Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_P07
(805 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q10580|SWAP_CAEEL SWAP protein (Suppressor of white apri... 77 7e-14
sp|P12297|SUWA_DROME Suppressor of white apricot protein 73 8e-13
sp|Q12872|SFRS8_HUMAN Splicing factor, arginine/serine-rich... 73 1e-12
sp|Q8K4Z5|SF3A1_MOUSE Splicing factor 3 subunit 1 (SF3a120) 52 2e-06
sp|Q15459|SF3A1_HUMAN Splicing factor 3 subunit 1 (Spliceos... 51 4e-06
sp|Q68FU8|SF04_RAT Splicing factor 4 40 0.007
sp|Q8CH02|SF04_MOUSE Splicing factor 4 39 0.013
sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein... 39 0.017
sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1 37 0.082
sp|Q7ZUS4|ST2S2_BRARE Cytosolic sulfotransferase 2 (SULT1 ST2) 34 0.53
>sp|Q10580|SWAP_CAEEL SWAP protein (Suppressor of white apricot protein homolog)
Length = 749
Score = 76.6 bits (187), Expect = 7e-14
Identities = 44/123 (35%), Positives = 66/123 (53%), Gaps = 15/123 (12%)
Frame = +1
Query: 16 DYDNNQEKNESQESKYE--------------KEVEDNGTDAAYLCPPNLKLLPGMITPAT 153
D QEK E ++ + + K E + D + P +K G+ P+
Sbjct: 101 DIQREQEKEEEEKRRNDQRNAIGFDYGTGKVKARESDSEDEPFEPPEGIKFPVGLELPSN 160
Query: 154 EREAAIIEQTAKFVADKGPQLEILIKAKQSNN-PKFVFLNFDHKLYPFYRHVTQLIKTAQ 330
+ IIE+TA F+ G Q+EI+IKAKQ NN +F FL FDH+L PFY+++ +LI+ +
Sbjct: 161 MKLHHIIEKTASFIVANGTQMEIVIKAKQRNNAEQFGFLEFDHRLNPFYKYLQKLIREKK 220
Query: 331 YIP 339
YIP
Sbjct: 221 YIP 223
Score = 34.3 bits (77), Expect = 0.41
Identities = 42/164 (25%), Positives = 70/164 (42%), Gaps = 10/164 (6%)
Frame = +1
Query: 37 KNESQESKYEKEVEDNGTDAAYL----------CPPNLKLLPGMITPATEREAAIIEQTA 186
K +E+K EKE D+ D Y CP + P + PAT I+ A
Sbjct: 341 KMNVEEAKKEKE-NDHLDDPEYREWYENFYGRPCP---WIGPRPMIPATPDLEPILNSYA 396
Query: 187 KFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIKTAQYIPGKRHISHLV 366
+ VA +G + E + A++ + + F+ Y +Y H ++ + Y P ++++S LV
Sbjct: 397 EHVAQRGLEAEASLAARE--DLQLHFMEPKSPYYSYYHHKVRMHQWRMYQPIEQNLSPLV 454
Query: 367 ESNGDQSQMGSFPQPVAPKIDISSTPYGKLIERFKQSQEKKKLD 498
++ S P P + STP L R Q ++ LD
Sbjct: 455 LNSPAPPSAVSSPGPSSLMSLNLSTPEPPLNRR----QRRRLLD 494
>sp|P12297|SUWA_DROME Suppressor of white apricot protein
Length = 963
Score = 73.2 bits (178), Expect = 8e-13
Identities = 38/86 (44%), Positives = 56/86 (65%), Gaps = 2/86 (2%)
Frame = +1
Query: 88 TDAAYLCPPNLKLLP--GMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFV 261
++ ++ P L + P M P T ++ AIIE+TA+F+A +G Q+EILIKAKQ+NN +F
Sbjct: 205 SELPFVLPYTLMMAPPLDMQLPETMKQHAIIEKTARFIATQGAQMEILIKAKQANNTQFD 264
Query: 262 FLNFDHKLYPFYRHVTQLIKTAQYIP 339
FL L P+YRH+ IK A++ P
Sbjct: 265 FLTQGGHLQPYYRHLLAAIKAAKFPP 290
>sp|Q12872|SFRS8_HUMAN Splicing factor, arginine/serine-rich 8 (Suppressor of white
apricot protein homolog)
Length = 951
Score = 72.8 bits (177), Expect = 1e-12
Identities = 40/112 (35%), Positives = 64/112 (57%), Gaps = 6/112 (5%)
Frame = +1
Query: 16 DYDNNQEKNESQESKYEKE------VEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIE 177
DY + E E +E ++E +E+N + ++ P L + + P T + AIIE
Sbjct: 156 DYYDPSEPTEEEEPSKQREKNEAENLEEN--EEPFVAPLGLSVPSDVELPPTAKMHAIIE 213
Query: 178 QTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIKTAQY 333
+TA FV +G Q EI++KAKQ+ N +F FL FDH L P+Y+ + + +K +Y
Sbjct: 214 RTASFVCRQGAQFEIMLKAKQAPNSQFDFLRFDHYLNPYYKFIQKAMKEGRY 265
>sp|Q8K4Z5|SF3A1_MOUSE Splicing factor 3 subunit 1 (SF3a120)
Length = 791
Score = 51.6 bits (122), Expect = 2e-06
Identities = 33/106 (31%), Positives = 51/106 (48%)
Frame = +1
Query: 109 PPNLKLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLY 288
P K + G+I P E I+++TA FVA GP+ E I+ + NNPKF FLN + +
Sbjct: 33 PTPSKPVVGIIYPPPEVRN-IVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYH 91
Query: 289 PFYRHVTQLIKTAQYIPGKRHISHLVESNGDQSQMGSFPQPVAPKI 426
+YRH K + I +++ Q+ PQ V ++
Sbjct: 92 AYYRHKVSEFKEGKAQEPSAAIPKVMQQQ-QQATQQQLPQKVQAQV 136
Score = 33.1 bits (74), Expect = 0.91
Identities = 29/110 (26%), Positives = 52/110 (47%), Gaps = 2/110 (1%)
Frame = +1
Query: 169 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIK--TAQYIPG 342
+++ TA+FVA G Q + K+ N +F FL H L+ ++ T+L++ T IP
Sbjct: 166 VVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYF---TKLVEQYTKILIPP 222
Query: 343 KRHISHLVESNGDQSQMGSFPQPVAPKIDISSTPYGKLIERFKQSQEKKK 492
K S L + P+ V ++ + K ER ++ +E++K
Sbjct: 223 KGLFSKL-------KKEAENpreVLDQV-CYRVEWAKFQERERKKEEEEK 264
>sp|Q15459|SF3A1_HUMAN Splicing factor 3 subunit 1 (Spliceosome associated protein 114)
(SAP 114) (SF3a120)
Length = 793
Score = 50.8 bits (120), Expect = 4e-06
Identities = 32/102 (31%), Positives = 50/102 (49%)
Frame = +1
Query: 121 KLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYR 300
K + G+I P E I+++TA FVA GP+ E I+ + NNPKF FLN + + +YR
Sbjct: 37 KPVVGIIYPPPEVRN-IVDKTASFVARNGPEFEARIRQNEINNPKFNFLNPNDPYHAYYR 95
Query: 301 HVTQLIKTAQYIPGKRHISHLVESNGDQSQMGSFPQPVAPKI 426
H K + I +++ Q+ PQ V ++
Sbjct: 96 HKVSEFKEGKAQEPSAAIPKVMQQQ-QQTTQQQLPQKVQAQV 136
Score = 33.1 bits (74), Expect = 0.91
Identities = 29/110 (26%), Positives = 52/110 (47%), Gaps = 2/110 (1%)
Frame = +1
Query: 169 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFYRHVTQLIK--TAQYIPG 342
+++ TA+FVA G Q + K+ N +F FL H L+ ++ T+L++ T IP
Sbjct: 166 VVKLTAQFVARNGRQFLTQLMQKEQRNYQFDFLRPQHSLFNYF---TKLVEQYTKILIPP 222
Query: 343 KRHISHLVESNGDQSQMGSFPQPVAPKIDISSTPYGKLIERFKQSQEKKK 492
K S L + P+ V ++ + K ER ++ +E++K
Sbjct: 223 KGLFSKL-------KKEAENpreVLDQV-CYRVEWAKFQERERKKEEEEK 264
>sp|Q68FU8|SF04_RAT Splicing factor 4
Length = 644
Score = 40.0 bits (92), Expect = 0.007
Identities = 40/164 (24%), Positives = 67/164 (40%), Gaps = 18/164 (10%)
Frame = +1
Query: 7 ARGDYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAA 168
A DY +N +KN + Y K+V + +A K+ P P E
Sbjct: 207 AMEDYKDNPAFTFLHDKNSREFLYYRKKVAEIRKEAQKPQAATQKVSP----PEDEEAKN 262
Query: 169 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQY---- 333
+ E+ A+F+AD GP++E + N F FL + + + Y +Y+ + + A+
Sbjct: 263 LAEKLARFIADGGPEVETIALQNNRENQAFSFLYDPNSQGYRYYKQKLEEFRKAKAGSTG 322
Query: 334 -------IPGKRHISHLVESNGDQSQMGSFPQPVAPKIDISSTP 444
P R S +G + + P PVAP ++ TP
Sbjct: 323 SLPAPVPNPSLRRKSAPEALSGAVPPITACPTPVAPAPAVNPTP 366
Score = 36.6 bits (83), Expect = 0.082
Identities = 21/56 (37%), Positives = 35/56 (62%), Gaps = 2/56 (3%)
Frame = +1
Query: 169 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYR-HVTQLIKTAQ 330
+IE+ A+FVA+ GP+LE + +NP F FL + + + + +YR V ++ K AQ
Sbjct: 188 VIEKLARFVAEGGPELEKVAMEDYKDNPAFTFLHDKNSREFLYYRKKVAEIRKEAQ 243
>sp|Q8CH02|SF04_MOUSE Splicing factor 4
Length = 643
Score = 39.3 bits (90), Expect = 0.013
Identities = 40/164 (24%), Positives = 66/164 (40%), Gaps = 18/164 (10%)
Frame = +1
Query: 7 ARGDYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAA 168
A DY +N +KN + Y ++V + +A K+ P P E
Sbjct: 206 AMEDYKDNPAFTFLHDKNSREFLYYRRKVAEIRKEAQKPQAATQKVSP----PEDEEAKN 261
Query: 169 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQY---- 333
+ E+ A+F+AD GP++E + N F FL + + + Y +YR + A+
Sbjct: 262 LAEKLARFIADGGPEVETIALQNNRENQAFSFLYDPNSQGYRYYRQKLDEFRKAKAGSTG 321
Query: 334 -------IPGKRHISHLVESNGDQSQMGSFPQPVAPKIDISSTP 444
P R S +G + + P PVAP ++ TP
Sbjct: 322 SFPAPAPNPSLRRKSAPEALSGAVPPITACPTPVAPAPAVNPTP 365
Score = 38.5 bits (88), Expect = 0.022
Identities = 31/103 (30%), Positives = 48/103 (46%), Gaps = 3/103 (2%)
Frame = +1
Query: 31 QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP 210
Q ++ +E YE+ +E +K+ P P +IE+ A+FVA+ GP
Sbjct: 158 QSPDDDEEEDYEQWLE-------------IKVSP----PEGAETRRVIEKLARFVAEGGP 200
Query: 211 QLEILIKAKQSNNPKFVFL---NFDHKLYPFYRHVTQLIKTAQ 330
+LE + +NP F FL N LY + R V ++ K AQ
Sbjct: 201 ELEKVAMEDYKDNPAFTFLHDKNSREFLY-YRRKVAEIRKEAQ 242
>sp|Q8IWZ8|SF04_HUMAN Splicing factor 4 (RNA-binding protein RBP)
Length = 645
Score = 38.9 bits (89), Expect = 0.017
Identities = 30/102 (29%), Positives = 50/102 (49%), Gaps = 2/102 (1%)
Frame = +1
Query: 31 QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGP 210
Q +E +E YE+ +E +K+ P P +IE+ A+FVA+ GP
Sbjct: 162 QSPDEDEEEDYEQWLE-------------IKVSP----PEGAETRKVIEKLARFVAEGGP 204
Query: 211 QLEILIKAKQSNNPKFVFL-NFDHKLYPFYR-HVTQLIKTAQ 330
+LE + +NP F FL + + + + +YR V ++ K AQ
Sbjct: 205 ELEKVAMEDYKDNPAFAFLHDKNSREFLYYRKKVAEIRKEAQ 246
Score = 37.7 bits (86), Expect = 0.037
Identities = 30/115 (26%), Positives = 51/115 (44%), Gaps = 7/115 (6%)
Frame = +1
Query: 7 ARGDYDNN------QEKNESQESKYEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAA 168
A DY +N +KN + Y K+V + +A + K+ P P E
Sbjct: 210 AMEDYKDNPAFAFLHDKNSREFLYYRKKVAEIRKEAQKSQAASQKVSP----PEDEEVKN 265
Query: 169 IIEQTAKFVADKGPQLEILIKAKQSNNPKFVFL-NFDHKLYPFYRHVTQLIKTAQ 330
+ E+ A+F+AD GP++E + N F FL + + Y +YR + + A+
Sbjct: 266 LAEKLARFIADGGPEVETIALQNNRENQAFSFLYEPNSQGYKYYRQKLEEFRKAK 320
>sp|Q8RXF1|SF3A1_ARATH Probable splicing factor 3 subunit 1
Length = 785
Score = 36.6 bits (83), Expect = 0.082
Identities = 17/49 (34%), Positives = 28/49 (57%)
Frame = +1
Query: 151 TEREAAIIEQTAKFVADKGPQLEILIKAKQSNNPKFVFLNFDHKLYPFY 297
T E II+ TA+FVA G + +++NNP+F F+ H ++ F+
Sbjct: 187 TGEELDIIKLTAQFVARNGKSFLTGLSNRENNNPQFHFMKPTHSMFTFF 235
>sp|Q7ZUS4|ST2S2_BRARE Cytosolic sulfotransferase 2 (SULT1 ST2)
Length = 301
Score = 33.9 bits (76), Expect = 0.53
Identities = 26/80 (32%), Positives = 36/80 (45%)
Frame = +1
Query: 61 YEKEVEDNGTDAAYLCPPNLKLLPGMITPATEREAAIIEQTAKFVADKGPQLEILIKAKQ 240
YE VED G + A LC G+ T ATERE KG Q +++ + K
Sbjct: 201 YEDMVEDTGREVARLCS-----FLGLSTSATERER----------ITKGVQFDVMKQNKM 245
Query: 241 SNNPKFVFLNFDHKLYPFYR 300
+N ++F K+ PF R
Sbjct: 246 TNYSTLPVMDF--KISPFMR 263
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.310 0.128 0.357
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 90,821,978
Number of Sequences: 369166
Number of extensions: 1790960
Number of successful extensions: 3983
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3854
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3981
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 7618580670
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)