Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_P24
(671 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q94360|NUKM_CAEEL Probable NADH-ubiquinone oxidoreductas... 236 5e-62
sp|P42026|NUKM_BOVIN NADH-ubiquinone oxidoreductase 20 kDa ... 232 8e-61
sp|Q5R4J8|NUKM_PONPY NADH-ubiquinone oxidoreductase 20 kDa ... 231 2e-60
sp|O75251|NUKM_HUMAN NADH-ubiquinone oxidoreductase 20 kDa ... 230 2e-60
sp|Q9DC70|NUKM_MOUSE NADH-ubiquinone oxidoreductase 20 kDa ... 229 5e-60
sp|O21272|NUKM_RECAM NADH-ubiquinone oxidoreductase 20 kDa ... 224 2e-58
sp|Q42577|NUKM_ARATH NADH-ubiquinone oxidoreductase 20 kDa ... 224 2e-58
sp|Q43844|NUKM_SOLTU NADH-ubiquinone oxidoreductase 20 kDa ... 221 1e-57
sp|P42027|NUKM_BRAOL NADH-ubiquinone oxidoreductase 20 kDa ... 221 1e-57
sp|O47950|NUKM_NEUCR NADH-ubiquinone oxidoreductase 19.3 kD... 213 3e-55
>sp|Q94360|NUKM_CAEEL Probable NADH-ubiquinone oxidoreductase 20 kDa subunit,
mitochondrial precursor (Complex I-20KD) (CI-20KD)
Length = 199
Score = 236 bits (601), Expect = 5e-62
Identities = 113/164 (68%), Positives = 131/164 (79%)
Frame = +2
Query: 113 SPFQVPLSNKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDMDRFGLL 292
+PF P S+K ++ALAR DD++N ++GSIWP+TFGLACCAVEMMH AAPRYDMDR+G++
Sbjct: 37 TPFLNP-SSKAEYALARLDDVLNLAQRGSIWPLTFGLACCAVEMMHFAAPRYDMDRYGVV 95
Query: 293 FRASPRQADVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXXXXXXXX 472
FRASPRQAD+I VAGTV NKMA ALR+IYDQM KWVISMGSCAN
Sbjct: 96 FRASPRQADLIFVAGTVTNKMAPALRRIYDQMPEAKWVISMGSCANGGGYYHYAYSVLRG 155
Query: 473 CDRIIPVDIYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
CDR+IPVDIYVPGCPP+AEALLYG LQLQKKIKR Q+WYR+
Sbjct: 156 CDRVIPVDIYVPGCPPTAEALLYGVLQLQKKIKRKREAQLWYRR 199
>sp|P42026|NUKM_BOVIN NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD) (PSST subunit)
Length = 216
Score = 232 bits (591), Expect = 8e-61
Identities = 114/195 (58%), Positives = 143/195 (73%)
Frame = +2
Query: 20 LPMRNITVTTVKLQSKDTAPNVVDDQKPSPYSPFQVPLSNKFDFALARFDDLINFGRKGS 199
L +R + + T P V + P P +P S++ ++ +A+ DDLIN+ R+ S
Sbjct: 24 LQVRGVHSSMAADSPSSTQPAVSQARAVVP-KPAALP-SSRGEYVVAKLDDLINWARRSS 81
Query: 200 IWPMTFGLACCAVEMMHMAAPRYDMDRFGLLFRASPRQADVIIVAGTVCNKMATALRKIY 379
+WPMTFGLACCAVEMMHMAAPRYDMDRFG++FRASPRQ+DV+IVAGT+ NKMA ALRK+Y
Sbjct: 82 LWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQSDVMIVAGTLTNKMAPALRKVY 141
Query: 380 DQMYYPKWVISMGSCANXXXXXXXXXXXXXXCDRIIPVDIYVPGCPPSAEALLYGFLQLQ 559
DQM P++V+SMGSCAN CDRI+PVDIYVPGCPP+AEALLYG LQLQ
Sbjct: 142 DQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQ 201
Query: 560 KKIKRMDSIQMWYRK 604
KKIKR +++WYR+
Sbjct: 202 KKIKREKRLRIWYRR 216
>sp|Q5R4J8|NUKM_PONPY NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD) (PSST subunit)
Length = 213
Score = 231 bits (588), Expect = 2e-60
Identities = 105/157 (66%), Positives = 130/157 (82%)
Frame = +2
Query: 134 SNKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDMDRFGLLFRASPRQ 313
S++ ++ +A+ DDLIN+ R+ S+WPMTFGLACCAVEMMHMAAPRYDMDRFG++FRASPRQ
Sbjct: 57 SSRGEYVVAKLDDLINWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQ 116
Query: 314 ADVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXXXXXXXXCDRIIPV 493
+DV+IVAGT+ NKMA ALRK+YDQM P++V+SMGSCAN CDRI+PV
Sbjct: 117 SDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPV 176
Query: 494 DIYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
DIY+PGCPP+AEALLYG LQLQ+KIKR +Q+WYR+
Sbjct: 177 DIYIPGCPPTAEALLYGILQLQRKIKRERRLQIWYRR 213
>sp|O75251|NUKM_HUMAN NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD) (PSST subunit)
Length = 213
Score = 230 bits (587), Expect = 2e-60
Identities = 104/157 (66%), Positives = 130/157 (82%)
Frame = +2
Query: 134 SNKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDMDRFGLLFRASPRQ 313
S++ ++ +A+ DDL+N+ R+ S+WPMTFGLACCAVEMMHMAAPRYDMDRFG++FRASPRQ
Sbjct: 57 SSRGEYVVAKLDDLVNWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQ 116
Query: 314 ADVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXXXXXXXXCDRIIPV 493
+DV+IVAGT+ NKMA ALRK+YDQM P++V+SMGSCAN CDRI+PV
Sbjct: 117 SDVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPV 176
Query: 494 DIYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
DIY+PGCPP+AEALLYG LQLQ+KIKR +Q+WYR+
Sbjct: 177 DIYIPGCPPTAEALLYGILQLQRKIKRERRLQIWYRR 213
>sp|Q9DC70|NUKM_MOUSE NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD) (PSST subunit)
Length = 224
Score = 229 bits (584), Expect = 5e-60
Identities = 104/156 (66%), Positives = 128/156 (82%)
Frame = +2
Query: 137 NKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDMDRFGLLFRASPRQA 316
++ ++ + + DDLIN+ R+ S+WPMTFGLACCAVEMMHMAAPRYDMDRFG++FRASPRQA
Sbjct: 69 SRAEYVVTKLDDLINWARRSSLWPMTFGLACCAVEMMHMAAPRYDMDRFGVVFRASPRQA 128
Query: 317 DVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXXXXXXXXCDRIIPVD 496
DV+IVAGT+ NKMA ALRK+YDQM P++V+SMGSCAN CDRI+PVD
Sbjct: 129 DVMIVAGTLTNKMAPALRKVYDQMPEPRYVVSMGSCANGGGYYHYSYSVVRGCDRIVPVD 188
Query: 497 IYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
IYVPGCPP+AEALLYG LQLQ+KIKR +++WYR+
Sbjct: 189 IYVPGCPPTAEALLYGILQLQRKIKREQKLKIWYRR 224
>sp|O21272|NUKM_RECAM NADH-ubiquinone oxidoreductase 20 kDa subunit (NADH dehydrogenase
subunit 10)
Length = 182
Score = 224 bits (571), Expect = 2e-58
Identities = 103/156 (66%), Positives = 123/156 (78%)
Frame = +2
Query: 137 NKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDMDRFGLLFRASPRQA 316
NK +F +++ D+L+N+ RKGS+WPMTFGLACCAVEMMH AA RYD+DRFG++FR SPRQ+
Sbjct: 27 NKTEFIVSKMDELVNWARKGSLWPMTFGLACCAVEMMHSAASRYDLDRFGIIFRPSPRQS 86
Query: 317 DVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXXXXXXXXCDRIIPVD 496
DV+IVAGT+ NKMA ALRK+YDQM P+WV+SMGSCAN CDRI+PVD
Sbjct: 87 DVMIVAGTLTNKMAPALRKVYDQMSEPRWVVSMGSCANGGGYYHYSYSVVRGCDRIVPVD 146
Query: 497 IYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
IYVPGCPP+AEALLYG LQLQKKIKR W +K
Sbjct: 147 IYVPGCPPTAEALLYGLLQLQKKIKRSRKTLYWLQK 182
>sp|Q42577|NUKM_ARATH NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD)
Length = 218
Score = 224 bits (570), Expect = 2e-58
Identities = 109/187 (58%), Positives = 129/187 (68%)
Frame = +2
Query: 44 TTVKLQSKDTAPNVVDDQKPSPYSPFQVPLSNKFDFALARFDDLINFGRKGSIWPMTFGL 223
T++ S T+P P SP LS +F +++ DDL+N+ R GSIWPMTFGL
Sbjct: 32 TSLPALSPSTSPTSYTRPGPPSTSPPPPGLSKAAEFVISKVDDLMNWARTGSIWPMTFGL 91
Query: 224 ACCAVEMMHMAAPRYDMDRFGLLFRASPRQADVIIVAGTVCNKMATALRKIYDQMYYPKW 403
ACCAVEMMH A RYD+DRFG++FR SPRQ+D +IVAGT+ NKMA ALRK+YDQM P+W
Sbjct: 92 ACCAVEMMHTGAARYDLDRFGIIFRPSPRQSDCMIVAGTLTNKMAPALRKVYDQMPEPRW 151
Query: 404 VISMGSCANXXXXXXXXXXXXXXCDRIIPVDIYVPGCPPSAEALLYGFLQLQKKIKRMDS 583
VISMGSCAN CDRI+PVDIYVPGCPP+AEALLYG LQLQKKI R
Sbjct: 152 VISMGSCANGGGYYHYSYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLLQLQKKINRRKD 211
Query: 584 IQMWYRK 604
W+ K
Sbjct: 212 FLHWWNK 218
>sp|Q43844|NUKM_SOLTU NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD)
Length = 213
Score = 221 bits (564), Expect = 1e-57
Identities = 105/168 (62%), Positives = 126/168 (75%)
Frame = +2
Query: 95 QKPSPYSPFQVPLSNKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDM 274
Q PS +P +S ++ +++ DDL+N+ R+GSIWPMTFGLACCAVEMMH A RYD
Sbjct: 46 QPPSMNTP--AGISKPAEYVISKVDDLMNWARRGSIWPMTFGLACCAVEMMHAGAARYDF 103
Query: 275 DRFGLLFRASPRQADVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXX 454
DRFG++FR SPRQ+DV+IVAGT+ NKMA ALRK+YDQM P+WVISMGSCAN
Sbjct: 104 DRFGIIFRPSPRQSDVMIVAGTLTNKMAPALRKVYDQMPEPRWVISMGSCANGGGYYHYS 163
Query: 455 XXXXXXCDRIIPVDIYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWY 598
CDRI+PVDIYVPGCPP+AEALLYG LQLQKKI R + MW+
Sbjct: 164 YAVVRGCDRIVPVDIYVPGCPPTAEALLYGILQLQKKINRRKDLLMWW 211
>sp|P42027|NUKM_BRAOL NADH-ubiquinone oxidoreductase 20 kDa subunit, mitochondrial
precursor (Complex I-20KD) (CI-20KD)
Length = 215
Score = 221 bits (563), Expect = 1e-57
Identities = 106/171 (61%), Positives = 125/171 (73%), Gaps = 2/171 (1%)
Frame = +2
Query: 98 KPSPYSPFQVP--LSNKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYD 271
+P P S P LS +F +++ DDL+N+ R+GSIWPMTFGLACCAVEMMH A RYD
Sbjct: 45 RPGPPSTSAPPPGLSKTAEFVISKVDDLMNWARRGSIWPMTFGLACCAVEMMHTGAARYD 104
Query: 272 MDRFGLLFRASPRQADVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXX 451
+DRFG++FR SPRQ+D +IVAGT+ NKMA ALRK+YDQM P+WVISMGSCAN
Sbjct: 105 LDRFGIIFRPSPRQSDCMIVAGTLTNKMAPALRKVYDQMPEPRWVISMGSCANGGGYYHY 164
Query: 452 XXXXXXXCDRIIPVDIYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
CDRI+PVDIYVPGCPP+AEALLYG LQLQKKI R W+ K
Sbjct: 165 SYSVVRGCDRIVPVDIYVPGCPPTAEALLYGLLQLQKKINRRKDFLHWWNK 215
>sp|O47950|NUKM_NEUCR NADH-ubiquinone oxidoreductase 19.3 kDa subunit, mitochondrial
precursor (Complex I-19.3KD) (CI-19.3KD)
Length = 226
Score = 213 bits (543), Expect = 3e-55
Identities = 96/163 (58%), Positives = 120/163 (73%)
Frame = +2
Query: 116 PFQVPLSNKFDFALARFDDLINFGRKGSIWPMTFGLACCAVEMMHMAAPRYDMDRFGLLF 295
P Q +AL D ++N+ R+ S+WPMTFGLACCAVEMMH++ PRYD DR G++F
Sbjct: 64 PSQEGTKGAVQYALTTLDSIVNWARQSSLWPMTFGLACCAVEMMHLSTPRYDQDRLGIIF 123
Query: 296 RASPRQADVIIVAGTVCNKMATALRKIYDQMYYPKWVISMGSCANXXXXXXXXXXXXXXC 475
RASPRQ+DV+IVAGT+ NKMA ALR++YDQM P+WVISMGSCAN C
Sbjct: 124 RASPRQSDVMIVAGTLTNKMAPALRQVYDQMPDPRWVISMGSCANGGGYYHYSYSVVRGC 183
Query: 476 DRIIPVDIYVPGCPPSAEALLYGFLQLQKKIKRMDSIQMWYRK 604
DRI+PVDIYVPGCPP++EAL+YG QLQ+K++ +MWYRK
Sbjct: 184 DRIVPVDIYVPGCPPTSEALMYGIFQLQRKMRNTKITRMWYRK 226
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,532,844
Number of Sequences: 369166
Number of extensions: 1485551
Number of successful extensions: 3530
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3397
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3488
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 5636246860
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)