++ L V+ P ++ E V+ GK +T C++ G P S
Sbjct: 3087 GTYRCVASNAYGV--AQSVVNLSVHGPPTVSVLPEGPVWVKVGKAVTLECVSAGEPRSSA 3144
Query: 258 FWKRIGGS 281
W RI +
Sbjct: 3145 RWTRISST 3152
Score = 28.5 bits (62), Expect = 5.0
Identities = 19/90 (21%), Positives = 33/90 (36%), Gaps = 3/90 (3%)
Frame = +3
Query: 21 QGKETNSIRLEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYD---LPQITYTEFKN 191
+ + T+ L I + +G Y C A A+ + ++ P +
Sbjct: 2001 RSERTDIATLLIPAITTADAGFYLCVATSPAGTAQARIQVVVLSASDASPPPVKIESSSP 2060
Query: 192 KVEFGKDITFSCIATGFPPPSVFWKRIGGS 281
V G+ + +C+ G V W R GGS
Sbjct: 2061 SVTEGQTLDLNCVVAGSAHAQVTWYRRGGS 2090
Score = 28.1 bits (61), Expect = 6.5
Identities = 20/91 (21%), Positives = 36/91 (39%), Gaps = 7/91 (7%)
Frame = +3
Query: 30 ETNSIRLEILTVDVGYSGIYACEVMYSANVVNAKAERTL-------MVYDLPQITYTEFK 188
+T+ L + SG Y C V+ S+ + A T+ + P +
Sbjct: 2386 QTHGSLLRLYQASPADSGEYVCRVLGSSVPLEASVLVTIEPAGSVPALGVTPTVRIESSS 2445
Query: 189 NKVEFGKDITFSCIATGFPPPSVFWKRIGGS 281
++V G+ + +C+ G V W + GGS
Sbjct: 2446 SQVAEGQTLDLNCLVAGQAHAQVTWHKRGGS 2476
>sp|Q9P121|NTRI_HUMAN Neurotrimin precursor (hNT)
Length = 344
Score = 43.9 bits (102), Expect = 1e-04
Identities = 25/80 (31%), Positives = 39/80 (48%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSC 227
+EI VDV G Y C V + ++ L+V P+I + G +I+ +C
Sbjct: 100 IEIQNVDVYDEGPYTCSVQTDNHPKTSRVH--LIVQVSPKIVEISSDISINEGNNISLTC 157
Query: 228 IATGFPPPSVFWKRIGGSSI 287
IATG P P+V W+ I ++
Sbjct: 158 IATGRPEPTVTWRHISPKAV 177
>sp|Q62718|NTRI_RAT Neurotrimin precursor (GP65)
Length = 344
Score = 43.9 bits (102), Expect = 1e-04
Identities = 25/80 (31%), Positives = 39/80 (48%)
Frame = +3
Query: 48 LEILTVDVGYSGIYACEVMYSANVVNAKAERTLMVYDLPQITYTEFKNKVEFGKDITFSC 227
+EI VDV G Y C V + ++ L+V P+I + G +I+ +C
Sbjct: 100 IEIQNVDVYDEGPYTCSVQTDNHPKTSRVH--LIVQVSPKIVEISSDISINEGNNISLTC 157
Query: 228 IATGFPPPSVFWKRIGGSSI 287
IATG P P+V W+ I ++
Sbjct: 158 IATGRPEPTVTWRHISPKAV 177
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 43,065,314
Number of Sequences: 369166
Number of extensions: 791101
Number of successful extensions: 3032
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2400
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3007
length of database: 68,354,980
effective HSP length: 93
effective length of database: 51,174,625
effective search space used: 1688762625
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)