Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_N15
(864 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding ... 113 8e-25
sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding ... 102 1e-21
sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding ... 100 5e-21
sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding ... 98 3e-20
sp|O94260|G3BP_SCHPO Putative G3BP-like protein 59 2e-08
sp|Q99383|NAB4_YEAST Nuclear polyadenylated RNA-binding pro... 47 9e-05
sp|P48809|RB27C_DROME Heterogeneous nuclear ribonucleoprote... 46 1e-04
sp|Q96EP5|DAZP1_HUMAN DAZ-associated protein 1 (Deleted in ... 45 3e-04
sp|Q9JII5|DAZP1_MOUSE DAZ-associated protein 1 (Deleted in ... 45 3e-04
sp|Q98SJ2|DAZP1_XENLA DAZ-associated protein 1 (Deleted in ... 45 3e-04
>sp|Q13283|G3BP_HUMAN Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 466
Score = 113 bits (282), Expect = 8e-25
Identities = 92/322 (28%), Positives = 149/322 (46%), Gaps = 45/322 (13%)
Frame = +1
Query: 22 ILIQVTGEIANNGCDLRRFMQSFLLVEQD--LGNFYVLNDIFRYQDQTF-----KVEDVE 180
+++QV G ++NN LRRFMQ+F+L + FYV NDIFRYQD+ F + ++
Sbjct: 90 VVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEES 149
Query: 181 EAPVVEHESKNE------DIHGEINSWNELSRNCELNNEQIPQSPQLIEHEEGSEEFINY 342
E V E E + + D G +S + E + E+ P+ E +E ++
Sbjct: 150 EEEVEEPEERQQTPEVVPDDSGTFYDQAVVSNDMEEHLEEPVAEPEPDPEPEPEQEPVS- 208
Query: 343 KIMNEEKDISSEIPVEIDSQQ-------DIGQKMEEMNIKEKSWAAI------------- 462
+I E+ + E D+Q+ DI Q ++E +++ SWA++
Sbjct: 209 EIQEEKPEPVLEETAPEDAQKSSSPAPADIAQTVQE-DLRTFSWASVTSKNLPPSGAVPV 267
Query: 463 -------INPMSSRPSXXXXXXXXXXXXXXXXXXXXINSNGDNMEKRKPR-----FNNGN 606
+ +S+P + +R PR G+
Sbjct: 268 TGIPPHVVKVPASQPRPESKPESQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEQGD 327
Query: 607 VKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNF 786
++ + +PD HQLF+GNLP + + ELKDFF + YG V ++RI N G LPNF
Sbjct: 328 IEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF-QSYGNVVELRI------NSGGKLPNF 380
Query: 787 GFLVFHNHEVVEEILKNKPIYY 852
GF+VF + E V+++L N+PI +
Sbjct: 381 GFVVFDDSEPVQKVLSNRPIMF 402
>sp|P97855|G3BP_MOUSE Ras-GTPase-activating protein binding protein 1 (GAP SH3-domain
binding protein 1) (G3BP-1) (DNA helicase VIII)
(HDH-VIII)
Length = 465
Score = 102 bits (255), Expect = 1e-21
Identities = 89/323 (27%), Positives = 147/323 (45%), Gaps = 46/323 (14%)
Frame = +1
Query: 22 ILIQVTGEIANNGCDLRRFMQSFLLVEQD--LGNFYVLNDIFRYQDQTF----------K 165
+++QV G ++NN LRRFMQ+F+L + FYV NDIFRYQD+ F
Sbjct: 90 VVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGGFVTEPQEES 149
Query: 166 VEDVEEAPVVEHESKNEDIHGEINSWNE--LSRNCELNNEQIPQSPQLIEHEEGSEEFIN 339
E+VEE E + E + + ++ + +S + E + E+ P+ E E ++
Sbjct: 150 EEEVEEPE--ERQQTPEVVPDDSGTFYDQTVSNDLEEHLEEPVVEPEPEPEPEPEPEPVS 207
Query: 340 YKIMNEEKDISSEIPVEIDSQQ-------DIGQKMEEMNIKEKSWAAIIN---------P 471
I ++ + + E D Q+ D+ E++ + SWA++ + P
Sbjct: 208 -DIQEDKPEAALEEAAPDDVQKSTSPAPADVAPAQEDL--RTFSWASVTSKNLPPSGAVP 264
Query: 472 MSSRPSXXXXXXXXXXXXXXXXXXXXINSNGDNMEK-RKPRFN---------------NG 603
++ P ++ R+ R N G
Sbjct: 265 VTGTPPHVVKVPASQPRPESKPDSQIPPQRPQRDQRVREQRINIPPQRGPRPIREAGEPG 324
Query: 604 NVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPN 783
+V+ + +PD HQLF+GNLP + + ELKDFF + +G V ++RI N G LPN
Sbjct: 325 DVEPRRMVRHPDSHQLFIGNLPHEVDKSELKDFF-QNFGNVVELRI------NSGGKLPN 377
Query: 784 FGFLVFHNHEVVEEILKNKPIYY 852
FGF+VF + E V+++L N+PI +
Sbjct: 378 FGFVVFDDSEPVQKVLSNRPIMF 400
>sp|Q9UN86|G3B2_HUMAN Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 100 bits (249), Expect = 5e-21
Identities = 87/318 (27%), Positives = 142/318 (44%), Gaps = 41/318 (12%)
Frame = +1
Query: 22 ILIQVTGEIANNGCDLRRFMQSFLLVEQDL--GNFYVLNDIFRYQDQTF---------KV 168
+++QV G ++N+G R+FMQ+F+L + FYV ND+FRY+D+ F +
Sbjct: 90 VVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEES 149
Query: 169 EDVEEAPVVEHESKNEDIHGEINSW----NELSRNCELNNEQIPQSPQLIEHEEGSEEFI 336
ED E E + E + NS + ++ E E+ P+ E E +
Sbjct: 150 EDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEEL 209
Query: 337 NYKIMNEEKDISSEIPVEIDSQQDIGQKMEEMNIKEK-----SWAAIIN---PMSSR--P 486
++ EEK++ E++ + E +++ ++ SWA++ + P S
Sbjct: 210 KPQV--EEKNLE-----ELEEKSTTPPPAEPVSLPQEPPKAFSWASVTSKNLPPSGTVSS 262
Query: 487 SXXXXXXXXXXXXXXXXXXXXINSNGDNMEKRKPRFNNG----------------NVKTP 618
S + S + +++PR G +
Sbjct: 263 SGIPSHVKAPVSQPRVEAKPEVQSQPPRVREQRpreRPGFPPRGPRPGRGDMEQNDSDNR 322
Query: 619 STLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLV 798
+ YPD HQLFVGNLP ++ E+ELK+FF +G V ++RI T G LPNFGF+V
Sbjct: 323 RIIRYPDSHQLFVGNLPHDIDENELKEFFM-SFGNVVELRI---NTKGVGGKLPNFGFVV 378
Query: 799 FHNHEVVEEILKNKPIYY 852
F + E V+ IL KPI +
Sbjct: 379 FDDSEPVQRILIAKPIMF 396
>sp|P97379|G3B2_MOUSE Ras-GTPase-activating protein binding protein 2 (GAP SH3-domain
binding protein 2) (G3BP-2)
Length = 482
Score = 97.8 bits (242), Expect = 3e-20
Identities = 85/318 (26%), Positives = 140/318 (44%), Gaps = 41/318 (12%)
Frame = +1
Query: 22 ILIQVTGEIANNGCDLRRFMQSFLLVEQDL--GNFYVLNDIFRYQDQTF----------- 162
+++QV G ++N+G R+FMQ+F+L + FYV ND+FRY+D+ F
Sbjct: 90 VVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGDSEPELDEES 149
Query: 163 --KVEDVEEAPVVEHESKNEDIHGEINSWNELSRNCELNNEQIPQSPQLIEHEEGSEEFI 336
+VE+ +E E E+ + + ++ E E+ P+ E E +
Sbjct: 150 EDEVEEEQEDRQPSPEPVQENANSAYYDAHPVTNGIEEPLEESSHEPEPEPESETKTEEL 209
Query: 337 NYKIMNEEKDISSEIPVEIDSQQDIGQKMEEMNIKEK-----SWAAIIN---PMSSR--P 486
++ EEK + E++ + E ++ ++ SWA++ + P S
Sbjct: 210 KPQV--EEKHLE-----ELEEKSATPPPAEPASLPQEPPKAFSWASVTSKNLPPSGTVSS 262
Query: 487 SXXXXXXXXXXXXXXXXXXXXINSNGDNMEKRKPRFNNG----------------NVKTP 618
S + S + +++PR G +
Sbjct: 263 SGIPPHVKAPVSQPRVDAKPEVQSQPPRVREQRpreRPGFPPRGPRPGRGDMEQNDSDNR 322
Query: 619 STLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLV 798
+ YPD HQLFVGNLP ++ E+ELK+FF +G V ++RI T G LPNFGF+V
Sbjct: 323 RIIRYPDSHQLFVGNLPHDIDENELKEFFM-SFGNVVELRI---NTKGVGGKLPNFGFVV 378
Query: 799 FHNHEVVEEILKNKPIYY 852
F + E V+ IL KPI +
Sbjct: 379 FDDSEPVQRILIAKPIMF 396
>sp|O94260|G3BP_SCHPO Putative G3BP-like protein
Length = 434
Score = 58.5 bits (140), Expect = 2e-08
Identities = 78/309 (25%), Positives = 121/309 (39%), Gaps = 29/309 (9%)
Frame = +1
Query: 7 SHGKSILIQVTGEIANNGCDLRRFMQSFLLVEQDLGNFYVLNDIFRYQDQTFKVEDVEEA 186
S I+IQV GE++N G R+F Q+F L EQ G ++VLNDIFR+ + VE+ EE+
Sbjct: 89 SSNGGIVIQVLGEMSNKGKLSRKFAQTFFLAEQPNG-YFVLNDIFRFLRE--DVEEEEES 145
Query: 187 P-VVEHESK---NEDIHGEINSWNELSRNCELNNEQIPQSPQ-------LIEHEEGSEEF 333
P VE E K +E + S L E + Q P + + LI +E S
Sbjct: 146 PDAVEKEKKDVASEPYVNGVQSQEHLPSAKEEGHYQDPAATENNFATAALISNETDSLNQ 205
Query: 334 INYKIMNE------EKDISSEIPVEIDSQQD------------IGQKMEEMNIKEKSWAA 459
+ E E + S + + + QD IG + KSWA
Sbjct: 206 ATLAVPEEPVIQVTEASVPSFVSQQENQLQDEALTSNSKNADAIGASDANVATAPKSWAD 265
Query: 460 IINPMSSRPSXXXXXXXXXXXXXXXXXXXXINSNGDNMEKRKPRFNNGNVKTPSTLNYPD 639
+I + P +N+ D ++ + N
Sbjct: 266 LI--ARNHPDVKSQASVSSTASTTGQTVKGVNA--DQTQQPTAPYTQSNELL-------- 313
Query: 640 EHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVV 819
E +FV N+P ++ LK S +GPVK + + + + ++ F NHE V
Sbjct: 314 ETSVFVKNIPPETSDVSLKSAMS-IFGPVKAIEFARRKGT---------AYVDFVNHECV 363
Query: 820 EEILKNKPI 846
+ L K +
Sbjct: 364 QLALNKKTL 372
>sp|Q99383|NAB4_YEAST Nuclear polyadenylated RNA-binding protein 4
Length = 534
Score = 46.6 bits (109), Expect = 9e-05
Identities = 24/67 (35%), Positives = 39/67 (58%)
Frame = +1
Query: 646 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
++F+G L + TED L+++F KYG V D++I K + + FGFL F V+E
Sbjct: 160 KMFIGGLNWDTTEDNLREYFG-KYGTVTDLKIMKDPATGRSR---GFGFLSFEKPSSVDE 215
Query: 826 ILKNKPI 846
++K + I
Sbjct: 216 VVKTQHI 222
Score = 35.0 bits (79), Expect = 0.27
Identities = 15/65 (23%), Positives = 38/65 (58%)
Frame = +1
Query: 646 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
++FVG + ++ E ++FFS+ +G + D ++ + + + + FGF+ + + + V+
Sbjct: 244 KIFVGGIGPDVRPKEFEEFFSQ-WGTIIDAQLMLDKDTGQSR---GFGFVTYDSADAVDR 299
Query: 826 ILKNK 840
+ +NK
Sbjct: 300 VCQNK 304
>sp|P48809|RB27C_DROME Heterogeneous nuclear ribonucleoprotein 27C (Hrb27-C) (hnRNP 48)
(HRP48.1)
Length = 421
Score = 46.2 bits (108), Expect = 1e-04
Identities = 29/80 (36%), Positives = 43/80 (53%), Gaps = 3/80 (3%)
Frame = +1
Query: 616 PSTLNYPDE---HQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNF 786
P TL P + +++F+G LP N+TE +L+ FF+ +YG V +V I + E K F
Sbjct: 84 PRTLQKPKKGGGYKVFLGGLPSNVTETDLRTFFN-RYGKVTEVVIMYDQ---EKKKSRGF 139
Query: 787 GFLVFHNHEVVEEILKNKPI 846
GFL F VE + + I
Sbjct: 140 GFLSFEEESSVEHVTNERYI 159
Score = 35.0 bits (79), Expect = 0.27
Identities = 21/66 (31%), Positives = 34/66 (51%)
Frame = +1
Query: 646 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
+LFVG L T++ L +F ++G + D + K+ NE FGF+ F + V
Sbjct: 8 KLFVGGLSWETTQENLSRYFC-RFGDIIDCVVMKN---NESGRSRGFGFVTFADPTNVNH 63
Query: 826 ILKNKP 843
+L+N P
Sbjct: 64 VLQNGP 69
>sp|Q96EP5|DAZP1_HUMAN DAZ-associated protein 1 (Deleted in azoospermia-associated protein
1)
Length = 407
Score = 45.1 bits (105), Expect = 3e-04
Identities = 24/66 (36%), Positives = 40/66 (60%)
Frame = +1
Query: 646 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
+LFVG L + T++ L+ +FS+ YG V D I K +T+N+ + FGF+ F + V
Sbjct: 11 KLFVGGLDWSTTQETLRSYFSQ-YGEVVDCVIMKDKTTNQSR---GFGFVKFKDPNCVGT 66
Query: 826 ILKNKP 843
+L ++P
Sbjct: 67 VLASRP 72
Score = 37.7 bits (86), Expect = 0.042
Identities = 24/85 (28%), Positives = 44/85 (51%)
Frame = +1
Query: 571 MEKRKPRFNNGNVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKS 750
M+ + R G K P + N +++FVG +P N E EL+++F +K+G V +V +
Sbjct: 90 MQPERTRPKEGWQKGPRSDN-SKSNKIFVGGIPHNCGETELREYF-KKFGVVTEVVMIYD 147
Query: 751 RTSNEGKPLPNFGFLVFHNHEVVEE 825
+ FGF+ F + + V++
Sbjct: 148 AEKQRPR---GFGFITFEDEQSVDQ 169
>sp|Q9JII5|DAZP1_MOUSE DAZ-associated protein 1 (Deleted in azoospermia-associated protein
1)
Length = 406
Score = 45.1 bits (105), Expect = 3e-04
Identities = 24/66 (36%), Positives = 40/66 (60%)
Frame = +1
Query: 646 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
+LFVG L + T++ L+ +FS+ YG V D I K +T+N+ + FGF+ F + V
Sbjct: 11 KLFVGGLDWSTTQETLRSYFSQ-YGEVVDCVIMKDKTTNQSR---GFGFVKFKDPNCVGT 66
Query: 826 ILKNKP 843
+L ++P
Sbjct: 67 VLASRP 72
Score = 36.6 bits (83), Expect = 0.093
Identities = 23/85 (27%), Positives = 44/85 (51%)
Frame = +1
Query: 571 MEKRKPRFNNGNVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKS 750
M+ + R G K P + + +++FVG +P N E EL+++F +K+G V +V +
Sbjct: 90 MQPERTRPKEGWQKGPRS-DSSKSNKIFVGGIPHNCGETELREYF-KKFGVVTEVVMIYD 147
Query: 751 RTSNEGKPLPNFGFLVFHNHEVVEE 825
+ FGF+ F + + V++
Sbjct: 148 AEKQRPR---GFGFITFEDEQSVDQ 169
>sp|Q98SJ2|DAZP1_XENLA DAZ-associated protein 1 (Deleted in azoospermia-associated protein
1) (Proline-rich Vg1 mRNA-binding protein)
Length = 360
Score = 45.1 bits (105), Expect = 3e-04
Identities = 24/66 (36%), Positives = 40/66 (60%)
Frame = +1
Query: 646 QLFVGNLPQNMTEDELKDFFSEKYGPVKDVRIQKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
+LFVG L + T++ L+ +FS+ YG V D I K +T+N+ + FGF+ F + V
Sbjct: 11 KLFVGGLDWSTTQETLRSYFSQ-YGEVVDCVIMKDKTTNQSR---GFGFVKFKDPNCVGT 66
Query: 826 ILKNKP 843
+L ++P
Sbjct: 67 VLASRP 72
Score = 38.5 bits (88), Expect = 0.024
Identities = 26/88 (29%), Positives = 45/88 (51%)
Frame = +1
Query: 562 GDNMEKRKPRFNNGNVKTPSTLNYPDEHQLFVGNLPQNMTEDELKDFFSEKYGPVKDVRI 741
G E+ +PR K P T N +++FVG +P N E ELK++F+ ++G V +V +
Sbjct: 89 GMQPERSRPR-EGWQQKEPRTEN-SRSNKIFVGGIPHNCGETELKEYFN-RFGVVTEVVM 145
Query: 742 QKSRTSNEGKPLPNFGFLVFHNHEVVEE 825
+ FGF+ F + + V++
Sbjct: 146 IYDAEKQRPR---GFGFITFEDEQSVDQ 170
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.312 0.132 0.378
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 85,744,260
Number of Sequences: 369166
Number of extensions: 1580901
Number of successful extensions: 4828
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4567
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4798
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8582957970
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.8 bits)