Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_A23
(526 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P41891|GAR2_SCHPO Protein gar2 36 0.049
sp|P48415|SEC16_YEAST Multidomain vesicle coat protein 35 0.11
sp|Q8K2H1|PPHLN_MOUSE Periphilin 1 34 0.19
sp|Q6CMM2|MAD1_KLULA Spindle assembly checkpoint component ... 34 0.25
sp|Q9QZM6|NCKX1_RAT Sodium/potassium/calcium exchanger 1 (N... 32 1.2
sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor 32 1.2
sp|P53847|YNZ8_YEAST Hypothetical 88.1 kDa protein in ATX1-... 31 1.6
sp|O36021|YEK9_SCHPO Hypothetical protein C4F10.09c in chro... 31 2.1
sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2) 31 2.1
sp|O67124|RAD50_AQUAE Probable DNA double-strand break repa... 31 2.1
>sp|P41891|GAR2_SCHPO Protein gar2
Length = 500
Score = 36.2 bits (82), Expect = 0.049
Identities = 32/108 (29%), Positives = 53/108 (49%), Gaps = 3/108 (2%)
Frame = +2
Query: 8 KTERKRESRWESSKISKEIKVADGQSKDNEKVE---DQTTNLFSDQSPYCEAAMKNESEI 178
KTE K+ES ESS S+ + + K EK E D ++ S +S ++ ++E E
Sbjct: 129 KTEEKKESSSESSSSSESEEEEEAVVKIEEKKESSSDSSSESSSSESESESSSSESEEEE 188
Query: 179 QEAQKITDEHKLSIAEHSSDLMIETLEVNIESNFEETIENLDKSDNAD 322
+ +K T+E K +E SSD ES+ + + E+ D ++D
Sbjct: 189 EVVEK-TEEKKEGSSESSSD---------SESSSDSSSESGDSDSSSD 226
>sp|P48415|SEC16_YEAST Multidomain vesicle coat protein
Length = 2195
Score = 35.0 bits (79), Expect = 0.11
Identities = 29/100 (29%), Positives = 44/100 (44%), Gaps = 6/100 (6%)
Frame = +2
Query: 35 WESSKISKEIKVADGQSKDNEKVEDQTTNLFSDQSPYCE------AAMKNESEIQEAQKI 196
WES+ K AD SK EK ED +D+ E ++ K E+ +Q + +
Sbjct: 448 WESTD-----KNADVTSKSQEKHEDLFAASGNDEKLPWEVSDGEVSSGKTENSMQTSTEK 502
Query: 197 TDEHKLSIAEHSSDLMIETLEVNIESNFEETIENLDKSDN 316
E K S E+ DL+ + S E+T+ N D + N
Sbjct: 503 IAEQKFSFLENDDDLLDDDDSFLASSEEEDTVPNTDNTTN 542
>sp|Q8K2H1|PPHLN_MOUSE Periphilin 1
Length = 381
Score = 34.3 bits (77), Expect = 0.19
Identities = 26/101 (25%), Positives = 43/101 (42%), Gaps = 7/101 (6%)
Frame = +2
Query: 14 ERKRESRWESSKISKEIKVADGQSKDNEKVED-------QTTNLFSDQSPYCEAAMKNES 172
ER R + S+ K +V + NE + Q+ D SP +A+ +
Sbjct: 171 ERSRTHSFHQSQHRKSSRVGASYKRQNEAIRGRGKERSIQSVKTSRDASPSSSSAVASSK 230
Query: 173 EIQEAQKITDEHKLSIAEHSSDLMIETLEVNIESNFEETIE 295
+ + ++T++ +AE S ETLE + ESN E E
Sbjct: 231 ALDKPSRLTEKE---LAEAESKWANETLEKSDESNLAEMNE 268
>sp|Q6CMM2|MAD1_KLULA Spindle assembly checkpoint component MAD1 (Mitotic arrest
deficient protein 1)
Length = 648
Score = 33.9 bits (76), Expect = 0.25
Identities = 22/80 (27%), Positives = 43/80 (53%)
Frame = +2
Query: 38 ESSKISKEIKVADGQSKDNEKVEDQTTNLFSDQSPYCEAAMKNESEIQEAQKITDEHKLS 217
E+ K+ +E+K D K++EK+ T L + ++KNE ++E + + HKLS
Sbjct: 76 ENIKLKEELKSKDALPKEDEKI---ITTLRTQV-----VSLKNEVNLKEQRIVELAHKLS 127
Query: 218 IAEHSSDLMIETLEVNIESN 277
++ IE++++ IE +
Sbjct: 128 SKQNEVTNAIESMKLEIEGS 147
>sp|Q9QZM6|NCKX1_RAT Sodium/potassium/calcium exchanger 1 (Na(+)/K(+)/Ca(2+)-exchange
protein 1) (Retinal rod Na-Ca+K exchanger)
Length = 1181
Score = 31.6 bits (70), Expect = 1.2
Identities = 24/107 (22%), Positives = 45/107 (42%)
Frame = +2
Query: 2 DTKTERKRESRWESSKISKEIKVADGQSKDNEKVEDQTTNLFSDQSPYCEAAMKNESEIQ 181
+T+ ERK + ++ A+G+ D+E + N+ E +++E E +
Sbjct: 844 ETEAERKETNHEGETE-------AEGKEADHEGETEAEGNVEHQGETEAEGKVEHEGETE 896
Query: 182 EAQKITDEHKLSIAEHSSDLMIETLEVNIESNFEETIENLDKSDNAD 322
+K DEH+ + D ++ E E+N E+ E AD
Sbjct: 897 AGEK--DEHEGQSETQADDTEVKDGEGEAEANAEDQCETAQGEKGAD 941
>sp|P13816|GARP_PLAFF Glutamic acid-rich protein precursor
Length = 678
Score = 31.6 bits (70), Expect = 1.2
Identities = 25/105 (23%), Positives = 48/105 (45%)
Frame = +2
Query: 8 KTERKRESRWESSKISKEIKVADGQSKDNEKVEDQTTNLFSDQSPYCEAAMKNESEIQEA 187
K E K+E E + SKE++ + + +++E+ E++ ++ E + E E +E
Sbjct: 547 KEEDKKEESKEVQEESKEVQEDEEEVEEDEEEEEEEEEEEEEEE---EEEEEEEEEEEEE 603
Query: 188 QKITDEHKLSIAEHSSDLMIETLEVNIESNFEETIENLDKSDNAD 322
++ DE AE D E + E + EE + D ++ D
Sbjct: 604 EEDEDEEDEDDAEEDEDDAEEDEDDAEEDDDEEDDDEEDDDEDED 648
>sp|P53847|YNZ8_YEAST Hypothetical 88.1 kDa protein in ATX1-SIP3 intergenic region
Length = 754
Score = 31.2 bits (69), Expect = 1.6
Identities = 16/76 (21%), Positives = 38/76 (50%)
Frame = +2
Query: 80 QSKDNEKVEDQTTNLFSDQSPYCEAAMKNESEIQEAQKITDEHKLSIAEHSSDLMIETLE 259
Q+ + ++ TN S++ ++ ++NE E+ A D+ + + +D + ++
Sbjct: 371 QNLEVVELTTSNTNTMSEKIGKNDSDVQNEKELHNAVSKDDDWNWEVEDDDADAWGDEID 430
Query: 260 VNIESNFEETIENLDK 307
VNI+ E+T + +K
Sbjct: 431 VNIDDEEEKTNQEKEK 446
>sp|O36021|YEK9_SCHPO Hypothetical protein C4F10.09c in chromosome I
Length = 860
Score = 30.8 bits (68), Expect = 2.1
Identities = 26/120 (21%), Positives = 49/120 (40%), Gaps = 17/120 (14%)
Frame = +2
Query: 5 TKTERKRESRWESSKISKEIKVADGQSK--------DNEKVEDQTTNLFSDQSPYCEAAM 160
TK +R E + ++ K + + Q + D+E+++ T++ SD +
Sbjct: 711 TKVDRDEEGEIDEDEVWKALVDSKPQLEMDEEESDFDSEEMDKAMTDMGSDSEQSADEND 770
Query: 161 KNESEIQEAQKITDEHKLSIAEHSSDLMIETLEVNIESN---------FEETIENLDKSD 313
+E +DE LS HS D +T++ + N EE I+ +D S+
Sbjct: 771 NESMASEEKPMFSDEENLSEIAHSEDEFDDTVDFFEDENDLLPFNETDDEEEIQTVDHSE 830
>sp|P40457|MLP2_YEAST Protein MLP2 (Myosin-like protein 2)
Length = 1679
Score = 30.8 bits (68), Expect = 2.1
Identities = 22/82 (26%), Positives = 38/82 (46%), Gaps = 1/82 (1%)
Frame = +2
Query: 56 KEIKVADGQSKDNEKVEDQTTNLFSDQSPYCEAAM-KNESEIQEAQKITDEHKLSIAEHS 232
KE+ K+N+ + + L D + C+A + K +S + AQ + D+H+ E
Sbjct: 999 KEMSQYQSTMKENKDLSELVIRLEKDAAD-CQAELTKTKSSLYSAQDLLDKHERKWMEEK 1057
Query: 233 SDLMIETLEVNIESNFEETIEN 298
+D E + NIE +EN
Sbjct: 1058 ADYERELIS-NIEQTESLRVEN 1078
>sp|O67124|RAD50_AQUAE Probable DNA double-strand break repair rad50 ATPase
Length = 978
Score = 30.8 bits (68), Expect = 2.1
Identities = 27/106 (25%), Positives = 47/106 (44%), Gaps = 1/106 (0%)
Frame = +2
Query: 2 DTKTERKRESRWESSKISKEIKVADGQSKDNEKVEDQTTNLFSDQSPYCEAAMKNESEIQ 181
+ + E + + E +I KE+ K+ E+ +Q F D S E K +E +
Sbjct: 335 EKELEHRLKKLQEIKEILKELSQLSSSLKEKEREYEQAKQEFEDLSERVEKGKKLVAETE 394
Query: 182 E-AQKITDEHKLSIAEHSSDLMIETLEVNIESNFEETIENLDKSDN 316
E +KI + S E++S M E L V ++ +E E + +N
Sbjct: 395 EKLEKI--KELFSEEEYTSLKMKERLLVELQRKLKELKEKEGQLEN 438
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 44,265,369
Number of Sequences: 369166
Number of extensions: 698797
Number of successful extensions: 2367
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2293
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2356
length of database: 68,354,980
effective HSP length: 103
effective length of database: 49,327,275
effective search space used: 3502236525
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)