Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_027_L20 (136 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P81439|EQST_ACTEQ Equistatin 31 1.0 sp|P01267|THYG_BOVIN Thyroglobulin precursor 29 2.9 sp|O08710|THYG_MOUSE Thyroglobulin precursor 29 2.9
>sp|P81439|EQST_ACTEQ Equistatin Length = 199 Score = 30.8 bits (68), Expect = 1.0 Identities = 13/28 (46%), Positives = 19/28 (67%), Gaps = 2/28 (7%) Frame = +2 Query: 2 YEKAQCLGSV--CFCADENGKQIGSTTV 79 +E+ QC GS C+C DE+GK+I T + Sbjct: 30 FEEKQCWGSTGYCWCVDEDGKEILGTKI 57
>sp|P01267|THYG_BOVIN Thyroglobulin precursor Length = 2769 Score = 29.3 bits (64), Expect = 2.9 Identities = 9/26 (34%), Positives = 15/26 (57%) Frame = +2 Query: 2 YEKAQCLGSVCFCADENGKQIGSTTV 79 YE+ QC C+C D G+++ + V Sbjct: 625 YEEVQCFAGDCWCVDAQGRELAGSRV 650
>sp|O08710|THYG_MOUSE Thyroglobulin precursor Length = 2766 Score = 29.3 bits (64), Expect = 2.9 Identities = 10/26 (38%), Positives = 14/26 (53%) Frame = +2 Query: 2 YEKAQCLGSVCFCADENGKQIGSTTV 79 YE QC C+C D GK++ + V Sbjct: 626 YEDIQCYAGECWCVDSRGKELDGSRV 651
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,345,133 Number of Sequences: 369166 Number of extensions: 140443 Number of successful extensions: 383 Number of sequences better than 10.0: 3 Number of HSP's better than 10.0 without gapping: 376 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 383 length of database: 68,354,980 effective HSP length: 18 effective length of database: 65,029,750 effective search space used: 1690773500 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)