Reference:
Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schäffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Query= Dr_sW_027_G05
(868 letters)
Database: Non-redundant SwissProt sequences
184,735 sequences; 68,354,980 total letters
Score E
Sequences producing significant alignments: (bits) Value
sp|P54675|PI3K3_DICDI Phosphatidylinositol 3-kinase 3 (PI3-... 37 0.071
sp|P20134|SFL1_YEAST Flocculation suppression protein (SFL1... 33 1.0
sp|P53185|MTC2_YEAST Protein MTC2 32 3.0
sp|O94166|CREA_ASPAC DNA-binding protein creA (Carbon catab... 31 3.9
sp|P39104|PIK1_YEAST Phosphatidylinositol 4-kinase PIK1 (PI... 31 5.1
sp|Q88VK7|IF2_LACPL Translation initiation factor IF-2 31 5.1
sp|P18899|DDR48_YEAST Stress protein DDR48 (DNA damage-resp... 30 6.6
sp|P70039|APC_XENLA Adenomatous polyposis coli homolog (APC... 30 6.6
sp|P09547|SWI1_YEAST Transcription regulatory protein SWI1 ... 30 8.7
sp|O95171|SCEL_HUMAN Sciellin 30 8.7
>sp|P54675|PI3K3_DICDI Phosphatidylinositol 3-kinase 3 (PI3-kinase) (PtdIns-3-kinase)
(PI3K)
Length = 1585
Score = 37.0 bits (84), Expect = 0.071
Identities = 51/260 (19%), Positives = 96/260 (36%), Gaps = 1/260 (0%)
Frame = +2
Query: 8 EQSEHFKSELNKYEECKSSLSSRSNYYXXXXXXXXXXRLTTSQNRNTSCMSTSNSLGTIS 187
E +E FK N + K + + SN Y + N N + + +N+ +
Sbjct: 21 ESTEQFKQWSNLIKNIKENSLNNSNIYLTIPTTQNLINNNNNNNNNNNNNNNNNN----N 76
Query: 188 CSNSNFSNLTFASTAMSNLDXXXXXXXXXXXXXXTVQSTGKSNNLTRNLSHDETFSKAPP 367
+N+N +N+ S + N + + +NN NLS D + +K
Sbjct: 77 NNNNNNNNVIIPSASTENKEEN--------------DNNNSNNNNNINLSPDSSITKDIN 122
Query: 368 INKNRVGSPNNTLEKSPGNKTQFISIS-NTGGIRQDSKPDMNKLNKYSFGTGNHYXXXXX 544
I +N++ T K T + S + G I KP+ + N+ T N+
Sbjct: 123 ITENKITEIKTTETKETSTGTSPLEKSPSKGFIISPKKPE--EENEIEGETINNIAITNY 180
Query: 545 XXXXXXXXSSYANKTNQQGRMSNMNASVGCPKNISPNSYETHKNSARINGDAEAGKPHRV 724
N + +N N + N + N+ ++ N+ I+ + P +
Sbjct: 181 TQGPSMLTLMKKKLENIKKNNNNNNNNGNGNNNSNNNNSNSNNNNNGIS--PSSSPPSHL 238
Query: 725 EVRVPDNARHMSNSGNTTDS 784
+N + +NS NTT++
Sbjct: 239 NGNNNNNNSNNTNSNNTTNA 258
>sp|P20134|SFL1_YEAST Flocculation suppression protein (SFL1 protein)
Length = 766
Score = 33.1 bits (74), Expect = 1.0
Identities = 24/124 (19%), Positives = 49/124 (39%), Gaps = 2/124 (1%)
Frame = +2
Query: 332 LSHDETFSKAPPINKNRVGSPNNTLEKSPGNKTQFISISNTGGIRQDSKPDMNKLNKYSF 511
+SHD + + A + +NT + + GNK + GG+++ K + K+ ++
Sbjct: 127 VSHDHSSNDANSGDDANTNDDSNTHDDNSGNKNSSGDENTGGGVQEKEKSNPTKIWEFKH 186
Query: 512 GTGNHYXXXXXXXXXXXXXSSYANKT--NQQGRMSNMNASVGCPKNISPNSYETHKNSAR 685
+G +S N + N + SN N + + P+S + S+
Sbjct: 187 SSGIFKKGDIEGLKHIKRRASSRNNSSINSRKNSSNQNYDIDSGARVRPSSIQDPSTSSN 246
Query: 686 INGD 697
G+
Sbjct: 247 SFGN 250
>sp|P53185|MTC2_YEAST Protein MTC2
Length = 909
Score = 31.6 bits (70), Expect = 3.0
Identities = 26/115 (22%), Positives = 46/115 (40%), Gaps = 4/115 (3%)
Frame = +2
Query: 122 LTTSQNRNTSCMSTSNSLGTISCSNSNFSNLTFASTAMSNLDXXXXXXXXXXXXXXTVQS 301
++T N TS ++ N + S + N N T +S D T +
Sbjct: 699 ISTPPNTITSASTSDNCTSSQSAAQKNIENFT----TLSKYDVYSSTSFIPSTSKNTNTN 754
Query: 302 TGKSNNLTRNL----SHDETFSKAPPINKNRVGSPNNTLEKSPGNKTQFISISNT 454
K +N S+ F+K+ I++ GS NN+ + PG+ + S+ N+
Sbjct: 755 VSKQQQQPQNSTPCSSNRFLFNKSSLISQESNGSNNNSGTQGPGSMNESYSLDNS 809
>sp|O94166|CREA_ASPAC DNA-binding protein creA (Carbon catabolite repressor)
Length = 431
Score = 31.2 bits (69), Expect = 3.9
Identities = 13/36 (36%), Positives = 24/36 (66%)
Frame = +2
Query: 311 SNNLTRNLSHDETFSKAPPINKNRVGSPNNTLEKSP 418
++N++R+ SH+E S + + ++R SPN+T SP
Sbjct: 269 THNMSRSHSHEEDDSYSHRVKRSRPNSPNSTAPSSP 304
>sp|P39104|PIK1_YEAST Phosphatidylinositol 4-kinase PIK1 (PI4-kinase) (PtdIns-4-kinase)
Length = 1066
Score = 30.8 bits (68), Expect = 5.1
Identities = 33/113 (29%), Positives = 46/113 (40%), Gaps = 6/113 (5%)
Frame = +2
Query: 371 NKNRVGSPNNTLEKSPGNKTQFISISNTGGIRQDSKPDMNKLNKYSFGTGNHYXXXXXXX 550
N NR GS +NT + GNK +IS R D + N++ TG
Sbjct: 306 NINRDGSISNTAKALDGNKGDYISPKG----RND---ENNEIGNNEDETGGETEEDADAL 358
Query: 551 XXXXXXSSYANKTNQQGRMSNMNAS--VGCPK----NISPNSYETHKNSARIN 691
SS + N Q R S+ +++ G PK N P S + KNS + N
Sbjct: 359 NSDHFTSSMPDLHNIQPRTSSASSASLEGTPKLNRTNSQPLSRQAFKNSKKAN 411
>sp|Q88VK7|IF2_LACPL Translation initiation factor IF-2
Length = 858
Score = 30.8 bits (68), Expect = 5.1
Identities = 32/116 (27%), Positives = 47/116 (40%), Gaps = 2/116 (1%)
Frame = +2
Query: 125 TTSQNRNTSC-MSTSNSLGTISCSNSNFSNLTFASTAMSNLDXXXXXXXXXXXXXXTVQS 301
T S N N S S SNS SN+N SN + +N + S
Sbjct: 123 TNSNNGNRSANASRSNSARNNGGSNTNRSNNNNNNNRSNNNNRSNTSNNR-------TNS 175
Query: 302 TG-KSNNLTRNLSHDETFSKAPPINKNRVGSPNNTLEKSPGNKTQFISISNTGGIR 466
TG ++NN +N +++ T + N NR NN+ G ++ +N GG R
Sbjct: 176 TGNRTNNGGQNRNNNRTTTNNNNNNSNRSNGSNNSSRNGSGRFGGSLNSNNNGGGR 231
>sp|P18899|DDR48_YEAST Stress protein DDR48 (DNA damage-responsive protein 48) (DDRP 48)
(YP 75) (Flocculent-specific protein)
Length = 430
Score = 30.4 bits (67), Expect = 6.6
Identities = 36/135 (26%), Positives = 49/135 (36%), Gaps = 8/135 (5%)
Frame = +2
Query: 299 STGKSNNLTRNLSHDETFSKAPPINKNRVGSPNNTLEKSPGNKTQFISISNT----GGIR 466
S G +N+ + S+ S N + GS NN NK + SN G
Sbjct: 265 SYGSNNDDSYGSSNKNKSSYGSSSNDDSYGSSNNDDSYGSSNKKKSSYGSNNDDSYGSNN 324
Query: 467 QDSKPDMNKLNKYSFGTGNH----YXXXXXXXXXXXXXSSYANKTNQQGRMSNMNASVGC 634
DS NK K S+G+ N+ SSY + + SN N S G
Sbjct: 325 DDSYGSSNK-KKSSYGSSNNDSYGSNNDDSYGSSNKKKSSYGSNNDDSYGSSNNNDSYGS 383
Query: 635 PKNISPNSYETHKNS 679
+ S S +KNS
Sbjct: 384 NNDDSYGSSNRNKNS 398
>sp|P70039|APC_XENLA Adenomatous polyposis coli homolog (APC protein)
Length = 2829
Score = 30.4 bits (67), Expect = 6.6
Identities = 16/42 (38%), Positives = 24/42 (57%), Gaps = 3/42 (7%)
Frame = +2
Query: 710 KPHRVEVRVPDNARHMS---NSGNTTDSQDVEYGTPDSPVSP 826
KP+RV+ ++ D H S +SGN+ Q+ + P SPV P
Sbjct: 1739 KPYRVK-KIMDQINHTSAATSSGNSRSMQETDKNKPTSPVKP 1779
>sp|P09547|SWI1_YEAST Transcription regulatory protein SWI1 (SWI/SNF complex component
SWI1) (Transcription regulatory protein ADR6)
(Regulatory protein GAM3)
Length = 1314
Score = 30.0 bits (66), Expect = 8.7
Identities = 32/156 (20%), Positives = 62/156 (39%), Gaps = 3/156 (1%)
Frame = +2
Query: 317 NLTRNLSHDETFSKAPPINKNRVGSPNNTLEKSPGNKTQFISISNTGGIRQDSKPDMNKL 496
NL N +++ T + N N + N +P N T + +N+ G ++ + N
Sbjct: 5 NLNNNNNNNNTTTTTTTTNNNNTNNNNTNNNNNPANNT---NNNNSTGHSSNTNNNTNNN 61
Query: 497 NKYSFGTG-NHYXXXXXXXXXXXXXSSYANKTNQQGRMSNMNASVGCPKNI-SPNSYETH 670
N + +G + + S N +N +N + +V N SP + +
Sbjct: 62 NTNTGASGVDDFQNFFDPKPFDQNLDSNNNNSNSNNNDNNNSNTVASSTNFTSPTAVVNN 121
Query: 671 KNSARINGDAEAGKPHRVEVRVPD-NARHMSNSGNT 775
A + G A + ++ + P N+ + SN+ NT
Sbjct: 122 AAPANVTGGKAA---NFIQNQSPQFNSPYDSNNSNT 154
>sp|O95171|SCEL_HUMAN Sciellin
Length = 668
Score = 30.0 bits (66), Expect = 8.7
Identities = 30/117 (25%), Positives = 47/117 (40%), Gaps = 6/117 (5%)
Frame = +2
Query: 311 SNNLTRNLSHDETFSKAPPINKNRVGSP--NNTLEKSPGNKTQFISISNTGGIRQDS--- 475
+ N+TR S D P + ++ GS NN ++ PG + S GG DS
Sbjct: 361 NKNITRGQSLDNLIKVTPEVKRSNQGSKDLNNFIKVYPGTEK-----STEGGQSLDSLIK 415
Query: 476 -KPDMNKLNKYSFGTGNHYXXXXXXXXXXXXXSSYANKTNQQGRMSNMNASVGCPKN 643
P+ N+ N+ + N ANK+++QG ++N S KN
Sbjct: 416 VTPERNRTNQGNQDLENLIKVIPS-----------ANKSSEQGLDEHINVSPKAVKN 461
Database: Non-redundant SwissProt sequences
Posted date: Dec 6, 2005 7:40 AM
Number of letters in database: 68,354,980
Number of sequences in database: 184,735
Database: swissprot.01
Posted date: Dec 6, 2005 8:18 AM
Number of letters in database: 66,202,850
Number of sequences in database: 184,431
Lambda K H
0.318 0.134 0.401
Gapped
Lambda K H
0.267 0.0410 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 96,546,406
Number of Sequences: 369166
Number of extensions: 2031429
Number of successful extensions: 5336
Number of sequences better than 10.0: 10
Number of HSP's better than 10.0 without gapping: 5104
Number of HSP's successfully gapped in prelim test: 0
Number of HSP's that attempted gapping in prelim test: 0
Number of HSP's gapped (non-prelim): 5320
length of database: 68,354,980
effective HSP length: 110
effective length of database: 48,034,130
effective search space used: 8550075140
frameshift window, decay const: 40, 0.1
T: 12
A: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)