Planarian EST Database


Dr_sW_027_N07

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_027_N07
         (371 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O14880|MGST3_HUMAN  Microsomal glutathione S-transferase ...    66   3e-11
sp|Q9CPU4|MGST3_MOUSE  Microsomal glutathione S-transferase ...    65   5e-11
sp|P73795|Y1147_SYNY3  Hypothetical protein sll1147                39   0.003
sp|P61682|MURC_RHOPA  UDP-N-acetylmuramate--L-alanine ligase...    28   6.6  
sp|P30355|FLAP_MOUSE  5-lipoxygenase-activating protein (FLA...    28   8.6  
sp|P20291|FLAP_RAT  5-lipoxygenase-activating protein (FLAP)...    28   8.6  
>sp|O14880|MGST3_HUMAN Microsomal glutathione S-transferase 3 (Microsomal GST-3)
           (Microsomal GST-III)
          Length = 152

 Score = 65.9 bits (159), Expect = 3e-11
 Identities = 34/74 (45%), Positives = 46/74 (62%), Gaps = 3/74 (4%)
 Frame = +1

Query: 1   MYSKDSKT---FNCIQRVHQNYVETVPFFWLVLFLGGFQNPKLAAIFGGAFIVSRIIYSL 171
           MYS D +    FNCIQR HQN +E  P F   L +GG  +P++A+  G A+IV R++Y+ 
Sbjct: 43  MYSTDPENGHIFNCIQRAHQNTLEVYPPFLFFLAVGGVYHPRIASGLGLAWIVGRVLYAY 102

Query: 172 GYSSGIADKRRPGA 213
           GY +G   KR  GA
Sbjct: 103 GYYTGEPSKRSRGA 116
>sp|Q9CPU4|MGST3_MOUSE Microsomal glutathione S-transferase 3 (Microsomal GST-3)
           (Microsomal GST-III)
          Length = 153

 Score = 65.1 bits (157), Expect = 5e-11
 Identities = 33/74 (44%), Positives = 46/74 (62%), Gaps = 3/74 (4%)
 Frame = +1

Query: 1   MYSKDSKT---FNCIQRVHQNYVETVPFFWLVLFLGGFQNPKLAAIFGGAFIVSRIIYSL 171
           MYS D +    FNCIQR HQN +E  P F   L +GG  +P++A+  G A+I+ R++Y+ 
Sbjct: 43  MYSTDPENGHMFNCIQRAHQNTLEVYPPFLFFLTVGGVYHPRIASGLGLAWIIGRVLYAY 102

Query: 172 GYSSGIADKRRPGA 213
           GY +G   KR  GA
Sbjct: 103 GYYTGDPSKRYRGA 116
>sp|P73795|Y1147_SYNY3 Hypothetical protein sll1147
          Length = 137

 Score = 39.3 bits (90), Expect = 0.003
 Identities = 23/66 (34%), Positives = 39/66 (59%), Gaps = 1/66 (1%)
 Frame = +1

Query: 16  SKTFNCIQRVHQNYVETVPFFWLVLFLGG-FQNPKLAAIFGGAFIVSRIIYSLGYSSGIA 192
           ++ F  + RV  N +E + FF   L+L   +++P +AAI G  +++ RI+Y+ GY    A
Sbjct: 44  NEDFERVLRVQYNTLEQLAFFLPGLWLFAIYRDPTIAAILGAVWLLGRILYAWGYYQA-A 102

Query: 193 DKRRPG 210
           +KR  G
Sbjct: 103 EKRMVG 108
>sp|P61682|MURC_RHOPA UDP-N-acetylmuramate--L-alanine ligase
           (UDP-N-acetylmuramoyl-L-alanine synthetase)
          Length = 467

 Score = 28.1 bits (61), Expect = 6.6
 Identities = 10/20 (50%), Positives = 15/20 (75%)
 Frame = +1

Query: 19  KTFNCIQRVHQNYVETVPFF 78
           KTF+ +Q   +N+VE VPF+
Sbjct: 192 KTFDAVQDAFRNFVENVPFY 211
>sp|P30355|FLAP_MOUSE 5-lipoxygenase-activating protein (FLAP) (MK-886-binding protein)
          Length = 161

 Score = 27.7 bits (60), Expect = 8.6
 Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = +1

Query: 25  FNCIQRVHQNYVETVPFFWLVLFLGGFQNPKLAAIFGG-AFIVSRIIYSLGY 177
           F  +   +QN V+  P F +VL+  G    ++ A F G  ++  R  Y +GY
Sbjct: 50  FERVYTANQNCVDAYPTFLVVLWTAGLLCSQVPAAFAGLMYLFVRQKYFVGY 101
>sp|P20291|FLAP_RAT 5-lipoxygenase-activating protein (FLAP) (MK-886-binding protein)
          Length = 161

 Score = 27.7 bits (60), Expect = 8.6
 Identities = 16/52 (30%), Positives = 26/52 (50%), Gaps = 1/52 (1%)
 Frame = +1

Query: 25  FNCIQRVHQNYVETVPFFWLVLFLGGFQNPKLAAIFGG-AFIVSRIIYSLGY 177
           F  +   +QN V+  P F +VL+  G    ++ A F G  ++  R  Y +GY
Sbjct: 50  FERVYTANQNCVDAYPTFLVVLWTAGLLCSQVPAAFAGLMYLFVRQKYFVGY 101
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 39,592,954
Number of Sequences: 369166
Number of extensions: 684531
Number of successful extensions: 1249
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 1240
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1248
length of database: 68,354,980
effective HSP length: 90
effective length of database: 51,728,830
effective search space used: 1707051390
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)