Planarian EST Database


Dr_sW_028_A18

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_028_A18
         (468 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|P47830|RL27A_XENLA  60S ribosomal protein L27a (L22)           180   2e-45
sp|P18445|RL27A_RAT  60S ribosomal protein L27a                   172   4e-43
sp|Q5REY2|RL27A_PONPY  60S ribosomal protein L27a >gi|117301...   171   6e-43
sp|Q5R1X0|RL27A_PANTR  60S ribosomal protein L27a                 171   6e-43
sp|P14115|RL27A_MOUSE  60S ribosomal protein L27a (L29)           169   2e-42
sp|O01358|RL27A_OSCBR  60S ribosomal protein L27a (Ribosomal...   156   2e-38
sp|P41092|RL27A_DROME  60S ribosomal protein L27a                 154   7e-38
sp|Q27021|RL27A_TENMO  60S ribosomal protein L27a                 143   2e-34
sp|P48160|RL27A_DICDI  60S ribosomal protein L27a                 142   5e-34
sp|Q9BI14|R27A3_ENTHI  60S ribosomal protein L27a-3               141   8e-34
>sp|P47830|RL27A_XENLA 60S ribosomal protein L27a (L22)
          Length = 148

 Score =  180 bits (456), Expect = 2e-45
 Identities = 85/137 (62%), Positives = 101/137 (73%)
 Frame = +2

Query: 14  KAKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHL 193
           K +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMRHYHL
Sbjct: 4   KLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHL 63

Query: 194 RKNRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVI 373
           +KN++FCP+IN+DK+W+LVSE+T   +  N EG AP+ID + AGY+KVLG G LPK PVI
Sbjct: 64  KKNQSFCPTINLDKLWTLVSEQTRLNHAKNLEGPAPIIDAVHAGYYKVLGKGKLPKQPVI 123

Query: 374 VKAKFFSRQAEEKIKSV 424
           VKAKFFSR+AEEKIKSV
Sbjct: 124 VKAKFFSRKAEEKIKSV 140
>sp|P18445|RL27A_RAT 60S ribosomal protein L27a
          Length = 148

 Score =  172 bits (435), Expect = 4e-43
 Identities = 79/137 (57%), Positives = 99/137 (72%)
 Frame = +2

Query: 14  KAKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHL 193
           + +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMRHYHL
Sbjct: 4   RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHL 63

Query: 194 RKNRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVI 373
           ++N++FCP++N+DK+W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVI
Sbjct: 64  KRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKNGVAPIIDVVRSGYYKVLGKGKLPKQPVI 123

Query: 374 VKAKFFSRQAEEKIKSV 424
           VKAKFFSR+AEEKIK V
Sbjct: 124 VKAKFFSRRAEEKIKGV 140
>sp|Q5REY2|RL27A_PONPY 60S ribosomal protein L27a
 sp|P46776|RL27A_HUMAN 60S ribosomal protein L27a
          Length = 148

 Score =  171 bits (434), Expect = 6e-43
 Identities = 79/137 (57%), Positives = 100/137 (72%)
 Frame = +2

Query: 14  KAKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHL 193
           + +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGM+HYHL
Sbjct: 4   RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHL 63

Query: 194 RKNRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVI 373
           ++N++FCP++N+DK+W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVI
Sbjct: 64  KRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVI 123

Query: 374 VKAKFFSRQAEEKIKSV 424
           VKAKFFSR+AEEKIKSV
Sbjct: 124 VKAKFFSRRAEEKIKSV 140
>sp|Q5R1X0|RL27A_PANTR 60S ribosomal protein L27a
          Length = 148

 Score =  171 bits (434), Expect = 6e-43
 Identities = 79/137 (57%), Positives = 100/137 (72%)
 Frame = +2

Query: 14  KAKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHL 193
           + +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGM+HYHL
Sbjct: 4   RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGLHHHRINFDKYHPGYFGKVGMKHYHL 63

Query: 194 RKNRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVI 373
           ++N++FCP++N+DK+W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVI
Sbjct: 64  KRNQSFCPTVNLDKLWTLVSEQTRVNAAKNKTGAAPIIDVVRSGYYKVLGKGKLPKQPVI 123

Query: 374 VKAKFFSRQAEEKIKSV 424
           VKAKFFSR+AEEKIKSV
Sbjct: 124 VKAKFFSRRAEEKIKSV 140
>sp|P14115|RL27A_MOUSE 60S ribosomal protein L27a (L29)
          Length = 148

 Score =  169 bits (429), Expect = 2e-42
 Identities = 79/137 (57%), Positives = 98/137 (71%)
 Frame = +2

Query: 14  KAKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHL 193
           + +KTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMRHYHL
Sbjct: 4   RLRKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMRHYHL 63

Query: 194 RKNRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVI 373
           ++N++FCP++N+DK W+LVSE+T      N  G AP+ID +++GY+KVLG G LPK PVI
Sbjct: 64  KRNQSFCPTVNLDKPWTLVSEQTRVNAAKNKTGVAPIIDVVRSGYYKVLGKGKLPKQPVI 123

Query: 374 VKAKFFSRQAEEKIKSV 424
           VKAKFFSR+AEEKIK V
Sbjct: 124 VKAKFFSRRAEEKIKGV 140
>sp|O01358|RL27A_OSCBR 60S ribosomal protein L27a (Ribosomal protein RPL-27)
          Length = 145

 Score =  156 bits (395), Expect = 2e-38
 Identities = 77/134 (57%), Positives = 96/134 (71%)
 Frame = +2

Query: 20  KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 199
           +KTRKL                      NAGGQHHHRI+ DK+HPGYFGKVGMR +HL K
Sbjct: 6   RKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGQHHHRINRDKYHPGYFGKVGMRVFHLNK 65

Query: 200 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 379
           N  +CP++N+D++W+LV E+  +  K +AE KAPVIDC+KAGYFKVLG G+LPK P+IVK
Sbjct: 66  NHHYCPTVNVDRLWALVPEE--QKTKVSAE-KAPVIDCVKAGYFKVLGKGLLPKQPLIVK 122

Query: 380 AKFFSRQAEEKIKS 421
           AKFFS +AE KIK+
Sbjct: 123 AKFFSHEAENKIKA 136
>sp|P41092|RL27A_DROME 60S ribosomal protein L27a
          Length = 149

 Score =  154 bits (390), Expect = 7e-38
 Identities = 75/139 (53%), Positives = 92/139 (66%)
 Frame = +2

Query: 2   MSKIKAKKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMR 181
           MS IK KKTRKL                      NAGG HHHRI+FDK+HPGYFGKVGMR
Sbjct: 1   MSNIKRKKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKVGMR 60

Query: 182 HYHLRKNRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPK 361
           ++HLR+   F P IN+DK+WSLV  + +   +     KAPVID +K GY+K+LG G LP 
Sbjct: 61  NFHLRRQHKFRPEINLDKLWSLVGAEKFAELEKEKSTKAPVIDLVKFGYYKLLGRGHLPA 120

Query: 362 VPVIVKAKFFSRQAEEKIK 418
            PVIVKAK+FS++AE+KIK
Sbjct: 121 RPVIVKAKYFSKKAEDKIK 139
>sp|Q27021|RL27A_TENMO 60S ribosomal protein L27a
          Length = 148

 Score =  143 bits (361), Expect = 2e-34
 Identities = 68/135 (50%), Positives = 90/135 (66%)
 Frame = +2

Query: 20  KKTRKLXXXXXXXXXXXXXXXXXXXXXXNAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRK 199
           KKTRKL                      NAGG HHHRI+FDK+HPGYFGK+GMR+YHLR+
Sbjct: 6   KKTRKLRGHVSHGHGRIGKHRKHPGGRGNAGGMHHHRINFDKYHPGYFGKLGMRNYHLRR 65

Query: 200 NRTFCPSINIDKIWSLVSEKTYETYKDNAEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVK 379
           N  + P+IN+DK+W+LVSE+T   Y+++ EGKAPVID +KAGY+K+LG G       ++K
Sbjct: 66  NSKWAPAINLDKLWTLVSEQTRSKYQNHPEGKAPVIDIVKAGYYKLLGKGACLTNLSLLK 125

Query: 380 AKFFSRQAEEKIKSV 424
             F   + E+KIK+V
Sbjct: 126 PSFSLNRLEDKIKAV 140
>sp|P48160|RL27A_DICDI 60S ribosomal protein L27a
          Length = 148

 Score =  142 bits (357), Expect = 5e-34
 Identities = 60/107 (56%), Positives = 86/107 (80%)
 Frame = +2

Query: 104 NAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSINIDKIWSLVSEKTYETYKDN 283
           NAGG  HHRI+FDK+HPGYFGK+GMRH+HL +N+  CP+++++KIW+LV E   ++    
Sbjct: 34  NAGGLTHHRINFDKYHPGYFGKLGMRHFHLLRNQYHCPTVSLEKIWTLVPESVRKSLAAK 93

Query: 284 AEGKAPVIDCLKAGYFKVLGNGVLPKVPVIVKAKFFSRQAEEKIKSV 424
            +G APV+D  + G+FKVLG+G+LP  P+IVKA++FS+ AE+KIK+V
Sbjct: 94  NDGTAPVVDVTQKGFFKVLGHGILPTQPIIVKARYFSKVAEKKIKAV 140
>sp|Q9BI14|R27A3_ENTHI 60S ribosomal protein L27a-3
          Length = 149

 Score =  141 bits (355), Expect = 8e-34
 Identities = 64/108 (59%), Positives = 82/108 (75%), Gaps = 1/108 (0%)
 Frame = +2

Query: 104 NAGGQHHHRIHFDKFHPGYFGKVGMRHYHLRKNRTFCPSINIDKIWSLVSEKTYETYKDN 283
           NAGGQHH +  F  FHP YFGK GMR +HL+ N+ +CPSIN+D +WSLV +   E YK+ 
Sbjct: 34  NAGGQHHRKTWFTTFHPDYFGKHGMRVFHLKANKYYCPSINVDSLWSLVGKDVQEQYKNA 93

Query: 284 AEG-KAPVIDCLKAGYFKVLGNGVLPKVPVIVKAKFFSRQAEEKIKSV 424
             G + PVIDC+K GYFKVLG G LPK PVIV+A++FS +A++KIK+V
Sbjct: 94  KTGEEVPVIDCVKHGYFKVLGKGFLPKQPVIVRARYFSEKAQQKIKAV 141
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 46,891,450
Number of Sequences: 369166
Number of extensions: 824391
Number of successful extensions: 2196
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2100
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2159
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2683624230
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)