Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_G13-2
(565 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|Q27928|GPDA_DROPS Glycerol-3-phosphate dehydrogenase [NA... 225 7e-59
sp|P13707|GPDA_MOUSE Glycerol-3-phosphate dehydrogenase [NA... 224 1e-58
sp|Q27567|GPDA_DROEZ Glycerol-3-phosphate dehydrogenase [NA... 223 3e-58
sp|P13706|GPDA_DROME Glycerol-3-phosphate dehydrogenase [NA... 223 3e-58
sp|Q27556|GPDA_DROAE Glycerol-3-phosphate dehydrogenase [NA... 223 3e-58
sp|P07735|GPDA_DROVI Glycerol-3-phosphate dehydrogenase [NA... 222 4e-58
sp|O97463|GPDA_DROKA Glycerol-3-phosphate dehydrogenase [NA... 222 4e-58
sp|P21695|GPDA_HUMAN Glycerol-3-phosphate dehydrogenase [NA... 220 2e-57
sp|O35077|GPDA_RAT Glycerol-3-phosphate dehydrogenase [NAD+... 219 5e-57
sp|O57656|GPDA_FUGRU Glycerol-3-phosphate dehydrogenase [NA... 218 8e-57
>sp|Q27928|GPDA_DROPS Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 353
Score = 225 bits (573), Expect = 7e-59
Identities = 109/186 (58%), Positives = 139/186 (74%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG +D G L+ LFQ ++FR+VV+ D E+CGALKNIVA GAGF +GL GDNTK
Sbjct: 167 TIGCTDKKYGKVLRDLFQANHFRVVVVDDADAVEVCGALKNIVACGAGFVDGLKLGDNTK 226
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI+F + F+ G +TF ESCGVADLITTCYGGRN+R+ +A V +GK+
Sbjct: 227 AAVIRLGLMEMIRFVDVFYP--GSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKT 284
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ +LE E+LNGQ QGP T++EVN +L K E++FPLFTAI+ IC ++ P IDC+R
Sbjct: 285 IEELEKEMLNGQKLQGPPTAEEVNYMLKNKKLEDKFPLFTAIHKICTNQLKPKDLIDCIR 344
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 345 NHPEHM 350
>sp|P13707|GPDA_MOUSE Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 349
Score = 224 bits (570), Expect = 1e-58
Identities = 108/186 (58%), Positives = 137/186 (73%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG DPA+G LK L QT FR+ V+++ E+CGALKNIVA+GAGF +GLG+GDNTK
Sbjct: 165 TIGCKDPAQGQLLKDLMQTPNFRITVVQEVDTVEICGALKNIVAVGAGFCDGLGFGDNTK 224
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI FA F ++ TFLESCGVADLITTCYGGRN+++ +A TGKS
Sbjct: 225 AAVIRLGLMEMIAFAKLFCSGT-VSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKS 283
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ QLE E+LNGQ QGP T++E++ +L ++FPLFTA+Y +C P +FI CL+
Sbjct: 284 IEQLEKEMLNGQKLQGPQTARELHSILQHKGLVDKFPLFTAVYKVCYEGQPVGEFIRCLQ 343
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 344 NHPEHM 349
>sp|Q27567|GPDA_DROEZ Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 350
Score = 223 bits (568), Expect = 3e-58
Identities = 108/186 (58%), Positives = 139/186 (74%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG +D G L+ LFQ ++FR+VV++D E+CGALKNIVA GAGF +GL GDNTK
Sbjct: 167 TIGCTDKKYGKVLRDLFQANHFRVVVVEDADAVEVCGALKNIVACGAGFVDGLKLGDNTK 226
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI+F + F+ G +TF ESCGVADLITTCYGGRN+R+ +A V +GK+
Sbjct: 227 AAVIRLGLMEMIRFVDVFYP--GSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKT 284
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ +LE E+LNGQ QGP T++EVN +L E++FPLFTAI+ IC ++ P IDC+R
Sbjct: 285 IEELEKEMLNGQKLQGPPTAEEVNYMLKNKGLEDKFPLFTAIHKICTNQLKPKDLIDCIR 344
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 345 NHPEHM 350
>sp|P13706|GPDA_DROME Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 363
Score = 223 bits (567), Expect = 3e-58
Identities = 108/186 (58%), Positives = 139/186 (74%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG +D G L+ LFQ ++FR+VV+ D E+CGALKNIVA GAGF +GL GDNTK
Sbjct: 167 TIGCTDKKYGKVLRDLFQANHFRVVVVDDADAVEVCGALKNIVACGAGFVDGLKLGDNTK 226
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI+F + F+ G +TF ESCGVADLITTCYGGRN+R+ +A V +GK+
Sbjct: 227 AAVIRLGLMEMIRFVDVFYP--GSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKT 284
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ +LE E+LNGQ QGP T++EVN +L E++FPLFTAI+ IC ++ P+ IDC+R
Sbjct: 285 IEELEKEMLNGQKLQGPPTAEEVNYMLKNKGLEDKFPLFTAIHKICTNQLKPNDLIDCIR 344
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 345 NHPEHM 350
>sp|Q27556|GPDA_DROAE Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 350
Score = 223 bits (567), Expect = 3e-58
Identities = 108/186 (58%), Positives = 138/186 (74%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG +D G L+ LFQ ++FR+VV++D E+CGALKNIVA GAGF +GL GDNTK
Sbjct: 167 TIGCTDKKYGKVLRDLFQANHFRVVVVEDAEAVEVCGALKNIVACGAGFVDGLKLGDNTK 226
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI+F + F+ G +TF ESCGVADLITTCYGGRN+R+ +A V +GK+
Sbjct: 227 AAVIRLGLMEMIRFVDVFYP--GSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKT 284
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ LE E+LNGQ QGP T++EVN +L E++FPLFTAI+ IC ++ P IDC+R
Sbjct: 285 IEDLEKEMLNGQKLQGPPTAEEVNYMLKNKGLEDKFPLFTAIHKICTNQLKPKDLIDCIR 344
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 345 NHPEHM 350
>sp|P07735|GPDA_DROVI Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 353
Score = 222 bits (566), Expect = 4e-58
Identities = 108/186 (58%), Positives = 138/186 (74%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG +D G L+ LFQ ++FR+VV++D E+CGALKNIVA GAGF +GL GDNTK
Sbjct: 167 TIGCTDKKYGKVLRDLFQANHFRVVVVEDADAVEVCGALKNIVACGAGFVDGLKLGDNTK 226
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI+F + F+ G +TF ESCGVADLITTCYGGRN+R+ +A V +GK+
Sbjct: 227 AAVIRLGLMEMIRFVDVFYP--GSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKT 284
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ LE E+LNGQ QGP T++EVN +L E++FPLFTAI+ IC ++ P IDC+R
Sbjct: 285 IEDLEKEMLNGQKLQGPPTAEEVNYMLKNKGLEDKFPLFTAIHKICTNQLKPKDLIDCIR 344
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 345 NHPEHM 350
>sp|O97463|GPDA_DROKA Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 360
Score = 222 bits (566), Expect = 4e-58
Identities = 108/186 (58%), Positives = 139/186 (74%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG +D G L+ LFQ ++FR+VV++D E+CGALKNIVA GAGF +GL GDNTK
Sbjct: 167 TIGCTDKKYGKVLRDLFQANHFRVVVVEDADAVEVCGALKNIVACGAGFVDGLKLGDNTK 226
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI+F + F+ G +TF ESCGVADLITTCYGGRN+R+ +A V +GK+
Sbjct: 227 AAVIRLGLMEMIRFVDVFYP--GSKLSTFFESCGVADLITTCYGGRNRRVSEAFVTSGKT 284
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ +LE E+LNGQ QGP T++EVN +L E++FPLFTAI+ IC ++ P IDC+R
Sbjct: 285 IEELEKEMLNGQKLQGPPTAEEVNYMLKNKGLEDKFPLFTAIHKICINQLKPKDLIDCIR 344
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 345 NHPEHM 350
>sp|P21695|GPDA_HUMAN Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 349
Score = 220 bits (561), Expect = 2e-57
Identities = 107/186 (57%), Positives = 135/186 (72%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG DPA+G LK L QT FR+ V+++ E+CGALKN+VA+GAGF +GLG+GDNTK
Sbjct: 165 TIGCKDPAQGQLLKELMQTPNFRITVVQEVDTVEICGALKNVVAVGAGFCDGLGFGDNTK 224
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI FA F ++ TFLESCGVADLITTCYGGRN+++ +A TGKS
Sbjct: 225 AAVIRLGLMEMIAFAKLFCSGP-VSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKS 283
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ QLE ELLNGQ QGP T++E+ +L ++FPLF A+Y +C P +FI CL+
Sbjct: 284 IEQLEKELLNGQKLQGPETARELYSILQHKGLVDKFPLFMAVYKVCYEGQPVGEFIHCLQ 343
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 344 NHPEHM 349
>sp|O35077|GPDA_RAT Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 349
Score = 219 bits (557), Expect = 5e-57
Identities = 106/186 (56%), Positives = 135/186 (72%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG DPA+G LK L QT FR+ V+++ E+CGALKNIVA+GAGF +GLG+GDNTK
Sbjct: 165 TIGCKDPAQGQLLKDLMQTPNFRITVVQEVDTVEICGALKNIVAVGAGFCDGLGFGDNTK 224
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI A F ++ TFLESCGVADLITTCYGGRN+++ +A TGKS
Sbjct: 225 AAVIRLGLMEMIASAKLFCSG-SVSSATFLESCGVADLITTCYGGRNRKVAEAFARTGKS 283
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ QLE E+LNGQ QGP T++E++ +L ++FPLFTA+Y +C P + I CL+
Sbjct: 284 IEQLEKEMLNGQKLQGPQTARELHSILQHKGLVDKFPLFTAVYKVCYEGQPVGEIIRCLQ 343
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 344 NHPEHM 349
>sp|O57656|GPDA_FUGRU Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic (GPD-C)
(GPDH-C)
Length = 351
Score = 218 bits (555), Expect = 8e-57
Identities = 107/186 (57%), Positives = 135/186 (72%)
Frame = +2
Query: 2 TIGSSDPARGLELKTLFQTSYFRMVVIKDEVGCELCGALKNIVALGAGFSEGLGYGDNTK 181
TIG + + G LK L QT FR+ V+++ E+CGALKNIVA+GAGF +GLG+GDNTK
Sbjct: 166 TIGCKNKSHGPLLKELMQTINFRVTVVEEYDVVEICGALKNIVAVGAGFCDGLGFGDNTK 225
Query: 182 AAIIRLGFMEMIKFANTFFGNRGINQTTFLESCGVADLITTCYGGRNKRIGKALVETGKS 361
AA+IRLG MEMI FA F ++ TFLESCGVADLITTCYGGRN+++ KA V+TGKS
Sbjct: 226 AAVIRLGLMEMIAFARIFCTAGPVSSATFLESCGVADLITTCYGGRNRKVAKAFVKTGKS 285
Query: 362 VPQLELELLNGQSAQGPLTSKEVNQVLAAHKKENEFPLFTAIYNICEGKMPPSKFIDCLR 541
+ +LE E+L GQ QGP T+ EV ++L ++FPLF A+Y IC P S+FI CL+
Sbjct: 286 IEELEKEMLEGQKLQGPATAAEVYRILKHKNLIDKFPLFNAVYQICYQGHPVSEFISCLQ 345
Query: 542 NHPEHM 559
NHPEHM
Sbjct: 346 NHPEHM 351
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 66,224,629
Number of Sequences: 369166
Number of extensions: 1317607
Number of successful extensions: 4190
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 3919
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 4067
length of database: 68,354,980
effective HSP length: 104
effective length of database: 49,142,540
effective search space used: 4078830820
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)