Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_J13
(742 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q9V7Y2|SGPL_DROME Sphingosine-1-phosphate lyase (SP-lyas... 231 2e-60
sp|Q8CHN6|SGPL1_RAT Sphingosine-1-phosphate lyase 1 (SP-lya... 217 3e-56
sp|O95470|SGPL1_HUMAN Sphingosine-1-phosphate lyase 1 (SP-l... 215 1e-55
sp|Q8R0X7|SGPL1_MOUSE Sphingosine-1-phosphate lyase 1 (SP-l... 214 1e-55
sp|Q9C509|SGPL_ARATH Sphingosine-1-phosphate lyase (SP-lyas... 178 1e-44
sp|Q05567|SGPL_YEAST Sphingosine-1-phosphate lyase (SP-lyas... 178 1e-44
sp|Q9Y194|SGPL_CAEEL Sphingosine-1-phosphate lyase (SP-lyas... 156 5e-38
sp|Q5JJ82|MFNA_PYRKO L-tyrosine decarboxylase (TDC) 70 5e-12
sp|Q9UZD5|MFNA_PYRAB L-tyrosine decarboxylase (TDC) 66 1e-10
sp|Q8U1P6|MFNA_PYRFU L-tyrosine decarboxylase (TDC) 65 2e-10
>sp|Q9V7Y2|SGPL_DROME Sphingosine-1-phosphate lyase (SP-lyase) (SPL)
(Sphingosine-1-phosphate aldolase)
Length = 545
Score = 231 bits (589), Expect = 2e-60
Identities = 109/209 (52%), Positives = 142/209 (67%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
IS DTHKYGFAPKGSSV+LY +Y HQ V DWPGGVY SPT GSR G IIA CW+
Sbjct: 336 ISADTHKYGFAPKGSSVILYSDKKYKDHQFTVTTDWPGGVYGSPTVNGSRAGGIIAACWA 395
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
TMM FGY GY+++T+ IV T I +++I G+++ G P V+++ S+ +++ L D
Sbjct: 396 TMMSFGYDGYLEATKRIVDTARYIERGVRDIDGIFIFGKPATSVIALGSNVFDIFRLSDS 455
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKETALLMKLPGPINEGSAAIY 542
L K GW LN LQFP IH+ VT +H + GVADKFI DV+ TA +MK PG G A+Y
Sbjct: 456 LCKLGWNLNALQFPSGIHLCVTDMHTQPGVADKFIADVRSCTAEIMKDPGQPVVGKMALY 515
Query: 543 GMAQSIPDRSLVSDIAAAFIAACYSTKNQ 629
GMAQSIPDRS++ ++ F+ + Y T +Q
Sbjct: 516 GMAQSIPDRSVIGEVTRLFLHSMYYTPSQ 544
>sp|Q8CHN6|SGPL1_RAT Sphingosine-1-phosphate lyase 1 (SP-lyase) (SPL)
(Sphingosine-1-phosphate aldolase)
Length = 568
Score = 217 bits (552), Expect = 3e-56
Identities = 95/206 (46%), Positives = 146/206 (70%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
IS DTHKYG+APKGSSVV+Y + +Y +Q FV DW GG+YASP+ AGSRPG IIA CW+
Sbjct: 347 ISADTHKYGYAPKGSSVVMYSNEKYRKYQFFVDADWQGGIYASPSIAGSRPGGIIAACWA 406
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
+M FG GYV++T++I++T ++ ++NI +++LGDP++ V+++ S++ ++Y L +
Sbjct: 407 ALMHFGENGYVEATKQIIKTARFLKSELENIKNIFILGDPQLSVIALGSNDFDIYRLSNM 466
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKETALLMKLPGPINEGSAAIY 542
+ GW N LQFP SIH +T +H +K VA +F++D+++ +MK P G AIY
Sbjct: 467 MSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIY 526
Query: 543 GMAQSIPDRSLVSDIAAAFIAACYST 620
GMAQ+ DR +V++I++ F+ + YST
Sbjct: 527 GMAQATIDRKMVAEISSVFLDSLYST 552
>sp|O95470|SGPL1_HUMAN Sphingosine-1-phosphate lyase 1 (SP-lyase) (hSPL)
(Sphingosine-1-phosphate aldolase)
Length = 568
Score = 215 bits (547), Expect = 1e-55
Identities = 95/206 (46%), Positives = 146/206 (70%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
IS DTHKYG+APKGSS+VLY +Y ++Q FV DW GG+YASPT AGSRPG I A CW+
Sbjct: 347 ISADTHKYGYAPKGSSLVLYSDKKYRNYQFFVDTDWQGGIYASPTIAGSRPGGISAACWA 406
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
+M FG GYV++T++I++T ++ ++NI G++V G+P++ V+++ S + ++Y L +
Sbjct: 407 ALMHFGENGYVEATKQIIKTARFLKSELENIKGIFVFGNPQLSVIALGSRDFDIYRLSNL 466
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKETALLMKLPGPINEGSAAIY 542
+ GW LN LQFP SIH +T +H +K VA +F++D+++ +MK P G AIY
Sbjct: 467 MTAKGWNLNQLQFPPSIHFCITLLHARKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIY 526
Query: 543 GMAQSIPDRSLVSDIAAAFIAACYST 620
GMAQ+ DR++V+++++ F+ + YST
Sbjct: 527 GMAQTTVDRNMVAELSSVFLDSLYST 552
>sp|Q8R0X7|SGPL1_MOUSE Sphingosine-1-phosphate lyase 1 (SP-lyase) (mSPL)
(Sphingosine-1-phosphate aldolase)
Length = 568
Score = 214 bits (546), Expect = 1e-55
Identities = 95/206 (46%), Positives = 145/206 (70%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
IS DTHKYG+APKGSSVV+Y + +Y ++Q FV DW GGVYASP+ AGSRPG IIA CW+
Sbjct: 347 ISADTHKYGYAPKGSSVVMYSNEKYRTYQFFVGADWQGGVYASPSIAGSRPGGIIAACWA 406
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
+M FG GYV++T++I++T ++ ++NI +++ GDP++ V+++ S++ ++Y L +
Sbjct: 407 ALMHFGENGYVEATKQIIKTARFLKSELENIKNIFIFGDPQLSVIALGSNDFDIYRLSNM 466
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKETALLMKLPGPINEGSAAIY 542
+ GW N LQFP SIH +T +H +K VA +F++D+++ +MK P G AIY
Sbjct: 467 MSAKGWNFNYLQFPRSIHFCITLVHTRKRVAIQFLKDIRESVTQIMKNPKAKTTGMGAIY 526
Query: 543 GMAQSIPDRSLVSDIAAAFIAACYST 620
GMAQ+ DR LV++I++ F+ Y+T
Sbjct: 527 GMAQATIDRKLVAEISSVFLDCLYTT 552
>sp|Q9C509|SGPL_ARATH Sphingosine-1-phosphate lyase (SP-lyase) (SPL)
(Sphingosine-1-phosphate aldolase)
Length = 544
Score = 178 bits (452), Expect = 1e-44
Identities = 83/204 (40%), Positives = 128/204 (62%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
IS D HKYG APKG+S VLYR+HE HQ +W GG+Y SPT AGSRPG ++A W+
Sbjct: 343 ISVDVHKYGLAPKGTSTVLYRNHEIRKHQFVAVTEWSGGLYVSPTIAGSRPGSLVAGAWA 402
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
MM G +GY+ +T +I++ + ++ + ++ I L+V+G P + +V+ S ++++ + D
Sbjct: 403 AMMSLGEEGYLQNTSKIMEASKRLEEGVREIHELFVIGKPDMTIVAFGSKALDIFEVNDI 462
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKETALLMKLPGPINEGSAAIY 542
+ GW LN LQ P SIHI +T H V D F++D+++ + PGPI G A IY
Sbjct: 463 MSSKGWHLNALQRPNSIHICITLQHVP--VVDDFLRDLREAVETVKANPGPITGGLAPIY 520
Query: 543 GMAQSIPDRSLVSDIAAAFIAACY 614
G A +PDR +V+++ +F+ + Y
Sbjct: 521 GAAGKMPDRGMVNELLVSFMDSQY 544
>sp|Q05567|SGPL_YEAST Sphingosine-1-phosphate lyase (SP-lyase) (ySPL)
(Sphingosine-1-phosphate aldolase) (Bestowed of
sphingosine tolerance 1)
Length = 589
Score = 178 bits (452), Expect = 1e-44
Identities = 89/208 (42%), Positives = 131/208 (62%), Gaps = 4/208 (1%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
ISCDTHKYGFAPKGSSV++YR+ + HQ +V P W GG+Y SPT AGSRPG I+ CW+
Sbjct: 374 ISCDTHKYGFAPKGSSVIMYRNSDLRMHQYYVNPAWTGGLYGSPTLAGSRPGAIVVGCWA 433
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAI-KNIPGLYVLGDPKVCVVSIASDEINVYNLLD 359
TM+ G GY++S +EIV +K + I +NIP L ++G+P+ V+S +S +N++ L D
Sbjct: 434 TMVNMGENGYIESCQEIVGAAMKFKKYIQENIPDLNIMGNPRYSVISFSSKTLNIHELSD 493
Query: 360 GLRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKETALLMKLPG---PINEGS 530
L K GW N LQ P+++H+A T + V D+ I D+ + T +K P +G+
Sbjct: 494 RLSKKGWHFNALQKPVALHMAFTRLSAH--VVDE-ICDILRTTVQELKSESNSKPSPDGT 550
Query: 531 AAIYGMAQSIPDRSLVSDIAAAFIAACY 614
+A+YG+A S+ + + F+ A Y
Sbjct: 551 SALYGVAGSVKTAGVADKLIVGFLDALY 578
>sp|Q9Y194|SGPL_CAEEL Sphingosine-1-phosphate lyase (SP-lyase) (SPL)
(Sphingosine-1-phosphate aldolase)
Length = 552
Score = 156 bits (395), Expect = 5e-38
Identities = 85/206 (41%), Positives = 120/206 (58%), Gaps = 1/206 (0%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
ISCDTHKYG PKGSS+V+YRS E Q F DW GG+YA+PT AGSR G A W+
Sbjct: 341 ISCDTHKYGCTPKGSSIVMYRSKELHHFQYFSVADWCGGIYATPTIAGSRAGANTAVAWA 400
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
T++ FG YV +IV+ T + + I+ I + G V +V+ + + +N+Y + D
Sbjct: 401 TLLSFGRDEYVRRCAQIVKHTRMLAEKIEKIKWIKPYGKSDVSLVAFSGNGVNIYEVSDK 460
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKKE-TALLMKLPGPINEGSAAI 539
+ K GW LN LQ P +IHI +T + V + F D++K L K + G AA+
Sbjct: 461 MMKLGWNLNTLQNPAAIHICLTINQANEEVVNAFAVDLEKICEELAAKGEQKADSGMAAM 520
Query: 540 YGMAQSIPDRSLVSDIAAAFIAACYS 617
YGMA +P +S+V ++ A +I A YS
Sbjct: 521 YGMAAQVP-KSVVDEVIALYIDATYS 545
>sp|Q5JJ82|MFNA_PYRKO L-tyrosine decarboxylase (TDC)
Length = 384
Score = 70.5 bits (171), Expect = 5e-12
Identities = 40/160 (25%), Positives = 79/160 (49%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
I+ D HK G P + +++R ++L + P GG T G+RPG W+
Sbjct: 225 ITIDPHKMGMVPIPAGGIIFREKKFLDSISVLAPYLAGGKIWQATITGTRPGANALAVWA 284
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
+ G+ GY + +E ++ +K IPG+Y++ +P + +VS S+++ L
Sbjct: 285 MIKHLGFDGYKEVVKEKMELARWFASELKKIPGIYLIREPVLNIVSFGSEKLE--ELEKE 342
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKK 482
L+ GW ++ + + I V H K+ ++F++D+++
Sbjct: 343 LKARGWGVSAHRGYIRI---VVMPHVKREHLEEFLRDLRE 379
>sp|Q9UZD5|MFNA_PYRAB L-tyrosine decarboxylase (TDC)
Length = 384
Score = 65.9 bits (159), Expect = 1e-10
Identities = 38/160 (23%), Positives = 81/160 (50%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
I+ D HK G AP + +++R +YL + P GG T G+RPG + W+
Sbjct: 227 ITIDPHKMGMAPIPAGGIIFRRKKYLKAISVLAPYLAGGKVWQATITGTRPGASVLAVWA 286
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
+ G++GY + R+ ++ + + IK + +++ +P + +VS + N+ +
Sbjct: 287 LIKHLGFEGYREIVRKAMELSRWFAEEIKKLNNAWLVREPMLNIVSFQTK--NLRKVERE 344
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKGVADKFIQDVKK 482
L++ GW ++ + + I V H K + ++F++D+++
Sbjct: 345 LKRRGWGISAHRGYIRI---VFMPHVTKEMVEEFLRDLRE 381
>sp|Q8U1P6|MFNA_PYRFU L-tyrosine decarboxylase (TDC)
Length = 371
Score = 64.7 bits (156), Expect = 2e-10
Identities = 38/149 (25%), Positives = 72/149 (48%)
Frame = +3
Query: 3 ISCDTHKYGFAPKGSSVVLYRSHEYLSHQIFVQPDWPGGVYASPTFAGSRPGVIIATCWS 182
I+ D HK G AP + +++R +YL + P GG T G+RPG + W+
Sbjct: 226 ITIDPHKMGMAPIPAGGIVFRHKKYLRAISVLAPYLAGGKIWQATITGTRPGASVLAVWA 285
Query: 183 TMMFFGYKGYVDSTREIVQTTIKIRDAIKNIPGLYVLGDPKVCVVSIASDEINVYNLLDG 362
+ G++GY++ ++ + + IK PG +++ +P + +VS + N+ +
Sbjct: 286 LIKHLGFEGYMEIVDRAMKLSRWFAEEIKKTPGAWLVREPMLNIVSFKTK--NLRRVERE 343
Query: 363 LRKCGWQLNPLQFPLSIHIAVTHIHCKKG 449
L+ GW ++ + + I V+H C G
Sbjct: 344 LKSRGWGISAHRGYIRI---VSHASCDGG 369
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 81,483,223
Number of Sequences: 369166
Number of extensions: 1681572
Number of successful extensions: 4028
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3921
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4013
length of database: 68,354,980
effective HSP length: 108
effective length of database: 48,403,600
effective search space used: 6679696800
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)