Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_D02
(328 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q43564|PRP1_MEDTR Repetitive proline-rich cell wall prot... 32 0.34
sp|Q40358|PRP_MEDSA Repetitive proline-rich cell wall prote... 32 0.45
sp|Q40375|PRP2_MEDTR Repetitive proline-rich cell wall prot... 30 1.7
sp|Q9R1D7|GBF1_CRIGR Golgi-specific brefeldin A-resistance ... 30 2.2
sp|Q9M081|SC24B_ARATH Putative protein transport protein Se... 29 3.8
sp|Q04693|RSE1_YEAST Pre-mRNA splicing factor RSE1 (RNA spl... 29 3.8
sp|O83004|GLPD_PSETO Glycerol-3-phosphate dehydrogenase 29 3.8
sp|Q96S59|RANB9_HUMAN Ran-binding protein 9 (RanBP9) (RanBP... 28 4.9
sp|Q8PUV4|WRBA_METMA Flavoprotein wrbA 28 4.9
sp|P27658|CO8A1_HUMAN Collagen alpha 1(VIII) chain precurso... 28 6.5
>sp|Q43564|PRP1_MEDTR Repetitive proline-rich cell wall protein 1 precursor
Length = 206
Score = 32.3 bits (72), Expect = 0.34
Identities = 19/60 (31%), Positives = 22/60 (36%)
Frame = -3
Query: 257 PQGIHAYSGPKTCNPVSHKTXXXXXXXXLPPYGKNPVLCPEATLPIVTSFPVLALPINNP 78
PQG+ Y P P +K PP K PV P P V PV P+ P
Sbjct: 18 PQGLANYEKPPVYQPPVYKPPVEKPPVYKPPVEKPPVYKPPVEKPPVYKPPVYKPPVYKP 77
>sp|Q40358|PRP_MEDSA Repetitive proline-rich cell wall protein precursor (MSPRP)
Length = 236
Score = 32.0 bits (71), Expect = 0.45
Identities = 19/60 (31%), Positives = 22/60 (36%)
Frame = -3
Query: 257 PQGIHAYSGPKTCNPVSHKTXXXXXXXXLPPYGKNPVLCPEATLPIVTSFPVLALPINNP 78
PQG+ Y P P +K PP K PV P P V PV P+ P
Sbjct: 18 PQGLANYDKPPVYQPPVYKPPVEKPPVYKPPVEKPPVYKPPVYKPPVEKPPVYKPPVVKP 77
>sp|Q40375|PRP2_MEDTR Repetitive proline-rich cell wall protein 2 precursor
Length = 371
Score = 30.0 bits (66), Expect = 1.7
Identities = 18/60 (30%), Positives = 22/60 (36%)
Frame = -3
Query: 257 PQGIHAYSGPKTCNPVSHKTXXXXXXXXLPPYGKNPVLCPEATLPIVTSFPVLALPINNP 78
P+G+ Y P P +K PP K PV P P V PV P+ P
Sbjct: 18 PRGLANYDKPPVYQPPVYKPPVEKPPVYKPPVEKPPVYKPPVEKPPVYKPPVEKPPVYKP 77
>sp|Q9R1D7|GBF1_CRIGR Golgi-specific brefeldin A-resistance guanine nucleotide exchange
factor 1 (BFA-resistant GEF 1)
Length = 1856
Score = 29.6 bits (65), Expect = 2.2
Identities = 20/65 (30%), Positives = 28/65 (43%), Gaps = 3/65 (4%)
Frame = -3
Query: 272 GACPF--PQGIHAYSGPKTCNPV-SHKTXXXXXXXXLPPYGKNPVLCPEATLPIVTSFPV 102
GAC P+G A S +PV S + PP + P++ T P+ P
Sbjct: 1761 GACDSEKPEGTRATSSSSPGSPVASSPSRLSPSPEGPPPLAQPPLILQPLTSPLQVGVPP 1820
Query: 101 LALPI 87
+ALPI
Sbjct: 1821 MALPI 1825
>sp|Q9M081|SC24B_ARATH Putative protein transport protein Sec24-like At4g32640
Length = 1069
Score = 28.9 bits (63), Expect = 3.8
Identities = 22/85 (25%), Positives = 32/85 (37%)
Frame = -3
Query: 293 PPTTAFNGACPFPQGIHAYSGPKTCNPVSHKTXXXXXXXXLPPYGKNPVLCPEATLPIVT 114
PP+ + P P G H+ SG P + + P+G+ V + LP
Sbjct: 226 PPSAPYARTPPQPLGSHSLSGNPPLTPFTAPSMPPPATFPGAPHGRPAV----SGLPYGP 281
Query: 113 SFPVLALPINNPGAAGIALYGT*PL 39
+A P+ PG YG PL
Sbjct: 282 PSAQVAPPLGFPGQMQPPRYGMGPL 306
>sp|Q04693|RSE1_YEAST Pre-mRNA splicing factor RSE1 (RNA splicing and ER to Golgi
transport factor 1) (Spliceosome associated protein 130)
Length = 1361
Score = 28.9 bits (63), Expect = 3.8
Identities = 12/24 (50%), Positives = 16/24 (66%), Gaps = 1/24 (4%)
Frame = +3
Query: 30 SRSKWSSTV*CYSCCTWIIY-WKS 98
S +KW S V C+S TW+ Y WK+
Sbjct: 811 SGAKWMSCVVCHSSSTWVSYTWKN 834
>sp|O83004|GLPD_PSETO Glycerol-3-phosphate dehydrogenase
Length = 513
Score = 28.9 bits (63), Expect = 3.8
Identities = 12/21 (57%), Positives = 16/21 (76%)
Frame = +1
Query: 256 GNGQAPLNAVVGGALTTMSRL 318
GNG+AP+ +V GG LTT +L
Sbjct: 351 GNGEAPILSVFGGKLTTYRKL 371
>sp|Q96S59|RANB9_HUMAN Ran-binding protein 9 (RanBP9) (RanBP7) (Ran-binding protein M)
(RanBPM) (BPM90) (BPM-L)
Length = 729
Score = 28.5 bits (62), Expect = 4.9
Identities = 16/46 (34%), Positives = 18/46 (39%)
Frame = +1
Query: 31 PGVSGQVPYNAIPAAPGLFIGRAKTGNDVTIGKVASGHNTGFFPYG 168
P S P + PA PGL G G T VA FP+G
Sbjct: 91 PPASAAAPASGPPAPPGLAAGPGPAGGAPTPALVAGSSAAAPFPHG 136
>sp|Q8PUV4|WRBA_METMA Flavoprotein wrbA
Length = 209
Score = 28.5 bits (62), Expect = 4.9
Identities = 19/63 (30%), Positives = 30/63 (47%), Gaps = 1/63 (1%)
Frame = +1
Query: 112 DVTIGKVASGHNTGFFPYGGREDNISNYEV-LCDTGLHVFGPEYAWIPCGNGQAPLNAVV 288
D +GKV S + +GG+E I ++ V L G+ + G YA Q +N +
Sbjct: 110 DAFVGKVGSVFTSSGTQHGGQESTILSFHVTLLHLGMVIVGLPYA----EKRQTIMNEIT 165
Query: 289 GGA 297
GG+
Sbjct: 166 GGS 168
>sp|P27658|CO8A1_HUMAN Collagen alpha 1(VIII) chain precursor (Endothelial collagen)
Length = 744
Score = 28.1 bits (61), Expect = 6.5
Identities = 21/66 (31%), Positives = 23/66 (34%)
Frame = -3
Query: 263 PFPQGIHAYSGPKTCNPVSHKTXXXXXXXXLPPYGKNPVLCPEATLPIVTSFPVLALPIN 84
P PQG+ G K PP PV P P VT FP P+
Sbjct: 231 PGPQGLRGPKGDKGFGMPGAPGVKGPPGMHGPP---GPVGLPGVGKPGVTGFPGPQGPLG 287
Query: 83 NPGAAG 66
PGA G
Sbjct: 288 KPGAPG 293
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,729,131
Number of Sequences: 369166
Number of extensions: 805526
Number of successful extensions: 2121
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1992
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2119
length of database: 68,354,980
effective HSP length: 77
effective length of database: 54,130,385
effective search space used: 1678041935
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)