Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_D09
(684 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic 281 9e-76
sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic 279 4e-75
sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic 279 6e-75
sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic 278 7e-75
sp|Q83C87|MDH_COXBU Malate dehydrogenase 240 3e-63
sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2 233 3e-61
sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1 233 4e-61
sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic 232 6e-61
sp|P10584|MDH_THETH Malate dehydrogenase >gi|62286973|sp|Q5... 231 2e-60
sp|Q82WB9|MDH_NITEU Malate dehydrogenase 229 4e-60
>sp|Q04820|MDHC_ECHGR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 281 bits (720), Expect = 9e-76
Identities = 143/226 (63%), Positives = 172/226 (76%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
P+ ++TGAAGQI Y L + VA+GN+FG+ Q I LHLLDIP+ L+G++MEL+DCAF
Sbjct: 4 PLRVLITGAAGQIAYNLSNMVANGNLFGKDQQIILHLLDIPEAKTVLDGVVMELQDCAFT 63
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L + T L+EAF D+D ALMVGAMPRK+GMER+DLL +NV IFK QGEALDKYAKKT
Sbjct: 64 VLAGIVPTHCLKEAFTDIDVALMVGAMPRKQGMERRDLLSSNVKIFKEQGEALDKYAKKT 123
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
V+V VVGNPANTNCLIMSKYAPSIPKENF+ALTRLDHNRAI Q+A KAGVP V+ I
Sbjct: 124 VKVLVVGNPANTNCLIMSKYAPSIPKENFTALTRLDHNRAIYQVAAKAGVPNTCVKNVCI 183
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHSN Q+PD H +V DGK+ + + DE +K F+ VQ
Sbjct: 184 WGNHSNKQFPDLSHAVVT-KDGKQHPAKELINDEKWVKEVFIPCVQ 228
>sp|P11708|MDHC_PIG Malate dehydrogenase, cytoplasmic
Length = 334
Score = 279 bits (714), Expect = 4e-75
Identities = 145/226 (64%), Positives = 175/226 (77%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
PI ++TGAAGQI Y L++S+ +G+VFG+ QPI L LLDI M+ L+G++MEL+DCA P
Sbjct: 4 PIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALP 63
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L V AT + AF D+D A++VG+MPR++GMERKDLLKANV IFK QG ALDKYAKK+
Sbjct: 64 LLKDVIATDKEEIAFKDLDVAILVGSMPRRDGMERKDLLKANVKIFKCQGAALDKYAKKS 123
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
V+V VVGNPANTNCL SK APSIPKENFS LTRLDHNRA AQIA K GV D V+ II
Sbjct: 124 VKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTSDDVKNVII 183
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS+TQYPD +H VK KEV V +AVKD++ LK +F+ TVQ
Sbjct: 184 WGNHSSTQYPDVNHAKVK-LQAKEVGVYEAVKDDSWLKGEFITTVQ 228
>sp|P40925|MDHC_HUMAN Malate dehydrogenase, cytoplasmic
Length = 334
Score = 279 bits (713), Expect = 6e-75
Identities = 145/226 (64%), Positives = 175/226 (77%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
PI ++TGAAGQI Y L++S+ +G+VFG+ QPI L LLDI M+ L+G++MEL+DCA P
Sbjct: 4 PIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALP 63
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L V AT AF D+D A++VG+MPR+EGMERKDLLKANV IFK QG ALDKYAKK+
Sbjct: 64 LLKDVIATDKEDVAFKDLDVAILVGSMPRREGMERKDLLKANVKIFKSQGAALDKYAKKS 123
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
V+V VVGNPANTNCL SK APSIPKENFS LTRLDHNRA AQIA K GV + V+ II
Sbjct: 124 VKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKAQIALKLGVTANDVKNVII 183
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS+TQYPD +H VK GKEV V +A+KD++ LK +F+ TVQ
Sbjct: 184 WGNHSSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLKGEFVTTVQ 228
>sp|P14152|MDHC_MOUSE Malate dehydrogenase, cytoplasmic
Length = 334
Score = 278 bits (712), Expect = 7e-75
Identities = 144/226 (63%), Positives = 176/226 (77%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
PI ++TGAAGQI Y L++S+ +G+VFG+ QPI L LLDI M+ L+G++MEL+DCA P
Sbjct: 4 PIRVLVTGAAGQIAYSLLYSIGNGSVFGKDQPIILVLLDITPMMGVLDGVLMELQDCALP 63
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L V AT + AF D+D A++VG+MPR+EGMERKDLLKANV IFK QG AL+KYAKK+
Sbjct: 64 LLQDVIATDKEEIAFKDLDVAVLVGSMPRREGMERKDLLKANVKIFKSQGTALEKYAKKS 123
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
V+V VVGNPANTNCL SK APSIPKENFS LTRLDHNRA +QIA K GV D V+ II
Sbjct: 124 VKVIVVGNPANTNCLTASKSAPSIPKENFSCLTRLDHNRAKSQIALKLGVTADDVKNVII 183
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS+TQYPD +H VK GKEV V +A+KD++ LK +F+ TVQ
Sbjct: 184 WGNHSSTQYPDVNHAKVK-LQGKEVGVYEALKDDSWLKGEFITTVQ 228
>sp|Q83C87|MDH_COXBU Malate dehydrogenase
Length = 328
Score = 240 bits (612), Expect = 3e-63
Identities = 120/225 (53%), Positives = 158/225 (70%)
Frame = +1
Query: 7 ITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFPQ 186
+ +TGAAGQIGY L+ +ASG FG ++LHLL+I +P+L G++MELEDCAFP
Sbjct: 5 VKVAVTGAAGQIGYALLFRLASGQAFGLDTTVDLHLLEIEPALPALKGVVMELEDCAFPL 64
Query: 187 LISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKTV 366
L ++ T+D + AF+DV++AL+VGA PRK GMERKDLL+ N +IF QG+A+++ A V
Sbjct: 65 LRNMVVTSDPRVAFNDVNWALLVGAAPRKAGMERKDLLEKNGSIFAGQGKAINENAASDV 124
Query: 367 RVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCIIW 546
R+ VVGNP NTNCLI AP IPK+ F A+TRLD NRAI Q+A KAGV V+ IIW
Sbjct: 125 RIFVVGNPCNTNCLIAMNNAPDIPKDRFYAMTRLDQNRAIGQLALKAGVDVPSVKNMIIW 184
Query: 547 GNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
GNHS+TQYPDF+H + G EV ++D+ L NDF+ +Q
Sbjct: 185 GNHSSTQYPDFYHATIDGKPATEV-----IRDKNWLLNDFISVIQ 224
>sp|P57106|MDHD_ARATH Malate dehydrogenase, cytoplasmic 2
Length = 332
Score = 233 bits (595), Expect = 3e-61
Identities = 119/226 (52%), Positives = 155/226 (68%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
P+ ++TGAAGQIGY LV +A G + G QP+ LH+LDIP +LNG+ MEL D AFP
Sbjct: 5 PVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPFAAEALNGVKMELVDAAFP 64
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L V AT D EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A
Sbjct: 65 LLKGVVATTDAVEACTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQASALEKHAAPN 124
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
+V VV NPANTN LI+ ++APSIP++N + LTRLDHNRA+ Q++ + VP V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPEKNITCLTRLDHNRALGQVSERLSVPVSDVKNVII 184
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS+TQYPD +H VK G E V + VK++ L +F+ TVQ
Sbjct: 185 WGNHSSTQYPDVNHATVKTSVG-EKPVRELVKNDEWLNGEFISTVQ 229
>sp|P93819|MDHC1_ARATH Malate dehydrogenase, cytoplasmic 1
Length = 332
Score = 233 bits (594), Expect = 4e-61
Identities = 119/226 (52%), Positives = 154/226 (68%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
P+ ++TGAAGQIGY LV +A G + G QP+ LH+LDIP +LNG+ MEL D AFP
Sbjct: 5 PVRVLVTGAAGQIGYALVPMIARGIMLGADQPVILHMLDIPPAAEALNGVKMELIDAAFP 64
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L V AT D E V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+K+A
Sbjct: 65 LLKGVVATTDAVEGCTGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKSQAAALEKHAAPN 124
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
+V VV NPANTN LI+ ++APSIP++N S LTRLDHNRA+ QI+ + VP V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLSVPVSDVKNVII 184
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS++QYPD +H V+ G E V + VKD+ L +F+ TVQ
Sbjct: 185 WGNHSSSQYPDVNHAKVQTSSG-EKPVRELVKDDAWLDGEFISTVQ 229
>sp|O24047|MDHC_MESCR Malate dehydrogenase, cytoplasmic
Length = 332
Score = 232 bits (592), Expect = 6e-61
Identities = 118/226 (52%), Positives = 154/226 (68%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
P+ ++TGAAGQIGY LV +A G + G +QP+ LH+LDIP +LNG+ MEL D AFP
Sbjct: 5 PLRVLVTGAAGQIGYALVPMIARGIMLGANQPVILHMLDIPPAAEALNGVKMELVDAAFP 64
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L V AT D EA V+ A+MVG PRKEGMERKD++ NV+I+K Q AL+++A
Sbjct: 65 LLKGVVATTDAAEACKGVNVAVMVGGFPRKEGMERKDVMSKNVSIYKAQASALEQHAAPN 124
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
+V VV NPANTN LI+ ++APSIP++N S LTRLDHNRA+ QI+ + V V+ II
Sbjct: 125 CKVLVVANPANTNALILKEFAPSIPEKNISCLTRLDHNRALGQISERLNVQVSDVKNVII 184
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS+TQYPD +H VK G + V + V D+ L +F+ TVQ
Sbjct: 185 WGNHSSTQYPDVNHATVK-TQGVDKPVRELVADDAWLNGEFITTVQ 229
>sp|P10584|MDH_THETH Malate dehydrogenase
sp|Q5SKV7|MDH_THET8 Malate dehydrogenase
sp|P61977|MDH_THET2 Malate dehydrogenase
Length = 327
Score = 231 bits (588), Expect = 2e-60
Identities = 113/205 (55%), Positives = 144/205 (70%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
P+ +TGAAGQIGY L+ +A+G + G+ QP+ L LL+IPQ + +L G++MELEDCAFP
Sbjct: 4 PVRVAVTGAAGQIGYSLLFRIAAGEMLGKDQPVILQLLEIPQAMKALEGVVMELEDCAFP 63
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L ++AT D + AF D D+AL+VGA PRK GMER+DLL+ N IF QG AL + AKK
Sbjct: 64 LLAGLEATDDPKVAFKDADYALLVGAAPRKAGMERRDLLQVNGKIFTEQGRALAEVAKKD 123
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
V+V VVGNPANTN LI K AP + NF+A+TRLDHNRA AQ+A K G D +RR +
Sbjct: 124 VKVLVVGNPANTNALIAYKNAPGLNPRNFTAMTRLDHNRAKAQLAKKTGTGVDRIRRMTV 183
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEV 618
WGNHS+T +PD H V G E+
Sbjct: 184 WGNHSSTMFPDLFHAEVDGRPALEL 208
>sp|Q82WB9|MDH_NITEU Malate dehydrogenase
Length = 327
Score = 229 bits (585), Expect = 4e-60
Identities = 112/226 (49%), Positives = 152/226 (67%)
Frame = +1
Query: 4 PITAVLTGAAGQIGYCLVHSVASGNVFGQHQPINLHLLDIPQMVPSLNGLIMELEDCAFP 183
PI +TGAAGQI Y L+ +A+G++ G QP+ L LLDIP+ L+G++MEL+DCAFP
Sbjct: 4 PIRIAVTGAAGQISYSLLFRIAAGDMLGSSQPVILQLLDIPESGKVLDGVLMELQDCAFP 63
Query: 184 QLISVKATADLQEAFHDVDFALMVGAMPRKEGMERKDLLKANVNIFKVQGEALDKYAKKT 363
L + T D AF D A++VGA PR +GMERKDLL+ N IF+ QG AL++ K+
Sbjct: 64 LLTDIIVTHDPMIAFDQADIAILVGARPRGKGMERKDLLQTNGEIFREQGRALNQVVKRD 123
Query: 364 VRVCVVGNPANTNCLIMSKYAPSIPKENFSALTRLDHNRAIAQIAGKAGVPCDHVRRCII 543
++ VVGNPANTN LI K AP + ENFS + RLDHNRA++Q+A K P H+R+ I+
Sbjct: 124 AKILVVGNPANTNTLITMKNAPDLSPENFSGMLRLDHNRALSQVAMKLNQPVSHIRKMIV 183
Query: 544 WGNHSNTQYPDFHHGLVKGHDGKEVSVLDAVKDET*LKNDFLKTVQ 681
WGNHS+TQ+PD H + V+D +KD+T ++N F+ TVQ
Sbjct: 184 WGNHSSTQFPDLSHAEI-----DHQKVIDLIKDQTWVENSFIPTVQ 224
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,494,880
Number of Sequences: 369166
Number of extensions: 1572914
Number of successful extensions: 4227
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4103
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4201
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5830600200
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)