Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_O23
(719 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD4... 69 2e-11
sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD4... 69 2e-11
sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Int... 61 3e-09
sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6 59 1e-08
sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RIN... 58 2e-08
sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5 57 4e-08
sp|Q13077|TRAF1_HUMAN TNF receptor-associated factor 1 (Eps... 56 8e-08
sp|P39428|TRAF1_MOUSE TNF receptor-associated factor 1 54 3e-07
sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cys... 53 7e-07
sp|Q12933|TRAF2_HUMAN TNF receptor-associated factor 2 (Tum... 53 7e-07
>sp|Q13114|TRAF3_HUMAN TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (CD40 binding protein) (CD40BP) (LMP1
associated protein) (LAP1) (CAP-1)
Length = 568
Score = 68.6 bits (166), Expect = 2e-11
Identities = 49/197 (24%), Positives = 91/197 (46%), Gaps = 12/197 (6%)
Frame = +1
Query: 4 DEMKGKCEQTENKEIEMRRIIDEIFD--KNADLMKKSNIDYDEKINNLQIKIIEME---R 168
D MK E +N+ E+ + +N L++ +D+ ++ I++ +M+ +
Sbjct: 348 DSMKSSVESLQNRVTELESVDKSAGQVARNTGLLESQLSRHDQMLSVHDIRLADMDLRFQ 407
Query: 169 ITEKINF-GEFEWKIKNFSQQQRKT------DIFSESFYSYKNGYKMCLSVFIDCVCVYC 327
+ E ++ G WKI+++ +++++ ++S+ FY+ GYKMC V+++
Sbjct: 408 VLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLN------ 461
Query: 328 LSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDIINQTSGGVYKSKTIKY 507
F +MRGE+D L WPFK VT+ +++Q S + K
Sbjct: 462 -------GDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKP 514
Query: 508 DDYPNKKGWNKPTTERN 558
D PN + KPT E N
Sbjct: 515 D--PNSSSFKKPTGEMN 529
>sp|Q60803|TRAF3_MOUSE TNF receptor-associated factor 3 (CD40 receptor associated factor
1) (CRAF1) (TRAFAMN)
Length = 567
Score = 68.6 bits (166), Expect = 2e-11
Identities = 49/197 (24%), Positives = 91/197 (46%), Gaps = 12/197 (6%)
Frame = +1
Query: 4 DEMKGKCEQTENKEIEMRRIIDEIFD--KNADLMKKSNIDYDEKINNLQIKIIEME---R 168
D MK E +N+ E+ + +N L++ +D+ ++ I++ +M+ +
Sbjct: 347 DSMKSSVESLQNRVTELESVDKSAGQAARNTGLLESQLSRHDQTLSVHDIRLADMDLRFQ 406
Query: 169 ITEKINF-GEFEWKIKNFSQQQRKT------DIFSESFYSYKNGYKMCLSVFIDCVCVYC 327
+ E ++ G WKI+++ +++++ ++S+ FY+ GYKMC V+++
Sbjct: 407 VLETASYNGVLIWKIRDYKRRKQEAVMGKTLSLYSQPFYTGYFGYKMCARVYLN------ 460
Query: 328 LSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDIINQTSGGVYKSKTIKY 507
F +MRGE+D L WPFK VT+ +++Q S + K
Sbjct: 461 -------GDGMGKGTHLSLFFVIMRGEYDALLPWPFKQKVTLMLMDQGSSRRHLGDAFKP 513
Query: 508 DDYPNKKGWNKPTTERN 558
D PN + KPT E N
Sbjct: 514 D--PNSSSFKKPTGEMN 528
>sp|Q9Y4K3|TRAF6_HUMAN TNF receptor-associated factor 6 (Interleukin 1 signal transducer)
(RING finger protein 85)
Length = 522
Score = 61.2 bits (147), Expect = 3e-09
Identities = 48/190 (25%), Positives = 88/190 (46%), Gaps = 18/190 (9%)
Frame = +1
Query: 130 INNLQIKIIEMERITEKINFGEFEWKIKNFS------QQQRKTDIFSESFYSYKNGYKMC 291
I L+ K+ E+E ++ N G + WKI NF ++++ I S FY+ K GYK+C
Sbjct: 334 IRTLEDKVAEIE--AQQCN-GIYIWKIGNFGMHLKCQEEEKPVVIHSPGFYTGKPGYKLC 390
Query: 292 LSVFIDCV----CVYCLSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDI 459
+ + + C +S H M+GE+D+ L WPF+ + + I
Sbjct: 391 MRLHLQLPTAQRCANYISLFV----------------HTMQGEYDSHLPWPFQGTIRLTI 434
Query: 460 INQTSGGVYKSKTIKYDDYPNKKGWNKPTTERN----DYICFHRI--IIPIKITRNNQLL 621
++Q+ V ++ D P + +PT RN Y+ F + + +++ LL
Sbjct: 435 LDQSEAPVRQNHEEIMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQRTFIKDDTLL 494
Query: 622 IKCKI--KFD 645
++C++ +FD
Sbjct: 495 VRCEVSTRFD 504
>sp|P70196|TRAF6_MOUSE TNF receptor-associated factor 6
Length = 530
Score = 59.3 bits (142), Expect = 1e-08
Identities = 47/190 (24%), Positives = 87/190 (45%), Gaps = 18/190 (9%)
Frame = +1
Query: 130 INNLQIKIIEMERITEKINFGEFEWKIKNFS------QQQRKTDIFSESFYSYKNGYKMC 291
I L+ K+ EME ++ N G + WKI F +++R I S FY+ + GYK+C
Sbjct: 342 IRTLEDKVAEME--AQQCN-GIYIWKIGKFGMHLKSQEEERPVVIHSPGFYTGRPGYKLC 398
Query: 292 LSVFIDCV----CVYCLSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDI 459
+ + + C +S H M+GE+D+ L WPF+ + + I
Sbjct: 399 MRLHLQLPTAQRCANYISLFV----------------HTMQGEYDSHLPWPFQGTIRLTI 442
Query: 460 INQTSGGVYKSKTIKYDDYPNKKGWNKPTTERN----DYICFHRI--IIPIKITRNNQLL 621
++Q+ + ++ D P + +PT RN Y+ F + + +++ LL
Sbjct: 443 LDQSEALIRQNHEEVMDAKPELLAFQRPTIPRNPKGFGYVTFMHLEALRQGTFIKDDTLL 502
Query: 622 IKCKI--KFD 645
++C++ +FD
Sbjct: 503 VRCEVSTRFD 512
>sp|O00463|TRAF5_HUMAN TNF receptor-associated factor 5 (RING finger protein 84)
Length = 557
Score = 58.2 bits (139), Expect = 2e-08
Identities = 37/129 (28%), Positives = 62/129 (48%), Gaps = 6/129 (4%)
Frame = +1
Query: 190 GEFEWKIKNFSQQQRKT------DIFSESFYSYKNGYKMCLSVFIDCVCVYCLSXXXXXX 351
G+ WK+ ++ ++R+ IFS+SFY+ + GY++C +++
Sbjct: 404 GKLIWKVTDYKMKKREAVDGHTVSIFSQSFYTSRCGYRLCARAYLN-------------G 450
Query: 352 XXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDIINQTSGGVYKSKTIKYDDYPNKKG 531
F +MRGEFD+ L WPF+ VT+ +++Q SG +T K D PN
Sbjct: 451 DGSGRGSHLSLYFVVMRGEFDSLLQWPFRQRVTLMLLDQ-SGKKNIMETFKPD--PNSSS 507
Query: 532 WNKPTTERN 558
+ +P E N
Sbjct: 508 FKRPDGEMN 516
>sp|P70191|TRAF5_MOUSE TNF receptor-associated factor 5
Length = 558
Score = 57.4 bits (137), Expect = 4e-08
Identities = 40/173 (23%), Positives = 79/173 (45%), Gaps = 10/173 (5%)
Frame = +1
Query: 70 EIFDKNADLMKKSNIDYDEKINNLQIKIIEMERITEKIN----FGEFEWKIKNFSQQQRK 237
E D+ L++ +D IN + ++ + E +++ G+ WK+ ++ ++R+
Sbjct: 360 EASDQRLVLLEGETSKHDAHINIHKAQLNKNEERFKQLEGACYSGKLIWKVTDYRVKKRE 419
Query: 238 T------DIFSESFYSYKNGYKMCLSVFIDCVCVYCLSXXXXXXXXXXXXXXXXXXFHLM 399
+FS+ FY+ + GY++C +++ F +M
Sbjct: 420 AVEGHTVSVFSQPFYTSRCGYRLCARAYLN-------------GDGSGKGTHLSLYFVVM 466
Query: 400 RGEFDNDLLWPFKHAVTIDIINQTSGGVYKSKTIKYDDYPNKKGWNKPTTERN 558
RGEFD+ L WPF+ VT+ +++Q+ + +T K D PN + +P E N
Sbjct: 467 RGEFDSLLQWPFRQRVTLMLLDQSGKKNHIVETFKAD--PNSSSFKRPDGEMN 517
>sp|Q13077|TRAF1_HUMAN TNF receptor-associated factor 1 (Epstein-Barr virus-induced
protein 6)
Length = 416
Score = 56.2 bits (134), Expect = 8e-08
Identities = 44/180 (24%), Positives = 83/180 (46%), Gaps = 24/180 (13%)
Frame = +1
Query: 7 EMKGKCEQTENKEIEMRRIIDEIFDKNADLMKKSNIDYDEKINNLQIKIIEME------- 165
E++GK EN + + ++ A + +S +D E+I +L+ +++E++
Sbjct: 189 ELEGKLRVFENIVAVLNKEVEASHLALATSIHQSQLDR-ERILSLEQRVVELQQTLAQKD 247
Query: 166 ----------RITEKINF-GEFEWKIKNFSQQ------QRKTDIFSESFYSYKNGYKMCL 294
R+ E+ +F G F WKI N +++ R +FS +FY+ K GYK+CL
Sbjct: 248 QALGKLEQSLRLMEEASFDGTFLWKITNVTRRCHESACGRTVSLFSPAFYTAKYGYKLCL 307
Query: 295 SVFIDCVCVYCLSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDIINQTS 474
++++ +MRGE+D L WPF++ VT +++Q +
Sbjct: 308 RLYLN-------------GDGTGKRTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNN 354
>sp|P39428|TRAF1_MOUSE TNF receptor-associated factor 1
Length = 409
Score = 54.3 bits (129), Expect = 3e-07
Identities = 37/163 (22%), Positives = 72/163 (44%), Gaps = 17/163 (10%)
Frame = +1
Query: 37 NKEIEMRRIIDEIFDKNADLMKKSNIDYDEKINNLQIKIIEMERITEKINF--------- 189
NKE+E + + L ++ + ++++ LQ + + +++ K+
Sbjct: 198 NKEVEASHLALAASIHQSQLDREHLLSLEQRVVELQQTLAQKDQVLGKLEHSLRLMEEAS 257
Query: 190 --GEFEWKIKNFSQQ------QRKTDIFSESFYSYKNGYKMCLSVFIDCVCVYCLSXXXX 345
G F WKI N +++ R +FS +FY+ K GYK+CL ++++
Sbjct: 258 FDGTFLWKITNVTKRCHESVCGRTVSLFSPAFYTAKYGYKLCLRLYLN------------ 305
Query: 346 XXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDIINQTS 474
+MRGE+D L WPF++ VT +++Q +
Sbjct: 306 -GDGSGKKTHLSLFIVIMRGEYDALLPWPFRNKVTFMLLDQNN 347
>sp|Q9BUZ4|TRAF4_HUMAN TNF receptor-associated factor 4 (Cysteine-rich domain associated
with RING and Traf domains protein 1) (Malignant 62)
(RING finger protein 83)
Length = 470
Score = 53.1 bits (126), Expect = 7e-07
Identities = 38/144 (26%), Positives = 66/144 (45%), Gaps = 11/144 (7%)
Frame = +1
Query: 157 EMERITEKINFGE---FEWKIKNFSQQQRKT------DIFSESFYSYKNGYKMCLSVFID 309
E+ R E+++ G WKI ++ ++ ++ + FS +FY++K GYK+ +S F++
Sbjct: 294 ELRRELEELSVGSDGVLIWKIGSYGRRLQEAKAKPNLECFSPAFYTHKYGYKLQVSAFLN 353
Query: 310 CVCVYCLSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDIINQTSGGVYK 489
++ G FDN L WPF VT +++Q+ G+ K
Sbjct: 354 -------------GNGSGEGTHLSLYIRVLPGAFDNLLEWPFARRVTFSLLDQSDPGLAK 400
Query: 490 SK--TIKYDDYPNKKGWNKPTTER 555
+ T + PN K + KP T R
Sbjct: 401 PQHVTETFHPDPNWKNFQKPGTWR 424
>sp|Q12933|TRAF2_HUMAN TNF receptor-associated factor 2 (Tumor necrosis factor type 2
receptor-associated protein 3)
Length = 501
Score = 53.1 bits (126), Expect = 7e-07
Identities = 35/124 (28%), Positives = 61/124 (49%), Gaps = 6/124 (4%)
Frame = +1
Query: 121 DEKINNLQIKIIEMERITEKINFGEFEWKIKNFSQQQRKT------DIFSESFYSYKNGY 282
D + +L+ K++EME T G F WKI +F++++++ IFS +FY+ + GY
Sbjct: 332 DLAMADLEQKVLEMEASTYD---GVFIWKISDFARKRQEAVAGRIPAIFSPAFYTSRYGY 388
Query: 283 KMCLSVFIDCVCVYCLSXXXXXXXXXXXXXXXXXXFHLMRGEFDNDLLWPFKHAVTIDII 462
KMCL ++++ F +M+G D L WPF VT+ ++
Sbjct: 389 KMCLRIYLN-------------GDGTGRGTHLSLFFVVMKGPNDALLRWPFNQKVTLMLL 435
Query: 463 NQTS 474
+Q +
Sbjct: 436 DQNN 439
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 62,876,738
Number of Sequences: 369166
Number of extensions: 1040746
Number of successful extensions: 3225
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3099
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3212
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6413660220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)