Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_C20
(513 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9XSY9|OSTP_SHEEP Osteopontin precursor (Bone sialoprote... 31 2.0
sp|Q6P902|TXND2_MOUSE Thioredoxin domain-containing protein... 30 2.6
sp|P40021|YEM3_YEAST Hypothetical 119.3 kDa protein in PIP1... 30 3.3
sp|Q9AJ82|SCA4_RICMN Antigenic heat-stable 120 kDa protein ... 30 3.3
sp|Q9AJ79|SCA4_RICJA Antigenic heat-stable 120 kDa protein ... 30 4.4
sp|P57082|TBX4_HUMAN T-box transcription factor TBX4 (T-box... 29 5.7
sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2) 29 5.7
sp|Q52658|SCA4_RICCN Antigenic heat-stable 120 kDa protein ... 29 7.5
sp|Q9Z762|ENGA_CHLPN GTP-binding protein engA 28 9.7
sp|Q8YEB3|IF2_BRUME Translation initiation factor IF-2 28 9.7
>sp|Q9XSY9|OSTP_SHEEP Osteopontin precursor (Bone sialoprotein-1) (Secreted
phosphoprotein 1) (SPP-1)
Length = 278
Score = 30.8 bits (68), Expect = 2.0
Identities = 29/116 (25%), Positives = 46/116 (39%), Gaps = 6/116 (5%)
Frame = +3
Query: 72 LKRPSKPIEKVNYHTEQKPNGNIMSY-EERSIRYAEERRRILGSQSEMIEEDII--EVLN 242
LK SK + N + + S+ E + A ++ L SE D + E+
Sbjct: 160 LKSKSKKFRRSNVESPDATEEDFTSHIESEEMHDAPKKTSQLTDHSEETNSDELPKELTP 219
Query: 243 KFNE---YSSRSEGARETKLpreSHKKYNSMGSDSLNNGDRRENPMILHRLSKADS 401
K E +S+R E +KL +E H + + D + D+R I H L S
Sbjct: 220 KAKEESKHSNRIESQENSKLSQEFHSLEDKLDLDHKSEEDKRLKIRISHELDSVSS 275
>sp|Q6P902|TXND2_MOUSE Thioredoxin domain-containing protein 2 (Spermatid-specific
thioredoxin-1) (Sptrx-1) (Thioredoxin-4)
Length = 515
Score = 30.4 bits (67), Expect = 2.6
Identities = 15/57 (26%), Positives = 28/57 (49%)
Frame = +3
Query: 114 TEQKPNGNIMSYEERSIRYAEERRRILGSQSEMIEEDIIEVLNKFNEYSSRSEGARE 284
T Q GN + E ++ +E + LG++ E +EE ++ V+ E+ + A E
Sbjct: 374 TIQSQEGNTIKSSEEDVQLSESKLLGLGAEIETLEEGLVRVIKDKEEFEEVLKDAGE 430
>sp|P40021|YEM3_YEAST Hypothetical 119.3 kDa protein in PIP1-GLN3 intergenic region
Length = 1076
Score = 30.0 bits (66), Expect = 3.3
Identities = 27/99 (27%), Positives = 40/99 (40%), Gaps = 11/99 (11%)
Frame = +3
Query: 102 VNYHTEQKPNGNIMSYEERSIRYAEERRRILGSQSEMIEEDIIEVLNKFNEYSSRSEGAR 281
V Y+++ +P N+ + Y +E IE+D E N+ NE S
Sbjct: 466 VKYYSKPEPAANVYIDD----LYEDEN---FDDDMNCIEDD--ESGNEGNEICGLSTRFE 516
Query: 282 ETKLpreSHKKYNSM-----------GSDSLNNGDRREN 365
ET L KK+N + G D+ NNGD E+
Sbjct: 517 ETSLKSNKVKKFNDLFNLSDDDEEEDGKDNSNNGDENES 555
>sp|Q9AJ82|SCA4_RICMN Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
(Protein PS 120)
Length = 1011
Score = 30.0 bits (66), Expect = 3.3
Identities = 31/109 (28%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
Frame = +3
Query: 54 QPKFQILKRPSK-PIEKVN-YHTEQKPNGNIMSYEER---SIRYAEERRRILGSQSEMIE 218
Q IL SK +EKVN + PNGN+ + EE+ SI+ +E + G++S E
Sbjct: 841 QETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEESIKKVDELVKAFGTKSSTEE 900
Query: 219 EDIIEVLNKFNEYSSRSEGARET--KLpreSHKKYNSMGSDSLNNGDRR 359
+ N ++ + E +T KL +E K+ ++ + S+ D R
Sbjct: 901 QQSFIKTNLIDDKTLSKEVRLQTIDKLLQE-QKRAEAIENPSVKTEDVR 948
>sp|Q9AJ79|SCA4_RICJA Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
(Protein PS 120) (rps120)
Length = 1018
Score = 29.6 bits (65), Expect = 4.4
Identities = 31/109 (28%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
Frame = +3
Query: 54 QPKFQILKRPSK-PIEKVN-YHTEQKPNGNIMSYEER---SIRYAEERRRILGSQSEMIE 218
Q IL SK +EKVN + PNGN+ + EE+ SI+ +E + G++S E
Sbjct: 842 QETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEESIKKVDELVKAFGTKSSTEE 901
Query: 219 EDIIEVLNKFNEYSSRSEGARET--KLpreSHKKYNSMGSDSLNNGDRR 359
+ N ++ + E +T KL +E K+ ++ + S+ D R
Sbjct: 902 QQSFIKANLIDDKTLSKEIRLQTIDKLLQE-QKRAEAIENPSVKTEDVR 949
>sp|P57082|TBX4_HUMAN T-box transcription factor TBX4 (T-box protein 4)
Length = 545
Score = 29.3 bits (64), Expect = 5.7
Identities = 15/58 (25%), Positives = 28/58 (48%)
Frame = -1
Query: 486 IKPLLNXXXXXXYHQSRNTLRHQQITQPTNQPCSICAESWDSRVYHHYLENRSPSNYI 313
+ PLL ++Q N H Q+ +P + P S DS +++H L+ R + ++
Sbjct: 290 VGPLLGTHQALQHYQHENGA-HSQLAEPQDLPLSTFPTQRDSSLFYHCLKRRDGTRHL 346
>sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2)
Length = 1679
Score = 29.3 bits (64), Expect = 5.7
Identities = 34/158 (21%), Positives = 69/158 (43%), Gaps = 25/158 (15%)
Frame = +3
Query: 3 EDFILYFLMEFCLKTMSQPKFQILKRPSKPIEKVNYHTEQKPNGNIMSYEERSIRYAE-- 176
ED I FL +++ + +L++ K I K E+ N++ E +S Y+
Sbjct: 2 EDKISEFL-NVPFESLQGVTYPVLRKLYKKIAKFERSEEEVTKLNVLVDEIKSQYYSRIS 60
Query: 177 ERRRILGSQSEM---IEEDIIEVLNKFNEYSSR---------------SEGARETKLpre 302
+ +++L SE +E++ + ++ NE SR E RE +
Sbjct: 61 KLKQLLDESSEQKNTAKEELNGLKDQLNEERSRYRREIDALKKQLHVSHEAMREVNDEKR 120
Query: 303 SHKKYN-----SMGSDSLNNGDRRENPMILHRLSKADS 401
++Y+ G+DSLN+ +EN ++ +L + ++
Sbjct: 121 VKEEYDIWQSRDQGNDSLNDDLNKENKLLRRKLMEMEN 158
>sp|Q52658|SCA4_RICCN Antigenic heat-stable 120 kDa protein (PS120) (120 kDa antigen)
(Protein PS 120) (RCA)
Length = 1022
Score = 28.9 bits (63), Expect = 7.5
Identities = 30/109 (27%), Positives = 52/109 (47%), Gaps = 7/109 (6%)
Frame = +3
Query: 54 QPKFQILKRPSK-PIEKVN-YHTEQKPNGNIMSYEER---SIRYAEERRRILGSQSEMIE 218
Q IL SK +EKVN + PNGN+ + EE+ +I+ +E + G++S E
Sbjct: 846 QETSSILNNISKLAVEKVNNFRAMLSPNGNLKTLEEKKEEAIKKVDELVKAFGTKSSTEE 905
Query: 219 EDIIEVLNKFNEYSSRSEGARET--KLpreSHKKYNSMGSDSLNNGDRR 359
+ N ++ + E +T KL +E K+ ++ + S+ D R
Sbjct: 906 QQSFIKTNLIDDKTLSKEVRLQTIDKLLQE-QKRSEAIENPSVKTEDVR 953
>sp|Q9Z762|ENGA_CHLPN GTP-binding protein engA
Length = 487
Score = 28.5 bits (62), Expect = 9.7
Identities = 15/41 (36%), Positives = 23/41 (56%)
Frame = -1
Query: 420 QQITQPTNQPCSICAESWDSRVYHHYLENRSPSNYIFYGIP 298
Q+ T P I A+S ++ Y +YL+N S++ YGIP
Sbjct: 434 QKTTTPLQFLLFINAKSLLTKHYEYYLKNTLKSSFNLYGIP 474
>sp|Q8YEB3|IF2_BRUME Translation initiation factor IF-2
Length = 959
Score = 28.5 bits (62), Expect = 9.7
Identities = 24/91 (26%), Positives = 41/91 (45%), Gaps = 4/91 (4%)
Frame = +3
Query: 54 QPKFQILKRPSKPIEK----VNYHTEQKPNGNIMSYEERSIRYAEERRRILGSQSEMIEE 221
+P Q +RP +P ++ +N + + + + EE IR EER R + EE
Sbjct: 129 RPGGQQAQRP-RPADRSGMVLNTLSRSEMDARRRALEEAQIREVEERARAVEEAKRRAEE 187
Query: 222 DIIEVLNKFNEYSSRSEGARETKLpreSHKK 314
D K +E S+R + E +L E+ +
Sbjct: 188 DARRA--KEHEESARRQAEEEARLKAEAEAR 216
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 53,362,047
Number of Sequences: 369166
Number of extensions: 991570
Number of successful extensions: 3106
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3019
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3104
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3304927425
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)