Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_I16
(699 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precurs... 143 4e-34
sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor... 140 2e-33
sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precurs... 138 1e-32
sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precurs... 134 3e-31
sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precurs... 133 4e-31
sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl este... 130 4e-30
sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester ... 129 1e-29
sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl este... 119 8e-27
sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1) 88 2e-17
sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3) 85 2e-16
>sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 143 bits (361), Expect = 4e-34
Identities = 70/172 (40%), Positives = 107/172 (62%), Gaps = 1/172 (0%)
Frame = -1
Query: 627 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 448
+FG ++ +FLA+ +C +E L CS +F++ G D KN N SRL VY+SH PA
Sbjct: 225 IFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPA 284
Query: 447 GTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 271
GTSV N+ H+ Q+V +G FQ YD+G N Y PP ++V + +P A+++GG D
Sbjct: 285 GTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDL 344
Query: 270 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFIKK 115
LADP+DV LL + ++ + K I +YNH+DF+W +A Q +Y D+++ I +
Sbjct: 345 LADPQDVGLLLPKL-PNLIYHKEIPFYNHLDFIWAMDAPQEVYNDIVSMISE 395
>sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Lingual lipase)
Length = 395
Score = 140 bits (354), Expect = 2e-33
Identities = 67/170 (39%), Positives = 106/170 (62%), Gaps = 1/170 (0%)
Frame = -1
Query: 627 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 448
+FG + ++ FL + +C +E L CS +F+ G D KNLN SR VY+ H PA
Sbjct: 224 MFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRFDVYLGHNPA 283
Query: 447 GTSVMNIVHYAQSVDNGNFQMYDFGIEG-NIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 271
GTSV + +H+AQ V +G FQ +++G N+ Y PP++DV + +P A+++GGND
Sbjct: 284 GTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMTVPVAVWNGGNDI 343
Query: 270 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFI 121
LADP+DV+ LL + ++ F K I YNH+DF+W +A Q +Y ++++ +
Sbjct: 344 LADPQDVAMLLPKL-SNLLFHKEILAYNHLDFIWAMDAPQEVYNEMISMM 392
>sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 138 bits (348), Expect = 1e-32
Identities = 71/187 (37%), Positives = 112/187 (59%), Gaps = 13/187 (6%)
Frame = -1
Query: 642 ETLLN------------LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDI 499
ETLLN +FG+ + +FLA+ +C +E + CS +F++ G D
Sbjct: 208 ETLLNKLMLVPSFLFKLIFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDT 267
Query: 498 KNLNESRLPVYISHTPAGTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFD 322
NLN SRL VY+SH PAGTSV N++H++Q+V +G FQ +D+G N+ Y PP ++
Sbjct: 268 MNLNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYN 327
Query: 321 VKPVGIPTAIFSGGNDTLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIY 142
+ + +P A+++GGND LADP DV LL + + +K I YNH+DF+W +A Q +Y
Sbjct: 328 LTDMHVPIAVWNGGNDLLADPHDVDLLLSKLPNLIYHRK-IPPYNHLDFIWAMDAPQAVY 386
Query: 141 PDVLNFI 121
++++ +
Sbjct: 387 NEIVSMM 393
>sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 395
Score = 134 bits (336), Expect = 3e-31
Identities = 64/170 (37%), Positives = 104/170 (61%), Gaps = 1/170 (0%)
Frame = -1
Query: 627 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 448
+FG+ M ++ + +FL + +C +E L CS +F+ G D KNLN SR VY+ H PA
Sbjct: 224 IFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRFDVYLGHNPA 283
Query: 447 GTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 271
GTS ++ H+AQ +G Q Y++G N+ Y PP +DV + +P A+++GG+D
Sbjct: 284 GTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPIAVWNGGHDI 343
Query: 270 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFI 121
LADP+DV+ LL + ++ + K I YNH+DF+W +A Q +Y +++ +
Sbjct: 344 LADPQDVAMLLPKL-PNLLYHKEILPYNHLDFIWAMDAPQEVYNEIVTMM 392
>sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Pregastric esterase) (PGE)
Length = 397
Score = 133 bits (335), Expect = 4e-31
Identities = 65/170 (38%), Positives = 103/170 (60%), Gaps = 1/170 (0%)
Frame = -1
Query: 627 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 448
+FG ++ + +FL +C +E L C +F + G D KN N SRL VYI+H PA
Sbjct: 224 IFGDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKNFNMSRLDVYIAHNPA 283
Query: 447 GTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 271
GTSV N +H+ Q+V +G FQ +D+G N+ Y P PP +++ + +P A++S ND
Sbjct: 284 GTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAMNVPIAVWSADNDL 343
Query: 270 LADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFI 121
LADP+DV LL + ++ + K I YNH+DF+W +A Q +Y ++++ +
Sbjct: 344 LADPQDVDFLLSKL-SNLIYHKEIPNYNHLDFIWAMDAPQEVYNEIVSLM 392
>sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 399
Score = 130 bits (326), Expect = 4e-30
Identities = 67/174 (38%), Positives = 107/174 (61%), Gaps = 1/174 (0%)
Frame = -1
Query: 630 NLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTP 451
+LFG + S K+L + +C L + C + FLL G + +NLN SR+ VY +H+P
Sbjct: 226 DLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSP 285
Query: 450 AGTSVMNIVHYAQSVDNGNFQMYDFGIEG-NIKRYGTPNPPQFDVKPVGIPTAIFSGGND 274
AGTSV N++H++Q+V FQ +D+G N Y PP ++VK + +PTA++SGG+D
Sbjct: 286 AGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHD 345
Query: 273 TLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFIKKY 112
LAD DV+ LL + ++ F + I + H+DF+WG +A +Y ++N ++KY
Sbjct: 346 WLADVYDVNILLTQI-TNLVFHESIPEWEHLDFIWGLDAPWRLYNKIINLMRKY 398
>sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 129 bits (323), Expect = 1e-29
Identities = 67/176 (38%), Positives = 108/176 (61%), Gaps = 1/176 (0%)
Frame = -1
Query: 636 LLNLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISH 457
L +LFG + + S + K+L++ IC + + C+ + FL+ G + KNLN SR+ VY +H
Sbjct: 222 LEDLFGQKQFLPQSAMVKWLSTHICTHVIMKELCANIFFLICGFNEKNLNMSRVDVYTTH 281
Query: 456 TPAGTSVMNIVHYAQSVDNGNFQMYDFG-IEGNIKRYGTPNPPQFDVKPVGIPTAIFSGG 280
PAGTSV N+VH+ Q V Q +D+G + N Y PP + +K + +PTA++SGG
Sbjct: 282 CPAGTSVQNMVHWTQVVKYHKLQAFDWGSSDKNYFHYNQSYPPLYSIKDMQLPTALWSGG 341
Query: 279 NDTLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFIKKY 112
D LAD D++ LL + + + K I ++H+DF+WG +A +Y +V++ +KKY
Sbjct: 342 KDWLADTSDINILLTEI-PTLVYHKNIPEWDHLDFIWGLDAPWRLYNEVVSLMKKY 396
>sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 119 bits (298), Expect = 8e-27
Identities = 62/176 (35%), Positives = 104/176 (59%), Gaps = 1/176 (0%)
Frame = -1
Query: 636 LLNLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISH 457
L ++FG + + S + K+L+ +C + + C+ V FLL G + KNLN SR+ VY +H
Sbjct: 222 LKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTH 281
Query: 456 TPAGTSVMNIVHYAQSVDNGNFQMYDFG-IEGNIKRYGTPNPPQFDVKPVGIPTAIFSGG 280
PA V N++H+ Q Q +D+G E N Y PP +++K + +PTA++SGG
Sbjct: 282 CPAELLVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGG 341
Query: 279 NDTLADPEDVSRLLGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFIKKY 112
D LAD D++ LL + + + K I ++H+DF+WG +A +Y ++++ +KKY
Sbjct: 342 RDWLADINDITILLTQI-PKLVYHKNIPEWDHLDFIWGLDAPWKLYDEIISLMKKY 396
>sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1)
Length = 439
Score = 87.8 bits (216), Expect = 2e-17
Identities = 51/169 (30%), Positives = 83/169 (49%), Gaps = 3/169 (1%)
Frame = -1
Query: 612 RVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPAGTSVM 433
R M N E F+FL E + C E +F + G + N PV + H PAG +
Sbjct: 254 REMFNGE-FRFLCRMT---EETERLCIEAVFGIVGRNWNEFNRKMFPVILGHYPAGVAAK 309
Query: 432 NIVHYAQSVDNGNFQMYDFGIEGNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDTLADPED 253
+ H+ Q + +G F Y + N++ Y PP++++ V +PT ++ ND L P+D
Sbjct: 310 QVKHFIQIIKSGRFAPYSYSSNKNMQLYRDHLPPRYNLSLVTVPTFVYYSTNDLLCHPKD 369
Query: 252 VSRL---LGLVGGDVKFQKFISYYNHMDFVWGENAWQIIYPDVLNFIKK 115
V + LG V G K+ +NHMDF+W + +++Y +L + K
Sbjct: 370 VESMCDDLGNVTG--KYLVPQKEFNHMDFLWAIDVRKMLYRRMLQVLGK 416
>sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3)
Length = 394
Score = 84.7 bits (208), Expect = 2e-16
Identities = 56/202 (27%), Positives = 99/202 (49%), Gaps = 7/202 (3%)
Frame = -1
Query: 696 LGNVEAGL-RILARFREDIETLLNLFGHGRVMSNSEIFKFLASTIC-GKEGLPKFCSEVI 523
+GN+++ L R A ++ + G M +++ + L +C C+ I
Sbjct: 196 MGNMKSPLTRAFAPILGQPNAIVEVCGSMEFMPSNKFKQDLGIEMCQATSPYADMCANEI 255
Query: 522 FLLAGHDIKNLNESRLPVYISHTPAGTSVMNIVHYAQSVDNGNFQMYDFGIEGNIKRYGT 343
FL+ G+D + L+ L + +PAG SV +H+ Q ++G F+ +D+ N YG+
Sbjct: 256 FLIGGYDTEQLDYELLEHIKATSPAGASVNQNLHFCQEYNSGKFRKFDYTALRNPYEYGS 315
Query: 342 PNPPQFDVKPVGIPTAIFSGGNDTLADPEDVSRL---LGLVGGD--VKFQKFISYYNHMD 178
PP + +K P ++ G ND + D DV +L L + D V F+K + H+D
Sbjct: 316 YFPPDYKLKNAKAPVLLYYGANDWMCDVSDVRKLRDELPNMALDYLVPFEK----WAHLD 371
Query: 177 FVWGENAWQIIYPDVLNFIKKY 112
F+WG A + +Y +VL ++ Y
Sbjct: 372 FIWGTEARKYVYDEVLKQMQSY 393
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,097,122
Number of Sequences: 369166
Number of extensions: 2029775
Number of successful extensions: 5059
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4857
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5039
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6073541875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)