Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_K22
(394 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex sub... 45 7e-05
sp|Q28250|SPCS2_CANFA Signal peptidase complex subunit 2 (M... 41 8e-04
sp|Q15005|SPCS2_HUMAN Signal peptidase complex subunit 2 (M... 41 8e-04
sp|Q5RAY6|SPCS2_PONPY Signal peptidase complex subunit 2 (M... 41 8e-04
sp|Q9CYN2|SPCS2_MOUSE Signal peptidase complex subunit 2 (M... 41 8e-04
sp|Q4R512|SPCS2_MACFA Signal peptidase complex subunit 2 (M... 41 8e-04
sp|Q5M8Y1|SPCS2_XENTR Probable signal peptidase complex sub... 41 0.001
sp|Q9VYY2|SPCS2_DROME Probable signal peptidase complex sub... 35 0.041
sp|O00712|NFIB_HUMAN Nuclear factor 1 B-type (Nuclear facto... 30 2.3
sp|P13622|NFIB_MESAU Nuclear factor 1 B-type (Nuclear facto... 30 2.3
>sp|Q5BJI9|SPCS2_BRARE Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 201
Score = 44.7 bits (104), Expect = 7e-05
Identities = 23/60 (38%), Positives = 31/60 (51%)
Frame = +2
Query: 2 DKPIKWNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILEKY 181
D W S +K++ D+Y L + D KQS +KS++ FFD G L MD EKY
Sbjct: 127 DPDHSWCLSSSLKRFDDQYTLRMSFTDGKTKQSRETEFTKSVSVFFDENGTLVMDQYEKY 186
>sp|Q28250|SPCS2_CANFA Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 41.2 bits (95), Expect = 8e-04
Identities = 21/53 (39%), Positives = 27/53 (50%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 175
W S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 157 WQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q15005|SPCS2_HUMAN Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 41.2 bits (95), Expect = 8e-04
Identities = 21/53 (39%), Positives = 27/53 (50%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 175
W S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 157 WQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q5RAY6|SPCS2_PONPY Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 41.2 bits (95), Expect = 8e-04
Identities = 21/53 (39%), Positives = 27/53 (50%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 175
W S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 157 WQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q9CYN2|SPCS2_MOUSE Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 41.2 bits (95), Expect = 8e-04
Identities = 21/53 (39%), Positives = 27/53 (50%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 175
W S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 157 WQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q4R512|SPCS2_MACFA Signal peptidase complex subunit 2 (Microsomal signal peptidase 25
kDa subunit) (SPase 25 kDa subunit)
Length = 226
Score = 41.2 bits (95), Expect = 8e-04
Identities = 21/53 (39%), Positives = 27/53 (50%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 175
W S +K++ DKY L + KQ +KSIA+FFD G L MD E
Sbjct: 157 WQLSSSLKRFDDKYTLKLTFISGRTKQQREAEFTKSIAKFFDHSGTLVMDAYE 209
>sp|Q5M8Y1|SPCS2_XENTR Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 201
Score = 40.8 bits (94), Expect = 0.001
Identities = 20/53 (37%), Positives = 27/53 (50%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKGNLRMDILE 175
W+ S +K++ DKY L + K +KSIA FFD G L MD+ E
Sbjct: 132 WHLSSSLKRFDDKYTLKVTYISGKTKAQRDAEFTKSIARFFDDNGTLVMDLFE 184
>sp|Q9VYY2|SPCS2_DROME Probable signal peptidase complex subunit 2 (Microsomal signal
peptidase 25 kDa subunit) (SPase 25 kDa subunit)
Length = 199
Score = 35.4 bits (80), Expect = 0.041
Identities = 17/45 (37%), Positives = 22/45 (48%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFFDIKG 151
W S M+KY DKY LT+ D + + +KS A F D G
Sbjct: 124 WEASSDMRKYDDKYLLTLSVRDTKNGKRREQSSNKSCAAFIDQNG 168
>sp|O00712|NFIB_HUMAN Nuclear factor 1 B-type (Nuclear factor 1/B) (NF1-B) (NFI-B)
(NF-I/B) (CCAAT-box binding transcription factor) (CTF)
(TGGCA-binding protein)
Length = 420
Score = 29.6 bits (65), Expect = 2.3
Identities = 15/57 (26%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFF--DIKGNLRMDILEKY 181
+N Q++ +KY K+E + +++ A + L++E I + + + LR DI ++Y
Sbjct: 32 FNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEY 88
>sp|P13622|NFIB_MESAU Nuclear factor 1 B-type (Nuclear factor 1/B) (NF1-B) (NFI-B)
(NF-I/B) (CCAAT-box binding transcription factor) (CTF)
(TGGCA-binding protein)
Length = 561
Score = 29.6 bits (65), Expect = 2.3
Identities = 15/57 (26%), Positives = 32/57 (56%), Gaps = 2/57 (3%)
Frame = +2
Query: 17 WNFQSKMKKYQDKYELTIKREDLNAKQSNLMTESKSIAEFF--DIKGNLRMDILEKY 181
+N Q++ +KY K+E + +++ A + L++E I + + + LR DI ++Y
Sbjct: 32 FNLQARKRKYFKKHEKRMSKDEERAVKDELLSEKPEIKQKWASRLLAKLRKDIRQEY 88
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 37,372,827
Number of Sequences: 369166
Number of extensions: 607895
Number of successful extensions: 1860
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 1747
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 1841
length of database: 68,354,980
effective HSP length: 96
effective length of database: 50,620,420
effective search space used: 1721094280
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)