Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_I07
(533 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P17139|CO4A1_CAEEL Collagen alpha 1(IV) chain precursor 60 4e-09
sp|Q01955|CO4A3_HUMAN Collagen alpha 3(IV) chain precursor ... 58 2e-08
sp|Q28084|CO4A3_BOVIN Collagen alpha 3(IV) chain 56 6e-08
sp|Q7SIB2|CO4A1_BOVIN Collagen alpha 1(IV) chain 55 8e-08
sp|P02462|CO4A1_HUMAN Collagen alpha 1(IV) chain precursor 55 1e-07
sp|P17140|CO4A2_CAEEL Collagen alpha 2(IV) chain precursor ... 54 2e-07
sp|P02463|CO4A1_MOUSE Collagen alpha 1(IV) chain precursor 54 2e-07
sp|P29400|CO4A5_HUMAN Collagen alpha 5(IV) chain precursor 52 9e-07
sp|P27393|CO4A2_ASCSU Collagen alpha 2(IV) chain precursor 52 9e-07
sp|Q28247|CO4A5_CANFA Collagen alpha 5(IV) chain precursor 52 1e-06
>sp|P17139|CO4A1_CAEEL Collagen alpha 1(IV) chain precursor
Length = 1759
Score = 59.7 bits (143), Expect = 4e-09
Identities = 26/50 (52%), Positives = 31/50 (62%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G FA HSQ+T +P CP LW+GYS L + G+ RA DL PGSCL
Sbjct: 1535 GFTFAKHSQTTAVPQCPPGASQLWEGYSLLYVQGNGRASGQDLGQPGSCL 1584
Score = 43.9 bits (102), Expect = 2e-04
Identities = 23/53 (43%), Positives = 29/53 (54%), Gaps = 5/53 (9%)
Frame = +3
Query: 390 IFATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
I A HSQ T +P CP +W GYSF+ G G+ ++ L SPGSCL
Sbjct: 1647 IIAVHSQDTSVPQCPQGWSGMWTGYSFVMHTAAGAEGTGQS----LQSPGSCL 1695
>sp|Q01955|CO4A3_HUMAN Collagen alpha 3(IV) chain precursor (Goodpasture antigen)
Length = 1670
Score = 57.8 bits (138), Expect = 2e-08
Identities = 26/50 (52%), Positives = 33/50 (66%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G +F HSQ+T IP CP T L+ G+SFL + G+ RAH DL + GSCL
Sbjct: 1445 GFVFTRHSQTTAIPSCPEGTVPLYSGFSFLFVQGNQRAHGQDLGTLGSCL 1494
Score = 43.9 bits (102), Expect = 2e-04
Identities = 24/51 (47%), Positives = 31/51 (60%), Gaps = 5/51 (9%)
Frame = +3
Query: 396 ATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
A HSQ+T IP CP LW+G+SF+ G G+ +A L+SPGSCL
Sbjct: 1559 AVHSQTTDIPPCPHGWISLWKGFSFIMFTSAGSEGTGQA----LASPGSCL 1605
>sp|Q28084|CO4A3_BOVIN Collagen alpha 3(IV) chain
Length = 471
Score = 55.8 bits (133), Expect = 6e-08
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G +F HSQ+T IP CP T L+ G+S L + G+++AH DL + GSCL
Sbjct: 246 GFVFTRHSQTTAIPSCPEGTEPLYSGFSLLFVQGNEQAHGQDLGTLGSCL 295
Score = 44.7 bits (104), Expect = 1e-04
Identities = 24/51 (47%), Positives = 32/51 (62%), Gaps = 5/51 (9%)
Frame = +3
Query: 396 ATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
A HSQ+T IP CP+ LW+G+SF+ G G+ +A L+SPGSCL
Sbjct: 360 AVHSQTTDIPPCPAGWISLWKGFSFIMFTSAGSEGAGQA----LASPGSCL 406
>sp|Q7SIB2|CO4A1_BOVIN Collagen alpha 1(IV) chain
Length = 229
Score = 55.5 bits (132), Expect = 8e-08
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ+T P CP T++L+ GYS L + G++RAH DL + GSCL
Sbjct: 5 GFLVTRHSQTTDDPQCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCL 54
Score = 46.2 bits (108), Expect = 5e-05
Identities = 25/53 (47%), Positives = 32/53 (60%), Gaps = 5/53 (9%)
Frame = +3
Query: 390 IFATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
+ A HSQ+ +IP CP+ LW GYSF+ G GS +A L+SPGSCL
Sbjct: 117 VMAVHSQTIQIPQCPTGWSSLWIGYSFVMHTSAGAEGSGQA----LASPGSCL 165
>sp|P02462|CO4A1_HUMAN Collagen alpha 1(IV) chain precursor
Length = 1669
Score = 55.1 bits (131), Expect = 1e-07
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ+ P CPS T++L+ GYS L + G++RAH DL + GSCL
Sbjct: 1445 GFLVTRHSQTIDDPQCPSGTKILYHGYSLLYVQGNERAHGQDLGTAGSCL 1494
Score = 47.0 bits (110), Expect = 3e-05
Identities = 26/53 (49%), Positives = 32/53 (60%), Gaps = 5/53 (9%)
Frame = +3
Query: 390 IFATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
+ A HSQ+ +IP CPS LW GYSF+ G GS +A L+SPGSCL
Sbjct: 1557 VMAVHSQTIQIPPCPSGWSSLWIGYSFVMHTSAGAEGSGQA----LASPGSCL 1605
>sp|P17140|CO4A2_CAEEL Collagen alpha 2(IV) chain precursor (Lethal protein 2)
Length = 1758
Score = 54.3 bits (129), Expect = 2e-07
Identities = 22/50 (44%), Positives = 31/50 (62%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ+T++P CP LW GYS L + G++++H DL GSCL
Sbjct: 1531 GFVLVKHSQTTEVPRCPEGQTKLWDGYSLLYIEGNEKSHNQDLGHAGSCL 1580
Score = 42.0 bits (97), Expect = 0.001
Identities = 22/47 (46%), Positives = 27/47 (57%), Gaps = 1/47 (2%)
Frame = +3
Query: 396 ATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSD-RAHVNDLSSPGSCL 533
A HSQ+ +IP+CP+ LW GYSF G+ SSPGSCL
Sbjct: 1643 AVHSQTIQIPNCPAGWSSLWIGYSFAMHTGAGAEGGGQSPSSPGSCL 1689
>sp|P02463|CO4A1_MOUSE Collagen alpha 1(IV) chain precursor
Length = 1669
Score = 53.9 bits (128), Expect = 2e-07
Identities = 24/50 (48%), Positives = 33/50 (66%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ+T P CP T++L+ GYS L + G++RAH DL + GSCL
Sbjct: 1445 GFLVTRHSQTTDDPLCPPGTKILYHGYSLLYVQGNERAHGQDLGTAGSCL 1494
Score = 46.2 bits (108), Expect = 5e-05
Identities = 25/53 (47%), Positives = 32/53 (60%), Gaps = 5/53 (9%)
Frame = +3
Query: 390 IFATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
+ A HSQ+ +IP CP+ LW GYSF+ G GS +A L+SPGSCL
Sbjct: 1557 VMAVHSQTIQIPQCPNGWSSLWIGYSFVMHTSAGAEGSGQA----LASPGSCL 1605
>sp|P29400|CO4A5_HUMAN Collagen alpha 5(IV) chain precursor
Length = 1685
Score = 52.0 bits (123), Expect = 9e-07
Identities = 22/50 (44%), Positives = 31/50 (62%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ+T P CP T +++G+S L + G+ RAH DL + GSCL
Sbjct: 1461 GFLITRHSQTTDAPQCPQGTLQVYEGFSLLYVQGNKRAHGQDLGTAGSCL 1510
Score = 45.4 bits (106), Expect = 9e-05
Identities = 25/53 (47%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +3
Query: 390 IFATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
+ A HSQ+ +IP CP LW GYSF+ G GS +A L+SPGSCL
Sbjct: 1573 VIAVHSQTIQIPHCPQGWDSLWIGYSFMMHTSAGAEGSGQA----LASPGSCL 1621
>sp|P27393|CO4A2_ASCSU Collagen alpha 2(IV) chain precursor
Length = 1763
Score = 52.0 bits (123), Expect = 9e-07
Identities = 21/50 (42%), Positives = 31/50 (62%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ++++P CP LW GYS L + G++++H DL GSCL
Sbjct: 1533 GFLLVKHSQTSEVPQCPPGMVKLWDGYSLLYIEGNEKSHNQDLGHAGSCL 1582
Score = 46.6 bits (109), Expect = 4e-05
Identities = 23/50 (46%), Positives = 30/50 (60%), Gaps = 1/50 (2%)
Frame = +3
Query: 387 SIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSD-RAHVNDLSSPGSCL 533
++ A HSQ+ +IP+CP+ LW GYSF G+ LSSPGSCL
Sbjct: 1642 NVIAVHSQTIQIPNCPNGWNSLWIGYSFAMHTGAGAEGGGQSLSSPGSCL 1691
>sp|Q28247|CO4A5_CANFA Collagen alpha 5(IV) chain precursor
Length = 1691
Score = 51.6 bits (122), Expect = 1e-06
Identities = 22/50 (44%), Positives = 31/50 (62%)
Frame = +3
Query: 384 GSIFATHSQSTKIPDCPSKTRMLWQGYSFLGMMGSDRAHVNDLSSPGSCL 533
G + HSQ+T P CP T +++G+S L + G+ RAH DL + GSCL
Sbjct: 1467 GFLITRHSQTTDAPQCPHGTVQIYEGFSLLYVQGNKRAHGQDLGTAGSCL 1516
Score = 45.1 bits (105), Expect = 1e-04
Identities = 25/53 (47%), Positives = 31/53 (58%), Gaps = 5/53 (9%)
Frame = +3
Query: 390 IFATHSQSTKIPDCPSKTRMLWQGYSFL-----GMMGSDRAHVNDLSSPGSCL 533
+ A HSQ+ +IP CP LW GYSF+ G GS +A L+SPGSCL
Sbjct: 1579 VIAVHSQTIQIPHCPHGWDSLWIGYSFMMHTSAGAEGSGQA----LASPGSCL 1627
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,341,732
Number of Sequences: 369166
Number of extensions: 815672
Number of successful extensions: 2937
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2791
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2922
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3650218350
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)