Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_K09
(700 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9UBQ7|GRHPR_HUMAN Glyoxylate reductase/hydroxypyruvate ... 205 8e-53
sp|Q91Z53|GRHPR_MOUSE Glyoxylate reductase/hydroxypyruvate ... 203 3e-52
sp|O32264|TKRA_BACSU Probable 2-ketogluconate reductase (2KR) 139 7e-33
sp|Q9C4M5|GYAR_THELI Glyoxylate reductase (Glycolate reduct... 133 5e-31
sp|P58220|TKRA_ECO57 2-ketogluconate reductase (2KR) (2-ket... 132 1e-30
sp|P37666|TKRA_ECOLI 2-ketogluconate reductase (2KR) (2-ket... 132 1e-30
sp|O58320|GYAR_PYRHO Glyoxylate reductase (Glycolate reduct... 129 1e-29
sp|Q9UYR1|GYAR_PYRAB Glyoxylate reductase (Glycolate reduct... 127 2e-29
sp|Q8U3Y2|GYAR_PYRFU Glyoxylate reductase (Glycolate reduct... 125 1e-28
sp|Q5JEZ2|GYAR_PYRKO Glyoxylate reductase (Glycolate reduct... 123 5e-28
>sp|Q9UBQ7|GRHPR_HUMAN Glyoxylate reductase/hydroxypyruvate reductase
Length = 328
Score = 205 bits (522), Expect = 8e-53
Identities = 104/204 (50%), Positives = 136/204 (66%)
Frame = +1
Query: 4 VYSVKEGSWGAWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQA 183
+ VK G W +WKP +CGYGL S VGI+GLG IG+AIA+R+ FG ++ L +G +
Sbjct: 130 IEEVKNGGWTSWKPLWLCGYGLTQSTVGIIGLGRIGQAIARRLKPFGVQRFLYTGRQPRP 189
Query: 184 KFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNIS 363
+ E + V+ EL SDF++V+C L+ T L N + F KMK+ +F+NIS
Sbjct: 190 E-EAAEFQAEF----VSTPELAAQSDFIVVACSLTPATEGLCNKDFFQKMKETAVFINIS 244
Query: 364 RGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRNE 543
RG VV+QD LY+AL+ +IA AGLDV+ PEPLP +HPLL L NCVILPHIG AT TRN
Sbjct: 245 RGDVVNQDDLYQALASGKIAAAGLDVTSPEPLPTNHPLLTLKNCVILPHIGSATHRTRNT 304
Query: 544 MCRVAVENVIKGLQGEEMISELKL 615
M +A N++ GL+GE M SELKL
Sbjct: 305 MSLLAANNLLAGLRGEPMPSELKL 328
>sp|Q91Z53|GRHPR_MOUSE Glyoxylate reductase/hydroxypyruvate reductase
Length = 328
Score = 203 bits (517), Expect = 3e-52
Identities = 106/207 (51%), Positives = 134/207 (64%), Gaps = 3/207 (1%)
Frame = +1
Query: 4 VYSVKEGSWGAWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDT-- 177
+ VK G W +W P MCGYGL S VGIVGLG IG+AIA+R+ FG ++ L +G
Sbjct: 130 IEEVKNGGWSSWSPLWMCGYGLSQSTVGIVGLGRIGQAIARRLKPFGVQRFLYTGRQPRP 189
Query: 178 -QAKFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFV 354
+A Q E P +L SDF++VSC L+ T L + + F KMK IF+
Sbjct: 190 QEAAEFQAEFVP--------IAQLAAESDFIVVSCSLTPDTMGLCSKDFFQKMKNTAIFI 241
Query: 355 NISRGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFET 534
NISRG VV+Q+ LY+AL+ +IA AGLDV+ PEPLP HPLL L NCVILPHIG AT++T
Sbjct: 242 NISRGDVVNQEDLYQALASGQIAAAGLDVTTPEPLPPSHPLLTLKNCVILPHIGSATYKT 301
Query: 535 RNEMCRVAVENVIKGLQGEEMISELKL 615
RN M +A N++ GL+GE M SELKL
Sbjct: 302 RNTMSLLAANNLLAGLRGEAMPSELKL 328
>sp|O32264|TKRA_BACSU Probable 2-ketogluconate reductase (2KR)
Length = 325
Score = 139 bits (350), Expect = 7e-33
Identities = 75/193 (38%), Positives = 112/193 (58%)
Frame = +1
Query: 13 VKEGSWGAWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQAKFS 192
V+ G WG + + G + + +GI+G+G IG+ A+R ++L +
Sbjct: 128 VRAGKWGTVEEEALFGIDVHHQTLGIIGMGRIGEQAARRAKFGFDMEVLYHN-----RHR 182
Query: 193 QVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNISRGL 372
+ E E ++ V D LLE SDF+++ L+D+T ++ + F MK + IFVNISRG
Sbjct: 183 KQETEDSIGVKYAELDTLLEQSDFILLITPLTDETYHMIGEREFKLMKNSAIFVNISRGK 242
Query: 373 VVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRNEMCR 552
VD+ +L RAL E I GAGLDV EP+ D+PLL+L N +LPHIG AT + R MC+
Sbjct: 243 TVDEKALIRALQEGWIRGAGLDVYEKEPVTQDNPLLQLDNVTLLPHIGSATAKVRFNMCK 302
Query: 553 VAVENVIKGLQGE 591
A EN++ +QG+
Sbjct: 303 QAAENMLAAIQGQ 315
>sp|Q9C4M5|GYAR_THELI Glyoxylate reductase (Glycolate reductase)
Length = 331
Score = 133 bits (334), Expect = 5e-31
Identities = 79/197 (40%), Positives = 106/197 (53%), Gaps = 4/197 (2%)
Frame = +1
Query: 13 VKEGSWG----AWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQ 180
V+ G W W P GYGL+ +GIVG G IG+A+AKR FG K I S
Sbjct: 126 VRSGEWKKSEVGWHPLMFLGYGLKGKTLGIVGFGRIGQALAKRAKGFGMKIIYYS----- 180
Query: 181 AKFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNI 360
+ + E E + V F+ LL+ SDF+ + L+ +T ++ + MK N I +N
Sbjct: 181 -RTRKPEAEEEIGAEYVDFETLLKESDFISLHVPLTKETYHMIGEKELKLMKPNAILINT 239
Query: 361 SRGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRN 540
SRG VVD ++L +AL E IAGAGLDV EP + L KL N V+ PHIG AT E R
Sbjct: 240 SRGAVVDTNALIKALKEGWIAGAGLDVFEEEPY-YNEELFKLKNVVLAPHIGSATHEARE 298
Query: 541 EMCRVAVENVIKGLQGE 591
M + +N+I +GE
Sbjct: 299 GMAELVAKNLIAFAKGE 315
>sp|P58220|TKRA_ECO57 2-ketogluconate reductase (2KR) (2-ketoaldonate reductase)
Length = 324
Score = 132 bits (331), Expect = 1e-30
Identities = 81/193 (41%), Positives = 107/193 (55%)
Frame = +1
Query: 13 VKEGSWGAWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQAKFS 192
VK G W A G + + +GIVG+G IG A+A+R + IL + A+
Sbjct: 125 VKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYN-----ARRH 179
Query: 193 QVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNISRGL 372
E E D LL+ SDFV + L+D+T +LF F+KMK + IF+N RG
Sbjct: 180 HKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGP 239
Query: 373 VVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRNEMCR 552
VVD+++L AL + I AGLDV EPL VD PLL + N V +PHIG AT ETR M
Sbjct: 240 VVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAA 299
Query: 553 VAVENVIKGLQGE 591
AV+N+I LQG+
Sbjct: 300 CAVDNLIDALQGK 312
>sp|P37666|TKRA_ECOLI 2-ketogluconate reductase (2KR) (2-ketoaldonate reductase)
Length = 324
Score = 132 bits (331), Expect = 1e-30
Identities = 81/193 (41%), Positives = 107/193 (55%)
Frame = +1
Query: 13 VKEGSWGAWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQAKFS 192
VK G W A G + + +GIVG+G IG A+A+R + IL + A+
Sbjct: 125 VKAGEWTASIGPDWYGTDVHHKTLGIVGMGRIGMALAQRAHFGFNMPILYN-----ARRH 179
Query: 193 QVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNISRGL 372
E E D LL+ SDFV + L+D+T +LF F+KMK + IF+N RG
Sbjct: 180 HKEAEERFNARYCDLDTLLQESDFVCLILPLTDETHHLFGAEQFAKMKSSAIFINAGRGP 239
Query: 373 VVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRNEMCR 552
VVD+++L AL + I AGLDV EPL VD PLL + N V +PHIG AT ETR M
Sbjct: 240 VVDENALIAALQKGEIHAAGLDVFEQEPLSVDSPLLSMANVVAVPHIGSATHETRYGMAA 299
Query: 553 VAVENVIKGLQGE 591
AV+N+I LQG+
Sbjct: 300 CAVDNLIDALQGK 312
>sp|O58320|GYAR_PYRHO Glyoxylate reductase (Glycolate reductase)
Length = 334
Score = 129 bits (323), Expect = 1e-29
Identities = 76/197 (38%), Positives = 108/197 (54%), Gaps = 4/197 (2%)
Frame = +1
Query: 13 VKEGSWG----AWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQ 180
V+ G W AW P GY + +GI+GLG IG+AIAKR F + + S + +
Sbjct: 126 VRSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGLGRIGQAIAKRAKGFNMRILYYSRTRKE 185
Query: 181 AKFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNI 360
E+E + ++LL SDFV+++ L+ +T +L N+ MKK I +NI
Sbjct: 186 ------EVERELNAEFKPLEDLLRESDFVVLAVPLTRETYHLINEERLKLMKKTAILINI 239
Query: 361 SRGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRN 540
+RG VVD ++L +AL E IAGAGLDV EP + L KL N V+ PHIG A+F R
Sbjct: 240 ARGKVVDTNALVKALKEGWIAGAGLDVFEEEPY-YNEELFKLDNVVLTPHIGSASFGARE 298
Query: 541 EMCRVAVENVIKGLQGE 591
M + +N+I +GE
Sbjct: 299 GMAELVAKNLIAFKRGE 315
>sp|Q9UYR1|GYAR_PYRAB Glyoxylate reductase (Glycolate reductase)
Length = 335
Score = 127 bits (320), Expect = 2e-29
Identities = 73/196 (37%), Positives = 105/196 (53%), Gaps = 4/196 (2%)
Frame = +1
Query: 16 KEGSWG----AWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQA 183
+ G W AW P GY + +GI+G G IG+AIAKR F + + S
Sbjct: 128 RSGEWKKRGVAWHPKWFLGYDVYGKTIGIIGFGRIGQAIAKRARGFDMRILYYS------ 181
Query: 184 KFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNIS 363
+ + E+E + DELL SDFV+++ L+ +T ++ N+ MK+ I +N++
Sbjct: 182 RTRKPEVEKELNAEFKPLDELLRESDFVVLAVPLNKETYHMINEERLKMMKRTAILINVA 241
Query: 364 RGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRNE 543
RG V+D +L +AL E IAGAGLDV EP + L L N V+ PHIG ATF R
Sbjct: 242 RGKVIDTKALIKALKEGWIAGAGLDVYEEEPY-YNEELFSLDNVVLTPHIGSATFGAREG 300
Query: 544 MCRVAVENVIKGLQGE 591
M ++ EN+I +GE
Sbjct: 301 MAKLVAENLIAFKRGE 316
>sp|Q8U3Y2|GYAR_PYRFU Glyoxylate reductase (Glycolate reductase)
Length = 336
Score = 125 bits (314), Expect = 1e-28
Identities = 79/197 (40%), Positives = 105/197 (53%), Gaps = 4/197 (2%)
Frame = +1
Query: 13 VKEGSWG----AWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQ 180
V+ G W AW P GY L +GIVG G IG+AIA+R F + + S +
Sbjct: 126 VRSGEWKRKGIAWHPKWFLGYELYGKTIGIVGFGRIGQAIARRAKGFNMRILYYS----R 181
Query: 181 AKFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNI 360
+ SQ E E + +E+L+ SDFVI++ L+ +T + N+ MK I VNI
Sbjct: 182 TRKSQAEKELGAEYRPL--EEVLKESDFVILAVPLTKETMYMINEERLKLMKPTAILVNI 239
Query: 361 SRGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRN 540
+RG VVD +L +AL E IAGAGLDV EP + L L N V+ PHIG ATFE R
Sbjct: 240 ARGKVVDTKALIKALKEGWIAGAGLDVFEEEPY-YNEELFSLDNVVLTPHIGSATFEARE 298
Query: 541 EMCRVAVENVIKGLQGE 591
M + N+I +GE
Sbjct: 299 AMAELVARNLIAFKRGE 315
>sp|Q5JEZ2|GYAR_PYRKO Glyoxylate reductase (Glycolate reductase)
Length = 333
Score = 123 bits (308), Expect = 5e-28
Identities = 74/196 (37%), Positives = 103/196 (52%), Gaps = 4/196 (2%)
Frame = +1
Query: 16 KEGSWG----AWKPFGMCGYGLQNSVVGIVGLGNIGKAIAKRVNSFGAKKILASGSDTQA 183
+ G W AW P GY + +GIVG G IG+A+A+R FG + + S S
Sbjct: 127 RSGEWKRRGIAWHPRWFLGYDVYGKTIGIVGFGRIGQAVARRARGFGMRILYYSRS---- 182
Query: 184 KFSQVEIEPNVIVHKVAFDELLENSDFVIVSCLLSDKTRNLFNDNAFSKMKKNCIFVNIS 363
+ E E + + ++LL SDFV+++ L+ +T+ + N+ MKK I VNI+
Sbjct: 183 --RKPEAEKELGAEFRSLEDLLRESDFVVLAVPLTKETQYMINEERLRLMKKTAILVNIA 240
Query: 364 RGLVVDQDSLYRALSEERIAGAGLDVSVPEPLPVDHPLLKLHNCVILPHIGGATFETRNE 543
RG VVD +L +AL E IAGAGLDV EP + L L N V+ PHIG AT+ R
Sbjct: 241 RGKVVDTKALMKALKEGWIAGAGLDVYEEEPY-YNEELFSLKNVVLAPHIGSATYGAREG 299
Query: 544 MCRVAVENVIKGLQGE 591
M + N+I GE
Sbjct: 300 MAELVARNLIAFKNGE 315
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 78,097,122
Number of Sequences: 369166
Number of extensions: 1546939
Number of successful extensions: 5106
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4799
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4992
length of database: 68,354,980
effective HSP length: 107
effective length of database: 48,588,335
effective search space used: 6073541875
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)