Planarian EST Database


Dr_sW_027_H15

BLASTX 2.2.12 [Aug-07-2005]

Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.

Query= Dr_sW_027_H15
         (862 letters)

Database: Non-redundant SwissProt sequences 
           184,735 sequences; 68,354,980 total letters



                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

sp|O17847|SRA25_CAEEL  Serpentine receptor class alpha-25 (P...    37   0.054
sp|Q46089|FTSK_CAMJE  DNA translocase ftsK                         35   0.35 
sp|P62135|RAD50_NANEQ  DNA double-strand break repair rad50 ...    34   0.46 
sp|Q90069|POLG_SBMVG  Genome polyprotein [Contains: P1 prote...    34   0.46 
sp|P21231|POLG_SBMVN  Genome polyprotein [Contains: P1 prote...    34   0.46 
sp|Q9NVL8|CN105_HUMAN  Protein C14orf105                           34   0.60 
sp|O66709|Y388_AQUAE  Hypothetical protein AQ_388                  34   0.60 
sp|P97577|FEZ1_RAT  Fasciculation and elongation protein zet...    33   0.78 
sp|P52322|RPOD_MICAE  RNA polymerase sigma factor rpoD1            33   1.0  
sp|Q8IWJ2|GCC2_HUMAN  GRIP and coiled-coil domain-containing...    33   1.3  
>sp|O17847|SRA25_CAEEL Serpentine receptor class alpha-25 (Protein sra-25)
          Length = 335

 Score = 37.4 bits (85), Expect = 0.054
 Identities = 39/139 (28%), Positives = 56/139 (40%), Gaps = 24/139 (17%)
 Frame = -1

Query: 829 IRNNILNN--------VIFSSQHHMNRNI---LHQYHYN*RFSKD--SFRFHFDEKTYSF 689
           +RNNI +N         + +S  H    +   +HQ + +  ++ D  S  FHF E     
Sbjct: 50  LRNNIFSNSTQRILLVCLVNSVFHQAATLEIRIHQVYRSFVYASDPCSLLFHFTE----- 104

Query: 688 F*NCFPVIFHYQMMNYFSIF*--------LIHHIQSFDFFQYHYFV---YLIIVQN*SFS 542
              C   ++ Y + NYFS +         LI H     +F    FV    LII    SF 
Sbjct: 105 ---CEVELYFYYLTNYFSTYSVFSLTFDRLISHYNPRFYFSQQNFVSNTLLIIQCLLSFG 161

Query: 541 VYYVNIVLVPKFS*TFNCF 485
            YYV +  VP F     C+
Sbjct: 162 TYYVGLYGVPPFGYVSICY 180
>sp|Q46089|FTSK_CAMJE DNA translocase ftsK
          Length = 946

 Score = 34.7 bits (78), Expect = 0.35
 Identities = 34/139 (24%), Positives = 63/139 (45%), Gaps = 6/139 (4%)
 Frame = +1

Query: 214 ENEINKEPKSVAYDLKINDGDRIRTDVESIIKKPMPNRLRHLEPLQN----PPLLTREKL 381
           E E++  P+      K+ D  +I  D    + +P   R     P+ N    P + T+E  
Sbjct: 260 EEEVDMIPERFLKPKKLEDIKQI--DTNKNLDEPSYKRKNIEIPVSNQEVKPKIFTKELE 317

Query: 382 IRKLKSAEEKRKQMFIK*EKEILLS*YSSILFKTQNN*MFKK--ILELKRYSHSRPKTIS 555
           +R+    +EK +Q +   + EIL +     + K +         I+E  +YS + PKTI 
Sbjct: 318 LRENLIKKEKLEQEYKAYQNEILENKVKQEIKKLEEYDAINSSDIIEGNKYSFNSPKTIK 377

Query: 556 SEQLLNTQNNDIGKNQNFE 612
           +E     +++ I +N+N +
Sbjct: 378 TE---TEESDKINENKNLD 393
>sp|P62135|RAD50_NANEQ DNA double-strand break repair rad50 ATPase
          Length = 786

 Score = 34.3 bits (77), Expect = 0.46
 Identities = 43/165 (26%), Positives = 68/165 (41%), Gaps = 30/165 (18%)
 Frame = +1

Query: 67  KNNSANVPTKIIEKSK---TVISISNEHLSTCSSIKEEIETLLK--------DSGLPVSV 213
           K N   +  + +EK K    V+    +HL T  +  +EI+ +++        D  +P   
Sbjct: 443 KYNQLLIKIRALEKYKGLEKVLEAKTKHLETIENRLKEIKPIIELEIPKIELDESIPYKY 502

Query: 214 ENEINK----EPK----------SVAYDLKINDGDRIRTDVESIIKKPMPNR-----LRH 336
           +  +NK    E K             YD+ I +  RI       IKKP   R     L+ 
Sbjct: 503 KQLLNKVSYLEAKIKEYERYKNIECPYDIAIEELKRIEDKYNKYIKKPALERELNLLLKE 562

Query: 337 LEPLQNPPLLTREKLIRKLKSAEEKRKQMFIK*EKEILLS*YSSI 471
           LE   N  L  +E+++ KL    E + +   K +KE LLS  S +
Sbjct: 563 LE-RYNSLLKEKEEILSKLNPEIETKYKELTK-KKENLLSEISKV 605
>sp|Q90069|POLG_SBMVG Genome polyprotein [Contains: P1 proteinase (N-terminal protein);
            Helper component proteinase (HC-pro); Protein P3; 6 kDa
            protein 1 (6K1); Cytoplasmic inclusion protein (CI); 6
            kDa protein 2 (6K2); Viral genome-linked protein (VPg);
            Nuclear inclusion protein A (NI-a) (NIa) (NIa-pro) (49
            kDa proteinase) (49 kDa-Pro); Nuclear inclusion protein B
            (NI-b) (NIb) (RNA-directed RNA polymerase); Coat protein
            (CP)]
          Length = 3066

 Score = 34.3 bits (77), Expect = 0.46
 Identities = 19/64 (29%), Positives = 34/64 (53%)
 Frame = +1

Query: 70   NNSANVPTKIIEKSKTVISISNEHLSTCSSIKEEIETLLKDSGLPVSVENEINKEPKSVA 249
            N  ++   K++ K K   S   E +   S   +EI+++ +D  L +  + E NKE  SV+
Sbjct: 1137 NERSDAVFKVLSKLKAFFSTMGEDVKVQSL--DEIQSIDEDKKLTIDFDLETNKESSSVS 1194

Query: 250  YDLK 261
            +D+K
Sbjct: 1195 FDVK 1198
>sp|P21231|POLG_SBMVN Genome polyprotein [Contains: P1 proteinase (N-terminal protein);
            Helper component proteinase (HC-pro); Protein P3; 6 kDa
            protein 1 (6K1); Cytoplasmic inclusion protein (CI); 6
            kDa protein 2 (6K2); Viral genome-linked protein (VPg);
            Nuclear inclusion protein A (NI-a) (NIa) (NIa-pro) (49
            kDa proteinase) (49 kDa-Pro); Nuclear inclusion protein B
            (NI-b) (NIb) (RNA-directed RNA polymerase); Coat protein
            (CP)]
          Length = 3066

 Score = 34.3 bits (77), Expect = 0.46
 Identities = 19/64 (29%), Positives = 34/64 (53%)
 Frame = +1

Query: 70   NNSANVPTKIIEKSKTVISISNEHLSTCSSIKEEIETLLKDSGLPVSVENEINKEPKSVA 249
            N  ++   K++ K K   S   E +   S   +EI+++ +D  L +  + E NKE  SV+
Sbjct: 1137 NERSDAVFKVLSKLKAFFSTMGEDVKVQSL--DEIQSIDEDKKLTIDFDLETNKESSSVS 1194

Query: 250  YDLK 261
            +D+K
Sbjct: 1195 FDVK 1198
>sp|Q9NVL8|CN105_HUMAN Protein C14orf105
          Length = 296

 Score = 33.9 bits (76), Expect = 0.60
 Identities = 43/178 (24%), Positives = 75/178 (42%), Gaps = 21/178 (11%)
 Frame = +1

Query: 298 SIIKKPMPNRLRHLEPLQNPPLLTREKLI--RKLKSAEEKRKQMFIK*EKEILLS*YSSI 471
           SIIK+  P RL+ LEP+  P ++T  +L+  R+ ++  + ++ +  K +  +  S     
Sbjct: 91  SIIKRHPPQRLQKLEPIDLPRVITSGRLLSQREARTMHKAKQVLEKKMQTPMYTSENRQY 150

Query: 472 LFKTQNN*MFKK----ILELKRYSHSR-------PKTISSEQLLNT--QNNDIGKNQNFE 612
           L K Q   M +K     +ELK+  H         P+   +++ L +  +N+D        
Sbjct: 151 LHKMQVLEMIRKRQEAQMELKKSLHGEARINKQSPRDHKAKKTLQSTPRNDDHDLLTMLP 210

Query: 613 YDVXXXXXXXXXXXXXEKLQGNN------FRKMNMSFHQNENEMNLLKSASCSDIDEE 768
            ++              K Q  N        KM    H+ E +  LL  +S SD DE+
Sbjct: 211 DEILNRGPGNSKDTEFLKHQAVNNCCPWKIGKMETWLHEQEAQGQLLWDSSSSDSDEQ 268
>sp|O66709|Y388_AQUAE Hypothetical protein AQ_388
          Length = 301

 Score = 33.9 bits (76), Expect = 0.60
 Identities = 32/155 (20%), Positives = 75/155 (48%), Gaps = 24/155 (15%)
 Frame = +1

Query: 58  KVNKNNSANVPTKIIEKSKTVISISNEHLSTCSSIKEEIETLLKDSGLPVSVENEIN--- 228
           K  +N    + ++I E  KT+++++        + +  + TLL D+G+ ++ +NE++   
Sbjct: 25  KTARNLEIKITSQIPEVYKTLLNLTKVKAKVLRN-RGNVITLLLDNGIEINAKNEMSVSL 83

Query: 229 KEPKSVAYDL--------KINDGDRIRTDVESIIKKPMPN-------RLRHLEPLQNPPL 363
           +E +SV   L        K+ +G  ++     +++K + N       ++ + E  +N  +
Sbjct: 84  REGQSVVLTLVRENPLTFKLEEGKFVKNTTLEVLRKGLKNLKKFDFSQIENYENFKNSGI 143

Query: 364 LTREKLIRK-LKSAEEKRK-----QMFIK*EKEIL 450
               K+++  +K++ +  K     +  IK +KEIL
Sbjct: 144 FYENKVLKAIIKNSIDDLKVDLKYKALIKQDKEIL 178
>sp|P97577|FEZ1_RAT Fasciculation and elongation protein zeta 1 (Zygin-1) (Zygin I)
          Length = 393

 Score = 33.5 bits (75), Expect = 0.78
 Identities = 39/165 (23%), Positives = 71/165 (43%), Gaps = 21/165 (12%)
 Frame = +1

Query: 94  KIIEKSKTVISISNEHLSTCSSIKEEIETLLKDSGLPVSVENEINKEPKSVAYDLKINDG 273
           + IEKS+    I+ E L T   + EEIE ++++S            +P+     L+  DG
Sbjct: 146 EFIEKSENDSGINEEPLLTADQVIEEIEEMMQNS-----------PDPEEEVEVLEEEDG 194

Query: 274 DRIRTDVESIIKKPMP------------NRLRHLEPLQNPPLLTR---------EKLIRK 390
             I +  +S++ + M               LRH+   +   LL +         E+L+ +
Sbjct: 195 GEISSQADSVLLQEMQALTQTFNNNWSYEGLRHMSGSELTELLDQVEGAIRDFSEELVHQ 254

Query: 391 LKSAEEKRKQMFIK*EKEILLS*YSSILFKTQNN*MFKKILELKR 525
           L   +E      ++ EKE+  S + ++L + QN    ++ L  KR
Sbjct: 255 LARRDE------LEFEKEVKNS-FITVLIEVQNKQKEQRELMKKR 292
>sp|P52322|RPOD_MICAE RNA polymerase sigma factor rpoD1
          Length = 416

 Score = 33.1 bits (74), Expect = 1.0
 Identities = 28/108 (25%), Positives = 50/108 (46%), Gaps = 2/108 (1%)
 Frame = +1

Query: 91  TKIIEKSKTVISISNEHLSTCSSIKEEIETLLKDSGLPVSVENEINKEPKSVAYDLKIND 270
           TKI+ +         E  +      E++  + K + LP+S+E  I KE  S   D    D
Sbjct: 272 TKILSQEMRRKPTEEEIATKMEMTIEKLRFIAKSAQLPISLETPIGKEEDSRLGDFIEAD 331

Query: 271 GDRIRTDV-ESIIKKPMPNRLRHLEPLQNPPLLTREKL-IRKLKSAEE 408
           G+    +V ++++++ + N L  L P +   L  R  L   ++K+ EE
Sbjct: 332 GETPEDEVSKNLLREDLENVLDTLSpreRDVLRLRYGLDDGRMKTLEE 379
>sp|Q8IWJ2|GCC2_HUMAN GRIP and coiled-coil domain-containing protein 2 (Golgi coiled coil
           protein GCC185) (CTCL tumor antigen se1-1)
           (CLL-associated antigen KW-11)
          Length = 1583

 Score = 32.7 bits (73), Expect = 1.3
 Identities = 33/129 (25%), Positives = 55/129 (42%), Gaps = 3/129 (2%)
 Frame = +1

Query: 67  KNNSANVPTKIIEKSKTVISISNEHLSTCSSIKEEIETLLKDSGLPVSVENEI-NKEPKS 243
           K   A+   ++ +K +T  +  +  L T + ++ E E LL    L   +EN I N + K+
Sbjct: 408 KQQQASDVHELQQKLRTAFTEKDALLETVNRLQGENEKLLSQQELVPELENTIKNLQEKN 467

Query: 244 VAYDLKINDGDRIRTDVESIIKKPMPNRLRHLEPLQNPPLLTREK--LIRKLKSAEEKRK 417
             Y L ++  D +  ++E  I                   LT EK   I KLK++ E+  
Sbjct: 468 GVYLLSLSQRDTMLKELEGKINS-----------------LTEEKDDFINKLKNSHEEMD 510

Query: 418 QMFIK*EKE 444
               K E+E
Sbjct: 511 NFHKKCERE 519
  Database: Non-redundant SwissProt sequences
    Posted date:  Dec 6, 2005  7:40 AM
  Number of letters in database: 68,354,980
  Number of sequences in database:  184,735
  
  Database: swissprot.01
    Posted date:  Dec 6, 2005  8:18 AM
  Number of letters in database: 66,202,850
  Number of sequences in database:  184,431
  
Lambda     K      H
   0.318    0.134    0.401 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 77,814,152
Number of Sequences: 369166
Number of extensions: 1392559
Number of successful extensions: 4388
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 4209
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4383
length of database: 68,354,980
effective HSP length: 109
effective length of database: 48,218,865
effective search space used: 8534739105
frameshift window, decay const: 40,  0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)