Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_028_A04
(490 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor... 123 2e-28
sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precurs... 122 4e-28
sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precurs... 121 8e-28
sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precurs... 116 2e-26
sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precurs... 115 5e-26
sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl este... 107 1e-23
sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester ... 106 3e-23
sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl este... 96 4e-20
sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1) 74 1e-13
sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3) 67 3e-11
>sp|P04634|LIPG_RAT Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Lingual lipase)
Length = 395
Score = 123 bits (309), Expect = 2e-28
Identities = 60/150 (40%), Positives = 92/150 (61%), Gaps = 1/150 (0%)
Frame = +1
Query: 43 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 222
+FG + ++ FL + +C +E L CS +F+ G D KNLN SR VY+ H PA
Sbjct: 224 MFGKKMFLPHTYFDDFLGTEVCSREVLDLLCSNTLFIFCGFDKKNLNVSRFDVYLGHNPA 283
Query: 223 GTSVMNIVHYAQSVDNGNFQMYDFGIEG-NIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 399
GTSV + +H+AQ V +G FQ +++G N+ Y PP++DV + +P A+++GGND
Sbjct: 284 GTSVQDFLHWAQLVRSGKFQAFNWGSPSQNMLHYNQKTPPEYDVSAMTVPVAVWNGGNDI 343
Query: 400 LADPEDVSRLLGLVGGDVRFQRFIRYYNHM 489
LADP+DV+ LL + ++ F + I YNH+
Sbjct: 344 LADPQDVAMLLPKL-SNLLFHKEILAYNHL 372
>sp|P07098|LIPG_HUMAN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 122 bits (306), Expect = 4e-28
Identities = 61/150 (40%), Positives = 92/150 (61%), Gaps = 1/150 (0%)
Frame = +1
Query: 43 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 222
+FG ++ +FLA+ +C +E L CS +F++ G D KN N SRL VY+SH PA
Sbjct: 225 IFGDKIFYPHNFFDQFLATEVCSREMLNLLCSNALFIICGFDSKNFNTSRLDVYLSHNPA 284
Query: 223 GTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 399
GTSV N+ H+ Q+V +G FQ YD+G N Y PP ++V + +P A+++GG D
Sbjct: 285 GTSVQNMFHWTQAVKSGKFQAYDWGSPVQNRMHYDQSQPPYYNVTAMNVPIAVWNGGKDL 344
Query: 400 LADPEDVSRLLGLVGGDVRFQRFIRYYNHM 489
LADP+DV LL + ++ + + I +YNH+
Sbjct: 345 LADPQDVGLLLPKL-PNLIYHKEIPFYNHL 373
>sp|P80035|LIPG_CANFA Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 398
Score = 121 bits (304), Expect = 8e-28
Identities = 65/167 (38%), Positives = 99/167 (59%), Gaps = 13/167 (7%)
Frame = +1
Query: 28 ETLLN------------LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDI 171
ETLLN +FG+ + +FLA+ +C +E + CS +F++ G D
Sbjct: 208 ETLLNKLMLVPSFLFKLIFGNKIFYPHHFFDQFLATEVCSRETVDLLCSNALFIICGFDT 267
Query: 172 KNLNESRLPVYISHTPAGTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFD 348
NLN SRL VY+SH PAGTSV N++H++Q+V +G FQ +D+G N+ Y PP ++
Sbjct: 268 MNLNMSRLDVYLSHNPAGTSVQNVLHWSQAVKSGKFQAFDWGSPVQNMMHYHQSMPPYYN 327
Query: 349 VKPVGIPTAIFSGGNDTLADPEDVSRLLGLVGGDVRFQRFIRYYNHM 489
+ + +P A+++GGND LADP DV LL + ++ + R I YNH+
Sbjct: 328 LTDMHVPIAVWNGGNDLLADPHDVDLLLSKL-PNLIYHRKIPPYNHL 373
>sp|Q9CPP7|LIPG_MOUSE Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
Length = 395
Score = 116 bits (291), Expect = 2e-26
Identities = 57/150 (38%), Positives = 91/150 (60%), Gaps = 1/150 (0%)
Frame = +1
Query: 43 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 222
+FG+ M ++ + +FL + +C +E L CS +F+ G D KNLN SR VY+ H PA
Sbjct: 224 IFGNKMFMPHNYLDQFLGTEVCSRELLDLLCSNALFIFCGFDKKNLNVSRFDVYLGHNPA 283
Query: 223 GTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 399
GTS ++ H+AQ +G Q Y++G N+ Y PP +DV + +P A+++GG+D
Sbjct: 284 GTSTQDLFHWAQLAKSGKLQAYNWGSPLQNMLHYNQKTPPYYDVSAMTVPIAVWNGGHDI 343
Query: 400 LADPEDVSRLLGLVGGDVRFQRFIRYYNHM 489
LADP+DV+ LL + ++ + + I YNH+
Sbjct: 344 LADPQDVAMLLPKL-PNLLYHKEILPYNHL 372
>sp|Q29458|LIPG_BOVIN Gastric triacylglycerol lipase precursor (Gastric lipase) (GL)
(Pregastric esterase) (PGE)
Length = 397
Score = 115 bits (288), Expect = 5e-26
Identities = 58/150 (38%), Positives = 89/150 (59%), Gaps = 1/150 (0%)
Frame = +1
Query: 43 LFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPA 222
+FG ++ + +FL +C +E L C +F + G D KN N SRL VYI+H PA
Sbjct: 224 IFGDKMFYPHTFLEQFLGVEMCSRETLDVLCKNALFAITGVDNKNFNMSRLDVYIAHNPA 283
Query: 223 GTSVMNIVHYAQSVDNGNFQMYDFGIE-GNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDT 399
GTSV N +H+ Q+V +G FQ +D+G N+ Y P PP +++ + +P A++S ND
Sbjct: 284 GTSVQNTLHWRQAVKSGKFQAFDWGAPYQNLMHYHQPTPPIYNLTAMNVPIAVWSADNDL 343
Query: 400 LADPEDVSRLLGLVGGDVRFQRFIRYYNHM 489
LADP+DV LL + ++ + + I YNH+
Sbjct: 344 LADPQDVDFLLSKL-SNLIYHKEIPNYNHL 372
>sp|P38571|LICH_HUMAN Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 399
Score = 107 bits (267), Expect = 1e-23
Identities = 59/151 (39%), Positives = 89/151 (58%), Gaps = 1/151 (0%)
Frame = +1
Query: 40 NLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTP 219
+LFG + S K+L + +C L + C + FLL G + +NLN SR+ VY +H+P
Sbjct: 226 DLFGDKEFLPQSAFLKWLGTHVCTHVILKELCGNLCFLLCGFNERNLNMSRVDVYTTHSP 285
Query: 220 AGTSVMNIVHYAQSVDNGNFQMYDFGIEG-NIKRYGTPNPPQFDVKPVGIPTAIFSGGND 396
AGTSV N++H++Q+V FQ +D+G N Y PP ++VK + +PTA++SGG+D
Sbjct: 286 AGTSVQNMLHWSQAVKFQKFQAFDWGSSAKNYFHYNQSYPPTYNVKDMLVPTAVWSGGHD 345
Query: 397 TLADPEDVSRLLGLVGGDVRFQRFIRYYNHM 489
LAD DV+ LL + V F I + H+
Sbjct: 346 WLADVYDVNILLTQITNLV-FHESIPEWEHL 375
>sp|Q64194|LICH_RAT Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 106 bits (264), Expect = 3e-23
Identities = 54/134 (40%), Positives = 82/134 (61%), Gaps = 1/134 (0%)
Frame = +1
Query: 34 LLNLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISH 213
L +LFG + + S + K+L++ IC + + C+ + FL+ G + KNLN SR+ VY +H
Sbjct: 222 LEDLFGQKQFLPQSAMVKWLSTHICTHVIMKELCANIFFLICGFNEKNLNMSRVDVYTTH 281
Query: 214 TPAGTSVMNIVHYAQSVDNGNFQMYDFG-IEGNIKRYGTPNPPQFDVKPVGIPTAIFSGG 390
PAGTSV N+VH+ Q V Q +D+G + N Y PP + +K + +PTA++SGG
Sbjct: 282 CPAGTSVQNMVHWTQVVKYHKLQAFDWGSSDKNYFHYNQSYPPLYSIKDMQLPTALWSGG 341
Query: 391 NDTLADPEDVSRLL 432
D LAD D++ LL
Sbjct: 342 KDWLADTSDINILL 355
>sp|Q9Z0M5|LICH_MOUSE Lysosomal acid lipase/cholesteryl ester hydrolase precursor (LAL)
(Acid cholesteryl ester hydrolase) (Sterol esterase)
(Lipase A) (Cholesteryl esterase)
Length = 397
Score = 95.9 bits (237), Expect = 4e-20
Identities = 50/134 (37%), Positives = 78/134 (58%), Gaps = 1/134 (0%)
Frame = +1
Query: 34 LLNLFGHGRVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISH 213
L ++FG + + S + K+L+ +C + + C+ V FLL G + KNLN SR+ VY +H
Sbjct: 222 LKDMFGQKQFLPQSAMLKWLSIHVCTHVIMKELCANVFFLLCGFNEKNLNMSRVDVYTTH 281
Query: 214 TPAGTSVMNIVHYAQSVDNGNFQMYDFG-IEGNIKRYGTPNPPQFDVKPVGIPTAIFSGG 390
PA V N++H+ Q Q +D+G E N Y PP +++K + +PTA++SGG
Sbjct: 282 CPAELLVQNMLHWGQVFKYRKLQAFDWGSSEKNYFHYNQSFPPSYNIKNMRLPTALWSGG 341
Query: 391 NDTLADPEDVSRLL 432
D LAD D++ LL
Sbjct: 342 RDWLADINDITILL 355
>sp|O46107|LIP1_DROME Lipase 1 precursor (DmLip1)
Length = 439
Score = 74.3 bits (181), Expect = 1e-13
Identities = 44/147 (29%), Positives = 70/147 (47%), Gaps = 3/147 (2%)
Frame = +1
Query: 58 RVMSNSEIFKFLASTICGKEGLPKFCSEVIFLLAGHDIKNLNESRLPVYISHTPAGTSVM 237
R M N E F+FL E + C E +F + G + N PV + H PAG +
Sbjct: 254 REMFNGE-FRFLCRMT---EETERLCIEAVFGIVGRNWNEFNRKMFPVILGHYPAGVAAK 309
Query: 238 NIVHYAQSVDNGNFQMYDFGIEGNIKRYGTPNPPQFDVKPVGIPTAIFSGGNDTLADPED 417
+ H+ Q + +G F Y + N++ Y PP++++ V +PT ++ ND L P+D
Sbjct: 310 QVKHFIQIIKSGRFAPYSYSSNKNMQLYRDHLPPRYNLSLVTVPTFVYYSTNDLLCHPKD 369
Query: 418 VSRL---LGLVGGDVRFQRFIRYYNHM 489
V + LG V G + + +NHM
Sbjct: 370 VESMCDDLGNVTGKYLVPQ--KEFNHM 394
>sp|O46108|LIP3_DROME Lipase 3 precursor (DmLip3)
Length = 394
Score = 66.6 bits (161), Expect = 3e-11
Identities = 32/99 (32%), Positives = 53/99 (53%)
Frame = +1
Query: 133 CSEVIFLLAGHDIKNLNESRLPVYISHTPAGTSVMNIVHYAQSVDNGNFQMYDFGIEGNI 312
C+ IFL+ G+D + L+ L + +PAG SV +H+ Q ++G F+ +D+ N
Sbjct: 251 CANEIFLIGGYDTEQLDYELLEHIKATSPAGASVNQNLHFCQEYNSGKFRKFDYTALRNP 310
Query: 313 KRYGTPNPPQFDVKPVGIPTAIFSGGNDTLADPEDVSRL 429
YG+ PP + +K P ++ G ND + D DV +L
Sbjct: 311 YEYGSYFPPDYKLKNAKAPVLLYYGANDWMCDVSDVRKL 349
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 64,145,837
Number of Sequences: 369166
Number of extensions: 1417436
Number of successful extensions: 3806
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3681
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3798
length of database: 68,354,980
effective HSP length: 102
effective length of database: 49,512,010
effective search space used: 2970720600
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)