Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_K20-1
(557 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P53033|RFC2_CHICK Activator 1 40 kDa subunit (Replicatio... 93 4e-19
sp|P35250|RFC2_HUMAN Activator 1 40 kDa subunit (Replicatio... 90 4e-18
sp|Q9WUK4|RFC2_MOUSE Activator 1 40 kDa subunit (Replicatio... 89 7e-18
sp|P53034|RFC2_DROME Activator 1 40 kDa subunit (Replicatio... 79 6e-15
sp|O94449|RFC4_SCHPO Probable activator 1 subunit 4 (Replic... 63 4e-10
sp|P40339|RFC4_YEAST Activator 1 37 kDa subunit (Replicatio... 57 4e-08
sp|Q8TSX5|RFCS_METAC Replication factor C small subunit (RF... 40 0.003
sp|Q8PVY4|RFCS_METMA Replication factor C small subunit (RF... 39 0.007
sp|Q58817|RFCS_METJA Replication factor C small subunit (RF... 33 0.48
sp|P26670|3BHS_VACCV 3 beta-hydroxysteroid dehydrogenase/de... 30 3.1
>sp|P53033|RFC2_CHICK Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
(A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
Length = 359
Score = 93.2 bits (230), Expect = 4e-19
Identities = 41/78 (52%), Positives = 58/78 (74%)
Frame = -3
Query: 468 GSGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLVKCGYACEDIVGIIFR 289
G G + SENVFKVCDEPHPL +K M+++C+ + +EA+K L HL + GY+ ED++G IFR
Sbjct: 247 GFGFINSENVFKVCDEPHPLLVKEMIQHCINANIDEAYKILAHLWRLGYSPEDVIGNIFR 306
Query: 288 VTKNHDGIPEFMKINFIK 235
V K +PE++K+ FIK
Sbjct: 307 VCKTFQ-MPEYLKLEFIK 323
Score = 39.7 bits (91), Expect = 0.005
Identities = 17/38 (44%), Positives = 27/38 (71%)
Frame = -1
Query: 209 MKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCRASA 96
+K+ EIG HM+I +G+ + LQ+ GL+ARLC+ +A
Sbjct: 317 LKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQKTA 354
>sp|P35250|RFC2_HUMAN Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
(A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
Length = 354
Score = 89.7 bits (221), Expect = 4e-18
Identities = 42/78 (53%), Positives = 56/78 (71%)
Frame = -3
Query: 468 GSGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLVKCGYACEDIVGIIFR 289
G G + SENVFKVCDEPHPL +K M+++CV + +EA+K L HL GY+ EDI+G IFR
Sbjct: 242 GFGFINSENVFKVCDEPHPLLVKEMIQHCVNANIDEAYKILAHLWHLGYSPEDIIGNIFR 301
Query: 288 VTKNHDGIPEFMKINFIK 235
V K + E++K+ FIK
Sbjct: 302 VCKTFQ-MAEYLKLEFIK 318
Score = 38.5 bits (88), Expect = 0.011
Identities = 17/39 (43%), Positives = 27/39 (69%)
Frame = -1
Query: 221 IAIIMKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCR 105
+A +K+ EIG HM+I +G+ + LQ+ GL+ARLC+
Sbjct: 308 MAEYLKLEFIKEIGYTHMKIAEGVNSLLQMAGLLARLCQ 346
>sp|Q9WUK4|RFC2_MOUSE Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
(A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
Length = 349
Score = 89.0 bits (219), Expect = 7e-18
Identities = 41/78 (52%), Positives = 56/78 (71%)
Frame = -3
Query: 468 GSGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLVKCGYACEDIVGIIFR 289
G G + SENVFKVCDEPHPL +K M+++CV + +EA+K L HL GY+ ED++G IFR
Sbjct: 237 GFGYINSENVFKVCDEPHPLLVKEMIQHCVDANIDEAYKILAHLWHLGYSPEDVIGNIFR 296
Query: 288 VTKNHDGIPEFMKINFIK 235
V K + E++K+ FIK
Sbjct: 297 VCKTFP-MAEYLKLEFIK 313
Score = 38.1 bits (87), Expect = 0.015
Identities = 16/39 (41%), Positives = 27/39 (69%)
Frame = -1
Query: 221 IAIIMKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCR 105
+A +K+ EIG HM++ +G+ + LQ+ GL+ARLC+
Sbjct: 303 MAEYLKLEFIKEIGYTHMKVAEGVNSLLQMAGLLARLCQ 341
>sp|P53034|RFC2_DROME Activator 1 40 kDa subunit (Replication factor C 40 kDa subunit)
(A1 40 kDa subunit) (RF-C 40 kDa subunit) (RFC40)
Length = 331
Score = 79.3 bits (194), Expect = 6e-15
Identities = 38/79 (48%), Positives = 55/79 (69%)
Frame = -3
Query: 471 QGSGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLVKCGYACEDIVGIIF 292
QG G +T+ENVFKVCDEPHP ++ M+ +C + ++A+K L L K GY+ EDI+ IF
Sbjct: 221 QGFGDITAENVFKVCDEPHPKLLEEMIHHCAANDIHKAYKILAKLWKLGYSPEDIIANIF 280
Query: 291 RVTKNHDGIPEFMKINFIK 235
RV K + I E +K++FI+
Sbjct: 281 RVCKRIN-IDEHLKLDFIR 298
Score = 37.0 bits (84), Expect = 0.033
Identities = 17/37 (45%), Positives = 26/37 (70%)
Frame = -1
Query: 209 MKMRHF*EIGEVHMRIVDGLATHLQLGGLIARLCRAS 99
+K+ EIG HM+I+DG+ + LQL L+A+LC A+
Sbjct: 292 LKLDFIREIGITHMKIIDGINSLLQLTALLAKLCIAA 328
>sp|O94449|RFC4_SCHPO Probable activator 1 subunit 4 (Replication factor C subunit 4)
(Replication factor C4)
Length = 342
Score = 63.2 bits (152), Expect = 4e-10
Identities = 31/78 (39%), Positives = 43/78 (55%)
Frame = -3
Query: 468 GSGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLVKCGYACEDIVGIIFR 289
G G V ENVF+V D+P P+ I ML C + A + L + G++ DIV +FR
Sbjct: 227 GFGLVNGENVFRVADQPSPVAIHAMLTACQSGNIDVALEKLQGIWDLGFSAVDIVTNMFR 286
Query: 288 VTKNHDGIPEFMKINFIK 235
V K D IPEF ++ +K
Sbjct: 287 VVKTMDSIPEFSRLEMLK 304
Score = 36.6 bits (83), Expect = 0.043
Identities = 18/35 (51%), Positives = 25/35 (71%)
Frame = -1
Query: 188 EIGEVHMRIVDGLATHLQLGGLIARLCRASAVSPE 84
EIG+ HM I++G+ T LQL GL+ RL + S + PE
Sbjct: 305 EIGQTHMIILEGVQTLLQLSGLVCRLAK-SQMKPE 338
>sp|P40339|RFC4_YEAST Activator 1 37 kDa subunit (Replication factor C subunit 4)
(Replication factor C4)
Length = 323
Score = 56.6 bits (135), Expect = 4e-08
Identities = 33/79 (41%), Positives = 46/79 (58%), Gaps = 1/79 (1%)
Frame = -3
Query: 468 GSGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSL-NHLVKCGYACEDIVGIIF 292
G G V ++NVFK+ D PHPL +K ML + S ++ + L L K GY+ DIV F
Sbjct: 215 GHGLVNADNVFKIVDSPHPLIVKKML---LASNLEDSIQILRTDLWKKGYSSIDIVTTSF 271
Query: 291 RVTKNHDGIPEFMKINFIK 235
RVTKN + E +++ IK
Sbjct: 272 RVTKNLAQVKESVRLEMIK 290
Score = 33.9 bits (76), Expect = 0.28
Identities = 13/26 (50%), Positives = 21/26 (80%)
Frame = -1
Query: 188 EIGEVHMRIVDGLATHLQLGGLIARL 111
EIG HMRI++G+ T+LQL ++A++
Sbjct: 291 EIGLTHMRILEGVGTYLQLASMLAKI 316
>sp|Q8TSX5|RFCS_METAC Replication factor C small subunit (RFC small subunit) (Clamp
loader small subunit)
Length = 338
Score = 40.4 bits (93), Expect = 0.003
Identities = 22/66 (33%), Positives = 37/66 (56%), Gaps = 1/66 (1%)
Frame = -3
Query: 459 SVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLV-KCGYACEDIVGIIFRVT 283
S++ E +++ +P IK ++E ++ F A K LN L+ + G + EDIVG I+RV
Sbjct: 224 SISRETIYRTTATANPEEIKNLIETALRGNFRIARKELNRLLYEEGLSGEDIVGQIYRVV 283
Query: 282 KNHDGI 265
D +
Sbjct: 284 SEMDNL 289
>sp|Q8PVY4|RFCS_METMA Replication factor C small subunit (RFC small subunit) (Clamp
loader small subunit)
Length = 338
Score = 39.3 bits (90), Expect = 0.007
Identities = 21/65 (32%), Positives = 36/65 (55%), Gaps = 1/65 (1%)
Frame = -3
Query: 456 VTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHLV-KCGYACEDIVGIIFRVTK 280
++ E +++ +P IK ++E ++ F A K LN L+ + G + EDIVG I+RV
Sbjct: 225 ISRETIYRTTATANPEEIKNLIETALRGNFRVARKELNRLLYEEGLSGEDIVGQIYRVVS 284
Query: 279 NHDGI 265
D +
Sbjct: 285 EMDNL 289
>sp|Q58817|RFCS_METJA Replication factor C small subunit (RFC small subunit) (Clamp loader
small subunit) [Contains: Mja RFC-1 intein; Mja RFC-2
intein; Mja RFC-3 intein]
Length = 1847
Score = 33.1 bits (74), Expect = 0.48
Identities = 23/66 (34%), Positives = 33/66 (50%), Gaps = 1/66 (1%)
Frame = -3
Query: 465 SGSVTSENVFKVCDEPHPLFIKTMLENCVQSKFNEAFKSLNHL-VKCGYACEDIVGIIFR 289
S + E V+KV P +K M+E + KF EA L L V+ G + EDI+ +FR
Sbjct: 1740 SDVIDDEIVYKVSSRARPEEVKKMMELALDGKFMEARDLLYKLMVEWGMSGEDILNQMFR 1799
Query: 288 VTKNHD 271
+ D
Sbjct: 1800 EINSLD 1805
>sp|P26670|3BHS_VACCV 3 beta-hydroxysteroid dehydrogenase/delta 5-->4-isomerase
(3Beta-HSD) [Includes: 3-beta-hydroxy-delta(5)-steroid
dehydrogenase (3-beta-hydroxy-5-ene steroid
dehydrogenase) (Progesterone reductase); Steroid
delta-isomerase (Delta-5-3-ketosteroid isomerase)]
Length = 346
Score = 30.4 bits (67), Expect = 3.1
Identities = 23/89 (25%), Positives = 40/89 (44%), Gaps = 6/89 (6%)
Frame = -3
Query: 489 YLIRLLQGSGSVTSENVFKVCDEPHPLFIKTMLENCVQ------SKFNEAFKSLNHLVKC 328
Y+++LL + V V + ++P P+ K + N +Q K EA +N ++
Sbjct: 16 YIVKLLISADDVQEIRVIDIVEDPQPITSKVKVINYIQCDINDFDKVREALDGVNLIIHT 75
Query: 327 GYACEDIVGIIFRVTKNHDGIPEFMKINF 241
A D+ G + T N E MK+N+
Sbjct: 76 A-ALVDVFG---KYTDN-----EIMKVNY 95
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 58,433,966
Number of Sequences: 369166
Number of extensions: 1099132
Number of successful extensions: 2361
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 2335
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 2359
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 3980545740
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)