Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Query= Dr_sW_027_K19 (132 letters) Database: Non-redundant SwissProt sequences 184,735 sequences; 68,354,980 total letters Score E Sequences producing significant alignments: (bits) Value sp|P14314|GLU2B_HUMAN Glucosidase II beta subunit precursor... 30 1.3 sp|Q28034|GLU2B_BOVIN Glucosidase II beta subunit precursor... 30 1.3 sp|O08795|GLU2B_MOUSE Glucosidase II beta subunit precursor... 30 2.2 sp|Q59258|CISY_BARTA Citrate synthase 28 5.0 sp|P13485|TAGF_BACSU CDP-glycerol:poly(glycerophosphate) gl... 28 8.5 sp|P14421|PA23_AGKHP Phospholipase A2, neutral (Agkistrotox... 28 8.5
>sp|P14314|GLU2B_HUMAN Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) Length = 527 Score = 30.4 bits (67), Expect = 1.3 Identities = 11/21 (52%), Positives = 13/21 (61%) Frame = +2 Query: 35 DVCGNNDQCNVGLICEEVCKE 97 D C D+ N G+ICE CKE Sbjct: 98 DCCDGTDEYNSGVICENTCKE 118
>sp|Q28034|GLU2B_BOVIN Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) (Vacuolar system associated protein 60) (VASAP-60) Length = 533 Score = 30.4 bits (67), Expect = 1.3 Identities = 10/21 (47%), Positives = 13/21 (61%) Frame = +2 Query: 35 DVCGNNDQCNVGLICEEVCKE 97 D C D+ N G++CE CKE Sbjct: 97 DCCDGTDEYNSGIVCENTCKE 117
>sp|O08795|GLU2B_MOUSE Glucosidase II beta subunit precursor (Protein kinase C substrate, 60.1 kDa protein, heavy chain) (PKCSH) (80K-H protein) Length = 521 Score = 29.6 bits (65), Expect = 2.2 Identities = 13/38 (34%), Positives = 19/38 (50%), Gaps = 2/38 (5%) Frame = +2 Query: 8 IFSSKLEE--FDVCGNNDQCNVGLICEEVCKEDNNAPK 115 I SS++ + D C D+ N G +CE C+E K Sbjct: 87 ILSSRVNDGVCDCCDGTDEYNSGTVCENTCREKGRKEK 124
>sp|Q59258|CISY_BARTA Citrate synthase Length = 322 Score = 28.5 bits (62), Expect = 5.0 Identities = 13/33 (39%), Positives = 20/33 (60%), Gaps = 2/33 (6%) Frame = -2 Query: 119 NILEHCYLLY--KLPHKLDQHYIDHCYHKHQIL 27 + LE CYLL +LP K ++H D C +H ++ Sbjct: 25 DFLESCYLLLYGELPTKQEKHDFDRCIMQHTMV 57
>sp|P13485|TAGF_BACSU CDP-glycerol:poly(glycerophosphate) glycerophosphotransferase (Polyglycerol phosphate polymerase) (CGPTase) (Major teichoic acid biosynthesis protein F) Length = 746 Score = 27.7 bits (60), Expect = 8.5 Identities = 12/41 (29%), Positives = 24/41 (58%) Frame = -1 Query: 126 LLKHFGALLSSLQTSSQIRPTLH*SLLPQTSNSSNFELKMQ 4 LLK A ++S++ + + T +LP T++ NF +K++ Sbjct: 126 LLKRLSAEVNSIERAFHVDQTTELLILPYTTDKGNFSIKVK 166
>sp|P14421|PA23_AGKHP Phospholipase A2, neutral (Agkistrotoxin) (ATX) (Phosphatidylcholine 2-acylhydrolase) Length = 122 Score = 27.7 bits (60), Expect = 8.5 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Frame = +2 Query: 8 IFSSKLEE-FDVCGNNDQCNVGLICEEVCKEDNNAPKCFKRH 130 I+S L+E + CG C E++C+ D A +CF+R+ Sbjct: 63 IYSYSLKEGYITCGKGTNCE-----EQICECDRVAAECFRRN 99
Database: Non-redundant SwissProt sequences Posted date: Dec 6, 2005 7:40 AM Number of letters in database: 68,354,980 Number of sequences in database: 184,735 Database: swissprot.01 Posted date: Dec 6, 2005 8:18 AM Number of letters in database: 66,202,850 Number of sequences in database: 184,431 Lambda K H 0.318 0.134 0.401 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,460,077 Number of Sequences: 369166 Number of extensions: 163017 Number of successful extensions: 561 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 541 Number of HSP's successfully gapped in prelim test: 0 Number of HSP's that attempted gapping in prelim test: 0 Number of HSP's gapped (non-prelim): 561 length of database: 68,354,980 effective HSP length: 17 effective length of database: 65,214,485 effective search space used: 1695576610 frameshift window, decay const: 40, 0.1 T: 12 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits)