Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_O10
(720 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P32369|BAIG_EUBSP Bile acid transporter 51 3e-06
sp|P37643|YHJE_ECOLI Inner membrane metabolite transport pr... 48 2e-05
sp|P46333|CSBC_BACSU Probable metabolite transport protein ... 48 2e-05
sp|Q92EE1|LMRB_LISIN Lincomycin resistance protein lmrB 46 1e-04
sp|Q8Y9K8|LMRB_LISMO Lincomycin resistance protein lmrB 46 1e-04
sp|P46907|NARK_BACSU Nitrite extrusion protein (Nitrite fac... 45 1e-04
sp|P47234|LACY_CITFR Lactose permease (Lactose-proton symport) 44 3e-04
sp|Q58955|YF60_METJA Putative transporter MJ1560 43 7e-04
sp|P58120|PMRA_LACLA Multi-drug resistance efflux pump pmrA... 42 0.002
sp|Q57QK4|MDTG_SALCH Multidrug resistance protein mdtG 41 0.004
>sp|P32369|BAIG_EUBSP Bile acid transporter
Length = 477
Score = 51.2 bits (121), Expect = 3e-06
Identities = 33/144 (22%), Positives = 67/144 (46%), Gaps = 1/144 (0%)
Frame = +2
Query: 65 GLNGADAALITSLLYIVSAACSPVMGFLIDLTGRNLFWVLTGTLATLGS-HALYAFTFAN 241
G++ + I+ L + SAAC+P++G L D+ GR +L + G+ A +
Sbjct: 42 GIDYNNTTWISLALAMSSAACAPILGKLGDVLGRRTTLLLGIVIFAAGNVLTAVATSLIF 101
Query: 242 PLGVMIFLGVSYSVLASSLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQDK 421
L +G+ + ++ + + P + G AFG+ + + + V GLI +
Sbjct: 102 MLAARFIVGIGTAAISPIVMAYIVTEYPQEETGKAFGLYMLISSGAVVVGPTCGGLIMNA 161
Query: 422 YGFLILEVFFSVWLCITLVLIVLL 493
G+ ++ +W+C+ L ++V L
Sbjct: 162 AGWRVM-----MWVCVALCVVVFL 180
>sp|P37643|YHJE_ECOLI Inner membrane metabolite transport protein yhjE
Length = 440
Score = 48.1 bits (113), Expect = 2e-05
Identities = 40/130 (30%), Positives = 61/130 (46%), Gaps = 8/130 (6%)
Frame = +2
Query: 131 PVMGFLIDLTGRNLFWVLTGTLATLGSHALYAFT----FANPLGVMIFLGVSYSVLASSL 298
PV G L D GR V+ TL L AL+AF NP+ V FL + S++ +
Sbjct: 306 PVAGLLADAFGRRKSMVIITTLIIL--FALFAFNPLLGSGNPILVFAFLLLGLSLMGLTF 363
Query: 299 WPMVALV---IPAHQRGTAFGIMQSVQN-LGLAVVAIIAGLIQDKYGFLILEVFFSVWLC 466
PM AL+ P R T +V + LG +V IA +Q YG + ++ +
Sbjct: 364 GPMGALLPELFPTEVRYTGASFSYNVASILGASVAPYIAAWLQTNYGLGAVGLYLAAMAG 423
Query: 467 ITLVLIVLLY 496
+TL+ ++L +
Sbjct: 424 LTLIALLLTH 433
>sp|P46333|CSBC_BACSU Probable metabolite transport protein csbC
Length = 461
Score = 48.1 bits (113), Expect = 2e-05
Identities = 53/200 (26%), Positives = 85/200 (42%), Gaps = 17/200 (8%)
Frame = +2
Query: 50 LIQKYGLNGADAALITSLLYIVSAACSPVMGFLIDLTGRN--LFW----------VLTGT 193
+ K GL + +AL T + I++ LID GR L W L+G
Sbjct: 268 IFTKAGLGTSASALGTMGIGILNVIMCITAMILIDRVGRKKLLIWGSVGITLSLAALSGV 327
Query: 194 LATLGSHALYAFTFANPLGVMIFLGVSYSVLASSLWPMVALVI-----PAHQRGTAFGIM 358
L TLG A A+ ++FLGV Y V + W V V+ P+ RG A G
Sbjct: 328 LLTLGLSASTAWM------TVVFLGV-YIVFYQATWGPVVWVLMPELFPSKARGAATGFT 380
Query: 359 QSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLYLWDVQNGGKLNESG 538
V + +V+++ L+ G + + FSV +C+ L Y+ G L E
Sbjct: 381 TLVLSAANLIVSLVFPLMLSAMGIAWVFMVFSV-ICL-LSFFFAFYMVPETKGKSLEEIE 438
Query: 539 PSRRKKLKKSEYNVSETMDK 598
S +K+ KK + ++ +++
Sbjct: 439 ASLKKRFKKKKSTQNQVLNE 458
>sp|Q92EE1|LMRB_LISIN Lincomycin resistance protein lmrB
Length = 471
Score = 45.8 bits (107), Expect = 1e-04
Identities = 43/173 (24%), Positives = 79/173 (45%), Gaps = 8/173 (4%)
Frame = +2
Query: 2 AYYVAVFPFVSLSKVF--LIQKYGLNGADAALITSLLYIVSAACSPVMGFLID-LTGRNL 172
A ++ +F +L+ LIQ + ++ A +T+ + P+ G L+ T R L
Sbjct: 23 AGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTRGL 82
Query: 173 FWV-----LTGTLATLGSHALYAFTFANPLGVMIFLGVSYSVLASSLWPMVALVIPAHQR 337
F+ + GTL A + TFA + + V ++L ++ + L+ P H+R
Sbjct: 83 FFTAVSFSIAGTLI-----AALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKR 137
Query: 338 GTAFGIMQSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLY 496
G+A G++ V AV I+GLI + L + W+ + ++I LL+
Sbjct: 138 GSAMGMIGLVIMFAPAVGPTISGLILEN-----LTWNWIFWISLPFLIIALLF 185
>sp|Q8Y9K8|LMRB_LISMO Lincomycin resistance protein lmrB
Length = 471
Score = 45.8 bits (107), Expect = 1e-04
Identities = 43/173 (24%), Positives = 79/173 (45%), Gaps = 8/173 (4%)
Frame = +2
Query: 2 AYYVAVFPFVSLSKVF--LIQKYGLNGADAALITSLLYIVSAACSPVMGFLID-LTGRNL 172
A ++ +F +L+ LIQ + ++ A +T+ + P+ G L+ T R L
Sbjct: 23 AGFIGLFSETALNMALSDLIQVFDISSATVQWLTTGYLLTLGILVPISGLLLQWFTTRGL 82
Query: 173 FWV-----LTGTLATLGSHALYAFTFANPLGVMIFLGVSYSVLASSLWPMVALVIPAHQR 337
F+ + GTL A + TFA + + V ++L ++ + L+ P H+R
Sbjct: 83 FFTAVSFSIAGTLI-----AALSPTFAMLMIGRVVQAVGTALLLPLMFNTILLIFPEHKR 137
Query: 338 GTAFGIMQSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLY 496
G+A G++ V AV I+GLI + L + W+ + ++I LL+
Sbjct: 138 GSAMGMIGLVIMFAPAVGPTISGLILEN-----LTWNWIFWISLPFLIIALLF 185
>sp|P46907|NARK_BACSU Nitrite extrusion protein (Nitrite facilitator)
Length = 395
Score = 45.4 bits (106), Expect = 1e-04
Identities = 50/177 (28%), Positives = 81/177 (45%), Gaps = 13/177 (7%)
Frame = +2
Query: 5 YYVAVFPFVSLSKV---FLIQKYGLNGADAALITSLLYIVSAACSPVMGFLIDLTG--RN 169
Y++ FV+ + FL++ +GLN ADA L T+ VS P GFL D R
Sbjct: 211 YFITFGAFVAFTIYLPNFLVEHFGLNPADAGLRTAGFIAVSTLLRPAGGFLADKMSPLRI 270
Query: 170 LFWVLTGTLATLGSHALYAFTFANPLGVMIFLGVSYSVLASSLWPMVALVIP---AHQRG 340
L +V TG TL L +F+ +G+ F ++ +V + V ++P + Q G
Sbjct: 271 LMFVFTG--LTLSGIIL---SFSPTIGLYTFGSLTVAVCSGIGNGTVFKLVPFYFSKQAG 325
Query: 341 TAFGIMQSVQNLG-----LAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLY 496
A GI+ ++ LG L + ++ Q GF+ L S + VL++ +Y
Sbjct: 326 IANGIVSAMGGLGGFFPPLILASVFQATGQYAIGFMAL----SEVALASFVLVIWMY 378
>sp|P47234|LACY_CITFR Lactose permease (Lactose-proton symport)
Length = 416
Score = 44.3 bits (103), Expect = 3e-04
Identities = 46/188 (24%), Positives = 82/188 (43%), Gaps = 14/188 (7%)
Frame = +2
Query: 14 AVFPFVSLSKVFLIQKYGLNGADAALITSLLYIVSAACSPVMGFLIDLTG--RNLFWVLT 187
A FPF ++L + ++ D +I + + + S P+ G L D G ++L WV+T
Sbjct: 25 AYFPFFP---IWLHEVNHISKGDTGIIFACISLFSLLFQPIFGLLSDKLGLRKHLLWVIT 81
Query: 188 GTLATLGSHALYAFTFANPLGVM-------IFLGVSYSVLASSLWPMVALVIPAHQRGT- 343
G L +Y F + ++ I+LG Y+ A ++ A + A +R
Sbjct: 82 GMLVMFAPFFIYVFGPLLQVNILLGSIVGGIYLGFIYNAGAPAI---EAYIEKASRRSNF 138
Query: 344 AFGIMQSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWL----CITLVLIVLLYLWDVQ 511
FG + +G A+ A IAG++ + F WL + L L++L DV
Sbjct: 139 EFGRARMFGCVGWALCASIAGIM------FTINNQFVFWLGSGCAVILALLLLFSKTDVP 192
Query: 512 NGGKLNES 535
+ K+ ++
Sbjct: 193 SSAKVADA 200
>sp|Q58955|YF60_METJA Putative transporter MJ1560
Length = 386
Score = 43.1 bits (100), Expect = 7e-04
Identities = 32/147 (21%), Positives = 69/147 (46%), Gaps = 1/147 (0%)
Frame = +2
Query: 62 YGLNGADAALITSLLYIVSAACSPVMGFLIDLTGRNLFWVLTGTLATLGSHALY-AFTFA 238
Y + + + +L I+ A G L D G N+ ++ + + G + L + TF
Sbjct: 240 YNITISQVGFMIALTNILMALLQRSFGKLYDKLG-NIMIIIGIFIISFGMYLLSTSTTFL 298
Query: 239 NPLGVMIFLGVSYSVLASSLWPMVALVIPAHQRGTAFGIMQSVQNLGLAVVAIIAGLIQD 418
L + + V S+ +++ + IP H++G A G+ + N+G+ + A+ G + D
Sbjct: 299 TILASLTIIAVGSSISSTATTSLAVKDIPTHRKGEAMGLFTTSINIGMFIGAVSFGFLAD 358
Query: 419 KYGFLILEVFFSVWLCITLVLIVLLYL 499
G + F +++ ++V+ ++ YL
Sbjct: 359 ILGIANMYKFSAIF---SIVVGIISYL 382
>sp|P58120|PMRA_LACLA Multi-drug resistance efflux pump pmrA homolog
Length = 405
Score = 41.6 bits (96), Expect = 0.002
Identities = 40/165 (24%), Positives = 72/165 (43%), Gaps = 5/165 (3%)
Frame = +2
Query: 17 VFPFVSLSKVFLIQKYGLNGADAALITSLLYIVSAACS----PVMGFLIDLTGRNLFWVL 184
V PF+ L IQ G++G + L + L + + A S P+ G L D GR + V
Sbjct: 30 VMPFLPL----YIQGLGVSGGNVELYSGLAFSLPALASGLVAPIWGRLADEHGRKVMMVR 85
Query: 185 TGTLATLGSHAL-YAFTFANPLGVMIFLGVSYSVLASSLWPMVALVIPAHQRGTAFGIMQ 361
+ TL + +A LG+ + +G + +S M+A P + G A G +
Sbjct: 86 ASIVMTLTMGGIAFAPNVWWLLGLRLLMGFFSGYIPNST-AMIASQAPKDKSGYALGTLA 144
Query: 362 SVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLY 496
+ G + + GL+ + +G + VF V + L ++ ++
Sbjct: 145 TAMVSGTLIGPSLGGLLAEWFG--MANVFLIVGALLALATLLTIF 187
>sp|Q57QK4|MDTG_SALCH Multidrug resistance protein mdtG
Length = 404
Score = 40.8 bits (94), Expect = 0.004
Identities = 39/179 (21%), Positives = 73/179 (40%), Gaps = 8/179 (4%)
Frame = +2
Query: 2 AYYVAVFPFVSLSKVFLIQKYGLNGADA-----ALITSLLYIVSAACSPVMGFLIDLTGR 166
A + V PF+ L +++ G+ G A L+ S+ ++ SA SP G L D GR
Sbjct: 26 AAFSLVMPFLPL----YVEQLGVTGHSALNMWSGLVFSITFLFSAIASPFWGGLADRKGR 81
Query: 167 NLFWVLTGTLATLGSHALYAFTFANPLGVMIFLGVSYSVLASSLWPMVALV---IPAHQR 337
+ + + + + A + + L +L + AL+ +P H+
Sbjct: 82 KIMLLRSALGMAI---VMLLMGMAQNIWQFLILRALLGLLGGFILNANALIATQVPRHKS 138
Query: 338 GTAFGIMQSVQNLGLAVVAIIAGLIQDKYGFLILEVFFSVWLCITLVLIVLLYLWDVQN 514
G A G + + G + + GL+ D YG L F + + + +L + + +N
Sbjct: 139 GWALGTLSTGGVSGALLGPLAGGLLADHYG---LRPVFFITASVLFICFLLTFFFIREN 194
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 83,034,059
Number of Sequences: 369166
Number of extensions: 1694836
Number of successful extensions: 4682
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4497
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4667
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6413660220
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)