Planarian EST Database


Dr_sW_027_M16

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_027_M16
         (471 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q9CRB6|CG38_MOUSE  Protein CGI-38 homolog                       76   3e-14
sp|Q9BW30|CG38_HUMAN  Protein CGI-38                               76   3e-14
sp|P59282|P25B_HUMAN  Protein p25-beta                             74   1e-13
sp|Q7TQD2|P25A_MOUSE  Tubulin polymerization-promoting prote...    72   5e-13
sp|O94811|P25A_HUMAN  Tubulin polymerization-promoting prote...    71   1e-12
sp|Q27957|P25A_BOVIN  Tubulin polymerization-promoting prote...    70   3e-12
sp|P91127|YBYK_CAEEL  Hypothetical protein C32E8.3 in chromo...    65   8e-11
sp|Q9VV43|Y4893_DROME  Hypothetical protein CG4893                 64   1e-10
sp|Q4L5U2|TRMD_STAHJ  tRNA (Guanine-N(1)-)-methyltransferase...    33   0.43 
sp|P16342|R1AB_CVMA5  Replicase polyprotein 1ab (pp1ab) (ORF...    32   0.95 
>sp|Q9CRB6|CG38_MOUSE Protein CGI-38 homolog
          Length = 176

 Score = 76.3 bits (186), Expect = 3e-14
 Identities = 51/112 (45%), Positives = 61/112 (54%), Gaps = 20/112 (17%)
 Frame = +2

Query: 53  SARNLAYED---AVQELKTK-------------ICQ----GSPAGHGTTGVSADAATARL 172
           SAR + YE+   A++EL TK             ICQ      PA  G T      A  RL
Sbjct: 66  SARVINYEEFKKALEELATKRFKGKSKEEAFDAICQLIAGKEPANIGVTKAKTGGAVDRL 125

Query: 173 TDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 328
           TD   YTG+HKERFD E+GKGKGI GR+ +  ++   GYV  YK AGTYD K
Sbjct: 126 TDTSKYTGSHKERFD-ESGKGKGIAGRQDILDDS---GYVSAYKNAGTYDAK 173
>sp|Q9BW30|CG38_HUMAN Protein CGI-38
          Length = 176

 Score = 76.3 bits (186), Expect = 3e-14
 Identities = 51/112 (45%), Positives = 61/112 (54%), Gaps = 20/112 (17%)
 Frame = +2

Query: 53  SARNLAYED---AVQELKTK-------------ICQ----GSPAGHGTTGVSADAATARL 172
           SAR + YE+   A++EL TK             ICQ      PA  G T      A  RL
Sbjct: 66  SARVINYEEFKKALEELATKRFKGKSKEEAFDAICQLVAGKEPANVGVTKAKTGGAVDRL 125

Query: 173 TDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 328
           TD   YTG+HKERFD E+GKGKGI GR+ +  ++   GYV  YK AGTYD K
Sbjct: 126 TDTSRYTGSHKERFD-ESGKGKGIAGRQDILDDS---GYVSAYKNAGTYDAK 173
>sp|P59282|P25B_HUMAN Protein p25-beta
          Length = 170

 Score = 74.3 bits (181), Expect = 1e-13
 Identities = 40/70 (57%), Positives = 44/70 (62%)
 Frame = +2

Query: 119 PAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGG 298
           PA  G T  +   A  RLTD   YTG HKE FD E+GKGKGI GRE +       GYV G
Sbjct: 103 PATTGATKATTVGAVDRLTDTSKYTGTHKELFD-ESGKGKGIAGREEMTDNT---GYVSG 158

Query: 299 YKGAGTYDKK 328
           YKG+GTYDKK
Sbjct: 159 YKGSGTYDKK 168
>sp|Q7TQD2|P25A_MOUSE Tubulin polymerization-promoting protein (TPPP)
          Length = 218

 Score = 72.4 bits (176), Expect = 5e-13
 Identities = 45/106 (42%), Positives = 61/106 (57%)
 Frame = +2

Query: 11  FVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGY 190
           F +F E     A    ++ + E+AV+E+   I   +P   G T   +    +RLTD   +
Sbjct: 112 FEQFQEALEELAKKRFKDKSSEEAVREVHRLIEGRAPVISGVTKAVSSPTVSRLTDTSKF 171

Query: 191 TGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 328
           TG+HKERFD ++GKGKG  GR  L  E+   GYV GYK AGTYD+K
Sbjct: 172 TGSHKERFD-QSGKGKGKAGRVDLVDES---GYVPGYKHAGTYDQK 213
>sp|O94811|P25A_HUMAN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha) (p24) (p25)
          Length = 219

 Score = 71.2 bits (173), Expect = 1e-12
 Identities = 45/106 (42%), Positives = 60/106 (56%)
 Frame = +2

Query: 11  FVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGY 190
           F +F E     A    ++ + E+AV+E+   I   +P   G T   +    +RLTD   +
Sbjct: 113 FEQFQEALEELAKKRFKDKSSEEAVREVHRLIEGKAPIISGVTKAISSPTVSRLTDTTKF 172

Query: 191 TGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 328
           TG+HKERFD  +GKGKG  GR  L  E+   GYV GYK AGTYD+K
Sbjct: 173 TGSHKERFD-PSGKGKGKAGRVDLVDES---GYVSGYKHAGTYDQK 214
>sp|Q27957|P25A_BOVIN Tubulin polymerization-promoting protein (TPPP) (25 kDa
           brain-specific protein) (p25-alpha)
          Length = 218

 Score = 69.7 bits (169), Expect = 3e-12
 Identities = 44/106 (41%), Positives = 60/106 (56%)
 Frame = +2

Query: 11  FVRFVEGPMAEAYMSARNLAYEDAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGY 190
           F +F E     A    ++ + E+AV+E+   I   +P   G T   +    +RLTD   +
Sbjct: 112 FEQFKEALEELAKKRFKDKSAEEAVREVHKLIEGKAPIISGVTKAISSPTVSRLTDTSKF 171

Query: 191 TGAHKERFDAETGKGKGIDGREYLAGENAQQGYVGGYKGAGTYDKK 328
           TG+HKERFD  +G+GKG  GR  L  E+   GYV GYK AGTYD+K
Sbjct: 172 TGSHKERFD-PSGRGKGRAGRVDLVDES---GYVPGYKHAGTYDQK 213
>sp|P91127|YBYK_CAEEL Hypothetical protein C32E8.3 in chromosome I
          Length = 180

 Score = 65.1 bits (157), Expect = 8e-11
 Identities = 40/83 (48%), Positives = 47/83 (56%)
 Frame = +2

Query: 77  DAVQELKTKICQGSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGRE 256
           DA+ E   K+   +P+  G    +A    +RLTD   YTGAHKERFDAE GKGKG  GR 
Sbjct: 100 DAITEKLAKL--EAPSVGGAAKANAAGVYSRLTDHTKYTGAHKERFDAE-GKGKGKSGR- 155

Query: 257 YLAGENAQQGYVGGYKGAGTYDK 325
             A      GYVG YK   +YDK
Sbjct: 156 --ADTTENTGYVGAYKNKDSYDK 176
>sp|Q9VV43|Y4893_DROME Hypothetical protein CG4893
          Length = 192

 Score = 64.3 bits (155), Expect = 1e-10
 Identities = 38/81 (46%), Positives = 48/81 (59%), Gaps = 1/81 (1%)
 Frame = +2

Query: 83  VQELKTKICQ-GSPAGHGTTGVSADAATARLTDVKGYTGAHKERFDAETGKGKGIDGREY 259
           + E+K K+   G+P     +   A AA  RLTD   YTG+HKERFDA +GKGKGI GR  
Sbjct: 113 LSEIKQKLASCGAPGVVSVSAGKAAAAVDRLTDTSKYTGSHKERFDA-SGKGKGIAGRRN 171

Query: 260 LAGENAQQGYVGGYKGAGTYD 322
           +   +   GYV GY+   TYD
Sbjct: 172 VVDGS---GYVSGYQHKDTYD 189
>sp|Q4L5U2|TRMD_STAHJ tRNA (Guanine-N(1)-)-methyltransferase (M1G-methyltransferase)
           (tRNA [GM37] methyltransferase)
          Length = 245

 Score = 32.7 bits (73), Expect = 0.43
 Identities = 21/48 (43%), Positives = 25/48 (52%), Gaps = 1/48 (2%)
 Frame = -2

Query: 179 HLSNEQLPHQQKL-RLYHGRQDYLDRF*FSILVLRLHKPNFLHSYKLQ 39
           H + EQ  H+QKL R YH R D LDR+      L      F+ SYK Q
Sbjct: 199 HANIEQWRHEQKLIRTYHKRPDLLDRY-----PLTEDDEKFIESYKKQ 241
>sp|P16342|R1AB_CVMA5 Replicase polyprotein 1ab (pp1ab) (ORF1ab polyprotein) [Includes:
            Replicase polyprotein 1a (pp1a) (ORF1a)] [Contains: p28;
            p65; p210 (Papain-like proteinases 1/2)
            (PL1-PRO/PL2-PRO); Peptide HD2 (p44); 3C-like proteinase
            (3CL-PRO) (3CLp) (M-PRO) (p27); Unknown protein 1; p10;
            p22; p12; Growth factor-like peptide (GFL) (p15);
            RNA-directed RNA polymerase (RdRp) (Pol) (p100); Helicase
            (Hel) (p67); Unknown protein 2; p35; Unknown protein 3]
          Length = 7176

 Score = 31.6 bits (70), Expect = 0.95
 Identities = 13/45 (28%), Positives = 25/45 (55%)
 Frame = -3

Query: 160  CRISRNSGCTMAGRTTLTDFSFQFLYCVFISQISCTHISFSHWSF 26
            CR++ +  C M+GR +LT  S+Q   C  +  ++  + +   +SF
Sbjct: 3392 CRVTSSDFCVMSGRMSLTVMSYQMQGCQLVLTVTLQNPNTPKYSF 3436
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 48,470,345
Number of Sequences: 369166
Number of extensions: 890388
Number of successful extensions: 3127
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3018
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 3112
length of database: 68,354,980
effective HSP length: 101
effective length of database: 49,696,745
effective search space used: 2733320975
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)