Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_H02
(625 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9Y697|NFS1_HUMAN Cysteine desulfurase, mitochondrial pr... 271 8e-73
sp|Q99P39|NFS1_RAT Cysteine desulfurase, mitochondrial prec... 270 3e-72
sp|Q9Z1J3|NFS1_MOUSE Cysteine desulfurase, mitochondrial pr... 267 2e-71
sp|Q9VKD3|NFS1_DROME Probable cysteine desulfurase, mitocho... 262 6e-70
sp|O60028|NFS1_ASHGO Cysteine desulfurase, mitochondrial pr... 224 2e-58
sp|O49543|NFS1_ARATH Cysteine desulfurase, mitochondrial pr... 222 5e-58
sp|P25374|NFS1_YEAST Cysteine desulfurase, mitochondrial pr... 222 5e-58
sp|O74351|NFS1_SCHPO Probable cysteine desulfurase, mitocho... 222 5e-58
sp|P87185|NFS1_CANAL Cysteine desulfurase, mitochondrial pr... 218 1e-56
sp|P87187|NFS1_CANMA Cysteine desulfurase, mitochondrial pr... 214 1e-55
>sp|Q9Y697|NFS1_HUMAN Cysteine desulfurase, mitochondrial precursor (HUSSY-08)
Length = 457
Score = 271 bits (694), Expect = 8e-73
Identities = 131/179 (73%), Positives = 151/179 (84%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
E +QSGGGQERGMRSGT+P+ L VGLGAACE+A+ E+EYD +RI +LS RL I L
Sbjct: 279 EALQSGGGQERGMRSGTVPTPLVVGLGAACEVAQQEMEYDHKRISKLSERLIQNIMKSLP 338
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
+V NGDP + YPGCIN+SF +VEGESLLM LKDVALSSGSACTS SLEPSYVLRAIG
Sbjct: 339 DVVMNGDPKHHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTD 398
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
EDLAHSSIRFGIGRFTT EE+DYTV+KC++ VK LR MSPLWEMV++GID+ +IKWTQH
Sbjct: 399 EDLAHSSIRFGIGRFTTEEEVDYTVEKCIQHVKRLREMSPLWEMVQDGIDLKSIKWTQH 457
>sp|Q99P39|NFS1_RAT Cysteine desulfurase, mitochondrial precursor
Length = 451
Score = 270 bits (689), Expect = 3e-72
Identities = 131/179 (73%), Positives = 149/179 (83%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
E +QSGGGQERGMRSGT+P+ L VGLGAACELA+ E+EYD +RI +L+ RL I L
Sbjct: 273 EALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLIQKIMKNLP 332
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
+V NGDP YPGCIN+SF +VEGESLLM LKDVALSSGSACTS SLEPSYVLRAIG
Sbjct: 333 DVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTD 392
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
EDLAHSSIRFGIGRFTT EE+DYTV+KC+ VK LR MSPLWEMV++GID+ +IKWTQH
Sbjct: 393 EDLAHSSIRFGIGRFTTEEEVDYTVQKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451
>sp|Q9Z1J3|NFS1_MOUSE Cysteine desulfurase, mitochondrial precursor (m-Nfs1)
Length = 451
Score = 267 bits (682), Expect = 2e-71
Identities = 129/179 (72%), Positives = 147/179 (82%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
E +QSGGGQERGMRSGT+P+ L VGLGAACELA+ E+EYD +RI +L+ RL I L
Sbjct: 273 EALQSGGGQERGMRSGTVPTPLVVGLGAACELAQQEMEYDHKRISKLAERLVQKIMKNLP 332
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
+V NGDP YPGCIN+SF +VEGESLLM LKDVALSSGSACTS SLEPSYV RAIG
Sbjct: 333 DVVMNGDPKQHYPGCINLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVFRAIGTD 392
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
EDLAHSSIRFGIGRFTT EE+DYT +KC+ VK LR MSPLWEMV++GID+ +IKWTQH
Sbjct: 393 EDLAHSSIRFGIGRFTTEEEVDYTAEKCIHHVKRLREMSPLWEMVQDGIDLKSIKWTQH 451
>sp|Q9VKD3|NFS1_DROME Probable cysteine desulfurase, mitochondrial precursor
Length = 462
Score = 262 bits (669), Expect = 6e-70
Identities = 128/179 (71%), Positives = 150/179 (83%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
EPIQSGGGQERG+RSGT+P+ L VGLGAA EL+ E++YDK+ + LSNRL D IS+ L
Sbjct: 284 EPIQSGGGQERGLRSGTVPAPLAVGLGAAAELSLREMDYDKKWVDFLSNRLLDRISSALP 343
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
H++ NGD Y GC+N+SF +VEGESLLM LKDVALSSGSACTS SLEPSYVLRAIG
Sbjct: 344 HVIRNGDAKATYNGCLNLSFAYVEGESLLMALKDVALSSGSACTSASLEPSYVLRAIGTD 403
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
EDLAHSSIRFGIGRFTTVEE+DYT KC++ V+ LR MSPLWEMV+EGID+ TI+W+QH
Sbjct: 404 EDLAHSSIRFGIGRFTTVEEVDYTADKCIKHVERLREMSPLWEMVQEGIDLKTIQWSQH 462
>sp|O60028|NFS1_ASHGO Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 490
Score = 224 bits (570), Expect = 2e-58
Identities = 110/179 (61%), Positives = 135/179 (75%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
EP+ SGGGQERG RSGTLP L VGLG A +L E EYD + +LS+RL G+ + ++
Sbjct: 313 EPLLSGGGQERGFRSGTLPPPLVVGLGHAAKLMVEEYEYDSAHVRRLSDRLLKGLLS-IE 371
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
NG + YPGC+N+SF FVEGESLLM L+D+ALSSGSACTS SLEPSYVL AIG
Sbjct: 372 QTTLNGSADHRYPGCVNVSFAFVEGESLLMALRDIALSSGSACTSASLEPSYVLHAIGRD 431
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
+ LAHSSIRFGIGRFTT E+DY +K E+V+ LR +SPLWEM ++GID+NTI+W+ H
Sbjct: 432 DALAHSSIRFGIGRFTTEAEVDYVIKAITERVEFLRELSPLWEMYKDGIDLNTIEWSGH 490
>sp|O49543|NFS1_ARATH Cysteine desulfurase, mitochondrial precursor
Length = 453
Score = 222 bits (566), Expect = 5e-58
Identities = 112/179 (62%), Positives = 140/179 (78%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
EP+ +GGGQERG+RSGT + VG GAACELA E+EYD++ I L RL +G+ +LD
Sbjct: 275 EPLMNGGGQERGLRSGTGATQQIVGFGAACELAMKEMEYDEKWIKGLQERLLNGVREKLD 334
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
+V NG + Y G +N+SF +VEGESLLM LK+VA+SSGSACTS SLEPSYVLRA+G
Sbjct: 335 GVVVNGSMDSRYVGNLNLSFAYVEGESLLMGLKEVAVSSGSACTSASLEPSYVLRALGVD 394
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
ED+AH+SIRFGIGRFTT EEID V+ V++V+ LR MSPL+EMV+EGIDI I+W+QH
Sbjct: 395 EDMAHTSIRFGIGRFTTKEEIDKAVELTVKQVEKLREMSPLYEMVKEGIDIKNIQWSQH 453
>sp|P25374|NFS1_YEAST Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 497
Score = 222 bits (566), Expect = 5e-58
Identities = 108/179 (60%), Positives = 136/179 (75%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
EP+ SGGGQERG+RSGTL L G G A L + E + D+ I +LS++L G+ + +
Sbjct: 320 EPLLSGGGQERGLRSGTLAPPLVAGFGEAARLMKKEFDNDQAHIKRLSDKLVKGLLSA-E 378
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
H NG P + YPGC+N+SF +VEGESLLM L+D+ALSSGSACTS SLEPSYVL A+G
Sbjct: 379 HTTLNGSPDHRYPGCVNVSFAYVEGESLLMALRDIALSSGSACTSASLEPSYVLHALGKD 438
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
+ LAHSSIRFGIGRF+T EE+DY VK ++VK LR +SPLWEMV+EGID+N+IKW+ H
Sbjct: 439 DALAHSSIRFGIGRFSTEEEVDYVVKAVSDRVKFLRELSPLWEMVQEGIDLNSIKWSGH 497
>sp|O74351|NFS1_SCHPO Probable cysteine desulfurase, mitochondrial precursor
Length = 498
Score = 222 bits (566), Expect = 5e-58
Identities = 108/179 (60%), Positives = 137/179 (76%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
EP+ SGGGQERG+RSGTL + VG G A + + E++YD I +LS RL DG+ +
Sbjct: 321 EPLISGGGQERGLRSGTLAPSQVVGFGTAARICKEEMKYDYAHISKLSQRLIDGLL-AIP 379
Query: 183 HIVFNGDPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIGAK 362
+ NGDP + YPGC+NISF +VEGESLLM LK++ALSSGSACTS SLEPSYVLRAIG
Sbjct: 380 YTSLNGDPKSRYPGCVNISFNYVEGESLLMGLKNIALSSGSACTSASLEPSYVLRAIGQS 439
Query: 363 EDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQH 539
++ AHSSIRFGIGRFTT EIDY ++ +V LR MSPLW++V+EG+D++TI+W+QH
Sbjct: 440 DENAHSSIRFGIGRFTTEAEIDYAIENVSRQVSFLRNMSPLWDLVQEGVDLSTIEWSQH 498
>sp|P87185|NFS1_CANAL Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 488
Score = 218 bits (555), Expect = 1e-56
Identities = 109/181 (60%), Positives = 137/181 (75%), Gaps = 2/181 (1%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
+PI +GGGQERG+RSGTL L G G A L + E +DK I +LS++LK+G+ + +
Sbjct: 309 DPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESSFDKRHIEKLSSKLKNGLLS-IP 367
Query: 183 HIVFNG--DPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIG 356
FNG D + YPGC+N+SF ++EGESLLM LKD+ALSSGSACTS SLEPSYVL A+G
Sbjct: 368 STQFNGCNDAKSQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVLHALG 427
Query: 357 AKEDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQ 536
A + LAHSSIRFGIGRFTT E+DY ++ E+V LR MSPLWEMV+EGID+NTI+W+
Sbjct: 428 ADDALAHSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQEGIDLNTIEWSG 487
Query: 537 H 539
H
Sbjct: 488 H 488
>sp|P87187|NFS1_CANMA Cysteine desulfurase, mitochondrial precursor (tRNA splicing
protein SPL1)
Length = 484
Score = 214 bits (545), Expect = 1e-55
Identities = 107/181 (59%), Positives = 136/181 (75%), Gaps = 2/181 (1%)
Frame = +3
Query: 3 EPIQSGGGQERGMRSGTLPSTLCVGLGAACELAESELEYDKERIIQLSNRLKDGISNQLD 182
+PI +GGGQERG+RSGTL L G G A L + E +DK+ I +LS +LK+G+ + +
Sbjct: 305 DPIITGGGQERGLRSGTLAPPLVAGFGEAARLMKQESAFDKKHIEKLSTKLKNGLLS-IP 363
Query: 183 HIVFNG--DPINGYPGCINISFGFVEGESLLMQLKDVALSSGSACTSQSLEPSYVLRAIG 356
FNG +P YPGC+N+SF ++EGESLLM LKD+ALSSGSACTS SLEPSYVL A+G
Sbjct: 364 STQFNGCNNPTYQYPGCVNVSFAYIEGESLLMALKDIALSSGSACTSASLEPSYVLHALG 423
Query: 357 AKEDLAHSSIRFGIGRFTTVEEIDYTVKKCVEKVKHLRTMSPLWEMVEEGIDINTIKWTQ 536
A + LAHSSIRFGIGRFTT E+DY ++ E+V LR MSPLWEMV+ GID+N+I+W+
Sbjct: 424 ADDALAHSSIRFGIGRFTTEAEVDYVIQAINERVDFLRKMSPLWEMVQGGIDLNSIEWSG 483
Query: 537 H 539
H
Sbjct: 484 H 484
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 65,910,611
Number of Sequences: 369166
Number of extensions: 1262436
Number of successful extensions: 3348
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3249
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3296
length of database: 68,354,980
effective HSP length: 106
effective length of database: 48,773,070
effective search space used: 4926080070
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)