Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_H14
(810 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9FI53|FUM2_ARATH Fumarate hydratase 2, chloroplast prec... 286 6e-77
sp|P93033|FUM1_ARATH Fumarate hydratase 1, mitochondrial pr... 285 1e-76
sp|Q60HF9|FUMH_MACFA Fumarate hydratase, mitochondrial prec... 284 2e-76
sp|P14408|FUMH_RAT Fumarate hydratase, mitochondrial precur... 284 2e-76
sp|P07954|FUMH_HUMAN Fumarate hydratase, mitochondrial prec... 283 4e-76
sp|P97807|FUMH_MOUSE Fumarate hydratase, mitochondrial prec... 283 5e-76
sp|P10173|FUMH_PIG Fumarate hydratase, mitochondrial (Fumar... 278 1e-74
sp|P55250|FUMH_RHIOR Fumarate hydratase, mitochondrial prec... 278 1e-74
sp|Q92GW0|FUMC_RICCN Fumarate hydratase class II (Fumarase C) 278 1e-74
sp|Q9ZCQ4|FUMC_RICPR Fumarate hydratase class II (Fumarase C) 276 4e-74
>sp|Q9FI53|FUM2_ARATH Fumarate hydratase 2, chloroplast precursor (Fumarase 2)
Length = 499
Score = 286 bits (731), Expect = 6e-77
Identities = 143/269 (53%), Positives = 186/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EF ++KIGRTH QDA PLT+GQE Y +Q++Y +NR++ ++ QLA
Sbjct: 209 KSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVEYGLNRVACTLP-RIYQLAQGGTAV 267
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
FDV++ + E F A+NKF L AHD+ + +G LNT+AT
Sbjct: 268 GTGLNTKKGFDVKIAAAVAEETN------LPFVTAENKFEALAAHDACVETSGSLNTIAT 321
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPRCG+GE LPENEPGSSIMPGK+NPTQCE+++MVCAQVMGN+VA
Sbjct: 322 SLMKIANDIRFLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 381
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
++IG S G+ ELNV+KP+I + LLHSI+L+AD S F K CV G+ N +I+ +++SL
Sbjct: 382 VTIGGSNGHFELNVFKPVIASALLHSIRLIADASASFEKNCVRGIEANRERISKLLHESL 441
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVT+LN IGYD +A +AK AHK C+L
Sbjct: 442 MLVTSLNPKIGYDNAAAVAKRAHKEGCTL 470
>sp|P93033|FUM1_ARATH Fumarate hydratase 1, mitochondrial precursor (Fumarase 1)
Length = 492
Score = 285 bits (729), Expect = 1e-76
Identities = 141/269 (52%), Positives = 186/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EF ++KIGRTH QDA PLT+GQE Y +Q+ Y +NR++ ++ QLA
Sbjct: 202 KSFEFKDIVKIGRTHTQDATPLTLGQEFGGYATQVKYGLNRVTCTLP-RLYQLAQGGTAV 260
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
FDV++ + E F A+NKF L AHD+ + +G LNT+AT
Sbjct: 261 GTGLNTKKGFDVKIAAAVAEETN------LPFVTAENKFEALAAHDACVETSGSLNTIAT 314
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPRCG+GE +LPENEPGSSIMPGK+NPTQCE+++MVCAQVMGN+VA
Sbjct: 315 SLMKIANDIRFLGSGPRCGLGELVLPENEPGSSIMPGKVNPTQCEALTMVCAQVMGNHVA 374
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
+++G S G+ ELNV+KP+I + LLHS++L+AD S F K CV G+ N +I+ +++SL
Sbjct: 375 VTVGGSNGHFELNVFKPVIASALLHSVRLIADASASFEKNCVRGIEANRERISKLLHESL 434
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVT+LN IGYD +A +AK AHK C+L
Sbjct: 435 MLVTSLNPKIGYDNAAAVAKKAHKEGCTL 463
>sp|Q60HF9|FUMH_MACFA Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 510
Score = 284 bits (726), Expect = 2e-76
Identities = 146/269 (54%), Positives = 187/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EFA++IKIGRTH QDAVPLT+GQE S Y+ Q+ Y + RI ++ +LA
Sbjct: 221 KSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAVTRIKAAMP-RIYELAAGGTAV 279
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
F +V ++ F A NKF L AHD+L+ ++G +NT A
Sbjct: 280 GTGLNTRIGFAEKVAAKVAALTG------LPFVTAPNKFEALAAHDALVELSGAMNTTAC 333
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA
Sbjct: 334 SLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVA 393
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
+++G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SL
Sbjct: 394 VTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESL 453
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYDK+A+IAK AHKN +L
Sbjct: 454 MLVTALNPHIGYDKAAKIAKTAHKNGSTL 482
>sp|P14408|FUMH_RAT Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 507
Score = 284 bits (726), Expect = 2e-76
Identities = 147/269 (54%), Positives = 187/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EFA+VIKIGRTH QDAVPLT+GQE S Y+ Q+ Y + RI ++ +LA
Sbjct: 218 KSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMERIKAAMP-RIYELAAGGTAV 276
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
F +V ++ F A NKF L AHD+L+ ++G +NT A
Sbjct: 277 GTGLNTRIGFAEKVAAKVAALTG------LPFVTAPNKFEALAAHDALVELSGAMNTTAC 330
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA
Sbjct: 331 SLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVA 390
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
+++G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SL
Sbjct: 391 VTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESL 450
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYDK+A+IAK AHKN +L
Sbjct: 451 MLVTALNPHIGYDKAAKIAKTAHKNGSTL 479
>sp|P07954|FUMH_HUMAN Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 510
Score = 283 bits (724), Expect = 4e-76
Identities = 146/269 (54%), Positives = 187/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EFA++IKIGRTH QDAVPLT+GQE S Y+ Q+ Y + RI ++ +LA
Sbjct: 221 KSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMP-RIYELAAGGTAV 279
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
F +V ++ F A NKF L AHD+L+ ++G +NT A
Sbjct: 280 GTGLNTRIGFAEKVAAKVAALTG------LPFVTAPNKFEALAAHDALVELSGAMNTTAC 333
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA
Sbjct: 334 SLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVA 393
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
+++G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SL
Sbjct: 394 VTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESL 453
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYDK+A+IAK AHKN +L
Sbjct: 454 MLVTALNPHIGYDKAAKIAKTAHKNGSTL 482
>sp|P97807|FUMH_MOUSE Fumarate hydratase, mitochondrial precursor (Fumarase) (EF-3)
Length = 507
Score = 283 bits (723), Expect = 5e-76
Identities = 147/269 (54%), Positives = 187/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EFA+VIKIGRTH QDAVPLT+GQE S Y+ Q+ Y + RI ++ +LA
Sbjct: 218 KSKEFAQVIKIGRTHTQDAVPLTLGQEFSGYVQQVQYAMVRIKAAMP-RIYELAAGGTAV 276
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
F +V ++ F A NKF L AHD+L+ ++G +NT A
Sbjct: 277 GTGLNTRIGFAEKVAAKVAALTG------LPFVTAPNKFEALAAHDALVELSGAMNTAAC 330
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+M+MV AQVMGN+VA
Sbjct: 331 SLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVA 390
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
+++G S G+ ELNV+KP++I N+LHS +LL D S F + CV+G+ NT +IN +N+SL
Sbjct: 391 VTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESL 450
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYDK+A+IAK AHKN +L
Sbjct: 451 MLVTALNPHIGYDKAAKIAKTAHKNGSTL 479
>sp|P10173|FUMH_PIG Fumarate hydratase, mitochondrial (Fumarase)
Length = 466
Score = 278 bits (712), Expect = 1e-74
Identities = 144/269 (53%), Positives = 185/269 (68%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EFA++IKIGRTH QDAVPLT+GQE S Y+ Q+ Y I RI ++ +LA
Sbjct: 177 KSREFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAITRIKAAMP-RIYELAAGGTAV 235
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
F +V ++ F A N F L AHD+L+ +G +NT A
Sbjct: 236 GTGLNTRIGFAEKVAAKVAALTG------LPFVTAPNNFEALAAHDALVEHSGAMNTTAC 289
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND RFLGSGPR G+GE +LPENEPGSSIMPGK+NPTQCE+++MV AQVMGN+VA
Sbjct: 290 SLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEALTMVAAQVMGNHVA 349
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
+++G S G+ ELNV+KP++I N+LHS +LL D + F + CV+G+ NT +IN +N+SL
Sbjct: 350 VTVGGSNGHFELNVFKPMMIKNVLHSARLLGDAAVSFTENCVVGIQANTERINKLMNESL 409
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYDK+A+IAK AHKN +L
Sbjct: 410 MLVTALNPHIGYDKAAKIAKTAHKNGSTL 438
>sp|P55250|FUMH_RHIOR Fumarate hydratase, mitochondrial precursor (Fumarase)
Length = 494
Score = 278 bits (711), Expect = 1e-74
Identities = 145/269 (53%), Positives = 180/269 (66%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K EF +IKIGRTH+QDA PLT+GQE S Y QL Y I R+ +++ LA
Sbjct: 204 KSAEFEHIIKIGRTHLQDATPLTLGQEFSGYTQQLTYGIARVQGTLE-RLYNLAQGGTAV 262
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
FD +V + I F A NKF L AHD+L+ G LNT+A
Sbjct: 263 GTGLNTRKGFDAKVAEAIASITG------LPFKTAPNKFEALAAHDALVEAHGALNTVAC 316
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND R+LGSGPRCG+GE LPENEPGSSIMPGK+NPTQCE+M+MVCAQVMGN A
Sbjct: 317 SLMKIANDIRYLGSGPRCGLGELSLPENEPGSSIMPGKVNPTQCEAMTMVCAQVMGNNTA 376
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
IS+ S G ELNV+KP++I NL+ SI+L++D S F K CV+G+ N +I++ +N+SL
Sbjct: 377 ISVAGSNGQFELNVFKPVMIKNLIQSIRLISDASISFTKNCVVGIEANEKKISSIMNESL 436
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYDK+A+ AK AHK +L
Sbjct: 437 MLVTALNPHIGYDKAAKCAKKAHKEGTTL 465
>sp|Q92GW0|FUMC_RICCN Fumarate hydratase class II (Fumarase C)
Length = 463
Score = 278 bits (711), Expect = 1e-74
Identities = 144/269 (53%), Positives = 189/269 (70%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K ++ K+IKIGRTH+QDA PLT+ QE S YI+Q++Y + RI N+++ LA
Sbjct: 174 KSKDWDKIIKIGRTHLQDATPLTLKQEFSGYITQIEYALERIENALQ-KVYLLAQGGTAV 232
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
FD++ +++ E K+ F A NKF L AHD+L+ +G LNT+A
Sbjct: 233 GTGINSKIGFDIKFAEKVAEFTKQ------PFKTAPNKFESLAAHDALVEFSGTLNTIAV 286
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND R LGSGPRCG+GE LPENEPGSSIMPGK+NPTQ E+++MVC+QVMGN+V
Sbjct: 287 SLMKIANDIRLLGSGPRCGLGELHLPENEPGSSIMPGKVNPTQVEALTMVCSQVMGNHVT 346
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
++I S G+LELNV+KP+II N+L SI+LL+D FV +CV GL N A+IN ++SL
Sbjct: 347 VTIAGSNGHLELNVFKPVIIYNILQSIELLSDSVNSFVTHCVKGLEPNIARINDLRDKSL 406
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVTALN HIGYD +A+IAK AHK+ +L
Sbjct: 407 MLVTALNPHIGYDNAAKIAKEAHKHGITL 435
>sp|Q9ZCQ4|FUMC_RICPR Fumarate hydratase class II (Fumarase C)
Length = 461
Score = 276 bits (707), Expect = 4e-74
Identities = 144/269 (53%), Positives = 187/269 (69%)
Frame = +2
Query: 2 KKLEFAKVIKIGRTHMQDAVPLTVGQELSSYISQLDYNINRISNSVKIAAKQLAIXXXXX 181
K ++ K+IKIGRTH+QDA PLT+ QE S YI+Q++Y + RI +++K LA
Sbjct: 174 KSKDWDKIIKIGRTHLQDATPLTLKQEFSGYITQIEYALERIEDALK-KVYLLAQGGTAV 232
Query: 182 XXXXXXXXXFDVEVCQRIKENVKKYLNVEADFGPAQNKFSVLGAHDSLMSVAGDLNTLAT 361
FD++ Q++ E ++ F A NKF L AHD+L+ +G LNT+A
Sbjct: 233 GTGINSKIGFDIKFAQKVAEFTQQ------PFKTAPNKFESLAAHDALVEFSGTLNTIAV 286
Query: 362 SLMKIANDFRFLGSGPRCGVGEFLLPENEPGSSIMPGKINPTQCESMSMVCAQVMGNYVA 541
SLMKIAND R LGSGPRCG+GE LPENEPGSSIMPGK+NPTQ E+++MVC QVMGN+V
Sbjct: 287 SLMKIANDIRLLGSGPRCGLGELHLPENEPGSSIMPGKVNPTQVEALTMVCTQVMGNHVT 346
Query: 542 ISIGSSQGNLELNVYKPLIINNLLHSIQLLADCSKCFVKYCVLGLTLNTAQINTYVNQSL 721
++I S G+LELNV+KP+II N+L SI+LL+D FV +CV GL N A+INT ++SL
Sbjct: 347 VTIAGSNGHLELNVFKPVIIYNILQSIELLSDSVNSFVTHCVKGLEPNIARINTLRDKSL 406
Query: 722 MLVTALNSHIGYDKSAQIAKYAHKNNCSL 808
MLVT LN HIGYD +A+IAK AHK +L
Sbjct: 407 MLVTVLNPHIGYDNAAKIAKEAHKYGITL 435
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 91,010,787
Number of Sequences: 369166
Number of extensions: 1840607
Number of successful extensions: 5123
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5007
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7715018400
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)