Planarian EST Database


Dr_sW_027_L05

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_027_L05
         (603 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|Q6PD26|PIGS_MOUSE  GPI transamidase component PIG-S (Phos...    56   6e-08
sp|Q96S52|PIGS_HUMAN  GPI transamidase component PIG-S (Phos...    55   1e-07
sp|Q5XI31|PIGS_RAT  GPI transamidase component PIG-S (Phosph...    55   1e-07
sp|Q10351|PIGS_SCHPO  GPI transamidase component PIG-S homolog     37   0.029
sp|Q51791|PHZE_PSEFL  Anthranilate synthase, phenazine speci...    35   0.11 
sp|Q51519|PHZB_PSECL  Anthranilate synthase, phenazine speci...    34   0.25 
sp|Q9JXK5|PUR4_NEIMB  Phosphoribosylformylglycinamidine synt...    33   0.43 
sp|Q9F6X6|BCHN_CHLAU  Light-independent protochlorophyllide ...    33   0.56 
sp|P45737|CATA_BACFR  Catalase                                     31   2.1  
sp|Q53RB0|LOX4_ORYSA  Probable lipoxygenase 4                      30   3.6  
>sp|Q6PD26|PIGS_MOUSE GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
          Length = 555

 Score = 56.2 bits (134), Expect = 6e-08
 Identities = 43/143 (30%), Positives = 70/143 (48%), Gaps = 8/143 (5%)
 Frame = +3

Query: 126 VRTSAKYDWTFSLIVPHPKGGDSTEWDFPRIEHLYLEPLLRKLAPFISVKVSSQIIYGVR 305
           +++S  Y+ TFSL+ P PK  D   WD       +++P L +L+   +  V SQI+Y   
Sbjct: 221 LKSSLGYEITFSLLNPDPKSHD-VHWDIEGAVQRFVQPFLNRLSVAGNFSVDSQILYYAM 279

Query: 306 LERIWPSKYRDHTNRFYSKEDLTHVVNGIETYLGSNTG-----LHFVGYIASSSEAEGGI 470
           L  + P      ++   +   L HV+N +E+ LGS+       LHF+ Y+   + +   I
Sbjct: 280 L-GVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLHFLLYVPELAHSPLYI 338

Query: 471 QLHTEG---NTDAFAVPRWAACL 530
           Q   +G    T+AF  PRW   +
Sbjct: 339 Q-DKDGAPVATNAFHSPRWGGIM 360
>sp|Q96S52|PIGS_HUMAN GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
          Length = 555

 Score = 55.1 bits (131), Expect = 1e-07
 Identities = 43/143 (30%), Positives = 68/143 (47%), Gaps = 8/143 (5%)
 Frame = +3

Query: 126 VRTSAKYDWTFSLIVPHPKGGDSTEWDFPRIEHLYLEPLLRKLAPFISVKVSSQIIYGVR 305
           +++S  Y+ TFSL+ P PK  D   WD       Y++P L  L    +  V SQI+Y   
Sbjct: 221 LKSSLGYEITFSLLNPDPKSHD-VYWDIEGAVRRYVQPFLNALGAAGNFSVDSQILYYAM 279

Query: 306 LERIWPSKYRDHTNRFYSKEDLTHVVNGIETYLGSNTG-----LHFVGYIASSSEAEGGI 470
           L  + P      ++ +     L HV+N +E+ LGS+       L+F+ Y+   + +   I
Sbjct: 280 L-GVNPRFDSASSSYYLDMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYI 338

Query: 471 QLHTEG---NTDAFAVPRWAACL 530
           Q   +G    T+AF  PRW   +
Sbjct: 339 Q-DKDGAPVATNAFHSPRWGGIM 360
>sp|Q5XI31|PIGS_RAT GPI transamidase component PIG-S (Phosphatidylinositol-glycan
           biosynthesis, class S protein)
          Length = 555

 Score = 55.1 bits (131), Expect = 1e-07
 Identities = 43/143 (30%), Positives = 70/143 (48%), Gaps = 8/143 (5%)
 Frame = +3

Query: 126 VRTSAKYDWTFSLIVPHPKGGDSTEWDFPRIEHLYLEPLLRKLAPFISVKVSSQIIYGVR 305
           +++S  Y+ TFSL+ P PK  D   WD       Y++P L +L+   +  V SQI+Y   
Sbjct: 221 LKSSLGYEITFSLLNPDPKSHD-VHWDIEEGVQRYVQPFLNRLSAAGNFSVDSQILYYAM 279

Query: 306 LERIWPSKYRDHTNRFYSKEDLTHVVNGIETYLGSNTG-----LHFVGYIASSSEAEGGI 470
           L  + P      ++   +   L HV+N +E+ LGS+       L+F+ Y+   + +   I
Sbjct: 280 L-GVNPRFDPASSSYSLAMHSLPHVINPVESRLGSSAASLYPVLNFLLYVPELAHSPLYI 338

Query: 471 QLHTEG---NTDAFAVPRWAACL 530
           Q   +G    T+AF  PRW   +
Sbjct: 339 Q-DKDGAPVATNAFHSPRWGGIM 360
>sp|Q10351|PIGS_SCHPO GPI transamidase component PIG-S homolog
          Length = 554

 Score = 37.4 bits (85), Expect = 0.029
 Identities = 41/158 (25%), Positives = 67/158 (42%), Gaps = 10/158 (6%)
 Frame = +3

Query: 81  SLTDIPKRIRSFLTRVRTSAKYDWTFSLIVPHPKGGDSTEWDFPRIEHLYLEPLLRKLAP 260
           S T  P R  +  T V+ S +Y    SL+V      +   WD       Y  PL+ +LA 
Sbjct: 220 SSTVEPSRAETKRT-VQFSPQYRVLLSLLVGEGNH-EPINWDIENAIQKYFNPLIEQLAS 277

Query: 261 FISVKVSSQIIYGVRLERIWPSKYRDHTNRFYSKE-DLTHVVNGIETYLG-----SNTGL 422
              + + +QI Y V       ++     ++F +K  DL ++VN  E YL          +
Sbjct: 278 LAKLNIETQIQYFVE-----DAEAYIKDDKFCTKHADLPNLVNNFEKYLSFSPHIREPTI 332

Query: 423 HFVGYIASSSEAEGGIQLHTEGN----TDAFAVPRWAA 524
           HFV Y+ S       + L  E +    T++  +P+W +
Sbjct: 333 HFVLYVPSPQIQP--LWLENEDSNIIPTNSMLLPQWGS 368
>sp|Q51791|PHZE_PSEFL Anthranilate synthase, phenazine specific [Includes: Glutamine
           amidotransferase]
          Length = 637

 Score = 35.4 bits (80), Expect = 0.11
 Identities = 28/98 (28%), Positives = 56/98 (57%), Gaps = 10/98 (10%)
 Frame = +3

Query: 51  RAALEVPGKESLTD-IPKRIRSF--LTRVRT-SAKYDWT-FSLIVPHPKGGDSTEWDFPR 215
           R  L V  +++ T  I K++R+   +  VR+ S +Y +  + L++  P  G+ ++   P+
Sbjct: 436 RQVLIVDAEDTFTSMIAKQLRALGLVVTVRSFSDEYSFDGYDLVIMGPGPGNPSDVQLPK 495

Query: 216 IEHLY--LEPLLRKLAPFISVKVSSQII---YGVRLER 314
           I+HL+  +  LL +  PF++V +S Q++    G+ L+R
Sbjct: 496 IDHLHVAIRSLLNQQRPFLAVCLSHQVLSLCLGLELQR 533
>sp|Q51519|PHZB_PSECL Anthranilate synthase, phenazine specific [Includes: Glutamine
           amidotransferase]
          Length = 637

 Score = 34.3 bits (77), Expect = 0.25
 Identities = 28/98 (28%), Positives = 53/98 (54%), Gaps = 10/98 (10%)
 Frame = +3

Query: 51  RAALEVPGKESLTD-IPKRIRSF---LTRVRTSAKYDWT-FSLIVPHPKGGDSTEWDFPR 215
           R  L V  +++ T  I K++R+    +T    S +Y +  + L++  P  G+ +E   P+
Sbjct: 436 RQVLIVDAEDTFTSMIAKQLRALGLVVTVCSFSDEYSFEGYDLVIMGPGPGNPSEVQQPK 495

Query: 216 IEHLY--LEPLLRKLAPFISVKVSSQII---YGVRLER 314
           I HL+  +  LL +  PF++V +S Q++    G+ L+R
Sbjct: 496 INHLHVAIRSLLSQQRPFLAVCLSHQVLSLCLGLELQR 533
>sp|Q9JXK5|PUR4_NEIMB Phosphoribosylformylglycinamidine synthase (FGAM synthase) (FGAMS)
           (Formylglycinamide ribotide amidotransferase) (FGARAT)
           (Formylglycinamide ribotide synthetase)
          Length = 1320

 Score = 33.5 bits (75), Expect = 0.43
 Identities = 22/84 (26%), Positives = 43/84 (51%)
 Frame = +3

Query: 264 ISVKVSSQIIYGVRLERIWPSKYRDHTNRFYSKEDLTHVVNGIETYLGSNTGLHFVGYIA 443
           ++ K +S +I G ++ER +P+   +   RF+  E+ TH++  +ET+        F G   
Sbjct: 257 VAYKDNSSVIEGAKIERFYPNAAENQGYRFH--EEDTHIIMKVETHNHPTAIAPFAG--- 311

Query: 444 SSSEAEGGIQLHTEGNTDAFAVPR 515
            ++   GG ++  EG T   + P+
Sbjct: 312 -AATGAGG-EIRDEGATGKGSRPK 333
>sp|Q9F6X6|BCHN_CHLAU Light-independent protochlorophyllide reductase subunit N (LI-POR
           subunit N) (DPOR subunit N)
          Length = 444

 Score = 33.1 bits (74), Expect = 0.56
 Identities = 16/36 (44%), Positives = 20/36 (55%)
 Frame = -3

Query: 214 LGKSHSVESPPFG*GTIRENVQSYFADVRTRVRNER 107
           L  SH  E PP G GT+   +Q Y     T++RNER
Sbjct: 207 LPASHFTELPPIGPGTVIAPLQPYLHKTATQLRNER 242
>sp|P45737|CATA_BACFR Catalase
          Length = 486

 Score = 31.2 bits (69), Expect = 2.1
 Identities = 12/32 (37%), Positives = 22/32 (68%)
 Frame = -2

Query: 275 FHTDKRGEFPQQRFKIQMLDSREIPLCRISPF 180
           + + +RG+FP+ +F+IQ++   E    RI+PF
Sbjct: 245 YESIERGDFPKWKFQIQLMTEEEADNYRINPF 276
>sp|Q53RB0|LOX4_ORYSA Probable lipoxygenase 4
          Length = 877

 Score = 30.4 bits (67), Expect = 3.6
 Identities = 27/120 (22%), Positives = 51/120 (42%)
 Frame = +3

Query: 33  DLNGVYRAALEVPGKESLTDIPKRIRSFLTRVRTSAKYDWTFSLIVPHPKGGDSTEWDFP 212
           D+  +Y   L+V    +L +I KR+   L +       D    L +PH    D   W   
Sbjct: 311 DIMELYEGGLKVANASALAEIKKRVPFELIKSLLPVAGDQVLKLPLPHVIKEDKFAW--- 367

Query: 213 RIEHLYLEPLLRKLAPFISVKVSSQIIYGVRLERIWPSKYRDHTNRFYSKEDLTHVVNGI 392
           R +  +   +L  + P + +K  +       L+   P+ Y DHT++  ++  + H + G+
Sbjct: 368 RTDEEFAREMLAGVNP-VMIKRLTNFPAKSTLD---PNVYGDHTSKI-TEAHIKHNMEGL 422
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 84,113,446
Number of Sequences: 369166
Number of extensions: 1923737
Number of successful extensions: 5895
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5619
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5888
length of database: 68,354,980
effective HSP length: 105
effective length of database: 48,957,805
effective search space used: 4650991475
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)